>AFH538 (AFH538Q) /CSM/AF/AFH5-B/AFH538Q.Seq.d/
XXXXXXXXXXAAGATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGT
TGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTT
GTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAA
GAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTT
TCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCC
GTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTC
TACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAG
TACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTA
CCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAA
GAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATT
CAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGANATAATGTNAATC
TAAAAAAGATTCTTCGCTAAACA

---DVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKE
TFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFY
APWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKE
NPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTXIMXI*krffak

Frame A:
---rckenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*kr
nlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*il
rsmvrslqescsnlr*ir*vlkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qr
ksnll*rskk*syelc*ihsr*rch*iqitiisn*xnvnlkkilr*t


Frame B:
---DVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKE
TFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFY
APWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKE
NPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTXIMXI*krffak


Frame C:
---m*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkk
pshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnst
lhgaviarillqstin*vst*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkk
iqslmkvkemii*tllnsfkitlplnsnyhhlklx*cxskkdssln

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH538 (AFH538Q) /CSM/AF/AFH5-B/AFH538Q.Seq.d/                       1322   0.0
AFO247 (AFO247Q) /CSM/AF/AFO2-B/AFO247Q.Seq.d/                       1300   0.0
AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/                       1300   0.0
AFI881 (AFI881Q) /CSM/AF/AFI8-D/AFI881Q.Seq.d/                       1300   0.0
AFI313 (AFI313Q) /CSM/AF/AFI3-A/AFI313Q.Seq.d/                       1300   0.0
AFI417 (AFI417Q) /CSM/AF/AFI4-A/AFI417Q.Seq.d/                       1296   0.0
AFK858 (AFK858Q) /CSM/AF/AFK8-C/AFK858Q.Seq.d/                       1292   0.0
AFH744 (AFH744Q) /CSM/AF/AFH7-B/AFH744Q.Seq.d/                       1292   0.0
AFN313 (AFN313Q) /CSM/AF/AFN3-A/AFN313Q.Seq.d/                       1291   0.0
AFH860 (AFH860Q) /CSM/AF/AFH8-C/AFH860Q.Seq.d/                       1289   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   591   e-165  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   173   5e-42
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   171   2e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   168   1e-40
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   167   3e-40
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   163   5e-39
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   160   2e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   1e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   2e-35

 psg: 0.86  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFH538 is cyt

>AFH538Z.Seq
----------AAGATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGT
TGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTT
GTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAA
GAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTT
TCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCC
GTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTC
TACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAG
TACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTA
CCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAA
GAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATT
CAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGANATAATGTNAATC
TAAAAAAGATTCTTCGCTAAACA