NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
FC-AA03
Library FC
(Link to library)
Clone ID FC-AA03
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15331-1
Original site URL
Representative seq. ID FC-AA03P
(Link to Original site)
Representative DNA sequence 
>FC-AA03 (FC-AA03Q) /CSM/FC/FC-AA/FC-AA03Q.Seq.d/
AAAATGTCATCTTATTTATTCACTAGTGAATCCGTCACCGAAGTCATCCAGATAAAATCT
GTGATCAAGTATCAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAG
TTGCTTGTGAAACTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCA
AAGCCAACGTTGACTATCAAAAAGTTGTTCGTGAAGCTGTTAAAAAGATTGGTTATGATG
ACTCATCAAAAGGTTTTGATTACAAAACTTGCAATGTTTTAGTTGCAATTGAACAACAAT
CACCAGATATTGCTCAAGGTGTCCACATTGGTAAAGTTAACCCAGAAGATATTGGTGCTG
GTGATCAAGGTCACATGTTTGGTTATGCCACCAACGAAACCCCAACTTTAATGCCACTTA
CTCATTTCCTCGCCTCTGAATTAGTCAACAAATTAACTGAATTACGTCACAATGGTACCT
TAGCCTGGGCTCGTCCAGATGCCAAGACTCAAGTCACTGTTGAATACAAAAAAGAAGGTC
ACAAAATCACTCCAGTCAGAGTACACACCATCGTTATTTCAACTCAACACGACGAXXXXX
XXXXXCCGGTCGTAAGATCATCATTGATTCATATGGTGGTTGGGGTGCTCACGGTGGTGG
TGCTTTCTCTGGTAAAGATGCCACTAAAGTCGATCGTTCTGGTGCTTACGCTGCTAGATG
GATCGCTAAATCTTTAGTTGCCGCTGGTCTTGCCGATCGTTGTTTAGTTCAAGGTTCATA
CTCTATTGGTGTTGCCAAGCCACTCAGTGTCTTTGTTGATACCTATGGTACCGGTAAAAA
GACTGATGCTGAAATCCTTGCCATCATCGATAATAACTTTGATTTACGTCCAGGTTGTTT
AATGCGTGACCTCCAATTAACTCGTCCAATTCTCCAAAAAACTGCCTCTGGTGGTCACTT
TGGTAGAAATGATCCAGACTTTACCTGGGAAACCGTCAAAGAACTCAAGATGTAAGGATA
GATCAATGAATAAATAAGATAGATAAATAAGTGAAAAAAAAAAAATATAATAATCAAAAA
AAAAAAAAAAAAAAAAAAAACTAAAACGCAGTTTAATAAAAAAATTTAAACATTTTAAAA
TAAAAAAAAAA
sequence update 1996.12.12
Translated Amino Acid sequence 
nvilfih**IRHRSHPDKICDQVSDAVLDACLAQDPLSKVACETATKTGMVMILGEITTK
ANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGVHIGKVNPEDIGAG
DQGHMFGYATNETPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAKTQVTVEYKKEGH
KITPVRVHTIVISTQHD---

---GRKIIIDSYGGWGAHGGGAFSGKDATKVDRSGAYAARWIAKSLVAAGLADRCLVQGS
YSIGVAKPLSVFVDTYGTGKKTDAEILAIIDNNFDLRPGCLMRDLQLTRPILQKTASGGH
FGRNDPDFTWETVKELKM*g*ine*ir*ink*kkkniiikkkkkkkktktqfnkki*tf*
nkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
kmssylftsesvteviqiksvikyqmlfsmlv*lkihyqkllvklqpklvwl*f*vklpp
kptltikklfvkllkrlvmmthqkvlitklamf*lqlnnnhqillkvstlvkltqkilvl
vikvtclvmpptkpql*chllisspln*stn*lnyvtmvp*pglvqmprlksllntkkkv
tkslqseytpslfqlntt---

---pvvrsslihmvvgvltvvvlslvkmplksivlvltlldgslnl*lplvlpivv*fkv
htllvlpshsvsllipmvpvkrlmlkslpssiitliyvqvv*cvtsn*lvqfskklplvv
tlvemiqtlpgkpsknsrckdrsmnk*dr*isekkki**skkkkkkkklkrslikkfkhf
kikkk

Frame B:
kchliyslvnpspkssr*nl*ssirccsrclfssrsiikscl*ncnqnwygydfr*nyhq
sqr*lskscs*sc*kdwl**likrf*lqnlqcfscn*ttitrycsrcphw*s*prrywcw
*srshvwlchqrnpnfnatysfprl*isqqin*itsqwylslgssrcqdsshc*iqkrrs
qnhssqsthhryfnstrr---

---rs*dhh*fiwwlgcsrwwcflw*rch*srsfwclrc*mdr*ifscrwscrslfssrf
ilywccqatqclc*ylwyr*kd*c*npchhr**l*ftsrlfna*ppinssnspknclwws
lw*k*srlylgnrqrtqdvridq*inkidk*vkkkkynnqkkkkkkkn*nav**knlnil
k*kk

Frame C:
nvilfih**IRHRSHPDKICDQVSDAVLDACLAQDPLSKVACETATKTGMVMILGEITTK
ANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGVHIGKVNPEDIGAG
DQGHMFGYATNETPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAKTQVTVEYKKEGH
KITPVRVHTIVISTQHD---

---GRKIIIDSYGGWGAHGGGAFSGKDATKVDRSGAYAARWIAKSLVAAGLADRCLVQGS
YSIGVAKPLSVFVDTYGTGKKTDAEILAIIDNNFDLRPGCLMRDLQLTRPILQKTASGGH
FGRNDPDFTWETVKELKM*g*ine*ir*ink*kkkniiikkkkkkkktktqfnkki*tf*
nkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FC-AA03 (FC-AA03Q) /CSM/FC/FC-AA/FC-AA03Q.Seq.d/                     2028   0.0
SLA104 (SLA104Q) /CSM/SL/SLA1-A/SLA104Q.Seq.d/                       1179   0.0
FC-003 (FC-003Q) /CSM/CG/CG-FC/FC-003Q.Seq.d/                        1179   0.0
VFN312 (VFN312Q) /CSM/VF/VFN3-A/VFN312Q.Seq.d/                       1166   0.0
VFN128 (VFN128Q) /CSM/VF/VFN1-B/VFN128Q.Seq.d/                       1166   0.0
VFM886 (VFM886Q) /CSM/VF/VFM8-D/VFM886Q.Seq.d/                       1166   0.0
VFM766 (VFM766Q) /CSM/VF/VFM7-C/VFM766Q.Seq.d/                       1166   0.0
VFL503 (VFL503Q) /CSM/VF/VFL5-A/VFL503Q.Seq.d/                       1166   0.0
VFL319 (VFL319Q) /CSM/VF/VFL3-A/VFL319Q.Seq.d/                       1166   0.0
VFL166 (VFL166Q) /CSM/VF/VFL1-C/VFL166Q.Seq.d/                       1166   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ390497|AJ390497.1 Candida albicans partial mRNA for S-adenosylmethionine synthetase 2  (sam2 gene).   107  8e-59  4
AX595308|AX595308.1 Sequence 962 from Patent EP1258494.          100  1e-51  6
M23368|M23368.1 Saccharomyces cerevisiae S-adenosylmethionine synthetase (SAM2) gene, complete cds.   100  1e-50  6
U33057|U33057.1 Saccharomyces cerevisiae chromosome IV cosmids 8166, 9787, 9717, and lambda 3073.   100  4e-43  6
AL424144|AL424144.1 T7 end of clone AZ0AA012F03 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    78  3e-33  5
AJ001705|AJ001705.1 Schizosaccharomyces pombe sam1 gene.          86  5e-33  6
Z71271|Z71271.1 C.roseus mRNA for S-adenosyl-L-methionine synthetase 1.    80  8e-33  4
BG589726|BG589726.1 EST497568 P. infestans-challenged leaf Solanum tuberosum cDNA clone BPLI2F2 5' sequence, mRNA sequence.    92  9e-31  3
BG350671|BG350671.1 096C01 Mature tuber lambda ZAP Solanum tuberosum cDNA, mRNA sequence.    92  1e-30  3
BG593481|BG593481.1 EST492159 cSTS Solanum tuberosum cDNA clone cSTS4F17 5' sequence, mRNA sequence.    90  3e-30  3
dna update 2003. 7.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54F07) RecName: Full=S-adenosylmethionine synthetase;         ...   374   e-104
DQ087477_1(DQ087477|pid:none) Microciona prolifera methionine ad...   241   9e-87
(Q0BAY8) RecName: Full=S-adenosylmethionine synthetase;         ...   200   4e-86
(A0KBF2) RecName: Full=S-adenosylmethionine synthetase;         ...   200   5e-86
AM747720_514(AM747720|pid:none) Burkholderia cenocepacia J2315 c...   200   5e-86
CP000868_3067(CP000868|pid:none) Burkholderia multivorans ATCC 1...   199   7e-86
AP009385_151(AP009385|pid:none) Burkholderia multivorans ATCC 17...   199   7e-86
(Q2T267) RecName: Full=S-adenosylmethionine synthetase;         ...   201   9e-86
(A4JIQ4) RecName: Full=S-adenosylmethionine synthetase;         ...   199   1e-85
(A7NFR6) RecName: Full=S-adenosylmethionine synthetase;         ...   194   4e-85
protein update 2009. 4.13
PSORT 

 psg: 0.62  gvh: 0.43  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  4.0 %: peroxisomal

>> prediction for FC-AA03 is nuc
5' end seq. ID FC-AA03F
5' end seq. 
>FC-AA03F.Seq
AAAATGTCATCTTATTTATTCACTAGTGAATCCGTCACCGAAGTCATCCAGATAAAATCT
GTGATCAAGTATCAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAG
TTGCTTGTGAAACTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCA
AAGCCAACGTTGACTATCAAAAAGTTGTTCGTGAAGCTGTTAAAAAGATTGGTTATGATG
ACTCATCAAAAGGTTTTGATTACAAAACTTGCAATGTTTTAGTTGCAATTGAACAACAAT
CACCAGATATTGCTCAAGGTGTCCACATTGGTAAAGTTAACCCAGAAGATATTGGTGCTG
GTGATCAAGGTCACATGTTTGGTTATGCCACCAACGAAACCCCAACTTTAATGCCACTTA
CTCATTTCCTCGCCTCTGAATTAGTCAACAAATTAACTGAATTACGTCACAATGGTACCT
TAGCCTGGGCTCGTCCAGATGCCAAGACTCAAGTCACTGTTGAATACAAAAAAGAAGGTC
ACAAAATCACTCCAGTCAGAGTACACACCATCGTTATTTCAACTCAACACGACGA-----
-----
Length of 5' end seq. 595
3' end seq. ID FC-AA03Z
3' end seq. 
>FC-AA03Z.Seq
----------CCGGTCGTAAGATCATCATTGATTCATATGGTGGTTGGGGTGCTCACGGT
GGTGGTGCTTTCTCTGGTAAAGATGCCACTAAAGTCGATCGTTCTGGTGCTTACGCTGCT
AGATGGATCGCTAAATCTTTAGTTGCCGCTGGTCTTGCCGATCGTTGTTTAGTTCAAGGT
TCATACTCTATTGGTGTTGCCAAGCCACTCAGTGTCTTTGTTGATACCTATGGTACCGGT
AAAAAGACTGATGCTGAAATCCTTGCCATCATCGATAATAACTTTGATTTACGTCCAGGT
TGTTTAATGCGTGACCTCCAATTAACTCGTCCAATTCTCCAAAAAACTGCCTCTGGTGGT
CACTTTGGTAGAAATGATCCAGACTTTACCTGGGAAACCGTCAAAGAACTCAAGATGTAA
GGATAGATCAATGAATAAATAAGATAGATAAATAAGTGAAAAAAAAAAAATATAATAATC
AAAAAAAAAAAAAAAAAAAAAAAAACTAAAACGCAGTTTAATAAAAAAATTTAAACATTT
TAAAATAAAAAAAAAA
Length of 3' end seq. 546
Connected seq. ID FC-AA03P
Connected seq. 
>FC-AA03P.Seq
AAAATGTCATCTTATTTATTCACTAGTGAATCCGTCACCGAAGTCATCCAGATAAAATCT
GTGATCAAGTATCAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAG
TTGCTTGTGAAACTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCA
AAGCCAACGTTGACTATCAAAAAGTTGTTCGTGAAGCTGTTAAAAAGATTGGTTATGATG
ACTCATCAAAAGGTTTTGATTACAAAACTTGCAATGTTTTAGTTGCAATTGAACAACAAT
CACCAGATATTGCTCAAGGTGTCCACATTGGTAAAGTTAACCCAGAAGATATTGGTGCTG
GTGATCAAGGTCACATGTTTGGTTATGCCACCAACGAAACCCCAACTTTAATGCCACTTA
CTCATTTCCTCGCCTCTGAATTAGTCAACAAATTAACTGAATTACGTCACAATGGTACCT
TAGCCTGGGCTCGTCCAGATGCCAAGACTCAAGTCACTGTTGAATACAAAAAAGAAGGTC
ACAAAATCACTCCAGTCAGAGTACACACCATCGTTATTTCAACTCAACACGACGA-----
-----CCGGTCGTAAGATCATCATTGATTCATATGGTGGTTGGGGTGCTCACGGTGGTGG
TGCTTTCTCTGGTAAAGATGCCACTAAAGTCGATCGTTCTGGTGCTTACGCTGCTAGATG
GATCGCTAAATCTTTAGTTGCCGCTGGTCTTGCCGATCGTTGTTTAGTTCAAGGTTCATA
CTCTATTGGTGTTGCCAAGCCACTCAGTGTCTTTGTTGATACCTATGGTACCGGTAAAAA
GACTGATGCTGAAATCCTTGCCATCATCGATAATAACTTTGATTTACGTCCAGGTTGTTT
AATGCGTGACCTCCAATTAACTCGTCCAATTCTCCAAAAAACTGCCTCTGGTGGTCACTT
TGGTAGAAATGATCCAGACTTTACCTGGGAAACCGTCAAAGAACTCAAGATGTAAGGATA
GATCAATGAATAAATAAGATAGATAAATAAGTGAAAAAAAAAAAATATAATAATCAAAAA
AAAAAAAAAAAAAAAAAAAACTAAAACGCAGTTTAATAAAAAAATTTAAACATTTTAAAA
TAAAAAAAAAA
Length of connected seq. 1141
Full length Seq ID -
Full length Seq. -
Length of full length seq. -