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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
FC-IC0300
Library FC-IC
(Link to library)
Clone ID FC-IC0300
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14349-1
Original site URL
Representative seq. ID FC-IC0300E
(Link to Original site)
Representative DNA sequence 
>FC-IC0300 (FC-IC0300Q) /CSM/FC-IC/FC-IC0300Q.Seq.d/
CTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCC
AAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTA
CAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTC
ATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTA
TCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGA
TACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGC
CTGTGTTGGTATCCAAGTTGAAATCCAAGGT
sequence update 2001. 6.12
Translated Amino Acid sequence 
lln*iknyi*KMSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCS
FDEFTKCLPENECRYVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKA
CVGIQVEIQG
Translated Amino Acid sequence (All Frames) 
Frame A:
ltqlnkklyiknvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlf
ir*ihqmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*s
lcwyps*npr


Frame B:
lln*iknyi*KMSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCS
FDEFTKCLPENECRYVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKA
CVGIQVEIQG


Frame C:
ystk*ktiykkclqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvvh
smnspnvsqktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktlyvkp
vlvsklksk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ890 (VSJ890Q) /CSM/VS/VSJ8-D/VSJ890Q.Seq.d/                        706   0.0
VSJ584 (VSJ584Q) /CSM/VS/VSJ5-D/VSJ584Q.Seq.d/                        706   0.0
VSJ550 (VSJ550Q) /CSM/VS/VSJ5-C/VSJ550Q.Seq.d/                        706   0.0
VSJ437 (VSJ437Q) /CSM/VS/VSJ4-B/VSJ437Q.Seq.d/                        706   0.0
VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/                        706   0.0
VSI135 (VSI135Q) /CSM/VS/VSI1-B/VSI135Q.Seq.d/                        706   0.0
VSG665 (VSG665Q) /CSM/VS/VSG6-C/VSG665Q.Seq.d/                        706   0.0
VSE444 (VSE444Q) /CSM/VS/VSE4-B/VSE444Q.Seq.d/                        706   0.0
VSD383 (VSD383Q) /CSM/VS/VSD3-D/VSD383Q.Seq.d/                        706   0.0
VSD320 (VSD320Q) /CSM/VS/VSD3-A/VSD320Q.Seq.d/                        706   0.0
own update 2001. 8.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D37981|D37981.1 Dictyostelium discoidium DDCOF2 gene for cofilin, complete cds.   706   0.0    1
D37980|D37980.1 Dictyostelium discoidium DDCOF1 gene for cofilin, complete cds (exon1-2).   704   0.0    1
X04004|X04004.1 Dictyostelium discoideum mRNA for contact site A (csA) protein.   168   3e-38  1
AC135132|AC135132.2 Rattus norvegicus clone CH230-240G18, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    44   1.4    1
J05087|J05087.1 Rat calmodulin-sensitive plasma membrane Ca2+-transporting ATPase (PMCA3) mRNA, complete cds.    44   1.4    1
BQ108127|BQ108127.1 shrpID000306 LvG Litopenaeus vannamei cDNA, mRNA sequence.    44   1.4    1
CB579150|CB579150.1 AMGNNUC:NRHY7-00010-A11-A nrhy7 (10850) Rattus norvegicus cDNA clone nrhy7-00010-a11 5', mRNA sequence.    44   1.4    1
BZ859527|BZ859527.1 CH240_269E4.TJ CHORI-240 Bos taurus genomic clone CH240_269E4, DNA sequence.    44   1.4    1
AC115450|AC115450.3 Rattus norvegicus clone CH230-210N1, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.    44   1.4    1
AC096403|AC096403.6 Rattus norvegicus clone CH230-31P8, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    44   1.4    1
dna update 2003.12.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54706) RecName: Full=Cofilin-1;  &D37980_1(D37980|pid:none) &D...   246   2e-64
(P37167) RecName: Full=Actophorin;  &M93361_1(M93361|pid:none)        119   4e-26
(Q96VU9) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   115   5e-25
FM992692_38(FM992692|pid:none) Candida dubliniensis CD36 chromos...   114   1e-24
AY428595_1(AY428595|pid:none) Helianthus annuus clone HA10-7 put...   114   1e-24
CP000501_299(CP000501|pid:none) Pichia stipitis CBS 6054 chromos...   111   9e-24
(Q9HF97) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   110   2e-23
(Q6CQ22) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   109   3e-23
EU961226_1(EU961226|pid:none) Zea mays clone 233661 actin-depoly...   108   4e-23
BT087663_1(BT087663|pid:none) Zea mays full-length cDNA clone ZM...   108   6e-23
protein update 2009. 7. 1
PSORT 

 psg: 0.91  gvh: 0.46  alm: 0.50  top: 0.60  tms: 0.00  mit: 0.30  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for FC-IC0300 is cyt
5' end seq. ID FC-IC0300F
5' end seq. 
>FC-IC0300F.Seq
CTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCC
AAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTA
CAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTC
ATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTA
TCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGA
TACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGC
CTGTGTTGGTATCCAAGTTGAAATCCAAGGT----------
Length of 5' end seq. 391
3' end seq. ID FC-IC0300Z
3' end seq. 
>FC-IC0300Z.Seq
----------CTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTG
CTTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTG
GTATCATCTACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAG
CTGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTG
TCCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCT
GGTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTT
TACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAGGT
Length of 3' end seq. 391
Connected seq. ID FC-IC0300P
Connected seq. 
>FC-IC0300P.Seq
CTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCC
AAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTA
CAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTC
ATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTA
TCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGA
TACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGC
CTGTGTTGGTATCCAAGTTGAAATCCAAGGT----------CTTACTCAACTAAATAAAA
AACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTC
AACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCA
AAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAA
TGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGT
GCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAG
AAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTT
GAAATCCAAGGT
Length of connected seq. 782
Full length Seq ID FC-IC0300E
Full length Seq. 
>FC-IC0300E.Seq
CTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCC
AAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTA
CAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTC
ATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTA
TCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGA
TACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGC
CTGTGTTGGTATCCAAGTTGAAATCCAAGGT
Length of full length seq. 391