>CHS473 (CHS473Q) /CSM/CH/CHS4-D/CHS473Q.Seq.d/
AGTACGAAAATATAACATTACATTATATAATTACAAAAATAAAATAAATAAAAATTGTAA
AACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANGGNNNNAAAAAAXXXXXXXX
XXCATTGTAAAGNNAACTGTNGTTCAAGTAANCNTTNNGAATGGTAGTATCAGTATNANN
GNATANNATGGTTTACAATTCACACTTCAATATATACCTAACAATGGTTATATCAGCGGT
GGT

VRKYNITLYNYKNKINKNCKTKKKKKKKKKKKXGXKK---

---IVKXTVVQVXXXNGSISXXXXXGLQFTLQYIPNNGYISGG

Frame A:
stki*hyii*lqk*nk*kl*nqkkkkkkkkkkkxxxk---

---hckxncxssxxxew*yqyxxixwftihtsiyt*qwlyqrw

Frame B:
VRKYNITLYNYKNKINKNCKTKKKKKKKKKKKXGXKK---

---IVKXTVVQVXXXNGSISXXXXXGLQFTLQYIPNNGYISGG

Frame C:
yenitlhyiitkik*ikivkpkkkkkkkkkkkxxxk---

---l*xxlxfk*xxxmvvsvxxxxmvynshfniyltmvisav

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS473 (CHS473Q) /CSM/CH/CHS4-D/CHS473Q.Seq.d/                        163   1e-39
CHR508 (CHR508Q) /CSM/CH/CHR5-A/CHR508Q.Seq.d/                        147   9e-35
CHQ303 (CHQ303Q) /CSM/CH/CHQ3-A/CHQ303Q.Seq.d/                        147   9e-35
CHN695 (CHN695Q) /CSM/CH/CHN6-D/CHN695Q.Seq.d/                        145   3e-34
CHN240 (CHN240Q) /CSM/CH/CHN2-B/CHN240Q.Seq.d/                        145   3e-34
CHM778 (CHM778Q) /CSM/CH/CHM7-D/CHM778Q.Seq.d/                        145   3e-34
CHM470 (CHM470Q) /CSM/CH/CHM4-C/CHM470Q.Seq.d/                        145   3e-34
CHM185 (CHM185Q) /CSM/CH/CHM1-D/CHM185Q.Seq.d/                        145   3e-34
CHH207 (CHH207Q) /CSM/CH/CHH2-A/CHH207Q.Seq.d/                        145   3e-34
CHL595 (CHL595Q) /CSM/CH/CHL5-D/CHL595Q.Seq.d/                        137   8e-32

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BX935907|BX935907.1 Gallus gallus finished cDNA, clone ChEST109f18.    46   0.32   1
AC125177|AC125177.4 Mus musculus chromosome 8 clone RP23-315H8, complete sequence.    44   1.3    1
AC118474|AC118474.10 Mus musculus chromosome 8, clone RP23-288O12, complete sequence.    44   1.3    1
AU303866|AU303866.1 Zinnia elegans cDNA, clone:Z12828.             42   5.0    1
AC146993|AC146993.2 Lytechinus variegatus clone BLV-223C1, WORKING DRAFT SEQUENCE, 4 ordered pieces.    42   5.0    1
AY136628|AY136628.2 Petunia integrifolia subsp. inflata S2 self-incompatibility ribonuclease (S2-RNase) and S2-locus F-box protein (SLF2) genes, complete cds.    42   5.0    1
AY016291|AY016291.1 Homo sapiens type V preprocollagen alpha 2 chain (COL5A2) gene, exons 7 through 14.    42   5.0    1
AC133106|AC133106.3 Homo sapiens chromosome 2 clone RP11-634B17, complete sequence.    42   5.0    1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 167,584,217

 psg: 0.71  gvh: 0.42  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.51  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: cytoplasmic

>> prediction for CHS473 is nuc

>CHS473F.Seq
AGTACGAAAATATAACATTACATTATATAATTACAAAAATAAAATAAATAAAAATTGTAA
AACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANGGNNNNAAAAAANNNNNNNN
NN

>CHS473Z.Seq
NNNNNNNNNNCATTGTAAAGNNAACTGTNGTTCAAGTAANCNTTNNGAATGGTAGTATCA
GTATNANNGNATANNATGGTTTACAATTCACACTTCAATATATACCTAACAATGGTTATA
TCAGCGGTGGT

>CHS473P.Seq
AGTACGAAAATATAACATTACATTATATAATTACAAAAATAAAATAAATAAAAATTGTAA
AACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANGGNNNNAAAAAA--------
--CATTGTAAAGNNAACTGTNGTTCAAGTAANCNTTNNGAATGGTAGTATCAGTATNANN
GNATANNATGGTTTACAATTCACACTTCAATATATACCTAACAATGGTTATATCAGCGGT
GGT