>CHS425 (CHS425Q) /CSM/CH/CHS4-B/CHS425Q.Seq.d/
AACATACTCCAGTAAAAAATGGATCTTTATCAAGCTTTGGCCAATGCAAATCATCAATCT
GAAAGTGTTCGTTCAGAAGCCGAATCATTTTTAACCAATGCATCACAACAAAACTTTCCA
TTATTTATTCACTCATTAACATTAGAACTTACAAATGAAGAGAGAGATGTACAAATTCGT
CAATTAGCAGGTATTGTTTTAAAGAATAGTATTCACTCTAAAAATAGCGAAAGAAATGAA
ATTTTAATTAAACAATGGGCATCAATTGAACCAAATGCTAGAAACGTCATTAAAAATACT
CTTTTACAAGGTTTAGGATCACCAAAATATGAAGCACGTCACACTGTAGCTATTGTAATT
TCACGTATTGGTCTTATTGAAATTCCATACGCAATGTGGGAAGAATTAATCCCAGCATTA
TTTAAAAACATTGAAAGTGGTTCAGAAGGTATTAAACAATCTACTCTTCAAACACTTGGT
TATATTTGTGAAGAAATTGATCCAAATGTTATGTCAAAATATTCAGATAATGTATTAAGA
GTTATTACAGATGGTATCAAAGATGAATCACAAAATGTAAAATTAGCAGGTATTCAAGCA
TTATGCTTTGCATTAGAATTTGTAAAGAAAAATTTTGATATTAAAGAXXXXXXXXXXTTA
TTCAACGTATTCAAGAATCAATCAATCATCATCTACGAAGAGGCTCTCCTTGCCATTGGT
GCCATCATCTTGGCTTTGGATGCTGATTTCGAACCTTACTTTTCAAATTTCCTTCCAATC
CTTGTAAACTTCCTCAAGAATGTCGATTTTGGTTCCGTANCAAACATTGCAATTGGTATC
GTTGGTGATTTAGCTCGTGCTTTTGGTAAGAAATTCTCTGGTGTTTGCGCTGAAATCGTT
CCATTGATCGTTAGTGATCTCACCAATCCAAAACTCTCTATGAATGCTAAACCAAGTGCT
ATCAGTTGTCTCTGTGATATCGCCATTAGTGTTGGTGCCGATTTCATTCCTTTCCTTCCA
ATGGTTATGCCAATTCTTCATCAAGCCTCTACCACTGAAATCGATGATGAAGACTTTTTA
AATGAACTCCGTGAAACTATCTTCCAAACTTACACTGGTATCATTCAAGGTCTCAAGAGT
GATAATCAAATCGAAGAACTCAATCAATACATCAATCCAATGTTAGACTTTGTTGTACTT
ATAAATGATGATCCAGAACGTTATGATTCTGTTACTTCTGCTGCCTTGGCCTTACTTGGT
GATTTAGCTCAAGCTATGGGTGATAGTATTAGAAATCTTTTAAACAACAAAATAATTAGA
GAATTTGTAAACAATGGTATTCAAAAAGGTATACCATATGCGCATTATGCNATATTATCA
ATTTTTCCAAAAAAACATTAATT

nilq*KMDLYQALANANHQSESVRSEAESFLTNASQQNFPLFIHSLTLELTNEERDVQIR
QLAGIVLKNSIHSKNSERNEILIKQWASIEPNARNVIKNTLLQGLGSPKYEARHTVAIVI
SRIGLIEIPYAMWEELIPALFKNIESGSEGIKQSTLQTLGYICEEIDPNVMSKYSDNVLR
VITDGIKDESQNVKLAGIQALCFALEFVKKNFDIK---

---LFNVFKNQSIIIYEEALLAIGAIILALDADFEPYFSNFLPILVNFLKNVDFGSVXNI
AIGIVGDLARAFGKKFSGVCAEIVPLIVSDLTNPKLSMNAKPSAISCLCDIAISVGADFI
PFLPMVMPILHQASTTEIDDEDFLNELRETIFQTYTGIIQGLKSDNQIEELNQYINPMLD
FVVLINDDPERYDSVTSAALALLGDLAQAMGDSIRNLLNNKIIREFVNNGIQKGIPYAHY
AILSIFPKKH*

Frame A:
nilq*KMDLYQALANANHQSESVRSEAESFLTNASQQNFPLFIHSLTLELTNEERDVQIR
QLAGIVLKNSIHSKNSERNEILIKQWASIEPNARNVIKNTLLQGLGSPKYEARHTVAIVI
SRIGLIEIPYAMWEELIPALFKNIESGSEGIKQSTLQTLGYICEEIDPNVMSKYSDNVLR
VITDGIKDESQNVKLAGIQALCFALEFVKKNFDIK---

---LFNVFKNQSIIIYEEALLAIGAIILALDADFEPYFSNFLPILVNFLKNVDFGSVXNI
AIGIVGDLARAFGKKFSGVCAEIVPLIVSDLTNPKLSMNAKPSAISCLCDIAISVGADFI
PFLPMVMPILHQASTTEIDDEDFLNELRETIFQTYTGIIQGLKSDNQIEELNQYINPMLD
FVVLINDDPERYDSVTSAALALLGDLAQAMGDSIRNLLNNKIIREFVNNGIQKGIPYAHY
AILSIFPKKH*

Frame B:
tysskkwifiklwpmqiinlkvfvqkpnhf*pmhhnktfhylfth*h*nlqmkremykfv
n*qvlf*rivftlkiakemkf*lnnghqlnqmletslkilfykv*dhqnmkhvtl*ll*f
hvlvllkfhtqcgkn*sqhylktlkvvqkvlnnllfkhlvifvkkliqmlcqniqimy*e
llqmvskmnhkm*n*qvfkhyalh*nl*rkililk---

---ystysrinqssstkrlslplvpsswlwmlisnltfqisfqsl*tssrmsilvpxqtl
qlvslvi*lvllvrnslvfalksfh*slvispiqnsl*mlnqvlsvvsvisplvlvpisf
lsfqwlcqffikplplksmmktf*mnsvklsskltlvsfkvsrviiksknsintsiqc*t
llyl*mmiqnvmilllllpwpylvi*lklwvivleif*ttk*lenl*tmvfkkvyhmrim
xyyqffqknin

Frame C:
htpvkngslssfgqckssi*kcsfrsriifnqcittklsiiyslinirtyk*rerctnss
isrycfke*ysl*k*rkk*nfn*tmgin*tkc*krh*kysftrfritki*stshcsycnf
tywsy*nsirnvgrinpsii*kh*kwfrry*tiyssntwlyl*rn*skcyvkifr*ciks
yyrwyqr*itkckisryssimlcirickekf*y*r---

---iqriqesinhhlrrgspchwchhlgfgc*frtllfkfpsnpcklpqecrfwfrxkhc
nwyrw*fsscfw*eilwclr*nrsidr**shqsktlyec*tkcyqlsl*yrh*cwcrfhs
fpsngyansssslyh*nr**rlfk*tp*nylpnlhwyhsrsqe**snrrtqsihqsnvrl
cctyk**srtl*fcyfcclgltw*fsssyg**y*ksfkqqnn*rickqwyskryticalc
xiinfskktli

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS425 (CHS425Q) /CSM/CH/CHS4-B/CHS425Q.Seq.d/                       2680   0.0
VHD795 (VHD795Q) /CSM/VH/VHD7-D/VHD795Q.Seq.d/                       1421   0.0
CHR745 (CHR745Q) /CSM/CH/CHR7-B/CHR745Q.Seq.d/                       1415   0.0
VHO468 (VHO468Q) /CSM/VH/VHO4-C/VHO468Q.Seq.d/                       1413   0.0
VHH762 (VHH762Q) /CSM/VH/VHH7-C/VHH762Q.Seq.d/                       1413   0.0
CHQ722 (CHQ722Q) /CSM/CH/CHQ7-A/CHQ722Q.Seq.d/                       1413   0.0
AHM268 (AHM268Q) /CSM/AH/AHM2-C/AHM268Q.Seq.d/                       1413   0.0
VHP718 (VHP718Q) /CSM/VH/VHP7-A/VHP718Q.Seq.d/                       1407   0.0
VHP264 (VHP264Q) /CSM/VH/VHP2-C/VHP264Q.Seq.d/                       1407   0.0
VHP188 (VHP188Q) /CSM/VH/VHP1-D/VHP188Q.Seq.d/                       1407   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AP002799|AP002799.3 Homo sapiens genomic DNA, chromosome 11q, clone:RP11-806N19.    38   0.28   2
AC024715|AC024715.3 Homo sapiens chromosome 11 clone RP11-201M22, WORKING DRAFT SEQUENCE, 13 unordered pieces.    38   0.28   2
AL358112|AL358112.20 Human DNA sequence from clone RP11-311B1 on chromosome 6.    44   1.1    5
AC167958|AC167958.8 Medicago truncatula clone mth2-161m16, WORKING DRAFT SEQUENCE, 17 unordered pieces.    42   1.7    4
CV748470|CV748470.1 SJL_35_004__5-4__08.T7_012.ab1 SJL Schistosoma japonicum cDNA, mRNA sequence.    46   2.0    1
CV741544|CV741544.1 SJAL_033_76.T7 SJL Schistosoma japonicum cDNA, mRNA sequence.    46   2.0    1
CV740288|CV740288.1 SJAL_026_17.T7 SJL Schistosoma japonicum cDNA, mRNA sequence.    46   2.0    1
CV737780|CV737780.1 SJAL_010_44.T7 SJL Schistosoma japonicum cDNA, mRNA sequence.    46   2.0    1
BU802954|BU802954.1 SJFAPE03 SJF Schistosoma japonicum cDNA, mRNA sequence.    46   2.0    1
AC118385|AC118385.4 Rattus norvegicus clone CH230-42B13, WORKING DRAFT SEQUENCE, 1 ordered piece.    46   2.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE017344_109(AE017344|pid:none) Cryptococcus neoformans var. neo...   134   7e-30
BC052711_1(BC052711|pid:none) Mus musculus karyopherin (importin...   128   4e-28
(P70168) RecName: Full=Importin subunit beta-1; AltName: Full=Ka...   128   4e-28
AK314780_1(AK314780|pid:none) Homo sapiens cDNA, FLJ95650, highl...   127   9e-28
PDBN(1IBR)B MOL_ID: 1;MOL_ID: 1; MOLECULE: RAN; MOLECULE: RAN; C...   127   9e-28
(Q14974) RecName: Full=Importin subunit beta-1; AltName: Full=Ka...   127   9e-28
FM992688_205(FM992688|pid:none) Candida dubliniensis CD36 chromo...   120   1e-25
BX842622_14(BX842622|pid:none) Neurospora crassa DNA linkage gro...   119   3e-25
(P52296) RecName: Full=Importin subunit beta-1; AltName: Full=Ka...   118   6e-25
CR382135_96(CR382135|pid:none) Debaryomyces hansenii strain CBS7...   117   9e-25

 psg: 0.76  gvh: 0.42  alm: 0.26  top: 0.17  tms: 0.20  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: endoplasmic reticulum
	 16.0 %: plasma membrane
	 12.0 %: mitochondrial
	  8.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for CHS425 is end

>CHS425F.Seq
AACATACTCCAGTAAAAAATGGATCTTTATCAAGCTTTGGCCAATGCAAATCATCAATCT
GAAAGTGTTCGTTCAGAAGCCGAATCATTTTTAACCAATGCATCACAACAAAACTTTCCA
TTATTTATTCACTCATTAACATTAGAACTTACAAATGAAGAGAGAGATGTACAAATTCGT
CAATTAGCAGGTATTGTTTTAAAGAATAGTATTCACTCTAAAAATAGCGAAAGAAATGAA
ATTTTAATTAAACAATGGGCATCAATTGAACCAAATGCTAGAAACGTCATTAAAAATACT
CTTTTACAAGGTTTAGGATCACCAAAATATGAAGCACGTCACACTGTAGCTATTGTAATT
TCACGTATTGGTCTTATTGAAATTCCATACGCAATGTGGGAAGAATTAATCCCAGCATTA
TTTAAAAACATTGAAAGTGGTTCAGAAGGTATTAAACAATCTACTCTTCAAACACTTGGT
TATATTTGTGAAGAAATTGATCCAAATGTTATGTCAAAATATTCAGATAATGTATTAAGA
GTTATTACAGATGGTATCAAAGATGAATCACAAAATGTAAAATTAGCAGGTATTCAAGCA
TTATGCTTTGCATTAGAATTTGTAAAGAAAAATTTTGATATTAAAGANNNNNNNNNN

>CHS425Z.Seq
NNNNNNNNNNTTATTCAACGTATTCAAGAATCAATCAATCATCATCTACGAAGAGGCTCT
CCTTGCCATTGGTGCCATCATCTTGGCTTTGGATGCTGATTTCGAACCTTACTTTTCAAA
TTTCCTTCCAATCCTTGTAAACTTCCTCAAGAATGTCGATTTTGGTTCCGTANCAAACAT
TGCAATTGGTATCGTTGGTGATTTAGCTCGTGCTTTTGGTAAGAAATTCTCTGGTGTTTG
CGCTGAAATCGTTCCATTGATCGTTAGTGATCTCACCAATCCAAAACTCTCTATGAATGC
TAAACCAAGTGCTATCAGTTGTCTCTGTGATATCGCCATTAGTGTTGGTGCCGATTTCAT
TCCTTTCCTTCCAATGGTTATGCCAATTCTTCATCAAGCCTCTACCACTGAAATCGATGA
TGAAGACTTTTTAAATGAACTCCGTGAAACTATCTTCCAAACTTACACTGGTATCATTCA
AGGTCTCAAGAGTGATAATCAAATCGAAGAACTCAATCAATACATCAATCCAATGTTAGA
CTTTGTTGTACTTATAAATGATGATCCAGAACGTTATGATTCTGTTACTTCTGCTGCCTT
GGCCTTACTTGGTGATTTAGCTCAAGCTATGGGTGATAGTATTAGAAATCTTTTAAACAA
CAAAATAATTAGAGAATTTGTAAACAATGGTATTCAAAAAGGTATACCATATGCGCATTA
TGCNATATTATCAATTTTTCCAAAAAAACATTAATT

>CHS425P.Seq
AACATACTCCAGTAAAAAATGGATCTTTATCAAGCTTTGGCCAATGCAAATCATCAATCT
GAAAGTGTTCGTTCAGAAGCCGAATCATTTTTAACCAATGCATCACAACAAAACTTTCCA
TTATTTATTCACTCATTAACATTAGAACTTACAAATGAAGAGAGAGATGTACAAATTCGT
CAATTAGCAGGTATTGTTTTAAAGAATAGTATTCACTCTAAAAATAGCGAAAGAAATGAA
ATTTTAATTAAACAATGGGCATCAATTGAACCAAATGCTAGAAACGTCATTAAAAATACT
CTTTTACAAGGTTTAGGATCACCAAAATATGAAGCACGTCACACTGTAGCTATTGTAATT
TCACGTATTGGTCTTATTGAAATTCCATACGCAATGTGGGAAGAATTAATCCCAGCATTA
TTTAAAAACATTGAAAGTGGTTCAGAAGGTATTAAACAATCTACTCTTCAAACACTTGGT
TATATTTGTGAAGAAATTGATCCAAATGTTATGTCAAAATATTCAGATAATGTATTAAGA
GTTATTACAGATGGTATCAAAGATGAATCACAAAATGTAAAATTAGCAGGTATTCAAGCA
TTATGCTTTGCATTAGAATTTGTAAAGAAAAATTTTGATATTAAAGA----------TTA
TTCAACGTATTCAAGAATCAATCAATCATCATCTACGAAGAGGCTCTCCTTGCCATTGGT
GCCATCATCTTGGCTTTGGATGCTGATTTCGAACCTTACTTTTCAAATTTCCTTCCAATC
CTTGTAAACTTCCTCAAGAATGTCGATTTTGGTTCCGTANCAAACATTGCAATTGGTATC
GTTGGTGATTTAGCTCGTGCTTTTGGTAAGAAATTCTCTGGTGTTTGCGCTGAAATCGTT
CCATTGATCGTTAGTGATCTCACCAATCCAAAACTCTCTATGAATGCTAAACCAAGTGCT
ATCAGTTGTCTCTGTGATATCGCCATTAGTGTTGGTGCCGATTTCATTCCTTTCCTTCCA
ATGGTTATGCCAATTCTTCATCAAGCCTCTACCACTGAAATCGATGATGAAGACTTTTTA
AATGAACTCCGTGAAACTATCTTCCAAACTTACACTGGTATCATTCAAGGTCTCAAGAGT
GATAATCAAATCGAAGAACTCAATCAATACATCAATCCAATGTTAGACTTTGTTGTACTT
ATAAATGATGATCCAGAACGTTATGATTCTGTTACTTCTGCTGCCTTGGCCTTACTTGGT
GATTTAGCTCAAGCTATGGGTGATAGTATTAGAAATCTTTTAAACAACAAAATAATTAGA
GAATTTGTAAACAATGGTATTCAAAAAGGTATACCATATGCGCATTATGCNATATTATCA
ATTTTTCCAAAAAAACATTAATT