>CHS471 (CHS471Q) /CSM/CH/CHS4-C/CHS471Q.Seq.d/
ATTATTGGTGATACTATTTGGATTGATTCAAATAATAATTCACTATTAGACAATGGTGAA
GTTGGTGCAGATGGTATTAAAGTTGAATTATTAGTATCTTCAGATCTTTCACCAGCATTG
GATATTTTAGGTAAACCAATTTCAAGTCAAGTAACAACTGCCAATGGTAAATATTTATTC
CAAAATGTATCAAGTGGACCTTCCTATGTAGTAAGATTAACTATACCTGCCAATAGTACC
AAATATATTGCAGGTACTACTTATTCACCAATTTTGACAGGTGGTATGTCAAGAGCCACA
GCAAACGGTCAAGTAATTGTTTTGAATAACTTTACATTGGATACAACCTATGGATCAAAT
ACTTGGAAAAATCTAAACAATGATGCAGGTATTGTAATCCCATGTTACAGTATTGGTCAA
CAATCATGGTCCGATGGTAATCTCAATGGTATCTTTGATATCAATGNAATTGGTTTCCCA
TATGTAAACCTTACACTCTTTAATGCAGATGGTTCCACTCCAAATGATATTTTCGGCAAA
CCAATTCAAATGGCAXXXXXXXXXXGGTACTGGTGAATTCTATAATGATAACTTTAGAAA
AGGTCAAATTGGTCATGATGATACTGGTGGTCTTTCAGTTTTCCAAGTTCCAGGTTTCCC
AGAGGTTGCATCAGCTTCATTCGATGCTACAACTGTATTCGAAGGTGTAGTCAAATTTTA
TAACAATAACAATGGTACCCTTCGTTCTTCATTCCAAGTTTATCTCACTGATAATTCAGA
TACAGTCAATCCAGTTACCTTTGGCAAAGCATCGGGTCTTGGTCAACTTGTAGCCCACTG
TTCTCCAAAACCAATTACCCTTGGTAGTATTGTCTTTATTGATACAAATGGTAATGGTAT
TCAAGAACCATGGGAGCAAGGTAAACAAGGTGTTGCCGTTAGTTTATTATTTGCCAATGG
TACCTTAATTCAAAAACAATCAACCGATTCATTAGGTCTATTTAAATTCATTAATCCACC
ATACCAAAATCAACAATATATTATTACAGCTGATTCAGTTACACTTTCAATTATTCCATC
ACCTCTACCAACTTCATCTATTCCTTATAACGCTGCAACAATGGTTAATGGAAAAGCAAC
AATTTCAAATATTCTTATTTTAGATCCAATGATAGCTTCAAGTAGATATNATTTAAATTT
TGGTGTTACAAACTAAAAAATTAAAAAAATTAAAAAATTAAAAAATTAAA

IIGDTIWIDSNNNSLLDNGEVGADGIKVELLVSSDLSPALDILGKPISSQVTTANGKYLF
QNVSSGPSYVVRLTIPANSTKYIAGTTYSPILTGGMSRATANGQVIVLNNFTLDTTYGSN
TWKNLNNDAGIVIPCYSIGQQSWSDGNLNGIFDINXIGFPYVNLTLFNADGSTPNDIFGK
PIQMA---

---GTGEFYNDNFRKGQIGHDDTGGLSVFQVPGFPEVASASFDATTVFEGVVKFYNNNNG
TLRSSFQVYLTDNSDTVNPVTFGKASGLGQLVAHCSPKPITLGSIVFIDTNGNGIQEPWE
QGKQGVAVSLLFANGTLIQKQSTDSLGLFKFINPPYQNQQYIITADSVTLSIIPSPLPTS
SIPYNAATMVNGKATISNILILDPMIASSRYXLNFGVTN*kikkikklkn*

Frame A:
IIGDTIWIDSNNNSLLDNGEVGADGIKVELLVSSDLSPALDILGKPISSQVTTANGKYLF
QNVSSGPSYVVRLTIPANSTKYIAGTTYSPILTGGMSRATANGQVIVLNNFTLDTTYGSN
TWKNLNNDAGIVIPCYSIGQQSWSDGNLNGIFDINXIGFPYVNLTLFNADGSTPNDIFGK
PIQMA---

---GTGEFYNDNFRKGQIGHDDTGGLSVFQVPGFPEVASASFDATTVFEGVVKFYNNNNG
TLRSSFQVYLTDNSDTVNPVTFGKASGLGQLVAHCSPKPITLGSIVFIDTNGNGIQEPWE
QGKQGVAVSLLFANGTLIQKQSTDSLGLFKFINPPYQNQQYIITADSVTLSIIPSPLPTS
SIPYNAATMVNGKATISNILILDPMIASSRYXLNFGVTN*kikkikklkn*

Frame B:
llvilfgliqiiihy*tmvklvqmvlklny*ylqifhqhwif*vnqfqvk*qlpmvniys
kmyqvdlpm**d*lylpivpnilqvllihqf*qvvcqepqqtvk*lf*itlhwiqpmdqi
lgki*tmmqvl*shvtvlvnnhgpmvismvslismxlvshm*tlhslmqmvplqmifsan
qfkw---

---vlvnsimitlekvklvmmilvvfqfskfqvsqrlhqlhsmlqlyskv*snfititmv
pfvlhskfisliiqiqsiqlplakhrvlvnl*ptvlqnqlplvvlslliqmvmvfknhgs
kvnkvlplvyylpmvp*fknnqpih*vylnslihhtkinnillqliqlhfqlfhhlyqlh
lflitlqqwlmekqqfqiflf*iq**lqvdxi*ilvlqtkklkklkn*kik

Frame C:
yw*yyld*fk**ftirqw*swcrwy*s*iisifrsftsigyfr*tnfkssnncqw*ifip
kcikwtflcskinytcq*yqiycryylftnfdrwyvkshskrssncfe*lyigynlwiky
lekskq*crycnpmlqywstimvrw*sqwyl*yqxnwfpickpytl*crwfhsk*yfrqt
nsng---

---yw*il***l*krsnws**ywwsfsfpssrfprgcisfircyncirrcsqil*q*qwy
psffipslsh**frysqssylwqsigswstcsplfsktnypw*ycly*ykw*wysrtmga
r*trccr*fiicqwylnsktinrfirsi*ih*stipkstiyyys*fsytfnysitstnfi
ysl*rcnng*wksnnfkysyfrsndsfk*ixfkfwcyklkn*kn*kikkl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS471 (CHS471Q) /CSM/CH/CHS4-C/CHS471Q.Seq.d/                       2333   0.0
SHL451 (SHL451Q) /CSM/SH/SHL4-C/SHL451Q.Seq.d/                       1279   0.0
SHL318 (SHL318Q) /CSM/SH/SHL3-A/SHL318Q.Seq.d/                       1279   0.0
SHL194 (SHL194Q) /CSM/SH/SHL1-D/SHL194Q.Seq.d/                       1279   0.0
SHK656 (SHK656Q) /CSM/SH/SHK6-C/SHK656Q.Seq.d/                       1279   0.0
SHJ614 (SHJ614Q) /CSM/SH/SHJ6-A/SHJ614Q.Seq.d/                       1279   0.0
SHG837 (SHG837Q) /CSM/SH/SHG8-B/SHG837Q.Seq.d/                       1279   0.0
SHG543 (SHG543Q) /CSM/SH/SHG5-B/SHG543Q.Seq.d/                       1279   0.0
SHG440 (SHG440Q) /CSM/SH/SHG4-B/SHG440Q.Seq.d/                       1279   0.0
SHD578 (SHD578Q) /CSM/SH/SHD5-D/SHD578Q.Seq.d/                       1279   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   0.73   12
AC160363|AC160363.2 Bos taurus clone CH240-88I19, WORKING DRAFT SEQUENCE, 3 unordered pieces.    46   1.8    1
B76214|B76214.1 RPCI11-13P22.TV RPCI-11 Homo sapiens genomic clone RPCI-11-13P22, DNA sequence.    46   1.8    1
AC093886|AC093886.2 Homo sapiens BAC clone RP11-666L21 from 4, complete sequence.    46   1.8    1
AX144668|AX144668.1 Sequence 3390 from Patent WO0134809.           32   3.7    4
AR485304|AR485304.1 Sequence 3390 from patent US 6703492.          32   3.7    4
AF269348|AF269348.1 Staphylococcus epidermidis strain SR1 clone step.1002d10 genomic sequence.    32   3.7    4
AL808108|AL808108.3 Zebrafish DNA sequence from clone CH211-240L14.    36   4.8    5
CR441548|CR441548.1 Xenopus tropicalis EST, clone TTbA055n16 3'.    44   7.0    1
BU495682|BU495682.1 PfESToab76c04.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    44   7.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000910_1528(CP000910|pid:none) Renibacterium salmoninarum ATCC...    89   3e-16
BX248360_56(BX248360|pid:none) Corynebacterium diphtheriae gravi...    82   5e-14
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    71   9e-11
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    70   1e-10
(Q9KI14) RecName: Full=Serine-aspartate repeat-containing protei...    70   1e-10
CP000686_3542(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    70   1e-10
(Q99W46) RecName: Full=Serine-aspartate repeat-containing protei...    70   1e-10
(Q6GJA6) RecName: Full=Bone sialoprotein-binding protein;       ...    69   3e-10
AB252195_1(AB252195|pid:none) Staphylococcus aureus bbp gene for...    69   3e-10
Y18653_1(Y18653|pid:none) Staphylococcus aureus bbp gene, strain...    69   3e-10

 psg: 0.75  gvh: 0.48  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHS471 is cyt

>CHS471F.Seq
ATTATTGGTGATACTATTTGGATTGATTCAAATAATAATTCACTATTAGACAATGGTGAA
GTTGGTGCAGATGGTATTAAAGTTGAATTATTAGTATCTTCAGATCTTTCACCAGCATTG
GATATTTTAGGTAAACCAATTTCAAGTCAAGTAACAACTGCCAATGGTAAATATTTATTC
CAAAATGTATCAAGTGGACCTTCCTATGTAGTAAGATTAACTATACCTGCCAATAGTACC
AAATATATTGCAGGTACTACTTATTCACCAATTTTGACAGGTGGTATGTCAAGAGCCACA
GCAAACGGTCAAGTAATTGTTTTGAATAACTTTACATTGGATACAACCTATGGATCAAAT
ACTTGGAAAAATCTAAACAATGATGCAGGTATTGTAATCCCATGTTACAGTATTGGTCAA
CAATCATGGTCCGATGGTAATCTCAATGGTATCTTTGATATCAATGNAATTGGTTTCCCA
TATGTAAACCTTACACTCTTTAATGCAGATGGTTCCACTCCAAATGATATTTTCGGCAAA
CCAATTCAAATGGCANNNNNNNNNN

>CHS471Z.Seq
NNNNNNNNNNGGTACTGGTGAATTCTATAATGATAACTTTAGAAAAGGTCAAATTGGTCA
TGATGATACTGGTGGTCTTTCAGTTTTCCAAGTTCCAGGTTTCCCAGAGGTTGCATCAGC
TTCATTCGATGCTACAACTGTATTCGAAGGTGTAGTCAAATTTTATAACAATAACAATGG
TACCCTTCGTTCTTCATTCCAAGTTTATCTCACTGATAATTCAGATACAGTCAATCCAGT
TACCTTTGGCAAAGCATCGGGTCTTGGTCAACTTGTAGCCCACTGTTCTCCAAAACCAAT
TACCCTTGGTAGTATTGTCTTTATTGATACAAATGGTAATGGTATTCAAGAACCATGGGA
GCAAGGTAAACAAGGTGTTGCCGTTAGTTTATTATTTGCCAATGGTACCTTAATTCAAAA
ACAATCAACCGATTCATTAGGTCTATTTAAATTCATTAATCCACCATACCAAAATCAACA
ATATATTATTACAGCTGATTCAGTTACACTTTCAATTATTCCATCACCTCTACCAACTTC
ATCTATTCCTTATAACGCTGCAACAATGGTTAATGGAAAAGCAACAATTTCAAATATTCT
TATTTTAGATCCAATGATAGCTTCAAGTAGATATNATTTAAATTTTGGTGTTACAAACTA
AAAAATTAAAAAAATTAAAAAATTAAAAAATTAAA

>CHS471P.Seq
ATTATTGGTGATACTATTTGGATTGATTCAAATAATAATTCACTATTAGACAATGGTGAA
GTTGGTGCAGATGGTATTAAAGTTGAATTATTAGTATCTTCAGATCTTTCACCAGCATTG
GATATTTTAGGTAAACCAATTTCAAGTCAAGTAACAACTGCCAATGGTAAATATTTATTC
CAAAATGTATCAAGTGGACCTTCCTATGTAGTAAGATTAACTATACCTGCCAATAGTACC
AAATATATTGCAGGTACTACTTATTCACCAATTTTGACAGGTGGTATGTCAAGAGCCACA
GCAAACGGTCAAGTAATTGTTTTGAATAACTTTACATTGGATACAACCTATGGATCAAAT
ACTTGGAAAAATCTAAACAATGATGCAGGTATTGTAATCCCATGTTACAGTATTGGTCAA
CAATCATGGTCCGATGGTAATCTCAATGGTATCTTTGATATCAATGNAATTGGTTTCCCA
TATGTAAACCTTACACTCTTTAATGCAGATGGTTCCACTCCAAATGATATTTTCGGCAAA
CCAATTCAAATGGCA----------GGTACTGGTGAATTCTATAATGATAACTTTAGAAA
AGGTCAAATTGGTCATGATGATACTGGTGGTCTTTCAGTTTTCCAAGTTCCAGGTTTCCC
AGAGGTTGCATCAGCTTCATTCGATGCTACAACTGTATTCGAAGGTGTAGTCAAATTTTA
TAACAATAACAATGGTACCCTTCGTTCTTCATTCCAAGTTTATCTCACTGATAATTCAGA
TACAGTCAATCCAGTTACCTTTGGCAAAGCATCGGGTCTTGGTCAACTTGTAGCCCACTG
TTCTCCAAAACCAATTACCCTTGGTAGTATTGTCTTTATTGATACAAATGGTAATGGTAT
TCAAGAACCATGGGAGCAAGGTAAACAAGGTGTTGCCGTTAGTTTATTATTTGCCAATGG
TACCTTAATTCAAAAACAATCAACCGATTCATTAGGTCTATTTAAATTCATTAATCCACC
ATACCAAAATCAACAATATATTATTACAGCTGATTCAGTTACACTTTCAATTATTCCATC
ACCTCTACCAACTTCATCTATTCCTTATAACGCTGCAACAATGGTTAATGGAAAAGCAAC
AATTTCAAATATTCTTATTTTAGATCCAATGATAGCTTCAAGTAGATATNATTTAAATTT
TGGTGTTACAAACTAAAAAATTAAAAAAATTAAAAAATTAAAAAATTAAA