>AFH576 (AFH576Q) /CSM/AF/AFH5-D/AFH576Q.Seq.d/
XXXXXXXXXXGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTG
GTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCG
ATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTG
GTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTA
AGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGG
TTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAAT
TAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAA
ATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTG
ATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTG
AATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATNATA
ATGTNAATCTAAAAAAGATTCTTCGCTAA

---VDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGG
KVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKL
GEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVE
FIQDNAAIEFKLPSSQTDXNVNLKKILR*

Frame A:
---VDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGG
KVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKL
GEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVE
FIQDNAAIEFKLPSSQTDXNVNLKKILR*


Frame B:
---si*rnshnkqliwvslvklfqpslliqlpiklvtismkkkpshsipspngskmslvv
kflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*
vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tlln
sfkitlplnsnyhhlklximxi*krffa


Frame C:
---ryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*
sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir
*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*i
hsr*rch*iqitiisn*x*cxskkdssl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN190 (AFN190Q) /CSM/AF/AFN1-D/AFN190Q.Seq.d/                       1215   0.0
AFI881 (AFI881Q) /CSM/AF/AFI8-D/AFI881Q.Seq.d/                       1215   0.0
AFI417 (AFI417Q) /CSM/AF/AFI4-A/AFI417Q.Seq.d/                       1215   0.0
AFH576 (AFH576Q) /CSM/AF/AFH5-D/AFH576Q.Seq.d/                       1215   0.0
AFO770 (AFO770Q) /CSM/AF/AFO7-C/AFO770Q.Seq.d/                       1207   0.0
SFL246 (SFL246Q) /CSM/SF/SFL2-B/SFL246Q.Seq.d/                       1205   0.0
SFK855 (SFK855Q) /CSM/SF/SFK8-C/SFK855Q.Seq.d/                       1205   0.0
SFI119 (SFI119Q) /CSM/SF/SFI1-A/SFI119Q.Seq.d/                       1205   0.0
AFO809 (AFO809Q) /CSM/AF/AFO8-A/AFO809Q.Seq.d/                       1205   0.0
AFO715 (AFO715Q) /CSM/AF/AFO7-A/AFO715Q.Seq.d/                       1205   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   4e-08  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   4e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   4e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   4e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   168   1e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   164   2e-39
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   162   9e-39
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   161   2e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   157   3e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   1e-36
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   152   6e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   1e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   149   5e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   149   5e-35

 psg: 0.84  gvh: 0.38  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for AFH576 is cyt

>AFH576Z.Seq
----------GGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTG
GTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCG
ATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTG
GTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTA
AGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGG
TTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAAT
TAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAA
ATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTG
ATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTG
AATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATNATA
ATGTNAATCTAAAAAAGATTCTTCGCTAA