>CHS446 (CHS446Q) /CSM/CH/CHS4-B/CHS446Q.Seq.d/
GTATGATTATTATAATTCAAATTAAAAATATATTATATAAATATATATAAATATATATTA
AATAAAAATAATAATAAAATAAATTTAATATAATAAAAAATTTTTTTGAGAAAAAAAAAA
ANTAAAAAAAAAAAAAAAAAAAAAANCCCNAAAAANCCCNAAAAAAAANCCCCNNNAAAA
AAAXXXXXXXXXXTAGCTCAAGATTCTCATTGTGATGATGGTAATCCATGTACTGATAAT
GCTTGTGTCGCTTCAGCTAATAGTACCGCCAATAGCCAAGTTGGTCAATGCACTCAAACT
CCAACTGCTAAATGTGATTTGGGTGATAAATGTATGGTTTACAGTTGTACCTTGGAAGGT
GGTTGTAATGCAACTCAACGTGTTTGTCCAAACTCTGGTGCTTGTAAAATTGGTTATTGT
GAACCTGGTTTCGGTTGTAAATTAAAGGATAGAGTTTGTTCTTCCGATTGGTATTGTATT
GAAGCTCAATGTGATGCAAAATATGGTTGCATTCAATTCGATAGAAGATGTGCTCCTGAT
AATAGTAAATGTCAAGAAGGTGTCTGTGTAAACAATACTGCTATCGGTAAAAATGATGGA
AAATGTATTTCTGAAGATTATGATCCAAAACCATTTGTTTGTCAAAAAGCTGCTTTAGTT
TCAACTGCTGTTATTGCAAGTGTTGTTGTAGTTGGTGCTGTTGTTTTAGGTGCTGCTATA
TTTGCGGGTA

v*ll*fklkiyyiniykyilnknnnkinli**KIFLRKKKXKKKKKKKXPKXPXKKXPXK
K---

---AQDSHCDDGNPCTDNACVASANSTANSQVGQCTQTPTAKCDLGDKCMVYSCTLEGGC
NATQRVCPNSGACKIGYCEPGFGCKLKDRVCSSDWYCIEAQCDAKYGCIQFDRRCAPDNS
KCQEGVCVNNTAIGKNDGKCISEDYDPKPFVCQKAALVSTAVIASVVVVGAVVLGAAIFA
G

Frame A:
v*ll*fklkiyyiniykyilnknnnkinli**KIFLRKKKXKKKKKKKXPKXPXKKXPXK
K---

---*lkilivmmvihvlimlvslqlivppiaklvnalklqllnviwvinvwftvvpwkvv
vmqlnvfvqtlvlvklvivnlvsvvn*riefvlpigivlklnvmqnmvafnsiedvllii
vnvkkvsv*tillsvkmmenvflkimiqnhlfvkkll*fqllllqvll*lvllf*vllyl
rv

Frame B:
ydyynsn*kyii*iyiniy*ikiiik*i*ynkkff*ekkkxkkkkkkkxxkxpkkxpxxk
---

---ssrfsl**w*smy**clcrfs**yrq*pswsmhsnsnc*m*fg**myglqlylgrwl
*cnstclsklwcl*nwll*twfrl*ikg*slffrlvly*ssm*ckiwlhsir*kmcs***
*msrrclckqycyr*k*wkmyf*rl*skticlskscfsfnccyckcccswcccfrccyic
g

Frame C:
miiiiqiknilykyi*iyik*k***nkfniiknffekkkx*kkkkkkxpxkxxkkxpxkk
---

---AQDSHCDDGNPCTDNACVASANSTANSQVGQCTQTPTAKCDLGDKCMVYSCTLEGGC
NATQRVCPNSGACKIGYCEPGFGCKLKDRVCSSDWYCIEAQCDAKYGCIQFDRRCAPDNS
KCQEGVCVNNTAIGKNDGKCISEDYDPKPFVCQKAALVSTAVIASVVVVGAVVLGAAIFA
G

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHQ874 (CHQ874Q) /CSM/CH/CHQ8-D/CHQ874Q.Seq.d/                       1065   0.0
SSD152 (SSD152Q) /CSM/SS/SSD1-C/SSD152Q.Seq.d/                       1065   0.0
SHL876 (SHL876Q) /CSM/SH/SHL8-D/SHL876Q.Seq.d/                       1065   0.0
SHL603 (SHL603Q) /CSM/SH/SHL6-A/SHL603Q.Seq.d/                       1065   0.0
SHL161 (SHL161Q) /CSM/SH/SHL1-C/SHL161Q.Seq.d/                       1065   0.0
SHK812 (SHK812Q) /CSM/SH/SHK8-A/SHK812Q.Seq.d/                       1065   0.0
SHK734 (SHK734Q) /CSM/SH/SHK7-B/SHK734Q.Seq.d/                       1065   0.0
SHK126 (SHK126Q) /CSM/SH/SHK1-B/SHK126Q.Seq.d/                       1065   0.0
SHJ815 (SHJ815Q) /CSM/SH/SHJ8-A/SHJ815Q.Seq.d/                       1065   0.0
SHG811 (SHG811Q) /CSM/SH/SHG8-A/SHG811Q.Seq.d/                       1065   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    66   1e-06  1
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    42   0.003  4
CX053834|CX053834.1 UCRCS09_8E08_g Ruby Orange Developing Seed cDNA Library UCRCS09 Citrus sinensis cDNA clone UCRCS09-8E08-I15-1-5.g, mRNA sequence.    50   0.066  1
CC946427|CC946427.1 BOIFV20TF BO_1.4_1.6_KB_nuc Brassica oleracea genomic clone BOIFV20, genomic survey sequence.    50   0.066  1
CC075688|CC075688.1 CSU-K33r.25G4.SP6 CSU-K33r Aedes aegypti genomic clone CSU-K33r.25G4, DNA sequence.    50   0.066  1
BH960705|BH960705.1 odd88f07.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    50   0.066  1
AR549621|AR549621.1 Sequence 4752 from patent US 6747137.          46   0.29   2
AL287678|AL287678.1 Tetraodon nigroviridis genome survey sequence T7 end of clone 105D19 of library G from Tetraodon nigroviridis.    38   0.87   2
CX118034|CX118034.1 EI112L19 A normalized whole-life-cycle cDNA library of rice Oryza sativa (indica cultivar-group) cDNA clone EI112L19 5', mRNA sequence.    46   1.0    1
CB652801|CB652801.1 OSJNEc03D04.f OSJNEc Oryza sativa (japonica cultivar-group) cDNA clone OSJNEc03D04 5', mRNA sequence.    46   1.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                341   1e-92
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   150   3e-35
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   145   2e-33
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   138   2e-31
(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                125   1e-27
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                107   5e-22
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                 97   4e-19
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    72   2e-11
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...    68   2e-10
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    66   1e-09

 psg: 0.21  gvh: 0.50  alm: 0.16  top: 0.60  tms: 0.07  mit: 0.36  mip: 0.02
 nuc: 0.20  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: plasma membrane
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for CHS446 is cyt

>CHS446F.Seq
GTATGATTATTATAATTCAAATTAAAAATATATTATATAAATATATATAAATATATATTA
AATAAAAATAATAATAAAATAAATTTAATATAATAAAAAATTTTTTTGAGAAAAAAAAAA
ANTAAAAAAAAAAAAAAAAAAAAAANCCCNAAAAANCCCNAAAAAAAANCCCCNNNAAAA
AAANNNNNNNNNN

>CHS446Z.Seq
NNNNNNNNNNTAGCTCAAGATTCTCATTGTGATGATGGTAATCCATGTACTGATAATGCT
TGTGTCGCTTCAGCTAATAGTACCGCCAATAGCCAAGTTGGTCAATGCACTCAAACTCCA
ACTGCTAAATGTGATTTGGGTGATAAATGTATGGTTTACAGTTGTACCTTGGAAGGTGGT
TGTAATGCAACTCAACGTGTTTGTCCAAACTCTGGTGCTTGTAAAATTGGTTATTGTGAA
CCTGGTTTCGGTTGTAAATTAAAGGATAGAGTTTGTTCTTCCGATTGGTATTGTATTGAA
GCTCAATGTGATGCAAAATATGGTTGCATTCAATTCGATAGAAGATGTGCTCCTGATAAT
AGTAAATGTCAAGAAGGTGTCTGTGTAAACAATACTGCTATCGGTAAAAATGATGGAAAA
TGTATTTCTGAAGATTATGATCCAAAACCATTTGTTTGTCAAAAAGCTGCTTTAGTTTCA
ACTGCTGTTATTGCAAGTGTTGTTGTAGTTGGTGCTGTTGTTTTAGGTGCTGCTATATTT
GCGGGTA

>CHS446P.Seq
GTATGATTATTATAATTCAAATTAAAAATATATTATATAAATATATATAAATATATATTA
AATAAAAATAATAATAAAATAAATTTAATATAATAAAAAATTTTTTTGAGAAAAAAAAAA
ANTAAAAAAAAAAAAAAAAAAAAAANCCCNAAAAANCCCNAAAAAAAANCCCCNNNAAAA
AAA----------TAGCTCAAGATTCTCATTGTGATGATGGTAATCCATGTACTGATAAT
GCTTGTGTCGCTTCAGCTAATAGTACCGCCAATAGCCAAGTTGGTCAATGCACTCAAACT
CCAACTGCTAAATGTGATTTGGGTGATAAATGTATGGTTTACAGTTGTACCTTGGAAGGT
GGTTGTAATGCAACTCAACGTGTTTGTCCAAACTCTGGTGCTTGTAAAATTGGTTATTGT
GAACCTGGTTTCGGTTGTAAATTAAAGGATAGAGTTTGTTCTTCCGATTGGTATTGTATT
GAAGCTCAATGTGATGCAAAATATGGTTGCATTCAATTCGATAGAAGATGTGCTCCTGAT
AATAGTAAATGTCAAGAAGGTGTCTGTGTAAACAATACTGCTATCGGTAAAAATGATGGA
AAATGTATTTCTGAAGATTATGATCCAAAACCATTTGTTTGTCAAAAAGCTGCTTTAGTT
TCAACTGCTGTTATTGCAAGTGTTGTTGTAGTTGGTGCTGTTGTTTTAGGTGCTGCTATA
TTTGCGGGTA