>FC-AB09 (FC-AB09Q) /CSM/FC/FC-AB/FC-AB09Q.Seq.d/
GATGAAAGTGCTTCTCACAAACAATTATGTAAAATATATAATAAAATACATTATTTAATC
ATTTTTATTAATTTTGTTTTAGTTGTTGATCTTTATCCGACTATTTAAAATTAATTGTAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAXXXXXXXXXXAAAAGA
AAAAAAAAAAAAAAAGGAAAAAAACAAAACCAAAAGAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAA

mkvlltnnyvkyiikyii*sfllilf*LLIFIRLFKINCKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKK---

---KRKKKKKGKKQNQKKKKKKKKKKKKKKKKKK

Frame A:
desashkqlckiynkihyliifinfvlvvdlypti*n*l*kkkkkkkkkkkkkkkkkkkk
kkkkkkkkkkkkkk---

---KRKKKKKGKKQNQKKKKKKKKKKKKKKKKKK

Frame B:
mkvlltnnyvkyiikyii*sfllilf*LLIFIRLFKINCKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKK---

---kekkkkkeknktkrkkkkkkkkkkkkkkkk

Frame C:
*kcfsqtim*ni**ntlfnhfy*fcfsc*slsdylklivkkkkkkkkkkkkkkkkkkkkk
kkkkkkkkkkkkkk---

---kkkkkkrkktkpkekkkkkkkkkkkkkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE149 (SLE149Q) /CSM/SL/SLE1-C/SLE149Q.Seq.d/                        234   7e-61
SLE101 (SLE101Q) /CSM/SL/SLE1-A/SLE101Q.Seq.d/                        234   7e-61
SLD716 (SLD716Q) /CSM/SL/SLD7-A/SLD716Q.Seq.d/                        234   7e-61
SLA720 (SLA720Q) /CSM/SL/SLA7-A/SLA720Q.Seq.d/                        234   7e-61
FC-IC0269 (FC-IC0269Q) /CSM/FC-IC/FC-IC0269Q.Seq.d/                   234   7e-61
FC-AB09 (FC-AB09Q) /CSM/FC/FC-AB/FC-AB09Q.Seq.d/                      234   7e-61
SLG796 (SLG796Q) /CSM/SL/SLG7-D/SLG796Q.Seq.d/                        222   3e-57
SLB790 (SLB790Q) /CSM/SL/SLB7-D/SLB790Q.Seq.d/                        222   3e-57
CHE469 (CHE469Q) /CSM/CH/CHE4-C/CHE469Q.Seq.d/                        178   3e-44
VFE354 (VFE354Q) /CSM/VF/VFE3-C/VFE354Q.Seq.d/                        165   5e-40

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   234  5e-58  6
K02961|K02961.1 Slime mold (D.discoideum) actin mRNA ITL-1, 3' end.   137  3e-50  2
M25218|M25218.1 Dictyostelium discoideum actin ITL-1 gene, 3' UTR.   137  7e-46  2
M25217|M25217.1 Dictyostelium discoideum actin 111-12/A1 gene, 3' UTR.    40  2e-04  3
M25221|M25221.1 Dictyostelium discoideum actin 2-1 gene, 3' UTR.    38  7e-04  2
BE117827|BE117827.1 UI-R-BS1-azh-e-02-0-UI.s1 UI-R-BS1 Rattus norvegicus cDNA clone UI-R-BS1-azh-e-02-0-UI 3', mRNA sequence.    38  9e-04  2
AW521543|AW521543.1 UI-R-BO0-agn-g-11-0-UI.s1 UI-R-BO0 Rattus norvegicus cDNA clone UI-R-BO0-agn-g-11-0-UI 3', mRNA sequence.    38  9e-04  2
AA955176|AA955176.1 UI-R-A1-du-b-05-0-UI.s1 UI-R-A1 Rattus norvegicus cDNA clone UI-R-A1-du-b-05-0-UI 3', mRNA sequence.    38  0.001  2
K02954|K02954.1 Slime mold (D.discoideum) actin 2-sub 1 gene, 3' end.    38  0.001  2
J01278|J01278.1 Slime mold (D.discoideum) actin mRNA, coding region 3' end.    40  0.001  3

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 104,162,074

 psg: 0.30  gvh: 0.23  alm: 0.59  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.00
 nuc: 7.08  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for FC-AB09 is nuc

>FC-AB09F.Seq
GATGAAAGTGCTTCTCACAAACAATTATGTAAAATATATAATAAAATACATTATTTAATC
ATTTTTATTAATTTTGTTTTAGTTGTTGATCTTTATCCGACTATTTAAAATTAATTGTAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA----------