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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFA158
Library AF
(Link to library)
Clone ID AFA158
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15789-1
Original site URL
Representative seq. ID AFA158P
(Link to Original site)
Representative DNA sequence 
>AFA158 (AFA158Q) /CSM/AF/AFA1-C/AFA158Q.Seq.d/
GGTTTTACAACACCTTATAATCAATCAAATTCATCACCAGTTTCATCAATTGGTTCAAAT
TCATCTAGGAATAGTCGTTTACCAAATACTTCAGTTTCAATACCTGGATTATATAGTAGT
GATAATGATAATACTCGATTAAAAAATGTAAATAATAATAATAATAACAACAATAATACA
ACAACATATGGATCATCAACATTCCCAAGTTCAGTTATATCAACAACTGGTAGTATAAGT
AATTCAATTGCAAGTGCAATGGATAATGATAGTAGTTATAGTAATGAATCATCACCAACT
TCTTCAATGACATTATTACCAACTACTACTACTACAACAACAACAACCACTACCACAGCA
ACAACAACAGACTCTACTAATAATAATAACAATAATGCAACGGTAGTAATTGGAAAAGGA
AAACCACCAAAAGAACCAAAACCTGTAAAACCACCTAAAGAACCAAAACCACCAAAAGAA
CCAAAACCAXXXXXXXXXXCANTCTCATACTATGCAGTTTATGATGGTCATGGTGGTACT
GAAACCTCAACACTCTTGGAGCCAACAGTTCATAATTGTTTGGTCAATTCTCAAAGTTTC
CGAGATGGTGACTATGAACAAGCTTTCCGTGATGCATACGCTGAAGCTGATGATATTGTA
ATTGAAAAATGTGAAAAGAGTGGTAGCACAGGTGTATCGGCTCTATTGGTTGGAAATAAA
CTCTACACTGCAAATGTAGGCGATTCAGAGATAGTTTTAGCACGTGCTCAACCAAATGCA
AATCCTAAAGGACCCGTCACCTATGAACCGGTTTTACTCTCCTACAAACATTTGGCAAGT
GATGACCAAGAAAAGAAGAGAGTCACCGATTTGGGTGGTATGATCATTTTCAATCGTTTG
TTTGGTTCATTGGCTGTTAGTAGATCGTTTGGTGATAAGGAGTACAAAGAGGGTGAAAAG
AAGTTTTGTGTCTCTGATCCTTATCAAACAACAACCGATCTCACTGCTCGTGATCATTTC
TTCATTTTGGCTTGTGATGGTNCTTTGGGATAAGGTCGAATATGATGAAGCCGTTCAATT
TGTTCAAAGAAATATAAAATTGGGAAAAAGTGCAACTGAAATTTCAGAATTATTAGCTCA
AGATTNCTTAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
GFTTPYNQSNSSPVSSIGSNSSRNSRLPNTSVSIPGLYSSDNDNTRLKNVNNNNNNNNNT
TTYGSSTFPSSVISTTGSISNSIASAMDNDSSYSNESSPTSSMTLLPTTTTTTTTTTTTA
TTTDSTNNNNNNATVVIGKGKPPKEPKPVKPPKEPKPPKEPKP---

---XSYYAVYDGHGGTETSTLLEPTVHNCLVNSQSFRDGDYEQAFRDAYAEADDIVIEKC
EKSGSTGVSALLVGNKLYTANVGDSEIVLARAQPNANPKGPVTYEPVLLSYKHLASDDQE
KKRVTDLGGMIIFNRLFGSLAVSRSFGDKEYKEGEKKFCVSDPYQTTTDLTARDHFFILA
CDGXLG*gri**srsicskkykigkkcn*nfriissrxl
Translated Amino Acid sequence (All Frames) 
Frame A:
GFTTPYNQSNSSPVSSIGSNSSRNSRLPNTSVSIPGLYSSDNDNTRLKNVNNNNNNNNNT
TTYGSSTFPSSVISTTGSISNSIASAMDNDSSYSNESSPTSSMTLLPTTTTTTTTTTTTA
TTTDSTNNNNNNATVVIGKGKPPKEPKPVKPPKEPKPPKEPKP---

---xshtmqfmmvmvvlkpqhswsqqfiivwsilkvsemvtmnklsvmhtlklmil*lkn
vkrvvaqvyrlywleinstlqm*aiqr*f*hvlnqmqilkdpspmnrfysptniwqvmtk
krrespiwvv*sfsivclvhwllvdrlvirstkrvkrsfvslilikqqpisllviissfw
lvmvlwdkveydeavqfvqrniklgksateisellaqdxl

Frame B:
vlqhliinqihhqfhqlvqihlgivvyqilqfqyldyivvimiild*km*iiiiittiiq
qhmdhqhsqvqlyqqlvv*viqlqvqwimivvivmnhhqllq*hyyqllllqqqqplpqq
qqqtlliiitimqr**lekenhqknqnl*nhlknqnhqknqn---

---xlilcsl*wswwy*nlntlganss*lfgqfskfprw*l*tsfp*cir*s**ycn*km
*kew*hrcigsigwk*tlhckcrrfrdsfstcstkcks*rtrhl*tgftllqtfgk**pr
keeshrfgwydhfqsfvwfigc**ivw**gvqrg*kevlcl*slsnnnrshcs*sflhfg
l*wxfgirsnmmkpfnlfkei*nwekvqlkfqny*lkixy

Frame C:
fyntl*sikfitsfinwfkfi*e*sftkyfsfntwii******ysikkck*****qq*yn
niwiinipkfsyinnw*yk*fnckcng****l***iitnffndiitnyyyynnnnhyhsn
nnrly****q*cngsnwkrkttkrtktcktt*rtkttkrtkt---

---XSYYAVYDGHGGTETSTLLEPTVHNCLVNSQSFRDGDYEQAFRDAYAEADDIVIEKC
EKSGSTGVSALLVGNKLYTANVGDSEIVLARAQPNANPKGPVTYEPVLLSYKHLASDDQE
KKRVTDLGGMIIFNRLFGSLAVSRSFGDKEYKEGEKKFCVSDPYQTTTDLTARDHFFILA
CDGXLG*gri**srsicskkykigkkcn*nfriissrxl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA158 (AFA158Q) /CSM/AF/AFA1-C/AFA158Q.Seq.d/                       1275   0.0
SFG594 (SFG594Q) /CSM/SF/SFG5-D/SFG594Q.Seq.d/                       1259   0.0
CHR482 (CHR482Q) /CSM/CH/CHR4-D/CHR482Q.Seq.d/                       1259   0.0
AFE528 (AFE528Q) /CSM/AF/AFE5-B/AFE528Q.Seq.d/                       1259   0.0
CHE537 (CHE537Q) /CSM/CH/CHE5-B/CHE537Q.Seq.d/                       1245   0.0
AFM227 (AFM227Q) /CSM/AF/AFM2-B/AFM227Q.Seq.d/                       1245   0.0
CHN126 (CHN126Q) /CSM/CH/CHN1-B/CHN126Q.Seq.d/                       1154   0.0
AHK426 (AHK426Q) /CSM/AH/AHK4-B/AHK426Q.Seq.d/                       1146   0.0
SLE231 (SLE231Q) /CSM/SL/SLE2-B/SLE231Q.Seq.d/                        940   0.0
SHD445 (SHD445Q) /CSM/SH/SHD4-B/SHD445Q.Seq.d/                        751   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF019985|AF019985.1 Dictyostelium discoideum Spalten (spnA) mRNA, complete cds.  1068  0.0  5
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1068  0.0  5
AF085352|AF085352.1 Archegozetes longisetosus Hox 3 gene, partial cds.    38  0.043  2
M15085|M15085.1 T.brucei expressed copy of the ILTat 1.3 variable surface glycoprotein gene, 5' flank.    38  0.060  2
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    46  0.14  3
AL439327|AL439327.1 T7 end of clone BD0AA003H04 of library BD0AA from strain CBS 94 of  Candida tropicalis.    42  0.14  2
BX511101|BX511101.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    40  0.20  8
AL840627|AL840627.7 Zebrafish DNA sequence from clone CH211-278B16 in linkage group 17.    42  0.24  4
AC131510|AC131510.1 Strongylocentrotus purpuratus clone Sp79A2, *** SEQUENCING IN PROGRESS ***, 5 ordered pieces.    38  0.25  5
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36  0.27  6
dna update 2003. 7.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY328084_1(AY328084|pid:none) Hypocrea jecorina putative serine/...   116   1e-24
(Q09173) RecName: Full=Protein phosphatase 2C homolog 3;        ...   107   2e-23
CU640366_29(CU640366|pid:none) Podospora anserina genomic DNA ch...   112   2e-23
BX294012_38(BX294012|pid:none) Neurospora crassa DNA linkage gro...   110   9e-23
AM920431_242(AM920431|pid:none) Penicillium chrysogenum Wisconsi...   109   9e-23
L34882_1(L34882|pid:none) Schizosaccharomyces pombe protein phos...   105   9e-23
(Q09172) RecName: Full=Protein phosphatase 2C homolog 2;        ...   107   3e-22
CR382131_607(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   101   5e-21
CT005271_264(CT005271|pid:none) Leishmania major strain Friedlin...    97   5e-21
AM502252_211(AM502252|pid:none) Leishmania infantum chromosome 34.     97   6e-21
protein update 2009. 3.25
PSORT 

 psg: 0.85  gvh: 0.46  alm: 0.39  top: 0.60  tms: 0.00  mit: 0.47  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for AFA158 is mit
5' end seq. ID AFA158F
5' end seq. 
>AFA158F.Seq
GGTTTTACAACACCTTATAATCAATCAAATTCATCACCAGTTTCATCAATTGGTTCAAAT
TCATCTAGGAATAGTCGTTTACCAAATACTTCAGTTTCAATACCTGGATTATATAGTAGT
GATAATGATAATACTCGATTAAAAAATGTAAATAATAATAATAATAACAACAATAATACA
ACAACATATGGATCATCAACATTCCCAAGTTCAGTTATATCAACAACTGGTAGTATAAGT
AATTCAATTGCAAGTGCAATGGATAATGATAGTAGTTATAGTAATGAATCATCACCAACT
TCTTCAATGACATTATTACCAACTACTACTACTACAACAACAACAACCACTACCACAGCA
ACAACAACAGACTCTACTAATAATAATAACAATAATGCAACGGTAGTAATTGGAAAAGGA
AAACCACCAAAAGAACCAAAACCTGTAAAACCACCTAAAGAACCAAAACCACCAAAAGAA
CCAAAACCA----------
Length of 5' end seq. 489
3' end seq. ID AFA158Z
3' end seq. 
>AFA158Z.Seq
----------CANTCTCATACTATGCAGTTTATGATGGTCATGGTGGTACTGAAACCTCA
ACACTCTTGGAGCCAACAGTTCATAATTGTTTGGTCAATTCTCAAAGTTTCCGAGATGGT
GACTATGAACAAGCTTTCCGTGATGCATACGCTGAAGCTGATGATATTGTAATTGAAAAA
TGTGAAAAGAGTGGTAGCACAGGTGTATCGGCTCTATTGGTTGGAAATAAACTCTACACT
GCAAATGTAGGCGATTCAGAGATAGTTTTAGCACGTGCTCAACCAAATGCAAATCCTAAA
GGACCCGTCACCTATGAACCGGTTTTACTCTCCTACAAACATTTGGCAAGTGATGACCAA
GAAAAGAAGAGAGTCACCGATTTGGGTGGTATGATCATTTTCAATCGTTTGTTTGGTTCA
TTGGCTGTTAGTAGATCGTTTGGTGATAAGGAGTACAAAGAGGGTGAAAAGAAGTTTTGT
GTCTCTGATCCTTATCAAACAACAACCGATCTCACTGCTCGTGATCATTTCTTCATTTTG
GCTTGTGATGGTNCTTTGGGATAAGGTCGAATATGATGAAGCCGTTCAATTTGTTCAAAG
AAATATAAAATTGGGAAAAAGTGCAACTGAAATTTCAGAATTATTAGCTCAAGATTNCTT
AT
Length of 3' end seq. 652
Connected seq. ID AFA158P
Connected seq. 
>AFA158P.Seq
GGTTTTACAACACCTTATAATCAATCAAATTCATCACCAGTTTCATCAATTGGTTCAAAT
TCATCTAGGAATAGTCGTTTACCAAATACTTCAGTTTCAATACCTGGATTATATAGTAGT
GATAATGATAATACTCGATTAAAAAATGTAAATAATAATAATAATAACAACAATAATACA
ACAACATATGGATCATCAACATTCCCAAGTTCAGTTATATCAACAACTGGTAGTATAAGT
AATTCAATTGCAAGTGCAATGGATAATGATAGTAGTTATAGTAATGAATCATCACCAACT
TCTTCAATGACATTATTACCAACTACTACTACTACAACAACAACAACCACTACCACAGCA
ACAACAACAGACTCTACTAATAATAATAACAATAATGCAACGGTAGTAATTGGAAAAGGA
AAACCACCAAAAGAACCAAAACCTGTAAAACCACCTAAAGAACCAAAACCACCAAAAGAA
CCAAAACCA----------CANTCTCATACTATGCAGTTTATGATGGTCATGGTGGTACT
GAAACCTCAACACTCTTGGAGCCAACAGTTCATAATTGTTTGGTCAATTCTCAAAGTTTC
CGAGATGGTGACTATGAACAAGCTTTCCGTGATGCATACGCTGAAGCTGATGATATTGTA
ATTGAAAAATGTGAAAAGAGTGGTAGCACAGGTGTATCGGCTCTATTGGTTGGAAATAAA
CTCTACACTGCAAATGTAGGCGATTCAGAGATAGTTTTAGCACGTGCTCAACCAAATGCA
AATCCTAAAGGACCCGTCACCTATGAACCGGTTTTACTCTCCTACAAACATTTGGCAAGT
GATGACCAAGAAAAGAAGAGAGTCACCGATTTGGGTGGTATGATCATTTTCAATCGTTTG
TTTGGTTCATTGGCTGTTAGTAGATCGTTTGGTGATAAGGAGTACAAAGAGGGTGAAAAG
AAGTTTTGTGTCTCTGATCCTTATCAAACAACAACCGATCTCACTGCTCGTGATCATTTC
TTCATTTTGGCTTGTGATGGTNCTTTGGGATAAGGTCGAATATGATGAAGCCGTTCAATT
TGTTCAAAGAAATATAAAATTGGGAAAAAGTGCAACTGAAATTTCAGAATTATTAGCTCA
AGATTNCTTAT
Length of connected seq. 1141
Full length Seq ID -
Full length Seq. -
Length of full length seq. -