>CHS413 (CHS413Q) /CSM/CH/CHS4-A/CHS413Q.Seq.d/
ATTTTCGAAAACTTTAAAACACACTTTCATATAATTTTTTTTCTTTTATATTAAAATTTG
TTTAAATATAAAATGTTAAGATCATTAAAAGCTTTAAAACTTAACAGTTTAAATGCCCAA
AAAGGTATTCGTCAATTCTGTTCAGATTCTAAAATCTCTGGTCAAGTTATTGGTATTGAT
TTAGGTACAACCAATTCTTGTGTTGCTGTTATGCAAGGTGCTGAAGCCCGTGTTTTAGAA
AATGCTGAAGGTGGTCGTACCACTCCATCAGTTGTTGCCTTCACTGAAGACAACCAAAAA
ATCGTTGGTTTACCAGCTAAAAGACAAATGGTTACAAATGCTGAAAATACATTATTTGCT
ACTAAACGTTTAATTGGTCGTAGATTTGATGATCCAATGACTAAAAAAGATATGACAATG
GTACCATANTAAGATTGTTAAAGGTCCAAATGGTGATGCATNGTTTGAAGTTAAAGGTAA
AATGATTTCACCATCAGAAGCAGGTGCTATGGTTTTACAAAAGATGAAAGAAACCGCCGA
AACCAATTTAGGTGGCCCAGTTACAGATGCTGTTATTACCGTTCCAGCATACTTTGATGA
TAGTCAACGTCAAGCCACTCGTGATGCAGGTACAATTGCAXXXXXXXXXXGTCTTTACTC
GTTTAATCAAGAAAAATACTAATATTCCAGCCTCAAAGACTGATACTTTCTCAACTGCTG
CCGATGGTCAATCTGAAGTAGAAATTAAAGTTTTCCAAGGTGAAAGAGAAATGGCCGTCG
ACAATAAACTCCTCGCCAAATTCACTTTATTCGGTCTCCCACCATTACCAAAAGGTGTCC
CACAAATTGAAGTTACCTTTGATATTGATGTCAATGGTATGTTACAAGTTATTGCTAAAG
ATAAAGCTACCGGTAAAGCTCAACAAGTTAGAATTCAAACCAGTGGTGGTTTAAGCAAAG
ATGATATCGCTCGTATCCTTAAAGAATCTGAAGCCAACGCTGAAGTTGATCGTAAACGTA
AAGAATTAGTTGAAGCCAGAAACAATGGTGAATCCGTTGTATACCAAGTTGAAAAAGATT
TAGTTGAATTCAAAGATTACCTCTCTCAACCACAAACTGAAGAAATTAAAAAAGCAGTCC
AAGCCGTTCGTGATGTCCTTGCCAAAGAAGATGGTTCAGCCATTGAAGCTGAAATTAAAA
AATTACACGATTTAACTCGTTCATCTTTTGAAACTGCCTACAAAGCTAAACTCGATTCAT
CTGCAAGCAAATCTTCATCAACTGAAAATAAAGAAAACAAAGATAACACCACGAAGCTGA
ATTCAC

fsktlkhtfi*ffffyikiclnikc*dh*kl*nltv*mpkkvfvnsvqilkslvkllvli
*vqpilvlllckvlkpvf*kmlkvvvplhqllpslkttkkslvyqlkdkwlqmlkihyll
lnv*lvvdlmiq*lkki*QWYHXKIVKGPNGDAXFEVKGKMISPSEAGAMVLQKMKETAE
TNLGGPVTDAVITVPAYFDDSQRQATRDAGTIA---

---VFTRLIKKNTNIPASKTDTFSTAADGQSEVEIKVFQGEREMAVDNKLLAKFTLFGLP
PLPKGVPQIEVTFDIDVNGMLQVIAKDKATGKAQQVRIQTSGGLSKDDIARILKESEANA
EVDRKRKELVEARNNGESVVYQVEKDLVEFKDYLSQPQTEEIKKAVQAVRDVLAKEDGSA
IEAEIKKLHDLTRSSFETAYKAKLDSSASKSSSTENKENKDNTTKLNS

Frame A:
ifenfkthfhiifflly*nlfkykmlrslkalklnslnaqkgirqfcsdskisgqvigid
lgttnscvavmqgaearvlenaeggrttpsvvaftednqkivglpakrqmvtnaentlfa
tkrligrrfddpmtkkdmtmvpx*dc*rskw*cxv*s*r*ndftirsrcygftkdernrr
nqfrwpsyrccyyrssil***stsshs*crync---

---VFTRLIKKNTNIPASKTDTFSTAADGQSEVEIKVFQGEREMAVDNKLLAKFTLFGLP
PLPKGVPQIEVTFDIDVNGMLQVIAKDKATGKAQQVRIQTSGGLSKDDIARILKESEANA
EVDRKRKELVEARNNGESVVYQVEKDLVEFKDYLSQPQTEEIKKAVQAVRDVLAKEDGSA
IEAEIKKLHDLTRSSFETAYKAKLDSSASKSSSTENKENKDNTTKLNS

Frame B:
fsktlkhtfi*ffffyikiclnikc*dh*kl*nltv*mpkkvfvnsvqilkslvkllvli
*vqpilvlllckvlkpvf*kmlkvvvplhqllpslkttkkslvyqlkdkwlqmlkihyll
lnv*lvvdlmiq*lkki*QWYHXKIVKGPNGDAXFEVKGKMISPSEAGAMVLQKMKETAE
TNLGGPVTDAVITVPAYFDDSQRQATRDAGTIA---

---sllv*srkilifqpqrlilsqllpmvnlk*klkfskvkekwpstinsspnslysvsh
hyqkvshklklplilmsmvcyklllkiklpvklnklefkpvvv*akmislvslknlkptl
klivnvkn*lkpetmvnplytklkki*lnskitslnhklkklkkqskpfvmslpkkmvqp
lklklknyti*lvhllklptklnsihlqanlhqlkikktkitprs*ih

Frame C:
frkl*ntlsynffsfilkfv*i*nvkiiksfkt*qfkcpkryssilfrf*nlwssywy*f
rynqflcccyarc*spcfrkc*rwsyhsiscclh*rqpknrwfts*ktngykc*kyiicy
*tfnws*i**snd*krydngtixrllkvqmvmhxlklkvk*fhhqkqvlwfykr*kkppk
pi*vaqlqmlllpfqhtlmivnvkplvmqvql---

---lysfnqeky*ysslkd*yflnccrwsi*srn*sfpr*krngrrq*tprqihfirspt
itkrcptn*syl*y*cqwyvtsyc*r*syr*ssts*nsnqwwfkqr*yrsyp*ri*sqr*
s*s*t*ris*sqkqw*ircips*krfs*iqrlplsttn*rn*ksspsrs*cpcqrrwfsh
*s*n*kitrfnsfif*nclqs*trfickqifin*k*rkqr*hheaef

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS413 (CHS413Q) /CSM/CH/CHS4-A/CHS413Q.Seq.d/                       2510   0.0
VHC706 (VHC706Q) /CSM/VH/VHC7-A/VHC706Q.Seq.d/                       1340   0.0
CHM184 (CHM184Q) /CSM/CH/CHM1-D/CHM184Q.Seq.d/                       1326   0.0
CHL664 (CHL664Q) /CSM/CH/CHL6-C/CHL664Q.Seq.d/                       1326   0.0
CHI781 (CHI781Q) /CSM/CH/CHI7-D/CHI781Q.Seq.d/                       1326   0.0
CHI137 (CHI137Q) /CSM/CH/CHI1-B/CHI137Q.Seq.d/                       1326   0.0
CFI557 (CFI557Q) /CSM/CF/CFI5-C/CFI557Q.Seq.d/                       1326   0.0
VHB739 (VHB739Q) /CSM/VH/VHB7-B/VHB739Q.Seq.d/                       1324   0.0
VHC763 (VHC763Q) /CSM/VH/VHC7-C/VHC763Q.Seq.d/                       1320   0.0
VHQ334 (VHQ334Q) /CSM/VH/VHQ3-B/VHQ334Q.Seq.d/                       1318   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF539617|AF539617.1 Dictyostelium discoideum mitochondrial Hsp70 precursor (mhsp70) mRNA, complete cds; nuclear gene for mitochondrial product.  1312   0.0    2
AY170440|AY170440.1 Dictyostelium discoideum mitochondrial heat shock protein Hsp70 (hsp70) gene, complete cds; nuclear gene for mitochondrial product.  1312   0.0    2
AF419853|AF419853.1 Neocallimastix patriciarum heat shock protein 70 precursor (Hsp70) mRNA, complete cds.    76   1e-32  5
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.           68   6e-28  6
A58313|A58313.1 Sequence 1 from Patent WO9636707.                  68   3e-24  5
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.    68   5e-24  5
AB050740|AB050740.1 Plasmodium falciparum mRNA for heat shock protein hsp70 homologue Pfhsp70-3, complete cds.    68   1e-23  6
AR377080|AR377080.1 Sequence 2086 from patent US 6605709.          56   2e-23  6
D88673|D88673.1 Buchnera sp. DNA for DnaK, DnaJ.                   48   2e-23  8
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.    54   6e-22  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8I0H7) RecName: Full=Heat shock 70 kDa protein, mitochondrial;...   382   e-104
EU306563_1(EU306563|pid:none) Tigriopus japonicus heat shock pro...   217   8e-55
DQ524993_1(DQ524993|pid:none) Sparus aurata glucose regulated pr...   214   1e-54
AD3502(AD3502) dnaK protein [imported] - Brucella melitensis (st...   216   1e-54
(Q8YE76) RecName: Full=Chaperone protein dnaK; AltName: Full=Hea...   216   1e-54
BC067910_1(BC067910|pid:none) Xenopus tropicalis heat shock 70kD...   216   1e-54
BC044175_1(BC044175|pid:none) Danio rerio heat shock protein 9, ...   214   2e-54
BC083504_1(BC083504|pid:none) Danio rerio heat shock protein 9, ...   210   2e-54
AB168862_1(AB168862|pid:none) Macaca fascicularis testis cDNA, c...   214   7e-54
(Q3ZCH0) RecName: Full=Stress-70 protein, mitochondrial; AltName...   214   7e-54

 psg: 0.68  gvh: 0.29  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for CHS413 is cyt

>CHS413F.Seq
ATTTTCGAAAACTTTAAAACACACTTTCATATAATTTTTTTTCTTTTATATTAAAATTTG
TTTAAATATAAAATGTTAAGATCATTAAAAGCTTTAAAACTTAACAGTTTAAATGCCCAA
AAAGGTATTCGTCAATTCTGTTCAGATTCTAAAATCTCTGGTCAAGTTATTGGTATTGAT
TTAGGTACAACCAATTCTTGTGTTGCTGTTATGCAAGGTGCTGAAGCCCGTGTTTTAGAA
AATGCTGAAGGTGGTCGTACCACTCCATCAGTTGTTGCCTTCACTGAAGACAACCAAAAA
ATCGTTGGTTTACCAGCTAAAAGACAAATGGTTACAAATGCTGAAAATACATTATTTGCT
ACTAAACGTTTAATTGGTCGTAGATTTGATGATCCAATGACTAAAAAAGATATGACAATG
GTACCATANTAAGATTGTTAAAGGTCCAAATGGTGATGCATNGTTTGAAGTTAAAGGTAA
AATGATTTCACCATCAGAAGCAGGTGCTATGGTTTTACAAAAGATGAAAGAAACCGCCGA
AACCAATTTAGGTGGCCCAGTTACAGATGCTGTTATTACCGTTCCAGCATACTTTGATGA
TAGTCAACGTCAAGCCACTCGTGATGCAGGTACAATTGCANNNNNNNNNN

>CHS413Z.Seq
NNNNNNNNNNGTCTTTACTCGTTTAATCAAGAAAAATACTAATATTCCAGCCTCAAAGAC
TGATACTTTCTCAACTGCTGCCGATGGTCAATCTGAAGTAGAAATTAAAGTTTTCCAAGG
TGAAAGAGAAATGGCCGTCGACAATAAACTCCTCGCCAAATTCACTTTATTCGGTCTCCC
ACCATTACCAAAAGGTGTCCCACAAATTGAAGTTACCTTTGATATTGATGTCAATGGTAT
GTTACAAGTTATTGCTAAAGATAAAGCTACCGGTAAAGCTCAACAAGTTAGAATTCAAAC
CAGTGGTGGTTTAAGCAAAGATGATATCGCTCGTATCCTTAAAGAATCTGAAGCCAACGC
TGAAGTTGATCGTAAACGTAAAGAATTAGTTGAAGCCAGAAACAATGGTGAATCCGTTGT
ATACCAAGTTGAAAAAGATTTAGTTGAATTCAAAGATTACCTCTCTCAACCACAAACTGA
AGAAATTAAAAAAGCAGTCCAAGCCGTTCGTGATGTCCTTGCCAAAGAAGATGGTTCAGC
CATTGAAGCTGAAATTAAAAAATTACACGATTTAACTCGTTCATCTTTTGAAACTGCCTA
CAAAGCTAAACTCGATTCATCTGCAAGCAAATCTTCATCAACTGAAAATAAAGAAAACAA
AGATAACACCACGAAGCTGAATTCAC

>CHS413P.Seq
ATTTTCGAAAACTTTAAAACACACTTTCATATAATTTTTTTTCTTTTATATTAAAATTTG
TTTAAATATAAAATGTTAAGATCATTAAAAGCTTTAAAACTTAACAGTTTAAATGCCCAA
AAAGGTATTCGTCAATTCTGTTCAGATTCTAAAATCTCTGGTCAAGTTATTGGTATTGAT
TTAGGTACAACCAATTCTTGTGTTGCTGTTATGCAAGGTGCTGAAGCCCGTGTTTTAGAA
AATGCTGAAGGTGGTCGTACCACTCCATCAGTTGTTGCCTTCACTGAAGACAACCAAAAA
ATCGTTGGTTTACCAGCTAAAAGACAAATGGTTACAAATGCTGAAAATACATTATTTGCT
ACTAAACGTTTAATTGGTCGTAGATTTGATGATCCAATGACTAAAAAAGATATGACAATG
GTACCATANTAAGATTGTTAAAGGTCCAAATGGTGATGCATNGTTTGAAGTTAAAGGTAA
AATGATTTCACCATCAGAAGCAGGTGCTATGGTTTTACAAAAGATGAAAGAAACCGCCGA
AACCAATTTAGGTGGCCCAGTTACAGATGCTGTTATTACCGTTCCAGCATACTTTGATGA
TAGTCAACGTCAAGCCACTCGTGATGCAGGTACAATTGCA----------GTCTTTACTC
GTTTAATCAAGAAAAATACTAATATTCCAGCCTCAAAGACTGATACTTTCTCAACTGCTG
CCGATGGTCAATCTGAAGTAGAAATTAAAGTTTTCCAAGGTGAAAGAGAAATGGCCGTCG
ACAATAAACTCCTCGCCAAATTCACTTTATTCGGTCTCCCACCATTACCAAAAGGTGTCC
CACAAATTGAAGTTACCTTTGATATTGATGTCAATGGTATGTTACAAGTTATTGCTAAAG
ATAAAGCTACCGGTAAAGCTCAACAAGTTAGAATTCAAACCAGTGGTGGTTTAAGCAAAG
ATGATATCGCTCGTATCCTTAAAGAATCTGAAGCCAACGCTGAAGTTGATCGTAAACGTA
AAGAATTAGTTGAAGCCAGAAACAATGGTGAATCCGTTGTATACCAAGTTGAAAAAGATT
TAGTTGAATTCAAAGATTACCTCTCTCAACCACAAACTGAAGAAATTAAAAAAGCAGTCC
AAGCCGTTCGTGATGTCCTTGCCAAAGAAGATGGTTCAGCCATTGAAGCTGAAATTAAAA
AATTACACGATTTAACTCGTTCATCTTTTGAAACTGCCTACAAAGCTAAACTCGATTCAT
CTGCAAGCAAATCTTCATCAACTGAAAATAAAGAAAACAAAGATAACACCACGAAGCTGA
ATTCAC