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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH563
Library AF
(Link to library)
Clone ID AFH563
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFH563P
(Link to Original site)
Representative DNA sequence 
>AFH563 (AFH563Q) /CSM/AF/AFH5-C/AFH563Q.Seq.d/
CACTGTTGGCCTACTGGNATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAA
AATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGC
CGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAG
ACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCA
ATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTG
GGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGA
AGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAAT
GACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGT
CTTATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGT
CTCCCACACTGTTCCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGA
TTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGXXXXXXXXXXGTC
GTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCA
CTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACT
TTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTAC
CAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCC
AACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTATAACTCCATCA
TGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCTGGTGGTA
CAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCAT
CAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTG
GATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATG
ACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTGATG
AAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
tvgllx*f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPR
HTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPE
EHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGV
SHTVPIYEGYALPHAILRLDLAGRDLTDYMMKILT---

---RDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKS
YELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVL
SGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK
EEYDESGPSIVHRKCF*tiktsdesashtnkyi*mynnnki
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgivlinkylniiqq*ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwssk
thwcygrygskrllrr**spikerylnpqipn*trycyqlg*ygknlasyflq*tpccsr
rtpssln*spikskgqqrkndpnyv*nlqhpshvrchsscliliclwsyhrycygfr*wc
lphcsnl*rlclttchltfrfsws*sy*lhdenln---

---vvillit**ks*lnvvthsppllkeksseiskkn*ptspstlklkckllphhqpskn
htnyqtvksspsvmnvsvaqkpssnhhs*vwnqlvstkppitps*nvmlisvkiytvmss
ylvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgsp
kknmtnqvhqlstenvskqlklvmkvlltqtnifkciiiiky

Frame B:
tvgllx*f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPR
HTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPE
EHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGV
SHTVPIYEGYALPHAILRLDLAGRDLTDYMMKILT---

---s*sy*lhdenln*twllihhhc*krnrqryqrkislrrprl*s*nanccliisprki
iritrrsshhhr**tfplprsplptiilrygiswyprnhl*lhhem*c*yp*rfir*crl
iwwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlq
rri*rirsincpqkmflnn*n***kcfshkqiylnv****n

Frame C:
llaywxsfnkqifkynttikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqd
tlvlwsvwvkktlt*vmkpnqrevs*psntqlntvllptgmiwkksgiilstmnsvllqk
ntqfs*lkph*iqrptekk*pklclkpstpqpctlpfklsypymplvvppvllwiqvmvs
ptlfqsmkvmpyhmpsyv*i*lvvillit**ks*l---

---RDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKS
YELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVL
SGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK
EEYDESGPSIVHRKCF*tiktsdesashtnkyi*mynnnki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH563 (AFH563Q) /CSM/AF/AFH5-C/AFH563Q.Seq.d/                       2539   0.0
VFD651 (VFD651Q) /CSM/VF/VFD6-C/VFD651Q.Seq.d/                       1302   0.0
SLC191 (SLC191Q) /CSM/SL/SLC1-D/SLC191Q.Seq.d/                       1302   0.0
SLB167 (SLB167Q) /CSM/SL/SLB1-C/SLB167Q.Seq.d/                       1302   0.0
VFL589 (VFL589Q) /CSM/VF/VFL5-D/VFL589Q.Seq.d/                       1300   0.0
VFE460 (VFE460Q) /CSM/VF/VFE4-C/VFE460Q.Seq.d/                       1300   0.0
VFE392 (VFE392Q) /CSM/VF/VFE3-D/VFE392Q.Seq.d/                       1300   0.0
VFE112 (VFE112Q) /CSM/VF/VFE1-A/VFE112Q.Seq.d/                       1300   0.0
SFH413 (SFH413Q) /CSM/SF/SFH4-A/SFH413Q.Seq.d/                       1300   0.0
SFG507 (SFG507Q) /CSM/SF/SFG5-A/SFG507Q.Seq.d/                       1300   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1302   0.0    9
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1136   0.0    8
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      1094   0.0    1
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        1033   0.0    3
AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1033   0.0    7
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   898   0.0    13
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   896   0.0    9
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.    650   0.0    2
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.  1001   0.0    1
AF508263|AF508263.1 Pyrocystis lunula actin mRNA, complete cds.   603   0.0    3
dna update 2004. 5.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P27132) RecName: Full=Actin-2; AltName: Full=Actin II; Flags: F...   350   e-176
CR855972_1(CR855972|pid:none) Paramecium tetraurelia macronuclea...   244   e-124
BC075201_1(BC075201|pid:none) Xenopus laevis ARP1 actin-related ...   246   e-114
AB170491_1(AB170491|pid:none) Macaca fascicularis brain cDNA clo...   247   e-113
AB169693_1(AB169693|pid:none) Macaca fascicularis brain cDNA, cl...   246   e-113
CR855988_1(CR855988|pid:none) Paramecium tetraurelia macronuclea...   218   e-110
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   400   e-110
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   400   e-110
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   398   e-109
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   397   e-109
protein update 2009. 4.21
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFH563 is cyt
5' end seq. ID AFH563F
5' end seq. 
>AFH563F.Seq
CACTGTTGGCCTACTGGNATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAA
AATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGC
CGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAG
ACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCA
ATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTG
GGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGA
AGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAAT
GACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGT
CTTATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGT
CTCCCACACTGTTCCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGA
TTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTG----------
Length of 5' end seq. 647
3' end seq. ID AFH563Z
3' end seq. 
>AFH563Z.Seq
----------GTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTAC
TCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTAC
GTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAA
TCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCA
GAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACC
TATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTC
TTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACT
GCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCT
GTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCC
AAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATT
AAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAAT
AAAATAT
Length of 3' end seq. 657
Connected seq. ID AFH563P
Connected seq. 
>AFH563P.Seq
CACTGTTGGCCTACTGGNATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAA
AATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGC
CGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAG
ACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCA
ATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTG
GGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGA
AGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAAT
GACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGT
CTTATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGT
CTCCCACACTGTTCCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGA
TTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTG----------GTC
GTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCA
CTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACT
TTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTAC
CAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCC
AACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTATAACTCCATCA
TGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCTGGTGGTA
CAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCAT
CAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTG
GATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATG
ACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTGATG
AAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATAT
Length of connected seq. 1304
Full length Seq ID -
Full length Seq. -
Length of full length seq. -