>AFH589 (AFH589Q) /CSM/AF/AFH5-D/AFH589Q.Seq.d/
XXXXXXXXXXCCATTCAATTGATGNAATTTGAAGATGCCTTAAAGTATCGTTTCAATCCA
TTCGATGTAACAAAGATTTGGTCACACAAAGACTATCCATTAATTCAAATTGGTCGTATG
GTTTTGAATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCA
TCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTC
TCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAAT
TGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTC
AATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCA
CATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATAC
AATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGAT
GCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAA
ATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTA
TGTAAGGGTCTTGGTATTGATGTTAATNATGTCATCAAATTCGATGCTAGATCAAACTTG
TAAATCAATA

---IQLMXFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLNRNPENYFAEVEQAAFSPS
HMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQRDGFMAVN
GNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLYRLMSEDA
KSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDVNXVIKFDARSNL*
in

Frame A:
---hsidxi*rclkvsfqsircnkdlvtqrlsinsnwsygfes*srellc*s*tscllti
phgsry*titr*dvtrsslllsryssssfrcklstnpsqlsirrkgwc*klpt*wfhgcq
w*ww*rsklstklfrwsratsricst*i*rlwfrcsstiqssk*rlcstr*fi*thv*rc
qisfrl*fsrshvrchhqrnssscrfkllqg*qrfrcsfm*gswy*c*xchqirc*iklv
nq


Frame B:
---IQLMXFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLNRNPENYFAEVEQAAFSPS
HMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQRDGFMAVN
GNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLYRLMSEDA
KSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDVNXVIKFDARSNL*
in


Frame C:
---fn*xnlkmp*sivsihsm*qrfghtktih*fklvvwf*iviqritllklnklpshhp
twfqvlnhhqircykvvssliqiliviv*v*tinksqsivhsp*rvvlkttnvmvswlsm
vmvvkvqtinqtlsvvqshiqnllninltslvsllvnhtiiqmttlfnqviyidsclkmp
nlvssli*svtcpvspskkfkfvpfqtftrltki*vlvyvrvlvlmlxmssnsmldqtck
si

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH589 (AFH589Q) /CSM/AF/AFH5-D/AFH589Q.Seq.d/                       1415   0.0
SFK640 (SFK640Q) /CSM/SF/SFK6-B/SFK640Q.Seq.d/                       1407   0.0
SFJ685 (SFJ685Q) /CSM/SF/SFJ6-D/SFJ685Q.Seq.d/                       1407   0.0
FC-142 (FC-142Q) /CSM/CG/CG-FC/FC-142Q.Seq.d/                        1407   0.0
CFI885 (CFI885Q) /CSM/CF/CFI8-D/CFI885Q.Seq.d/                       1407   0.0
CFF134 (CFF134Q) /CSM/CF/CFF1-B/CFF134Q.Seq.d/                       1407   0.0
CFC537 (CFC537Q) /CSM/CF/CFC5-B/CFC537Q.Seq.d/                       1407   0.0
AFN369 (AFN369Q) /CSM/AF/AFN3-C/AFN369Q.Seq.d/                       1407   0.0
AFM764 (AFM764Q) /CSM/AF/AFM7-C/AFM764Q.Seq.d/                       1407   0.0
AFM195 (AFM195Q) /CSM/AF/AFM1-D/AFM195Q.Seq.d/                       1407   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF090443|AF090443.1 Dictyostelium discoideum catalase (cat) mRNA, complete cds.  1407   0.0    1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1407   0.0    1
AJ400965|AJ400965.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, strain PR.    76   1e-16  2
AJ400964|AJ400964.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, wild type.    76   1e-16  2
AB064338|AB064338.1 Candida boidinii CTA1 gene for peroxisomal catalase, complete cds.    82   1e-16  2
AR375090|AR375090.1 Sequence 96 from patent US 6605709.            76   1e-16  2
E65314|E65314.1 Novel catalase, gene of said catalase and composition containing  said  catalase, and process for preparing catalase using the genetic engineering  technology.    86   8e-13  1
CD645756|CD645756.1 SSPG103 SS pectin Sclerotinia sclerotiorum cDNA similar to Peroxisomal catalase, mRNA sequence.    68   1e-12  2
CC133697|CC133697.1 NDL.83G19.SP6 Notre Dame Liverpool Aedes aegypti genomic clone NDL.83G19, DNA sequence.    46   2e-09  4
BX554380|BX554380.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse15b01_q1c.    74   3e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF090443_1(AF090443|pid:none) Dictyostelium discoideum catalase ...   487   e-136
CQ796333_1(CQ796333|pid:none) Sequence 109 from Patent WO2004027...   275   8e-73
AE017333_3935(AE017333|pid:none) Bacillus licheniformis DSM 13, ...   275   8e-73
CP000560_826(CP000560|pid:none) Bacillus amyloliquefaciens FZB42...   270   2e-71
CP000300_481(CP000300|pid:none) Methanococcoides burtonii DSM 62...   268   1e-70
(O93662) RecName: Full=Catalase;          EC=1.11.1.6;  &AJ00593...   262   7e-69
(P26901) RecName: Full=Vegetative catalase;          EC=1.11.1.6...   261   2e-68
AY378080_1(AY378080|pid:none) Tetrahymena thermophila putative c...   259   7e-68
(Q9PWF7) RecName: Full=Catalase;          EC=1.11.1.6;  &AB03187...   251   2e-65
AY242059_1(AY242059|pid:none) Melopsittacus undulatus catalase (...   246   4e-64

 psg: 0.75  gvh: 0.22  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFH589 is nuc

>AFH589Z.Seq
----------CCATTCAATTGATGNAATTTGAAGATGCCTTAAAGTATCGTTTCAATCCA
TTCGATGTAACAAAGATTTGGTCACACAAAGACTATCCATTAATTCAAATTGGTCGTATG
GTTTTGAATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCA
TCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTC
TCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAAT
TGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTC
AATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCA
CATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATAC
AATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGAT
GCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAA
ATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTA
TGTAAGGGTCTTGGTATTGATGTTAATNATGTCATCAAATTCGATGCTAGATCAAACTTG
TAAATCAATA