>CHS443 (CHS443Q) /CSM/CH/CHS4-B/CHS443Q.Seq.d/
TTGAACCAGTTGATGCAAGATTATCATTTCCATGTTTTGATGAACCATCATTGAAAGCCA
ATTGGACAATTTGGATTACACATCCAAATAATTATAAAGCACTCTCAAATATGCCAGCCT
ATTTAGTGGAAGATAATAAGGTAGCCCATAAAACAACCACTCGATTCGATACTACCCCAA
AGATGAGTAGTTATTTGGTTTGTATTGTAGTTCATCAATTTAGTAGTAAATCCGATTTCA
TTGACCGTAGAGGTAAAGAAGGTGCAACCTCTGTTCCATTGACAGTTTGGGCCGCCGATC
ATTTAATGGATACCGTTGATTTTTCATTGGATATGGCAAAGAAATCATTTGTATTCTTTG
AAGATTATTTTGATATTCTTTATCCATTACCAAAGATGGATCTTGTAGCTATACCTGATT
TCGCTGCGGGTGCAATGGAGAATTTTGGTTTAATGACATTTAGAGAATTCCGATCTACTA
TAANAGTAATAAAACTTCAGATCAAGAGAATAAACAAAGAGTGGCAGAAGTGGTTTCACA
TGAGATTGCTCATCAATGGTTTGGTGATTTGGTCACAATGAAATGGTGGAATGATCTTTG
GTTAXXXXXXXXXXCTTTCATTTGTTCACAATCGTTTAGAAACCGTTGTTCCATGTTATA
TTCAATACATTAAAAAAGCTCAATACATTTTCAATAAAGGCTTATTACCACTCATCAAAC
TTAATATTACTTCGGATGATGAACTCTCCCTATAATCAATTGGAATTAAGAAAATCTGCC
TTTTCAAAAGTAAACTCCTTATCAATTACTTCTTTAAGAATTCATCTTAATCAAATTTAT
TTAGATAATATTGATACACCAGAAAATATTGATCCATCAATTAGAGCTCCATTATTCTCA
TCAATTATACAAAATGGTGATATTGAAGAATATGAATGGGTAGTTAATAGATTTGAAAAT
ACAAATGCAATCAATGAAAGAATCGATGCTTTGAAAGCAATTTCCAATCCAAAGAATCCA
CAATTAATTAGAAATACTTTAAAAATGCTTTTAGATGGTAAAATTAAAAGTCAAGACTTT
TATATGGTTTTCTTGGAAATGTCATATTCACCATTTGCAAGAGAATTAGCTTGGAACTTT
TTATTAGATAATTTTAATTTCATTTCAGAGAATTCAACACCAGGTGATATTGGTAAATAT
GTAACCTATTTTGCATCATCAATGGATTCCCAAGCAAAAATTGATCAAATTAAACAATTT
TTCACAGATGCTCATCCAATCCCAGCCTCTTCGTTGGCAAATGCTATATCAAGTATTCAA
TACAATANAATGGTTAAAAAACCAAGCACCNAACTTGNATTTTTTAAATAAANA

*ts*ckiiismf**tiiesqldnldytsk*l*stlkyaslfsgr**gsp*nnhsiryypk
de*lfglycsssi***irfh*p*r*rrcnlcsidslgrrsfngyr*ffigygkeiicil*
rlf*yslsitkdgscsyt*frcgcngefwfndi*RIPIYYXSNKTSDQENKQRVAEVVSH
EIAHQWFGDLVTMKWWNDLWL---

---LSFVHNRLETVVPCYIQYIKKAQYIFNKGLLPLIKLNITSDDELSL*sigikkiclf
kskllinyffknss*snlfr*y*ytrky*sin*ssiilinytkw*y*ri*mgs**i*kyk
cnq*knrcfesnfqskestin*kyfknafrw*n*ksrllygflgnviftickrislelfi
r*f*fhfrefntr*yw*icnlfciingfpskn*sn*tifhrcssnpslfvgkcyikysiq
xng*ktkhxtxif*ix

Frame A:
lnqlmqdyhfhvlmnhh*kpigqfglhiqiiikhsqicqpi*wkiir*pikqpldsilpq
r*vviwfvl*finlvvnpisltvevkkvqplfh*qfgppii*wiplifhwiwqrnhlysl
kiiliffihyqrwil*lylislrvqwrilv**hlensdll*x**nfrsre*tksgrsgft
*dcssmvw*fghnemve*slv---

---LSFVHNRLETVVPCYIQYIKKAQYIFNKGLLPLIKLNITSDDELSL*sigikkiclf
kskllinyffknss*snlfr*y*ytrky*sin*ssiilinytkw*y*ri*mgs**i*kyk
cnq*knrcfesnfqskestin*kyfknafrw*n*ksrllygflgnviftickrislelfi
r*f*fhfrefntr*yw*icnlfciingfpskn*sn*tifhrcssnpslfvgkcyikysiq
xng*ktkhxtxif*ix

Frame B:
*ts*ckiiismf**tiiesqldnldytsk*l*stlkyaslfsgr**gsp*nnhsiryypk
de*lfglycsssi***irfh*p*r*rrcnlcsidslgrrsfngyr*ffigygkeiicil*
rlf*yslsitkdgscsyt*frcgcngefwfndi*RIPIYYXSNKTSDQENKQRVAEVVSH
EIAHQWFGDLVTMKWWNDLWL---

---fhlftiv*kplfhvifntlkklntfsikayyhssnlillrmmnspynqlelrksafs
kvnslsitslrihlnqiyldnidtpenidpsiraplfssiiqngdieeyewvvnrfentn
aineridalkaisnpknpqlirntlkmlldgkiksqdfymvflemsyspfarelawnfll
dnfnfisenstpgdigkyvtyfassmdsqakidqikqfftdahpipasslanaissiqyn
xmvkkpstxlxffk*x

Frame C:
epvdarlsfpcfdepslkanwtiwithpnnykalsnmpaylvednkvahktttrfdttpk
mssylvcivvhqfssksdfidrrgkegatsvpltvwaadhlmdtvdfsldmakksfvffe
dyfdilyplpkmdlvaipdfaagamenfglmtfrefrstixviklqikrinkewqkwfhm
rllinglviwsq*nggmifg---

---ficsqsfrnrcsmlysih*kssihfq*rlitthqt*yyfg**tlpiinwn*enlpfq
k*tpyqlll*efilikfi*iilihqkilihqlelhyshqlykmvilknmng*lidlkiqm
qsmkesml*kqfpiqrihn*leil*kcf*mvklkvktfiwfswkchihhlqen*lgtfy*
iilisfqriqhqvilvnm*pilhhqwipkqkliklnnfsqmliqsqplrwqmlyqvfnti
xwlknqapnlxflnk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS443 (CHS443Q) /CSM/CH/CHS4-B/CHS443Q.Seq.d/                       2637   0.0
SHD666 (SHD666Q) /CSM/SH/SHD6-C/SHD666Q.Seq.d/                       1483   0.0
CHI763 (CHI763Q) /CSM/CH/CHI7-C/CHI763Q.Seq.d/                       1481   0.0
CHQ511 (CHQ511Q) /CSM/CH/CHQ5-A/CHQ511Q.Seq.d/                       1475   0.0
AFN664 (AFN664Q) /CSM/AF/AFN6-C/AFN664Q.Seq.d/                       1457   0.0
SFG193 (SFG193Q) /CSM/SF/SFG1-D/SFG193Q.Seq.d/                       1431   0.0
CFF794 (CFF794Q) /CSM/CF/CFF7-D/CFF794Q.Seq.d/                       1429   0.0
AHD842 (AHD842Q) /CSM/AH/AHD8-B/AHD842Q.Seq.d/                       1421   0.0
AHK294 (AHK294Q) /CSM/AH/AHK2-D/AHK294Q.Seq.d/                       1407   0.0
CFK367 (CFK367Q) /CSM/CF/CFK3-C/CFK367Q.Seq.d/                       1388   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.   827   0.0    11
AJ866928|AJ866928.1 Lactobacillus sanfranciscensis oppA gene and partial pepN gene.    78   6e-10  1
AZ534498|AZ534498.1 ENTDI94TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    74   5e-09  2
AZ670291|AZ670291.1 ENTIG51TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    74   9e-09  1
AY555583|AY555583.1 Pediculus humanus glutamyl aminopeptidase (APA1) mRNA, complete cds.    56   6e-08  3
CA302966|CA302966.1 taa01d07.y1 Hydra cDNA library Hydra magnipapillata cDNA 5' similar to TR:Q9UEM2 Q9UEM2 PUROMYCIN SENSITIVE AMINOPEPTIDASE ;, mRNA sequence.    64   6e-07  2
DN815315| Puromycin-sensitive aminopeptidase [Mus musculus], mRNA sequence.    64   8e-07  2
Z69794|Z69794.1 Caenorhabditis elegans Cosmid R03G8.               64   8e-07  3
BP505874|BP505874.1 Hydra magnipapillata cDNA, clone:hm_03903.     64   8e-07  2
BH149559|BH149559.1 ENTPM86TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    66   2e-06  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR559943_8(CR559943|pid:none) Zebrafish DNA sequence from clone ...   150   6e-48
AM888281_1(AM888281|pid:none) Homo sapiens ENPEP gene for glutam...   144   2e-45
AM888278_1(AM888278|pid:none) Homo sapiens ENPEP gene for glutam...   144   2e-45
AM888276_1(AM888276|pid:none) Homo sapiens ENPEP gene for glutam...   144   2e-45
AK313412_1(AK313412|pid:none) Homo sapiens cDNA, FLJ93950, highl...   144   2e-45
AM888283_1(AM888283|pid:none) Homo sapiens ENPEP gene for glutam...   144   2e-45
AM888280_1(AM888280|pid:none) Homo sapiens ENPEP gene for glutam...   144   2e-45
(Q07075) RecName: Full=Glutamyl aminopeptidase;          EC=3.4....   144   2e-45
(Q95334) RecName: Full=Glutamyl aminopeptidase;          EC=3.4....   144   3e-45
CR860183_1(CR860183|pid:none) Pongo abelii mRNA; cDNA DKFZp469K1...   144   4e-45

 psg: 0.37  gvh: 0.53  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for CHS443 is nuc

>CHS443F.Seq
TTGAACCAGTTGATGCAAGATTATCATTTCCATGTTTTGATGAACCATCATTGAAAGCCA
ATTGGACAATTTGGATTACACATCCAAATAATTATAAAGCACTCTCAAATATGCCAGCCT
ATTTAGTGGAAGATAATAAGGTAGCCCATAAAACAACCACTCGATTCGATACTACCCCAA
AGATGAGTAGTTATTTGGTTTGTATTGTAGTTCATCAATTTAGTAGTAAATCCGATTTCA
TTGACCGTAGAGGTAAAGAAGGTGCAACCTCTGTTCCATTGACAGTTTGGGCCGCCGATC
ATTTAATGGATACCGTTGATTTTTCATTGGATATGGCAAAGAAATCATTTGTATTCTTTG
AAGATTATTTTGATATTCTTTATCCATTACCAAAGATGGATCTTGTAGCTATACCTGATT
TCGCTGCGGGTGCAATGGAGAATTTTGGTTTAATGACATTTAGAGAATTCCGATCTACTA
TAANAGTAATAAAACTTCAGATCAAGAGAATAAACAAAGAGTGGCAGAAGTGGTTTCACA
TGAGATTGCTCATCAATGGTTTGGTGATTTGGTCACAATGAAATGGTGGAATGATCTTTG
GTTANNNNNNNNNN

>CHS443Z.Seq
NNNNNNNNNNCTTTCATTTGTTCACAATCGTTTAGAAACCGTTGTTCCATGTTATATTCA
ATACATTAAAAAAGCTCAATACATTTTCAATAAAGGCTTATTACCACTCATCAAACTTAA
TATTACTTCGGATGATGAACTCTCCCTATAATCAATTGGAATTAAGAAAATCTGCCTTTT
CAAAAGTAAACTCCTTATCAATTACTTCTTTAAGAATTCATCTTAATCAAATTTATTTAG
ATAATATTGATACACCAGAAAATATTGATCCATCAATTAGAGCTCCATTATTCTCATCAA
TTATACAAAATGGTGATATTGAAGAATATGAATGGGTAGTTAATAGATTTGAAAATACAA
ATGCAATCAATGAAAGAATCGATGCTTTGAAAGCAATTTCCAATCCAAAGAATCCACAAT
TAATTAGAAATACTTTAAAAATGCTTTTAGATGGTAAAATTAAAAGTCAAGACTTTTATA
TGGTTTTCTTGGAAATGTCATATTCACCATTTGCAAGAGAATTAGCTTGGAACTTTTTAT
TAGATAATTTTAATTTCATTTCAGAGAATTCAACACCAGGTGATATTGGTAAATATGTAA
CCTATTTTGCATCATCAATGGATTCCCAAGCAAAAATTGATCAAATTAAACAATTTTTCA
CAGATGCTCATCCAATCCCAGCCTCTTCGTTGGCAAATGCTATATCAAGTATTCAATACA
ATANAATGGTTAAAAAACCAAGCACCNAACTTGNATTTTTTAAATAAANA

>CHS443P.Seq
TTGAACCAGTTGATGCAAGATTATCATTTCCATGTTTTGATGAACCATCATTGAAAGCCA
ATTGGACAATTTGGATTACACATCCAAATAATTATAAAGCACTCTCAAATATGCCAGCCT
ATTTAGTGGAAGATAATAAGGTAGCCCATAAAACAACCACTCGATTCGATACTACCCCAA
AGATGAGTAGTTATTTGGTTTGTATTGTAGTTCATCAATTTAGTAGTAAATCCGATTTCA
TTGACCGTAGAGGTAAAGAAGGTGCAACCTCTGTTCCATTGACAGTTTGGGCCGCCGATC
ATTTAATGGATACCGTTGATTTTTCATTGGATATGGCAAAGAAATCATTTGTATTCTTTG
AAGATTATTTTGATATTCTTTATCCATTACCAAAGATGGATCTTGTAGCTATACCTGATT
TCGCTGCGGGTGCAATGGAGAATTTTGGTTTAATGACATTTAGAGAATTCCGATCTACTA
TAANAGTAATAAAACTTCAGATCAAGAGAATAAACAAAGAGTGGCAGAAGTGGTTTCACA
TGAGATTGCTCATCAATGGTTTGGTGATTTGGTCACAATGAAATGGTGGAATGATCTTTG
GTTA----------CTTTCATTTGTTCACAATCGTTTAGAAACCGTTGTTCCATGTTATA
TTCAATACATTAAAAAAGCTCAATACATTTTCAATAAAGGCTTATTACCACTCATCAAAC
TTAATATTACTTCGGATGATGAACTCTCCCTATAATCAATTGGAATTAAGAAAATCTGCC
TTTTCAAAAGTAAACTCCTTATCAATTACTTCTTTAAGAATTCATCTTAATCAAATTTAT
TTAGATAATATTGATACACCAGAAAATATTGATCCATCAATTAGAGCTCCATTATTCTCA
TCAATTATACAAAATGGTGATATTGAAGAATATGAATGGGTAGTTAATAGATTTGAAAAT
ACAAATGCAATCAATGAAAGAATCGATGCTTTGAAAGCAATTTCCAATCCAAAGAATCCA
CAATTAATTAGAAATACTTTAAAAATGCTTTTAGATGGTAAAATTAAAAGTCAAGACTTT
TATATGGTTTTCTTGGAAATGTCATATTCACCATTTGCAAGAGAATTAGCTTGGAACTTT
TTATTAGATAATTTTAATTTCATTTCAGAGAATTCAACACCAGGTGATATTGGTAAATAT
GTAACCTATTTTGCATCATCAATGGATTCCCAAGCAAAAATTGATCAAATTAAACAATTT
TTCACAGATGCTCATCCAATCCCAGCCTCTTCGTTGGCAAATGCTATATCAAGTATTCAA
TACAATANAATGGTTAAAAAACCAAGCACCNAACTTGNATTTTTTAAATAAANA