>FC-AO13 (FC-AO13Q) /CSM/FC/FC-AO/FC-AO13Q.Seq.d/
XXXXXXXXXXACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTT
CAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCACTTTGAGTTTTCAAATATAGTAATCAAATCTAAAAAAAATTTTAAAAAA
AAAATAATAAAAAAAAAAAAATTAAAAAAAATAAAAAAAAAAAAAAAAA

---LNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWAS
IGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfkysnqi*kkf*kk
nnkkkkikknkkkkk

Frame A:
---LNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWAS
IGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfkysnqi*kkf*kk
nnkkkkikknkkkkk


Frame B:
---sivvevlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglq
lvlstkirkplksl*pperekv*pqksqiltnstknskqfkshfefsnivikskknfkkk
iikkkklkkikkkkk


Frame C:
---qss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrslgfn
wccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtlsfqi**snlkkilkkk
**kkkn*kk*kkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSB147 (VSB147Q) /CSM/VS/VSB1-B/VSB147Q.Seq.d/                        658   0.0
VHG546 (VHG546Q) /CSM/VH/VHG5-B/VHG546Q.Seq.d/                        658   0.0
VHP749 (VHP749Q) /CSM/VH/VHP7-C/VHP749Q.Seq.d/                        652   0.0
VHO803 (VHO803Q) /CSM/VH/VHO8-A/VHO803Q.Seq.d/                        652   0.0
VHO504 (VHO504Q) /CSM/VH/VHO5-A/VHO504Q.Seq.d/                        652   0.0
VHO104 (VHO104Q) /CSM/VH/VHO1-A/VHO104Q.Seq.d/                        652   0.0
VHI806 (VHI806Q) /CSM/VH/VHI8-A/VHI806Q.Seq.d/                        652   0.0
VHD305 (VHD305Q) /CSM/VH/VHD3-A/VHD305Q.Seq.d/                        652   0.0
VHD106 (VHD106Q) /CSM/VH/VHD1-A/VHD106Q.Seq.d/                        652   0.0
VHB715 (VHB715Q) /CSM/VH/VHB7-A/VHB715Q.Seq.d/                        652   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   504  e-161  2
BM395467|BM395467.1 50072-2-9-C10.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    60  3e-12  3
AF534908|AF534908.1 Tetrahymena thermophila elongation factor 2 gene, complete cds.    60  3e-11  3
BM058379|BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence.    76  4e-10  1
BM058578|BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, mRNA sequence.    76  4e-10  1
BM056853|BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA sequence.    70  2e-08  1
AL111223|AL111223.1 Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation.    68  9e-08  1
BM059287|BM059287.1 2241-52 hindgut and Malpighian tubule cDNA library Ctenocephalides felis cDNA clone 2241-52, mRNA sequence.    48  2e-07  2
AJ299248|AJ299248.1 Nicotiana tabacum partial mRNA for elongation factor 2 (ef2 gene),  clone 3cT7.    66  3e-07  1
BU495517|BU495517.1 PfESToab73h09.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q61509 Q61509 EUKARYOTIC TRANSLATION ELONGATION FACTOR 2 ;, mRNA sequence.    56  3e-06  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q06193) RecName: Full=Elongation factor 2;          Short=EF-2;...   103   2e-21
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...    96   5e-19
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...    95   6e-19
CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...    95   6e-19
AF544973_1(AF544973|pid:none) Ctenopharyngodon idella elongation...    94   1e-18
(P09445) RecName: Full=Elongation factor 2;          Short=EF-2;...    94   1e-18
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...    94   1e-18
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...    94   1e-18
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...    94   1e-18
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...    94   1e-18

 psg: 0.49  gvh: 0.48  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.02
 nuc: 0.04  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for FC-AO13 is nuc

>FC-AO13Z.Seq
----------ACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTT
CAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCACTTTGAGTTTTCAAATATAGTAATCAAATCTAAAAAAAATTTTAAAAAA
AAAATAATAAAAAAAAAAAAATTAAAAAAAATAAAAAAAAAAAAAAAAA