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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH636
Library AF
(Link to library)
Clone ID AFH636
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15599-1
Original site URL
Representative seq. ID AFH636P
(Link to Original site)
Representative DNA sequence 
>AFH636 (AFH636Q) /CSM/AF/AFH6-B/AFH636Q.Seq.d/
CACTGTTGGCCTACTGGAGGCAAAAACACCAGGACTTTAAAAAAACACTGCTTTTTTTTT
TTATATTTTTTTATTTTTTTTTATTTTTTTNATTCATATNAATTCTGAATNGGATATAAA
NGTAATNTTNCAAATAAAAAAAAAAXXXXXXXXXXCCTACCTTAATTATTATTTTGGTAG
TGAAAATGATGGTATGTCAGCTATTCAATCATTTTTATCACAACCAGATAATAGCGACCC
ATCAAATTCCGAAGCATTTGTAATTGGTTTTGGTGACCTTGGTACCACATTTCCTTATAC
AGCATTGGTCGAAACTCAATATCCAGCAAGTGAAACAATTGCAGCAATTTCCCAAACCAT
TAGTGCACCATATGGTTCATCACCATTTGTTAGAGCAATGGGTAAACAAAGTAATTCAAT
CGATAGATTAGATCCAAGTCAAACACCATTTTGGAGTGTTCATCACATTGGCGATATCTC
TTATGCTAGAGGTAAAGCATTCATTTGGGATTACTTTATGGATTCTATGCAACCAATCGT
CTCAAAAGTACCATACATGGTATCGATTGGTAATCATGAATACGATTTCATTGGTCAACC
ATTTGCACCATCTTGGTCAAACTATGGTAGTGATAGTGGTGGTGAATGTGGTGTACCATA
CAGTAAGCGTTTCCATATGACAGGTGCAGAGGATTCAACTCGTAACCTTTGGTTCTCCTA
TGAGAATGGACCAATTCATTTCACTGTAATGTCAGCTGAACATGATTTCCTTCCAGGTAG
TCCACAATTTGAATGGTTAAATAATGATTTAGCATCAGTTGACCGTGAAAAAACTCCATG
GGTAATCTTTAGTGGTCATCGTCCATTATATACCTCTGCATTACCAGAGGATTCAATTGG
TTCAATCACAGCTCTTCGTGAAGCTATTGAACCATTATTCCAAAAGTATGATGTTGATAT
GGCATTATGGGGTCATGTTCACATCTATGAACGTACTTGTGGTTTCATTGGTAATTTCAC
TTGTGCTGATAATGATAATGATGGTACCGTTCATGTTATCATTGGTATGGCTGGTAATAC
TTACTCTGTACCTTGGGAAGGCTCTGATATTAGCTCTGGTAATGGTCACGAAGATGAACC
AGAATGGTCAATCTTTAGAAGTATCTCTTATGGTCATGTAAGATTCTATGCAAATACAAC
CTCACTTTATTTGAATTTGTGGAATCATAGATCAATGTTCANACTCTTTTG
sequence update 2003. 5. 2
Translated Amino Acid sequence 
tvglleaktpgl*KNTAFFFYIFLFFFIFXIHXNSEXDIXVXXQIKKK---

---YLNYYFGSENDGMSAIQSFLSQPDNSDPSNSEAFVIGFGDLGTTFPYTALVETQYPA
SETIAAISQTISAPYGSSPFVRAMGKQSNSIDRLDPSQTPFWSVHHIGDISYARGKAFIW
DYFMDSMQPIVSKVPYMVSIGNHEYDFIGQPFAPSWSNYGSDSGGECGVPYSKRFHMTGA
EDSTRNLWFSYENGPIHFTVMSAEHDFLPGSPQFEWLNNDLASVDREKTPWVIFSGHRPL
YTSALPEDSIGSITALREAIEPLFQKYDVDMALWGHVHIYERTCGFIGNFTCADNDNDGT
VHVIIGMAGNTYSVPWEGSDISSGNGHEDEPEWSIFRSISYGHVRFYANTTSLYLNLWNH
RSMFXLF
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptggkntrtlkkhcffflyffiffyffxsyxf*xgykxnxxnkkk---

---ptliiilvvkmmvcqlfnhfyhnqiiathqipkhl*lvlvtlvphfliqhwsklniq
qvkqlqqfpkplvhhmvhhhlleqwvnkviqsid*iqvkhhfgvfitlaislmlevkhsf
gitlwilcnqssqkyhtwyrlvimntislvnhlhhlgqtmvvivvvnvvyhtvsvsi*qv
qriqlvtfgspmrmdqfisl*cqlnmisfqvvhnlng*imi*hqltvkklhg*slvvivh
yiplhyqriqlvqsqlfvkllnhysksmmliwhygvmftsmnvlvvslvislvlimimmv
pfmlslvwlviltlylgkalilalvmvtkmnqngqslevslmvm*dsmqiqphfi*icgi
idqcsxsf

Frame B:
tvglleaktpgl*KNTAFFFYIFLFFFIFXIHXNSEXDIXVXXQIKKK---

---lp*llfw**k*wyvsysiifittr**rpikfrsicnwfw*pwyhislysigrnsiss
k*nncsnfpnh*ctiwfitic*sng*tk*fnr*irsksntilecsshwryllc*r*sihl
gllygfyatnrlkstihgidw*s*irfhwstictilvklw***ww*mwctiq*afpydrc
rgfns*plvll*ewtnsfhcnvs*t*fpsr*sti*mvk**fsis*p*knsmgnl*wsssi
iylcitrgfnwfnhsss*sy*tiipkv*c*ygimgscshl*tylwfhw*fhlc*****wy
rscyhwygw*yllctlgrl*y*lw*wsrr*trmvnl*kyllwsckilckynltlfefves
*invxtll

Frame C:
llaywrqkhqdfkktllfffiffyfflffxfixilnxi*x*xxk*kk---

---YLNYYFGSENDGMSAIQSFLSQPDNSDPSNSEAFVIGFGDLGTTFPYTALVETQYPA
SETIAAISQTISAPYGSSPFVRAMGKQSNSIDRLDPSQTPFWSVHHIGDISYARGKAFIW
DYFMDSMQPIVSKVPYMVSIGNHEYDFIGQPFAPSWSNYGSDSGGECGVPYSKRFHMTGA
EDSTRNLWFSYENGPIHFTVMSAEHDFLPGSPQFEWLNNDLASVDREKTPWVIFSGHRPL
YTSALPEDSIGSITALREAIEPLFQKYDVDMALWGHVHIYERTCGFIGNFTCADNDNDGT
VHVIIGMAGNTYSVPWEGSDISSGNGHEDEPEWSIFRSISYGHVRFYANTTSLYLNLWNH
RSMFXLF
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH636 (AFH636Q) /CSM/AF/AFH6-B/AFH636Q.Seq.d/                       2167   0.0
VHB351 (VHB351Q) /CSM/VH/VHB3-C/VHB351Q.Seq.d/                       1340   0.0
AFN142 (AFN142Q) /CSM/AF/AFN1-B/AFN142Q.Seq.d/                       1203   0.0
SFI612 (SFI612Q) /CSM/SF/SFI6-A/SFI612Q.Seq.d/                       1191   0.0
VHE835 (VHE835Q) /CSM/VH/VHE8-B/VHE835Q.Seq.d/                        880   0.0
SSA523 (SSA523Q) /CSM/SS/SSA5-A/SSA523Q.Seq.d/                        688   0.0
VFH673 (VFH673Q) /CSM/VF/VFH6-D/VFH673Q.Seq.d/                         42   0.020
FC-AG08 (FC-AG08Q) /CSM/FC/FC-AG/FC-AG08Q.Seq.d/                       42   0.020
SHL287 (SHL287Q) /CSM/SH/SHL2-D/SHL287Q.Seq.d/                         40   0.080
CFB767 (CFB767Q) /CSM/CF/CFB7-C/CFB767Q.Seq.d/                         40   0.080
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF492661|AF492661.1 Arabidopsis thaliana purple acid phosphatase (PAP9) mRNA, complete cds.    54   0.001  2
AY090895|AY090895.1 Arabidopsis thaliana truncated putative purple acid phosphatase (PAP9) mRNA, complete cds, alternatively spliced.    54   0.001  2
AC006284|AC006284.4 Arabidopsis thaliana chromosome 2 clone T4M8 map CIC11A04, complete sequence.    54   0.005  1
AW697837|AW697837.1 EST0020 carnation flower specific cDNA library Dianthus caryophyllus cDNA clone HM020 5' similar to hypothetical protein, mRNA sequence.    54   0.005  1
AW697825|AW697825.1 EST0008 carnation flower specific cDNA library Dianthus caryophyllus cDNA clone HM008 5' similar to putative protein, mRNA sequence.    54   0.005  1
AE014826|AE014826.1 Plasmodium falciparum 3D7 chromosome 14 section 11 of 13 of the complete sequence.    40   0.012  11
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    38   0.015  10
BQ512128|BQ512128.1 EST619543 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMHW35 5' end, mRNA sequence.    38   0.016  3
BF521364|BF521364.1 EST458840 DSIL Medicago truncatula cDNA clone pDSIL-43M17, mRNA sequence.    46   0.082  2
CK274485|CK274485.1 EST720563 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POADH60 5' end, mRNA sequence.    40   0.10   2
dna update 2004. 5.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC068197_11(AC068197|pid:none) Genomic sequence for Arabidopsis ...   256   1e-66
AC006284_10(AC006284|pid:none) Arabidopsis thaliana chromosome 2...   254   3e-66
AM458569_4(AM458569|pid:none) Vitis vinifera contig VV78X180401....   253   7e-66
EU975503_1(EU975503|pid:none) Zea mays clone 490987 hydrolase/ p...   243   7e-63
AY090895_1(AY090895|pid:none) Arabidopsis thaliana truncated put...   241   4e-62
AK100372_1(AK100372|pid:none) Oryza sativa Japonica Group cDNA c...   240   6e-62
EF678036_1(EF678036|pid:none) Picea sitchensis clone WS02821_B10...   200   7e-50
EU956598_1(EU956598|pid:none) Zea mays clone 1567723 nucleotide ...   194   7e-48
AM466002_2(AM466002|pid:none) Vitis vinifera contig VV78X188747....   193   9e-48
AP003221_7(AP003221|pid:none) Oryza sativa Japonica Group genomi...   187   6e-46
protein update 2009. 3.28
PSORT 

 psg: 1.04  gvh: 0.60  alm: 0.27  top: 0.37  tms: 0.07  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for AFH636 is end
5' end seq. ID AFH636F
5' end seq. 
>AFH636F.Seq
CACTGTTGGCCTACTGGAGGCAAAAACACCAGGACTTTAAAAAAACACTGCTTTTTTTTT
TTATATTTTTTTATTTTTTTTTATTTTTTTNATTCATATNAATTCTGAATNGGATATAAA
NGTAATNTTNCAAATAAAAAAAAAA----------
Length of 5' end seq. 145
3' end seq. ID AFH636Z
3' end seq. 
>AFH636Z.Seq
NNNNNNNNNNCCTACCTTAATTATTATTTTGGTAGTGAAAATGATGGTATGTCAGCTATT
CAATCATTTTTATCACAACCAGATAATAGCGACCCATCAAATTCCGAAGCATTTGTAATT
GGTTTTGGTGACCTTGGTACCACATTTCCTTATACAGCATTGGTCGAAACTCAATATCCA
GCAAGTGAAACAATTGCAGCAATTTCCCAAACCATTAGTGCACCATATGGTTCATCACCA
TTTGTTAGAGCAATGGGTAAACAAAGTAATTCAATCGATAGATTAGATCCAAGTCAAACA
CCATTTTGGAGTGTTCATCACATTGGCGATATCTCTTATGCTAGAGGTAAAGCATTCATT
TGGGATTACTTTATGGATTCTATGCAACCAATCGTCTCAAAAGTACCATACATGGTATCG
ATTGGTAATCATGAATACGATTTCATTGGTCAACCATTTGCACCATCTTGGTCAAACTAT
GGTAGTGATAGTGGTGGTGAATGTGGTGTACCATACAGTAAGCGTTTCCATATGACAGGT
GCAGAGGATTCAACTCGTAACCTTTGGTTCTCCTATGAGAATGGACCAATTCATTTCACT
GTAATGTCAGCTGAACATGATTTCCTTCCAGGTAGTCCACAATTTGAATGGTTAAATAAT
GATTTAGCATCAGTTGACCGTGAAAAAACTCCATGGGTAATCTTTAGTGGTCATCGTCCA
TTATATACCTCTGCATTACCAGAGGATTCAATTGGTTCAATCACAGCTCTTCGTGAAGCT
ATTGAACCATTATTCCAAAAGTATGATGTTGATATGGCATTATGGGGTCATGTTCACATC
TATGAACGTACTTGTGGTTTCATTGGTAATTTCACTTGTGCTGATAATGATAATGATGGT
ACCGTTCATGTTATCATTGGTATGGCTGGTAATACTTACTCTGTACCTTGGGAAGGCTCT
GATATTAGCTCTGGTAATGGTCACGAAGATGAACCAGAATGGTCAATCTTTAGAAGTATC
TCTTATGGTCATGTAAGATTCTATGCAAATACAACCTCACTTTATTTGAATTTGTGGAAT
CATAGATCAATGTTCANACTCTTTTG
Length of 3' end seq. 1106
Connected seq. ID AFH636P
Connected seq. 
>AFH636P.Seq
CACTGTTGGCCTACTGGAGGCAAAAACACCAGGACTTTAAAAAAACACTGCTTTTTTTTT
TTATATTTTTTTATTTTTTTTTATTTTTTTNATTCATATNAATTCTGAATNGGATATAAA
NGTAATNTTNCAAATAAAAAAAAAA----------CCTACCTTAATTATTATTTTGGTAG
TGAAAATGATGGTATGTCAGCTATTCAATCATTTTTATCACAACCAGATAATAGCGACCC
ATCAAATTCCGAAGCATTTGTAATTGGTTTTGGTGACCTTGGTACCACATTTCCTTATAC
AGCATTGGTCGAAACTCAATATCCAGCAAGTGAAACAATTGCAGCAATTTCCCAAACCAT
TAGTGCACCATATGGTTCATCACCATTTGTTAGAGCAATGGGTAAACAAAGTAATTCAAT
CGATAGATTAGATCCAAGTCAAACACCATTTTGGAGTGTTCATCACATTGGCGATATCTC
TTATGCTAGAGGTAAAGCATTCATTTGGGATTACTTTATGGATTCTATGCAACCAATCGT
CTCAAAAGTACCATACATGGTATCGATTGGTAATCATGAATACGATTTCATTGGTCAACC
ATTTGCACCATCTTGGTCAAACTATGGTAGTGATAGTGGTGGTGAATGTGGTGTACCATA
CAGTAAGCGTTTCCATATGACAGGTGCAGAGGATTCAACTCGTAACCTTTGGTTCTCCTA
TGAGAATGGACCAATTCATTTCACTGTAATGTCAGCTGAACATGATTTCCTTCCAGGTAG
TCCACAATTTGAATGGTTAAATAATGATTTAGCATCAGTTGACCGTGAAAAAACTCCATG
GGTAATCTTTAGTGGTCATCGTCCATTATATACCTCTGCATTACCAGAGGATTCAATTGG
TTCAATCACAGCTCTTCGTGAAGCTATTGAACCATTATTCCAAAAGTATGATGTTGATAT
GGCATTATGGGGTCATGTTCACATCTATGAACGTACTTGTGGTTTCATTGGTAATTTCAC
TTGTGCTGATAATGATAATGATGGTACCGTTCATGTTATCATTGGTATGGCTGGTAATAC
TTACTCTGTACCTTGGGAAGGCTCTGATATTAGCTCTGGTAATGGTCACGAAGATGAACC
AGAATGGTCAATCTTTAGAAGTATCTCTTATGGTCATGTAAGATTCTATGCAAATACAAC
CTCACTTTATTTGAATTTGTGGAATCATAGATCAATGTTCANACTCTTTTG
Length of connected seq. 1241
Full length Seq ID -
Full length Seq. -
Length of full length seq. -