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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH647
Library AF
(Link to library)
Clone ID AFH647
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFH647Z
(Link to Original site)
Representative DNA sequence 
>AFH647 (AFH647Q) /CSM/AF/AFH6-B/AFH647Q.Seq.d/
XXXXXXXXXXGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTG
GTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCG
ATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTG
GTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTA
AGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGG
TTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAAT
TAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAA
ATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTG
ATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTG
AATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATA
ATGTTGAATCTAAAAAAGATTCTTCTGCTAAACATGATAATTATAAATAAATTTAATAAA
TATA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
---VDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGG
KVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKL
GEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVE
FIQDNAAIEFKLPSSQTDDNVESKKDSSAKHDNYK*i**i
Translated Amino Acid sequence (All Frames) 
Frame A:
---VDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGG
KVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKL
GEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVE
FIQDNAAIEFKLPSSQTDDNVESKKDSSAKHDNYK*i**i


Frame B:
---si*rnshnkqliwvslvklfqpslliqlpiklvtismkkkpshsipspngskmslvv
kflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*
vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tlln
sfkitlplnsnyhhlklmimlnlkkilllnmiiinkfnky


Frame C:
---ryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*
sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir
*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*i
hsr*rch*iqitiisn***c*i*krffc*t**l*inlini
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO829 (AFO829Q) /CSM/AF/AFO8-B/AFO829Q.Seq.d/                       1296   0.0
AFM402 (AFM402Q) /CSM/AF/AFM4-A/AFM402Q.Seq.d/                       1296   0.0
AFL565 (AFL565Q) /CSM/AF/AFL5-C/AFL565Q.Seq.d/                       1296   0.0
AFH647 (AFH647Q) /CSM/AF/AFH6-B/AFH647Q.Seq.d/                       1296   0.0
AFI356 (AFI356Q) /CSM/AF/AFI3-C/AFI356Q.Seq.d/                       1292   0.0
AHO764 (AHO764Q) /CSM/AH/AHO7-C/AHO764Q.Seq.d/                       1291   0.0
AFL789 (AFL789Q) /CSM/AF/AFL7-D/AFL789Q.Seq.d/                       1291   0.0
AFK581 (AFK581Q) /CSM/AF/AFK5-D/AFK581Q.Seq.d/                       1291   0.0
AFN839 (AFN839Q) /CSM/AF/AFN8-B/AFN839Q.Seq.d/                       1283   0.0
VFM432 (VFM432Q) /CSM/VF/VFM4-B/VFM432Q.Seq.d/                       1275   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BC067155|BC067155.1 Danio rerio hypothetical protein zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   5e-08  1
CK029548|CK029548.1 AGENCOURT_16620510 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7051751 5', mRNA sequence.    70   5e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   5e-08  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
dna update 2004. 5.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   168   1e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   166   4e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   162   1e-38
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   161   1e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   157   3e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   152   6e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   1e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   149   5e-35
protein update 2009. 4.21
PSORT 

 psg: 0.84  gvh: 0.38  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFH647 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFH647Z
3' end seq. 
>AFH647Z.Seq
----------GGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTG
GTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCG
ATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTG
GTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTA
AGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGG
TTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAAT
TAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAA
ATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTG
ATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTG
AATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATA
ATGTTGAATCTAAAAAAGATTCTTCTGCTAAACATGATAATTATAAATAAATTTAATAAA
TATA
Length of 3' end seq. 654
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -