>CHS424 (CHS424Q) /CSM/CH/CHS4-A/CHS424Q.Seq.d/
XXXXXXXXXXTAATGATGGTAATGATTGGATGTGGAGTGAAAGATCACCATTAAATTCTG
GTCCAATGGTACAAATATTAGATTATCCAATTTTAATTGTTTATGGTACAATTGGAAGTG
AACAACAAACCAATCAACGTCAACAATCTGCAGTTTATTTATCAAATGTTTTAACGTATC
AAATGAGATATTCAATAAATGTTTTAGAGGATACTCAATTCACAAATGACTTATTCACAA
CTTATAATGTAATATTTATGGGAGGCCCACAAACAAATAAAGCAGTTTACTCAATTCAAT
CAGTTTTACCATCACCAATTGGTTTCCATGGTTCAAGTGATAATATGTCATTCTCTTTAA
ATGATGATTCAAATATGTTTTCAGGTCCTTCTGTTGGTTTATTGTTTTTATCATCATGTT
ATTCACCAGAGGCCATTATGAAAATTACACCAAAAGAACGTTATACTCAAGCTAGACAAG
GTTGTATGCTTACAGTTATTGAGGGTACTGATACTATTGGTTTCCAAAATAGTCTTTTCA
ATTTCCCAACTAAATCATCTTTCCCAATGCCAGATTTCATGATATTAGGTCCAAATTTTG
GTTATCAAGGTGTATCGGGT

---NDGNDWMWSERSPLNSGPMVQILDYPILIVYGTIGSEQQTNQRQQSAVYLSNVLTYQ
MRYSINVLEDTQFTNDLFTTYNVIFMGGPQTNKAVYSIQSVLPSPIGFHGSSDNMSFSLN
DDSNMFSGPSVGLLFLSSCYSPEAIMKITPKERYTQARQGCMLTVIEGTDTIGFQNSLFN
FPTKSSFPMPDFMILGPNFGYQGVSG

Frame A:
---NDGNDWMWSERSPLNSGPMVQILDYPILIVYGTIGSEQQTNQRQQSAVYLSNVLTYQ
MRYSINVLEDTQFTNDLFTTYNVIFMGGPQTNKAVYSIQSVLPSPIGFHGSSDNMSFSLN
DDSNMFSGPSVGLLFLSSCYSPEAIMKITPKERYTQARQGCMLTVIEGTDTIGFQNSLFN
FPTKSSFPMPDFMILGPNFGYQGVSG


Frame B:
---mmvmigcgvkdhh*ilvqwyky*iiqf*lfmvqlevnnkpinvnnlqfiyqmf*rik
*diq*mf*rilnsqmtysqlim*ylweahkqikqftqfnqfyhhqlvsmvqviichsl*m
miqicfqvlllvycfyhhvihqrpl*klhqknvilkldkvvclqllrvlillvskivfsi
sqlnhlsqcqis*y*vqilvikvyr


Frame C:
---*w**ldve*kitikfwsngtnirlsnfnclwynwk*ttnqststicslfikcfnvsn
eifnkcfrgysihk*lihnl*cniygrptnk*ssllnsisftitnwfpwfk**yvilfk*
*fkyvfrsfcwfivfiimlftrghyenytkrtlyss*trlyaysy*gy*yywfpk*sfqf
pn*iifpnarfhdirskfwlsrcig

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS424 (CHS424Q) /CSM/CH/CHS4-A/CHS424Q.Seq.d/                       1209   0.0
VHP513 (VHP513Q) /CSM/VH/VHP5-A/VHP513Q.Seq.d/                       1201   0.0
VHP233 (VHP233Q) /CSM/VH/VHP2-B/VHP233Q.Seq.d/                       1201   0.0
VHM570 (VHM570Q) /CSM/VH/VHM5-C/VHM570Q.Seq.d/                       1201   0.0
VHK792 (VHK792Q) /CSM/VH/VHK7-D/VHK792Q.Seq.d/                       1201   0.0
VHK206 (VHK206Q) /CSM/VH/VHK2-A/VHK206Q.Seq.d/                       1201   0.0
VHI417 (VHI417Q) /CSM/VH/VHI4-A/VHI417Q.Seq.d/                       1201   0.0
VHH656 (VHH656Q) /CSM/VH/VHH6-C/VHH656Q.Seq.d/                       1201   0.0
VHE491 (VHE491Q) /CSM/VH/VHE4-D/VHE491Q.Seq.d/                       1201   0.0
VHE145 (VHE145Q) /CSM/VH/VHE1-B/VHE145Q.Seq.d/                       1201   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC104154|AC104154.3 Pan troglodytes clone RP43-108N2, WORKING DRAFT SEQUENCE, 2 ordered pieces.    42   0.82   4
AP008069|AP008069.1 Lotus corniculatus var. japonicus genomic DNA, chromosome 3, clone:LjT42K10, TM1424.    46   0.87   1
AP007593|AP007593.1 Lotus corniculatus var. japonicus genomic DNA, chromosome 3, clone:LjT23O15, TM0792.    46   0.87   1
AG980980|AG980980.1 Drosophila simulans DNA, clone: DSM1-026L07.F.fa, genomic survey sequence.    42   1.2    2
BU809730|BU809730.1 UL61D03 Populus leaf cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    44   3.4    1
AV246107|AV246107.1 Mus musculus 0 day neonate head cDNA, RIKEN full-length enriched library, clone:4832405C10, 3' end partial sequence.    44   3.4    1
BY371675|BY371675.1 Mus musculus cDNA, RIKEN full-length enriched library, clone:G430091G23, 3' end partial sequence.    44   3.4    1
BV313117|BV313117.1 S236P666RC12.T0 AlaskanMalamute Canis familiaris STS genomic, sequence tagged site.    44   3.4    1
AC009684|AC009684.2 Homo sapiens clone RP11-85N11, LOW-PASS SEQUENCE SAMPLING.    44   3.4    1
AC124615|AC124615.11 Mus musculus chromosome 5, clone RP24-445F15, complete sequence.    44   3.4    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000267_695(CP000267|pid:none) Rhodoferax ferrireducens T118, c...    39   0.088
AC116957_137(AC116957|pid:none) Dictyostelium discoideum chromos...    38   0.26
AE017341_214(AE017341|pid:none) Cryptococcus neoformans var. neo...    37   0.33
FM992689_113(FM992689|pid:none) Candida dubliniensis CD36 chromo...    34   2.8
(Q5AD67) RecName: Full=ATP-dependent RNA helicase CHL1;         ...    34   3.7
(Q6BZD9) RecName: Full=ATP-dependent RNA helicase CHL1;         ...    33   6.3
CP000742_974(CP000742|pid:none) Methanococcus vannielii SB, comp...    33   8.2

 psg: 0.75  gvh: 0.46  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for CHS424 is cyt

>CHS424Z.Seq
NNNNNNNNNNTAATGATGGTAATGATTGGATGTGGAGTGAAAGATCACCATTAAATTCTG
GTCCAATGGTACAAATATTAGATTATCCAATTTTAATTGTTTATGGTACAATTGGAAGTG
AACAACAAACCAATCAACGTCAACAATCTGCAGTTTATTTATCAAATGTTTTAACGTATC
AAATGAGATATTCAATAAATGTTTTAGAGGATACTCAATTCACAAATGACTTATTCACAA
CTTATAATGTAATATTTATGGGAGGCCCACAAACAAATAAAGCAGTTTACTCAATTCAAT
CAGTTTTACCATCACCAATTGGTTTCCATGGTTCAAGTGATAATATGTCATTCTCTTTAA
ATGATGATTCAAATATGTTTTCAGGTCCTTCTGTTGGTTTATTGTTTTTATCATCATGTT
ATTCACCAGAGGCCATTATGAAAATTACACCAAAAGAACGTTATACTCAAGCTAGACAAG
GTTGTATGCTTACAGTTATTGAGGGTACTGATACTATTGGTTTCCAAAATAGTCTTTTCA
ATTTCCCAACTAAATCATCTTTCCCAATGCCAGATTTCATGATATTAGGTCCAAATTTTG
GTTATCAAGGTGTATCGGGT