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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS416
Library CH
(Link to library)
Clone ID CHS416
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16419-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>CHS416 (CHS416Q) /CSM/CH/CHS4-A/CHS416Q.Seq.d/
XXXXXXXXXXCCACCAAAACCACCAGTTTGTTTTTTAAGATGTCCACCAAATCATGAATG
TAGATTTAATGACCAAGGTCATCAATGCTGTGTAAAGGGTTCNCCATGATAGATGTTCAT
TAAGATGTCCACATGGCCATGAAATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTC
AGATCCCATAGACCACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAA
TGTAAATTTGATGATCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGT
GATTTAGATTGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGT
GTCCGTTCAGAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCA
GGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCA
CCACCAAAATGCTCTTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGT
GAAGAATGTTGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCAT
GAATGTAAAGTTGATCAACATGGTAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCA
AAATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAA
TGCTGTGTTAAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAA
GGTTTACATTGTGCCATGAAAACTGTACCAATTGATAAAGAAAATTGTTGGAAAAAGNNA
CAGAACNTTACTCCA
sequence update 2002. 9.10
Translated Amino Acid sequence 
---tkttslffkmstks*m*i**prssmlckgfxmidvh*DVHMAMKCKVDHNGKECCVR
SHRPPPPEVCSLRCPPKHECKFDDHGKKCCVKIHCDEVCDLDCGRGFECKIRHDGSKCCV
RSERPHPPQHEKCNKRCPPGHECKVDQHGKECCVVAHRPPPKCSLRCPPRHECRVNHFGE
ECCVKVHHDKCSLRCPPGHECKVDQHGKECCVVAHRPPPKCSLRCPPKHECRINHFGEEC
CVKSRNDCLTCEDLNCERKGLHCAMKTVPIDKENCWKKXQNXTP
Translated Amino Acid sequence (All Frames) 
Frame A:
---hqnhqfvf*dvhqimnvdlmtkvinav*rvxhdrcslrcphghem*s*sqw*rmlrq
ip*ttttrslfikmptkt*m*i**sw*kmlckdsl**sl*frlw*rf*m*n*t*wfkmlc
pfrkttpttt*km**emptrp*m*s*stwkrmlrccp*tttkmlfkmstkt*m*sqplw*
rmlc*sspr*mfiemstrp*m*s*stw*rmlrccp*tttkmlikmstkt*m*nqslw*rm
lc*k*k*lfnl*rpkl*kkrftlchenctn**rkllekxtexys


Frame B:
---tkttslffkmstks*m*i**prssmlckgfxmidvh*DVHMAMKCKVDHNGKECCVR
SHRPPPPEVCSLRCPPKHECKFDDHGKKCCVKIHCDEVCDLDCGRGFECKIRHDGSKCCV
RSERPHPPQHEKCNKRCPPGHECKVDQHGKECCVVAHRPPPKCSLRCPPRHECRVNHFGE
ECCVKVHHDKCSLRCPPGHECKVDQHGKECCVVAHRPPPKCSLRCPPKHECRINHFGEEC
CVKSRNDCLTCEDLNCERKGLHCAMKTVPIDKENCWKKXQNXTP


Frame C:
---pkppvcflrcppnhecrfndqghqccvkgsp**mfikmstwp*nvklitmvknvasd
pidhhhqkfvh*dahqnmnvnlmimvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvs
vqkdhthhnmknvirdahqamnvklinmeknvallpidhhqnal*dvhqdmnvesttlvk
nvvlkfttinvh*dvhqamnvklinmvknvallpidhhqnah*dvhqnmnvesitlvkna
vlkvemiv*lvkt*tvkekvyivp*klyqlikkivgkxxrtll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS416 (CHS416Q) /CSM/CH/CHS4-A/CHS416Q.Seq.d/                       1651   0.0
CHI869 (CHI869Q) /CSM/CH/CHI8-C/CHI869Q.Seq.d/                       1554   0.0
AHD377 (AHD377Q) /CSM/AH/AHD3-D/AHD377Q.Seq.d/                       1554   0.0
AHD145 (AHD145Q) /CSM/AH/AHD1-B/AHD145Q.Seq.d/                       1546   0.0
AHA296 (AHA296Q) /CSM/AH/AHA2-D/AHA296Q.Seq.d/                       1546   0.0
CHM150 (CHM150Q) /CSM/CH/CHM1-C/CHM150Q.Seq.d/                       1544   0.0
CHI528 (CHI528Q) /CSM/CH/CHI5-B/CHI528Q.Seq.d/                       1544   0.0
AHN244 (AHN244Q) /CSM/AH/AHN2-B/AHN244Q.Seq.d/                       1544   0.0
AHA358 (AHA358Q) /CSM/AH/AHA3-C/AHA358Q.Seq.d/                       1542   0.0
CHQ619 (CHQ619Q) /CSM/CH/CHQ6-A/CHQ619Q.Seq.d/                       1540   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.  1356   0.0    3
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.  1356   0.0    3
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.    74   2e-15  4
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.    74   5e-15  4
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    74   5e-09  1
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    56   0.001  1
Z97340|Z97340.2 Arabidopsis thaliana DNA chromosome 4, ESSA I FCA contig fragment  No. 5.    42   0.19   5
AC141955|AC141955.2 Rattus norvegicus clone CH230-418P10, WORKING DRAFT SEQUENCE, 29 unordered pieces.    48   0.31   1
AC127160|AC127160.3 Rattus norvegicus clone CH230-460D14, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.31   1
AC162511|AC162511.3 Bos taurus clone CH240-113F1, *** SEQUENCING IN PROGRESS ***, 45 unordered pieces.    46   0.36   4
dna update 2005.11.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   546   e-174
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   189   2e-48
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...   110   5e-24
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    79   1e-13
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        70   7e-11
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    70   9e-11
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    70   9e-11
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    67   9e-10
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    63   1e-08
FJ380206_1(FJ380206|pid:none) Haliotis discus discus antistasin-...    62   2e-08
protein update 2009. 5.21
PSORT 

 psg: 0.58  gvh: 0.23  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.07  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar

>> prediction for CHS416 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID CHS416Z
3' end seq. 
>CHS416Z.Seq
NNNNNNNNNNCCACCAAAACCACCAGTTTGTTTTTTAAGATGTCCACCAAATCATGAATG
TAGATTTAATGACCAAGGTCATCAATGCTGTGTAAAGGGTTCNCCATGATAGATGTTCAT
TAAGATGTCCACATGGCCATGAAATGTAAAGTTGATCACAATGGTAAAGAATGTTGCGTC
AGATCCCATAGACCACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAA
TGTAAATTTGATGATCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGT
GATTTAGATTGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGT
GTCCGTTCAGAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCA
GGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCA
CCACCAAAATGCTCTTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGT
GAAGAATGTTGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCAT
GAATGTAAAGTTGATCAACATGGTAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCA
AAATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAA
TGCTGTGTTAAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAA
GGTTTACATTGTGCCATGAAAACTGTACCAATTGATAAAGAAAATTGTTGGAAAAAGNNA
CAGAACNTTACTCCA
Length of 3' end seq. 855
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -