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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS448
Library CH
(Link to library)
Clone ID CHS448
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16426-1
Original site URL
Representative seq. ID CHS448P
(Link to Original site)
Representative DNA sequence 
>CHS448 (CHS448Q) /CSM/CH/CHS4-B/CHS448Q.Seq.d/
TTATCAATTGTGTGGTGATGTTAATTTTCTTTTTAATATTTTGCATTGTAAGAAGAAAAT
TCAAACAATTTTACCAATATAGATTTGAACAACACCACAAAGGTGTGTCAGTACCCCCAT
CGGATGGATTCTTTTCATGGGTTGTAGATACAATTAAAAATAGTGATAATAGTATTAAAG
ATACTGCTGGTTTGGATGGATTTATGTATTTAAGAAATGTTAAAACAAGTTTTTATATTT
GTGTTGTATTAATGGTAATATCATCAGTTATGTTATATCCAACCAATTATTATGGTAAAT
ATAATGAACATAGAGAAAAGGATGAAGATGGTAAATTACCAGATGAGGTTGTCGGGTTAA
CAATGATATCAATGGGTAATATTGGAAGXXXXXXXXXXATGATCAATTATATTTTAATTG
CTGCAGCTGGTTCATTTGGTGCAATTTTACGTATCGTTGGTTTAATTATTCAACTCATTA
AATTGAAATGGTTAGCAAAAACAAAGAGACAAATCGATGATACCCTTCATCAAGGTCCAT
TTGCTTATGGTGTAAACTATGCAAAGAATCTTTTAATCCTTCAATTGACTTTGGCTTACT
CAACTCTATCACCTTTTATTTTAATATTTGGTGCTTGGTATTTCGGTTTAAGTTATCTCG
TTCAAAAGTATAACATCATTTGGGTAAATACTCCTAATTATCAATGTGGTGGTTTCATGA
GCCCAATGCATTTCAGACGTACGATCATTGGTTTACTCATTTATCATATCCTTATGATTG
GTACTTTCAATGTTTACAAATTCTACTATGGTATTTTGGTAGTCATCTGTTTAGTTGTTA
CTATCGTATTTTGGTATTTTGCTGAATATAAATTCAAGGATATTTCAAAGAGTGGTATAA
TGGATCAATATCAAAAACGACAAACTGATGGTACCAATAGTGGTGATATTGAAATGAAAG
CCTCTGGTAGTTCAGTTATCAATGATAGTACAAGTTTAAATCCAACTGTACATCAAGAAT
TCCATGATGCTTCTCATTCAATCTCTGAATANAAAACAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
INCVVMLIFFLIFCIVRRKFKQFYQYRFEQHHKGVSVPPSDGFFSWVVDTIKNSDNSIKD
TAGLDGFMYLRNVKTSFYICVVLMVISSVMLYPTNYYGKYNEHREKDEDGKLPDEVVGLT
MISMGNIG---

---MINYILIAAAGSFGAILRIVGLIIQLIKLKWLAKTKRQIDDTLHQGPFAYGVNYAKN
LLILQLTLAYSTLSPFILIFGAWYFGLSYLVQKYNIIWVNTPNYQCGGFMSPMHFRRTII
GLLIYHILMIGTFNVYKFYYGILVVICLVVTIVFWYFAEYKFKDISKSGIMDQYQKRQTD
GTNSGDIEMKASGSSVINDSTSLNPTVHQEFHDASHSISEXKT
Translated Amino Acid sequence (All Frames) 
Frame A:
lsivw*c*fsf*yfal*eensnnftnidlnnttkvcqyphrmdsfhgl*iqlkiviivlk
illvwmdlci*emlkqvfifvly*w*yhqlcyiqpiimvnimniekrmkmvnyqmrlsg*
q*yqwvile---

---MINYILIAAAGSFGAILRIVGLIIQLIKLKWLAKTKRQIDDTLHQGPFAYGVNYAKN
LLILQLTLAYSTLSPFILIFGAWYFGLSYLVQKYNIIWVNTPNYQCGGFMSPMHFRRTII
GLLIYHILMIGTFNVYKFYYGILVVICLVVTIVFWYFAEYKFKDISKSGIMDQYQKRQTD
GTNSGDIEMKASGSSVINDSTSLNPTVHQEFHDASHSISEXKT

Frame B:
yqlcgdvnflfnilhckkkiqtilpi*i*ttpqrcvstpigwilfmgcryn*k****y*r
ycwfgwiyvfkkc*nkflylccingniisyvisnqllw*i**t*rkg*rw*itr*gcrvn
nding*ywk---

---*siif*llqlvhlvqfyvslv*lfnsln*ng*qkqrdksmipfikvhllmv*tmqri
f*sfn*lwltqlyhllf*ylvlgisv*visfksitsfg*illiinvvvs*aqcisdvrsl
vysfiisl*lvlsmftnstmvfw*ssv*lllsyfgillninsrifqrvv*winikndklm
vpivvilk*kplvvqlsmivqv*iqlyiknsmmlliqslnxkq

Frame C:
INCVVMLIFFLIFCIVRRKFKQFYQYRFEQHHKGVSVPPSDGFFSWVVDTIKNSDNSIKD
TAGLDGFMYLRNVKTSFYICVVLMVISSVMLYPTNYYGKYNEHREKDEDGKLPDEVVGLT
MISMGNIG---

---dqlyfnccswfiwcnftyrwfnysth*iemvsknketnr*ypssrsiclwcklckes
fnpsidfgllnsitfyfniwclvfrfklsrskv*hhlgkys*lsmwwfhepnafqtydhw
fthlsypydwyfqclqillwyfgshlfscyyrilvfc*i*iqgyfkewyngsiskttn*w
yq*w*y*neslw*fsyq**ykfksnctsrip*cfsfnl*ixnk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS448 (CHS448Q) /CSM/CH/CHS4-B/CHS448Q.Seq.d/                       2036   0.0
SFL440 (SFL440Q) /CSM/SF/SFL4-B/SFL440Q.Seq.d/                       1287   0.0
CHE820 (CHE820Q) /CSM/CH/CHE8-A/CHE820Q.Seq.d/                       1287   0.0
CFC669 (CFC669Q) /CSM/CF/CFC6-C/CFC669Q.Seq.d/                       1287   0.0
AHD317 (AHD317Q) /CSM/AH/AHD3-A/AHD317Q.Seq.d/                       1287   0.0
AHA204 (AHA204Q) /CSM/AH/AHA2-A/AHA204Q.Seq.d/                       1287   0.0
AFG823 (AFG823Q) /CSM/AF/AFG8-A/AFG823Q.Seq.d/                       1287   0.0
SFI453 (SFI453Q) /CSM/SF/SFI4-C/SFI453Q.Seq.d/                       1281   0.0
SFC237 (SFC237Q) /CSM/SF/SFC2-B/SFC237Q.Seq.d/                       1279   0.0
CHF487 (CHF487Q) /CSM/CH/CHF4-D/CHF487Q.Seq.d/                       1279   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.  1287   0.0    1
AF019983|AF019983.1 Dictyostelium discoideum R1062 gene, partial cds.  1227   0.0    1
AJ648912|AJ648912.1 Sus scrofa EST, clone C0003265_P22.            44   0.057  2
AC158216|AC158216.7 Mus musculus chromosome 7, clone RP24-167K8, complete sequence.    50   0.096  1
AC099719|AC099719.8 Mus musculus chromosome 7, clone RP23-394F19, complete sequence.    50   0.096  1
AC074394|AC074394.1 Homo sapiens chromosome 8 clone RP11-780J19, WORKING DRAFT SEQUENCE, 22 unordered pieces.    48   0.38   1
AC156841|AC156841.3 Bos taurus clone CH240-68M7, *** SEQUENCING IN PROGRESS ***, 19 unordered pieces.    48   0.38   1
CL887339|CL887339.1 abf83c12.x1 Soybean random, unfiltered genomic library Glycine max genomic, genomic survey sequence.    48   0.38   1
AC091194|AC091194.7 Homo sapiens chromosome 8, clone RP11-780J19, complete sequence.    48   0.38   1
AC023202|AC023202.8 Homo sapiens chromosome 8, clone RP11-203E8, complete sequence.    48   0.38   1
dna update 2005.12. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116963_57(AC116963|pid:none) Dictyostelium discoideum chromoso...   418   e-115
AF019983_1(AF019983|pid:none) Dictyostelium discoideum R1062 gen...   401   e-110
AF448811_1(AF448811|pid:none) Davidia involucrata unknown gene.        97   7e-19
AC025295_7(AC025295|pid:none) Arabidopsis thaliana chromosome 1 ...    88   4e-16
AB039928_1(AB039928|pid:none) Arabidopsis thaliana mRNA for ERD4...    88   4e-16
AK221977_1(AK221977|pid:none) Arabidopsis thaliana mRNA for ERD4...    88   5e-16
EU186363_1(EU186363|pid:none) Brassica rapa clone S27 early-resp...    84   6e-15
CR380953_357(CR380953|pid:none) Candida glabrata strain CBS138 c...    74   6e-12
CR382124_759(CR382124|pid:none) Kluyveromyces lactis strain NRRL...    73   2e-11
AE016820_257(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...    70   9e-11
protein update 2009. 4.13
PSORT 

 psg: 1.03  gvh: 0.57  alm: 0.15  top: 0.77  tms: 0.40  mit: 0.36  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 40.0 %: plasma membrane
	 32.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: cytoplasmic
	  4.0 %: nuclear

>> prediction for CHS448 is pla
5' end seq. ID CHS448F
5' end seq. 
>CHS448F.Seq
TTATCAATTGTGTGGTGATGTTAATTTTCTTTTTAATATTTTGCATTGTAAGAAGAAAAT
TCAAACAATTTTACCAATATAGATTTGAACAACACCACAAAGGTGTGTCAGTACCCCCAT
CGGATGGATTCTTTTCATGGGTTGTAGATACAATTAAAAATAGTGATAATAGTATTAAAG
ATACTGCTGGTTTGGATGGATTTATGTATTTAAGAAATGTTAAAACAAGTTTTTATATTT
GTGTTGTATTAATGGTAATATCATCAGTTATGTTATATCCAACCAATTATTATGGTAAAT
ATAATGAACATAGAGAAAAGGATGAAGATGGTAAATTACCAGATGAGGTTGTCGGGTTAA
CAATGATATCAATGGGTAATATTGGAAGNNNNNNNNNN
Length of 5' end seq. 398
3' end seq. ID CHS448Z
3' end seq. 
>CHS448Z.Seq
NNNNNNNNNNATGATCAATTATATTTTAATTGCTGCAGCTGGTTCATTTGGTGCAATTTT
ACGTATCGTTGGTTTAATTATTCAACTCATTAAATTGAAATGGTTAGCAAAAACAAAGAG
ACAAATCGATGATACCCTTCATCAAGGTCCATTTGCTTATGGTGTAAACTATGCAAAGAA
TCTTTTAATCCTTCAATTGACTTTGGCTTACTCAACTCTATCACCTTTTATTTTAATATT
TGGTGCTTGGTATTTCGGTTTAAGTTATCTCGTTCAAAAGTATAACATCATTTGGGTAAA
TACTCCTAATTATCAATGTGGTGGTTTCATGAGCCCAATGCATTTCAGACGTACGATCAT
TGGTTTACTCATTTATCATATCCTTATGATTGGTACTTTCAATGTTTACAAATTCTACTA
TGGTATTTTGGTAGTCATCTGTTTAGTTGTTACTATCGTATTTTGGTATTTTGCTGAATA
TAAATTCAAGGATATTTCAAAGAGTGGTATAATGGATCAATATCAAAAACGACAAACTGA
TGGTACCAATAGTGGTGATATTGAAATGAAAGCCTCTGGTAGTTCAGTTATCAATGATAG
TACAAGTTTAAATCCAACTGTACATCAAGAATTCCATGATGCTTCTCATTCAATCTCTGA
ATANAAAACAAA
Length of 3' end seq. 672
Connected seq. ID CHS448P
Connected seq. 
>CHS448P.Seq
TTATCAATTGTGTGGTGATGTTAATTTTCTTTTTAATATTTTGCATTGTAAGAAGAAAAT
TCAAACAATTTTACCAATATAGATTTGAACAACACCACAAAGGTGTGTCAGTACCCCCAT
CGGATGGATTCTTTTCATGGGTTGTAGATACAATTAAAAATAGTGATAATAGTATTAAAG
ATACTGCTGGTTTGGATGGATTTATGTATTTAAGAAATGTTAAAACAAGTTTTTATATTT
GTGTTGTATTAATGGTAATATCATCAGTTATGTTATATCCAACCAATTATTATGGTAAAT
ATAATGAACATAGAGAAAAGGATGAAGATGGTAAATTACCAGATGAGGTTGTCGGGTTAA
CAATGATATCAATGGGTAATATTGGAAG----------ATGATCAATTATATTTTAATTG
CTGCAGCTGGTTCATTTGGTGCAATTTTACGTATCGTTGGTTTAATTATTCAACTCATTA
AATTGAAATGGTTAGCAAAAACAAAGAGACAAATCGATGATACCCTTCATCAAGGTCCAT
TTGCTTATGGTGTAAACTATGCAAAGAATCTTTTAATCCTTCAATTGACTTTGGCTTACT
CAACTCTATCACCTTTTATTTTAATATTTGGTGCTTGGTATTTCGGTTTAAGTTATCTCG
TTCAAAAGTATAACATCATTTGGGTAAATACTCCTAATTATCAATGTGGTGGTTTCATGA
GCCCAATGCATTTCAGACGTACGATCATTGGTTTACTCATTTATCATATCCTTATGATTG
GTACTTTCAATGTTTACAAATTCTACTATGGTATTTTGGTAGTCATCTGTTTAGTTGTTA
CTATCGTATTTTGGTATTTTGCTGAATATAAATTCAAGGATATTTCAAAGAGTGGTATAA
TGGATCAATATCAAAAACGACAAACTGATGGTACCAATAGTGGTGATATTGAAATGAAAG
CCTCTGGTAGTTCAGTTATCAATGATAGTACAAGTTTAAATCCAACTGTACATCAAGAAT
TCCATGATGCTTCTCATTCAATCTCTGAATANAAAACAAA
Length of connected seq. 1050
Full length Seq ID -
Full length Seq. -
Length of full length seq. -