>CHS464 (CHS464Q) /CSM/CH/CHS4-C/CHS464Q.Seq.d/
XXXXXXXXXXTCCCACAAGCTCATGGTCCAATCATAAAGAATGGTTTACATCATTATACT
TTGGTTTTCAATTGTTTCGTTTTCCTTCAACTCTTTAATGAAATCAATGCTCGTGTACTC
GGTAGTAGAACAAATCCATTTAAAAACTTTTTCAATAACCCAATTTTCATTGCTGTCATG
ATCTTCACTTTGGGTGTTCAAATCATTTTCGTTACATTTGGTGGTTCTGCAACTTCAACC
GATTCTCTCTACATTGTTGAATGGATTTGTTGTGTTGTAGTTGGTGCCATCTCTTTACCA
GTTGGTTTATTATTAAGAAAGATTCCAATTCGTGAACCAGTTGTAAAGAATGAAATACCA
GTTCACTCTGAAGCTGTTTACACTTCTCCATCTCCAAATCCTTCCTCTTCCAATTTATTA
GGTAGTGGTGGTGCTAAACCAATCTCAAAGGATTATCCAACCTCTGGTGAATCAACTCCA
CCAATCAATGATGAAGGCTCACCATTGGTCACAAGAAAGACCTCTGTTGGTGCCAGTGCA
AATGATAATATCAATACTCCAATCCCATCATCCTCATCAAATCTTG

---PQAHGPIIKNGLHHYTLVFNCFVFLQLFNEINARVLGSRTNPFKNFFNNPIFIAVMI
FTLGVQIIFVTFGGSATSTDSLYIVEWICCVVVGAISLPVGLLLRKIPIREPVVKNEIPV
HSEAVYTSPSPNPSSSNLLGSGGAKPISKDYPTSGESTPPINDEGSPLVTRKTSVGASAN
DNINTPIPSSSSNL

Frame A:
---ptsswsnhkewftslyfgfqlfrfpstl**nqcsctr**nksi*klfq*pnfhcchd
lhfgcsnhfryiwwfcnfnrfslhc*mdllccswchlftswfiikkdsns*tscke*nts
sl*sclhfsisksflfqfir*wwc*tnlkglsnlw*instnq**rltighkkdlcwcqck
**yqysnpiiliks


Frame B:
---PQAHGPIIKNGLHHYTLVFNCFVFLQLFNEINARVLGSRTNPFKNFFNNPIFIAVMI
FTLGVQIIFVTFGGSATSTDSLYIVEWICCVVVGAISLPVGLLLRKIPIREPVVKNEIPV
HSEAVYTSPSPNPSSSNLLGSGGAKPISKDYPTSGESTPPINDEGSPLVTRKTSVGASAN
DNINTPIPSSSSNL


Frame C:
---hklmvqs*rmvyiiilwfsivsfsfnslmksmlvysvveqihlktfsitqfslls*s
slwvfksfslhlvvlqlqpilstllngfvvl*lvpslyqlvyy*erfqfvnql*rmkyqf
tlklftllhlqilplpiy*vvvvlnqsqriiqplvnqlhqsmmkahhwsqerpllvpvqm
iisilqshhphqil

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG728 (SHG728Q) /CSM/SH/SHG7-B/SHG728Q.Seq.d/                       1142   0.0
CHS464 (CHS464Q) /CSM/CH/CHS4-C/CHS464Q.Seq.d/                       1142   0.0
AHF114 (AHF114Q) /CSM/AH/AHF1-A/AHF114Q.Seq.d/                       1142   0.0
SHC348 (SHC348Q) /CSM/SH/SHC3-B/SHC348Q.Seq.d/                       1140   0.0
VFJ784 (VFJ784Q) /CSM/VF/VFJ7-D/VFJ784Q.Seq.d/                       1120   0.0
SHK225 (SHK225Q) /CSM/SH/SHK2-B/SHK225Q.Seq.d/                       1112   0.0
SHE405 (SHE405Q) /CSM/SH/SHE4-A/SHE405Q.Seq.d/                       1110   0.0
CHO511 (CHO511Q) /CSM/CH/CHO5-A/CHO511Q.Seq.d/                       1110   0.0
AHL803 (AHL803Q) /CSM/AH/AHL8-A/AHL803Q.Seq.d/                       1106   0.0
SHF533 (SHF533Q) /CSM/SH/SHF5-B/SHF533Q.Seq.d/                       1102   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X89369|X89369.1 D.discoideum mRNA for PAT1 protein.              1142   0.0    1
AC111330|AC111330.4 Rattus norvegicus clone CH230-341F17, WORKING DRAFT SEQUENCE, 2 unordered pieces.    48   0.21   1
AC133066|AC133066.3 Danio rerio clone CH211-147D21 strain Tue, WORKING DRAFT SEQUENCE, 7 ordered pieces.    46   0.82   1
AL935207|AL935207.6 Zebrafish DNA sequence from clone CH211-251J8.    46   0.82   1
BE343815|BE343815.1 EST408977 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA26D2, mRNA sequence.    44   3.2    1
CV067255| PMCA2av - Rana catesbeiana. Score = 409 bits (1052), Expect = e-113, mRNA sequence.    44   3.2    1
AC119393|AC119393.7 Rattus norvegicus clone CH230-286H14, WORKING DRAFT SEQUENCE, 1 ordered piece.    44   3.2    1
BV064073|BV064073.1 S212P6344FF8.T0 CZECHII/Ei Mus musculus STS genomic, sequence tagged site.    44   3.2    1
AC106471|AC106471.4 Rattus norvegicus clone CH230-213P16, WORKING DRAFT SEQUENCE, 1 ordered piece.    44   3.2    1
AG103247|AG103247.1 Pan troglodytes DNA, clone: PTB-106M20.F.      44   3.2    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

S57726(S57726) PAT1 protein - slime mold (Dictyostelium discoide...   285   6e-76
EU559285_1(EU559285|pid:none) Danio rerio plasma membrane calciu...    70   3e-11
BC118976_1(BC118976|pid:none) Mus musculus ATPase, Ca++ transpor...    70   5e-11
AL805924_1(AL805924|pid:none) Mouse DNA sequence from clone RP23...    70   5e-11
EU559284_1(EU559284|pid:none) Danio rerio plasma membrane calciu...    70   5e-11
AL805924_2(AL805924|pid:none) Mouse DNA sequence from clone RP23...    70   5e-11
BC089969_1(BC089969|pid:none) Rattus norvegicus ATPase, Ca++ tra...    70   5e-11
U60414_1(U60414|pid:none) Human plasma membrane calcium ATPase i...    70   5e-11
U57971_1(U57971|pid:none) Human calcium ATPase isoform 3x/a mRNA...    70   5e-11
BX248385_2(BX248385|pid:none) Zebrafish DNA sequence from clone ...    70   5e-11

 psg: 0.93  gvh: 0.71  alm: 0.18  top: 0.43  tms: 0.13  mit: 0.21  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 24.0 %: mitochondrial
	 16.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: cytoplasmic

>> prediction for CHS464 is end

>CHS464Z.Seq
NNNNNNNNNNTCCCACAAGCTCATGGTCCAATCATAAAGAATGGTTTACATCATTATACT
TTGGTTTTCAATTGTTTCGTTTTCCTTCAACTCTTTAATGAAATCAATGCTCGTGTACTC
GGTAGTAGAACAAATCCATTTAAAAACTTTTTCAATAACCCAATTTTCATTGCTGTCATG
ATCTTCACTTTGGGTGTTCAAATCATTTTCGTTACATTTGGTGGTTCTGCAACTTCAACC
GATTCTCTCTACATTGTTGAATGGATTTGTTGTGTTGTAGTTGGTGCCATCTCTTTACCA
GTTGGTTTATTATTAAGAAAGATTCCAATTCGTGAACCAGTTGTAAAGAATGAAATACCA
GTTCACTCTGAAGCTGTTTACACTTCTCCATCTCCAAATCCTTCCTCTTCCAATTTATTA
GGTAGTGGTGGTGCTAAACCAATCTCAAAGGATTATCCAACCTCTGGTGAATCAACTCCA
CCAATCAATGATGAAGGCTCACCATTGGTCACAAGAAAGACCTCTGTTGGTGCCAGTGCA
AATGATAATATCAATACTCCAATCCCATCATCCTCATCAAATCTTG