>FC-AA22 (FC-AA22Q) /CSM/FC/FC-AA/FC-AA22Q.Seq.d/
ATGAATACGATGATAGTGATTCAGACTTTTGACCAATTGAAAAAACCAGCAACAGAAATG
GTACTGGTTTGGTCTCCTCCACTTTTAAGGTTGCCCCTTCCTTCTCTACCATTCAAAAAC
AACAACAACAACAACAAAAATCACTTTCACCAAAAACAACATTAACAACAACTATTACAA
CAACTACAACAACAACAACAATAATTAAAGAAAAATACAAAAATGAAAAAAATACAACTA
CAACTACATCTAATACAAACAAACCATTACAAATCAATTCCATTAATAACAAAAACGGTT
TAATCAATGAATCATCATCATCATCATCAACTCTTTCAACACTTGAAACAAATGTAAATT
CCAGTGAATTATTTGTATCGTGTGAAAAGTTGTCCAACAACAATTATCATCATCAAAATG
AAGATGATATTGTTGTTTCATTGATTCAAACTGAAAGAGTAAACATACAAAAGAAGTTAG
GGGCTTAAACCATTCTCTAAAAATAAATTAAATAAAAAAAATGGCTTTGTTTTGTCGTTA
GTTGTCACTTAAAAAAATAAAACAAAAAAAAAAAAA

eyddsdsdf*PIEKTSNRNGTGLVSSTFKVAPSFSTIQKQQQQQQKSLSPKTTLTTTITT
TTTTTTIIKEKYKNEKNTTTTTSNTNKPLQINSINNKNGLINESSSSSSTLSTLETNVNS
SELFVSCEKLSNNNYHHQNEDDIVVSLIQTERVNIQKKLGA*til*k*ik*kkwlcfvvs
chlkk*nkkkk

Frame A:
mntmiviqtfdqlkkpatemvlvwsppllrlplpslpfknnnnnnknhfhqkqh*qqllq
qlqqqqq*lkkntkmkkiqlqlhliqtnhyksiplitktv*smnhhhhhqlfqhlkqm*i
pvnylyrvkscpttiiiikmkmillfh*fklke*tykrs*glkpfsknklnkkngfvlsl
vvt*knktkkkk


Frame B:
*ir***frlltn*knqqqkwywfgllhf*gcpfllyhskttttttkitftknninnnyyn
nynnnnnn*rkiqk*kkynynyi*ykqtitnqfh**qkrfnq*iiiiiinsfnt*nkckf
q*iiciv*kvvqqqlsssk*r*yccfidsn*kskhtkevrglnhslkin*ikkmalfcr*
lslkkikqkkk


Frame C:
eyddsdsdf*PIEKTSNRNGTGLVSSTFKVAPSFSTIQKQQQQQQKSLSPKTTLTTTITT
TTTTTTIIKEKYKNEKNTTTTTSNTNKPLQINSINNKNGLINESSSSSSTLSTLETNVNS
SELFVSCEKLSNNNYHHQNEDDIVVSLIQTERVNIQKKLGA*til*k*ik*kkwlcfvvs
chlkk*nkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSB221 (VSB221Q) /CSM/VS/VSB2-A/VSB221Q.Seq.d/                        333   2e-90
VFM750 (VFM750Q) /CSM/VF/VFM7-C/VFM750Q.Seq.d/                        333   2e-90
FC-AA22 (FC-AA22Q) /CSM/FC/FC-AA/FC-AA22Q.Seq.d/                      333   2e-90
AFK883 (AFK883Q) /CSM/AF/AFK8-D/AFK883Q.Seq.d/                        333   2e-90
AFG187 (AFG187Q) /CSM/AF/AFG1-D/AFG187Q.Seq.d/                        333   2e-90
VFK565 (VFK565Q) /CSM/VF/VFK5-C/VFK565Q.Seq.d/                        327   1e-88
CFH851 (CFH851Q) /CSM/CF/CFH8-C/CFH851Q.Seq.d/                        317   1e-85
AHN667 (AHN667Q) /CSM/AH/AHN6-C/AHN667Q.Seq.d/                        297   1e-79
VFJ767 (VFJ767Q) /CSM/VF/VFJ7-C/VFJ767Q.Seq.d/                        295   4e-79
AFB636 (AFB636Q) /CSM/AF/AFB6-B/AFB636Q.Seq.d/                        295   4e-79

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF298834|AF298834.1 Dictyostelium discoideum eukaryotic release factor 1 (erf1) mRNA, complete cds.   220   2e-53  1
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    34   0.11   10
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    34   0.26   8
AL109622|AL109622.10 Human DNA sequence from clone RP3-526F5 on chromosome Xq26.3-28.  Contains the gene for a novel protein similar to UBE2N  (ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)),  ESTs, STSs and GSSs.    46   0.52   1
BP112853|BP112853.1 Bos taurus cDNA, clone:ORCS13670, 5' end.      46   0.52   1
AC116979|AC116979.2 Dictyostelium discoideum chromosome 2 map 6357117-6445670 strain AX4, complete sequence.    42   1.1    4
AC009685|AC009685.3 Homo sapiens chromosome 15 clone RP11-91E13 map 15, *** SEQUENCING IN PROGRESS ***, 31 unordered pieces.    40   1.4    6
AC124155|AC124155.2 Danio rerio clone CH211-46C3 strain Tue, WORKING DRAFT SEQUENCE, 6 ordered pieces.    44   2.0    1
BX663512|BX663512.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-213F9.    44   2.0    1
AC016079|AC016079.6 Homo sapiens chromosome 15 clone RP11-23M19 map 15, WORKING DRAFT SEQUENCE, 8 unordered pieces.    44   2.0    1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 586,713,196

 psg: 0.90  gvh: 0.51  alm: 0.48  top: 0.60  tms: 0.00  mit: 0.59  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for FC-AA22 is mit

>FC-AA22E.Seq
ATGAATACGATGATAGTGATTCAGACTTTTGACCAATTGAAAAAACCAGCAACAGAAATG
GTACTGGTTTGGTCTCCTCCACTTTTAAGGTTGCCCCTTCCTTCTCTACCATTCAAAAAC
AACAACAACAACAACAAAAATCACTTTCACCAAAAACAACATTAACAACAACTATTACAA
CAACTACAACAACAACAACAATAATTAAAGAAAAATACAAAAATGAAAAAAATACAACTA
CAACTACATCTAATACAAACAAACCATTACAAATCAATTCCATTAATAACAAAAACGGTT
TAATCAATGAATCATCATCATCATCATCAACTCTTTCAACACTTGAAACAAATGTAAATT
CCAGTGAATTATTTGTATCGTGTGAAAAGTTGTCCAACAACAATTATCATCATCAAAATG
AAGATGATATTGTTGTTTCATTGATTCAAACTGAAAGAGTAAACATACAAAAGAAGTTAG
GGGCTTAAACCATTCTCTAAAAATAAATTAAATAAAAAAAATGGCTTTGTTTTGTCGTTA
GTTGTCACTTAAAAAAATAAAACAAAAAAAAAAAAA