>FC-IC0067 (FC-IC0067Q) /CSM/FC-IC/FC-IC0067Q.Seq.d/
ACATATATAATAATTTTTGTGTGAGAAGCACTTTCATCACTAAATTTAGAAACATTTTCT
GTGAACAATTGATGGACCAGATTCGTCATATTCTTCTTTTGAAATCCACATTTGTTGGAA
AGTTGAGAGTGAAGCTAAAATAGATCCACCAATCCAGACTGAGTATTTACGTTCTGGTGG
TGCAATGATTTTGATTTTCATTGTTGATGGTGCTAATGCAGTTAATTCTTTGTTCATACG
ATCTGCAATACCTGGGAACATAGTTGTXXXXXXXXXX

hi**flcekhfhh*i*khfl*TIDGPDSSYSSFEIHICWKVESEAKIDPPIQTEYLRSGG
AMILIFIVDGANAVNSLFIRSAIPGNIV---

Frame A:
tyiiifv*ealsslnletfsvnn*wtrfvifff*nphlles*e*s*nrstnpd*vftfww
cndfdfhc*wc*cs*ffvhticntwehs---


Frame B:
hi**flcekhfhh*i*khfl*TIDGPDSSYSSFEIHICWKVESEAKIDPPIQTEYLRSGG
AMILIFIVDGANAVNSLFIRSAIPGNIV---


Frame C:
iynnfcvrstfitkfrnifceqlmdqirhilllkstfvgklrvklk*ihqsrlsiyvlvv
q*f*fsllmvlmqlilcsydlqylgt*l---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK236 (VFK236Q) /CSM/VF/VFK2-B/VFK236Q.Seq.d/                        529   e-150
VFC286 (VFC286Q) /CSM/VF/VFC2-D/VFC286Q.Seq.d/                        529   e-150
SLF263 (SLF263Q) /CSM/SL/SLF2-C/SLF263Q.Seq.d/                        529   e-150
SLF220 (SLF220Q) /CSM/SL/SLF2-A/SLF220Q.Seq.d/                        529   e-150
SLA481 (SLA481Q) /CSM/SL/SLA4-D/SLA481Q.Seq.d/                        529   e-150
FC-IC1023 (FC-IC1023Q) /CSM/FC-IC/FC-IC1023Q.Seq.d/                   529   e-150
FC-IC0067 (FC-IC0067Q) /CSM/FC-IC/FC-IC0067Q.Seq.d/                   529   e-150
FC-AH11 (FC-AH11Q) /CSM/FC/FC-AH/FC-AH11Q.Seq.d/                      529   e-150
FC-IC0064 (FC-IC0064Q) /CSM/FC-IC/FC-IC0064Q.Seq.d/                   525   e-148
VFB424 (VFB424Q) /CSM/VF/VFB4-A/VFB424Q.Seq.d/                        523   e-148

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   529   0.0    3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   381   0.0    5
K02954|K02954.1 Slime mold (D.discoideum) actin 2-sub 1 gene, 3' end.   424   e-137  2
X03282|X03282.1 dictyostelium discoideum gene for actin A12.      440   e-120  1
AX771242|AX771242.1 Sequence 5 from Patent WO03034275.            373   e-113  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   373   e-102  2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.         373   e-102  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   347   e-102  2
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   379   e-101  4
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   371   3e-99  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   151   8e-36
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   151   8e-36
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   151   8e-36
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        151   8e-36
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   149   2e-35
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   149   4e-35
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   149   4e-35
AY582843_1(AY582843|pid:none) Amoebidium parasiticum actin gene,...   149   4e-35
(Q554S6) RecName: Full=Actin-17; AltName: Full=Actin-2-sub 2;  &...   149   4e-35
K02960_1(K02960|pid:none) Dictyostelium discoideum actin 8 gene,...   148   5e-35

 psg: 0.75  gvh: 0.68  alm: 0.31  top: 0.60  tms: 0.07  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for FC-IC0067 is nuc

>FC-IC0067F.Seq
ACATATATAATAATTTTTGTGTGAGAAGCACTTTCATCACTAAATTTAGAAACATTTTCT
GTGAACAATTGATGGACCAGATTCGTCATATTCTTCTTTTGAAATCCACATTTGTTGGAA
AGTTGAGAGTGAAGCTAAAATAGATCCACCAATCCAGACTGAGTATTTACGTTCTGGTGG
TGCAATGATTTTGATTTTCATTGTTGATGGTGCTAATGCAGTTAATTCTTTGTTCATACG
ATCTGCAATACCTGGGAACATAGTTGT----------