>CHS487 (CHS487Q) /CSM/CH/CHS4-D/CHS487Q.Seq.d/
XXXXXXXXXXACTTTAGATGGTGACAACGTCAATGGTTTCCCAAAGACTGTTACCGTTAA
ACCAGCCCCATCCGCTGAACACTCTTATGCTGAAGGTGAAGGTTTAGTCAAAGTATTTGA
TAATGCCCCAGCTGAATTCACTATTTTCGCCGTTGACACTAAAGGTGTTGCTCGTACCGA
TGGTGGTGATCCATTTGAAGTCGCTATCAATGGTCCAGATGGTTTAGTCGTTGATGCCAA
AGTTACCGATAACAATGACGGTACTTATGGTGTTGTCTATGATGCCCCAGTTGAAGGTAA
CTACAATGTTAATGTCACCCTCCGTGGTAATCCAATCAAAAATATGCCAATCGATGTCAA
ATGCATTGAAGGTGCCAATGGTGAAGATTCATCATTCGGTTCATTCACTTTTACCGTCGC
TGCTAAAAATAAGAAAGGTGAAGTTAAAACCTATGGTGGTGATAAATTTGAAGTCTCTAT
CACTGGTCCAGCTGAAGAAATCACTCTCGATGCTATTGATAACCAAGATGGTACTTATAC
TGCCGCTTACTCTTTAGTTGGTAATGGTCGTTTCTCAACTGGTGTCAAATTAAAT

---LDGDNVNGFPKTVTVKPAPSAEHSYAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDG
GDPFEVAINGPDGLVVDAKVTDNNDGTYGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKC
IEGANGEDSSFGSFTFTVAAKNKKGEVKTYGGDKFEVSITGPAEEITLDAIDNQDGTYTA
AYSLVGNGRFSTGVKLN

Frame A:
---l*mvttsmvsqrllplnqphplntlmlkvkv*skylimpqlnslfspltlkvllvpm
vvihlkslsmvqmv*slmpklpitmtvlmvlsmmpqlkvttmlmspsvviqskicqsmsn
alkvpmvkihhsvhsllpsllkirkvklkpmvvinlkslslvqlkkslsmllitkmvlil
pltl*lvmvvsqlvsn*


Frame B:
---frw*qrqwfpkdcyr*tspir*tllc*r*rfsqsi**cps*ihyfrr*h*rccsyrw
w*si*sryqwsrwfsr*cqsyr*q*rylwccl*cps*r*lqc*chppw*snqkyanrcqm
h*rcqw*rfiirfihfyrrc*k*er*s*nlww**i*slyhwss*rnhsrcy**prwylyc
rllfsw*wsflnwcqik


Frame C:
---LDGDNVNGFPKTVTVKPAPSAEHSYAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDG
GDPFEVAINGPDGLVVDAKVTDNNDGTYGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKC
IEGANGEDSSFGSFTFTVAAKNKKGEVKTYGGDKFEVSITGPAEEITLDAIDNQDGTYTA
AYSLVGNGRFSTGVKLN

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS487 (CHS487Q) /CSM/CH/CHS4-D/CHS487Q.Seq.d/                       1160   0.0
VFK681 (VFK681Q) /CSM/VF/VFK6-D/VFK681Q.Seq.d/                       1158   0.0
SLH892 (SLH892Q) /CSM/SL/SLH8-D/SLH892Q.Seq.d/                       1158   0.0
SFH146 (SFH146Q) /CSM/SF/SFH1-B/SFH146Q.Seq.d/                       1158   0.0
SFD611 (SFD611Q) /CSM/SF/SFD6-A/SFD611Q.Seq.d/                       1158   0.0
CHQ595 (CHQ595Q) /CSM/CH/CHQ5-D/CHQ595Q.Seq.d/                       1158   0.0
CHO879 (CHO879Q) /CSM/CH/CHO8-D/CHO879Q.Seq.d/                       1158   0.0
AHF883 (AHF883Q) /CSM/AH/AHF8-D/AHF883Q.Seq.d/                       1158   0.0
AHE344 (AHE344Q) /CSM/AH/AHE3-B/AHE344Q.Seq.d/                       1158   0.0
AHD645 (AHD645Q) /CSM/AH/AHD6-B/AHD645Q.Seq.d/                       1158   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X15430|X15430.1 Dictyostelium discoideum mRNA for gelation factor.  1158   0.0    1
AY382663|AY382663.1 Hirudo medicinalis filamin mRNA, complete cds.    40   1.4    2
DV482383|DV482383.1 5380_H09_O18Z_065 Brachypodium distachyon leaf EST library Brachypodium distachyon cDNA clone 5380_H09_O18, mRNA sequence.    44   3.3    1
BF983023|BF983023.1 602308354F1 NIH_MGC_88 Homo sapiens cDNA clone IMAGE:4399645 5', mRNA sequence.    44   3.3    1
CB142294|CB142294.1 K-EST0196054 L11SNU354s1 Homo sapiens cDNA clone L11SNU354s1-21-F05 5', mRNA sequence.    44   3.3    1
CB127416|CB127416.1 K-EST0176702 C1SNU17 Homo sapiens cDNA clone C1SNU17-19-G07 5', mRNA sequence.    44   3.3    1
CB128225|CB128225.1 K-EST0177627 L11SNU354 Homo sapiens cDNA clone L11SNU354-14-E03 5', mRNA sequence.    44   3.3    1
CB141970|CB141970.1 K-EST0195649 L11SNU354s1 Homo sapiens cDNA clone L11SNU354s1-31-C11 5', mRNA sequence.    44   3.3    1
CB108912|CB108912.1 K-EST0149954 L3SNU475 Homo sapiens cDNA clone L3SNU475-29-E09 5', mRNA sequence.    44   3.3    1
CB106467|CB106467.1 K-EST0143849 L3SNU475 Homo sapiens cDNA clone L3SNU475-4-F12 5', mRNA sequence.    44   3.3    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF080395_1(AF080395|pid:none) Entamoeba histolytica actin bindin...    93   5e-18
U70560_1(U70560|pid:none) Entamoeba histolytica actin binding pr...    74   4e-12
AK131179_1(AK131179|pid:none) Mus musculus mRNA for mFLJ00343 pr...    72   1e-11
BC151024_1(BC151024|pid:none) Mus musculus filamin, alpha, mRNA ...    72   1e-11
AL807376_2(AL807376|pid:none) Mouse DNA sequence from clone RP23...    72   1e-11
FJ416060_1(FJ416060|pid:none) Rattus norvegicus filamin alpha (F...    72   1e-11
AL807376_1(AL807376|pid:none) Mouse DNA sequence from clone RP23...    72   1e-11
AL807376_3(AL807376|pid:none) Mouse DNA sequence from clone RP23...    72   1e-11
(Q8BTM8) RecName: Full=Filamin-A; AltName: Full=Alpha-filamin; A...    72   1e-11
AF260122_1(AF260122|pid:none) Bos taurus filamin-1 mRNA, partial...    70   4e-11

 psg: 0.75  gvh: 0.32  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHS487 is cyt

>CHS487Z.Seq
NNNNNNNNNNACTTTAGATGGTGACAACGTCAATGGTTTCCCAAAGACTGTTACCGTTAA
ACCAGCCCCATCCGCTGAACACTCTTATGCTGAAGGTGAAGGTTTAGTCAAAGTATTTGA
TAATGCCCCAGCTGAATTCACTATTTTCGCCGTTGACACTAAAGGTGTTGCTCGTACCGA
TGGTGGTGATCCATTTGAAGTCGCTATCAATGGTCCAGATGGTTTAGTCGTTGATGCCAA
AGTTACCGATAACAATGACGGTACTTATGGTGTTGTCTATGATGCCCCAGTTGAAGGTAA
CTACAATGTTAATGTCACCCTCCGTGGTAATCCAATCAAAAATATGCCAATCGATGTCAA
ATGCATTGAAGGTGCCAATGGTGAAGATTCATCATTCGGTTCATTCACTTTTACCGTCGC
TGCTAAAAATAAGAAAGGTGAAGTTAAAACCTATGGTGGTGATAAATTTGAAGTCTCTAT
CACTGGTCCAGCTGAAGAAATCACTCTCGATGCTATTGATAACCAAGATGGTACTTATAC
TGCCGCTTACTCTTTAGTTGGTAATGGTCGTTTCTCAACTGGTGTCAAATTAAAT