>CHS391 (CHS391Q) /CSM/CH/CHS3-D/CHS391Q.Seq.d/
ATATACATATATATATATAAAGAATATTTAATTAAATTCAATTTTATATATATATATAAA
AAGATGAAAACTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATA
AGTTCAACATATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTC
ATTGAAAAACCACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATT
TATCCAAAGAAACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTT
GTTGATAATTTAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTG
GATATTACAACATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATAT
TATGCTAAAACTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCA
GGTGATATTCATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACC
ATTGTTATCGAAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACAT
GGTGCAAATTATGTTCACAAAGCAACXXXXXXXXXXANCCAATCAANCCTCAATCGATGA
AGCCGTTGAATTGGCTCAATCATCAGATGTTGTTGTCGTTGTAATTGGTGAACTTCCAGA
AGCTGAAACTCCAGGTGATATTTATGATCTCTCTATGGATCCAAATGAAGTACTCTTATT
ACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATTTTGGTTGAAGCTCGTCCAAG
AATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTTTTAATGGCTTATCTCCCAGG
TAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGTAATGTAAATCCATCTGGTCG
TCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATA
CAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGTGATGGTCTTTCTTATACCAC
TTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCAATCTCTGGTCAATCTGGTAA
CTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAA
CGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCC
AGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTTATGCCAGCTAAATCTCAACA
AATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGGTGTCGATAATAAAATTAC
TTTAGAATCGGTCAATTCATCATTA

IYIYIYKEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEV
IEKPRTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQL
DITTLTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQT
IVIEGSPNKIPMIYGLDSVHGANYVHKA---

---XNQXSIDEAVELAQSSDVVVVVIGELPEAETPGDIYDLSMDPNEVLLLQQLVDTGKP
VVLILVEARPRILPPDLVYSCAAVLMAYLPGSEGGKPIANILMGNVNPSGRLPLTYPGTT
GDIGVPYYHKYSENGVTTPLFQFGDGLSYTTFNYTNLACSNCKPISGQSGNYTGVLGQSY
TFTVTVTNNGNVQGKDSVLLYLSDLWAQVTPEVKMLRGFQKVDLMPAKSQQISFTLNAYE
FSFIGVDNKITLESVNSSL

Frame A:
IYIYIYKEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEV
IEKPRTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQL
DITTLTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQT
IVIEGSPNKIPMIYGLDSVHGANYVHKA---

---xqsxlnr*sr*igsiircccrcnw*tsrs*nsr*yl*slygsk*stlittig*hw*t
ssfnfg*sssknfttrsrlqlccsfnglspr**rw*tnrqhshg*cksiwsssfnlsryy
w*yrcsilpqiq*kwchhstlpiw*wsflyhf*lyqsrl*ql*tnlwsiw*lhwcfrsil
yfhchchkqw*rsr*rlcslisl*slgssysrs*nvkrfpks*syas*istnlfypqcl*
illywcr**nyfrigqfii

Frame B:
ytyiyikni*lnsilyiyikr*kllkvyfychc***iy**vqhmevqle*allevkklks
lknhvlleikensn*sivkfiqrnn*ikkilisclpeilllii*cqkcqslkklvk*lnw
ilqh*hhqiqlqlmkqh*himlkliildhi*ihqyqvv*qvifiilivvfg*i*lilfkp
llskvvqirfq*fmv*iqymvqimftkq---

---XNQXSIDEAVELAQSSDVVVVVIGELPEAETPGDIYDLSMDPNEVLLLQQLVDTGKP
VVLILVEARPRILPPDLVYSCAAVLMAYLPGSEGGKPIANILMGNVNPSGRLPLTYPGTT
GDIGVPYYHKYSENGVTTPLFQFGDGLSYTTFNYTNLACSNCKPISGQSGNYTGVLGQSY
TFTVTVTNNGNVQGKDSVLLYLSDLWAQVTPEVKMLRGFQKVDLMPAKSQQISFTLNAYE
FSFIGVDNKITLESVNSSL

Frame C:
ihiyi*rifn*iqfyiyi*kdeny*kfiftvivdsefinkfniwkfn*skhcwr*rs*sh
*kttyfwk*krtqirv*snlsketiksrky*fhvcqryfc**fnvknvnh*knwsndsig
yyninitkynyn**nnisilc*nllywiifkftsirwfsr*yssy***clvryd*yysnh
cyrr*sk*dsndlwfrfstwcklcsqsn---

---pixpqsmkplnwlnhqmllsl*lvnfqklklqvifmislwiqmkysyynnwltlvnq
*f*fwlklvqefyhqisstvvlqf*wlisqvvkvvnqsptfswvm*ihlvvfl*liqvll
visvfhittntvkmvsplhssnlvmvfliplltipispvaivnqslvnlvttlvf*vnpi
lslslsqtmvtfkvktlfsyislifglkllqklkc*evskklilcqlnlnksllpsmpmn
spllvsiikll*nrsihh

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS391 (CHS391Q) /CSM/CH/CHS3-D/CHS391Q.Seq.d/                       2599   0.0
VHA583 (VHA583Q) /CSM/VH/VHA5-D/VHA583Q.Seq.d/                       1515   0.0
CHL451 (CHL451Q) /CSM/CH/CHL4-C/CHL451Q.Seq.d/                       1515   0.0
CHS263 (CHS263Q) /CSM/CH/CHS2-C/CHS263Q.Seq.d/                       1507   0.0
VHB608 (VHB608Q) /CSM/VH/VHB6-A/VHB608Q.Seq.d/                       1501   0.0
VHA533 (VHA533Q) /CSM/VH/VHA5-B/VHA533Q.Seq.d/                       1497   0.0
CHF295 (CHF295Q) /CSM/CH/CHF2-D/CHF295Q.Seq.d/                       1497   0.0
VHJ810 (VHJ810Q) /CSM/VH/VHJ8-A/VHJ810Q.Seq.d/                       1495   0.0
VHI472 (VHI472Q) /CSM/VH/VHI4-C/VHI472Q.Seq.d/                       1495   0.0
VHD112 (VHD112Q) /CSM/VH/VHD1-A/VHD112Q.Seq.d/                       1491   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L21014|L21014.1 Dictyostelium discoideum beta-glucosidase mRNA, complete cds.  1126   0.0    1
BG139849|BG139849.1 EST480291 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP14B10 5' sequence, mRNA sequence.    48   0.11   2
CN215175|CN215175.1 28949 Suspension culture Solanum tuberosum cDNA, mRNA sequence.    40   0.27   3
AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    38   0.49   8
BQ969550|BQ969550.1 QHB38N02.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB38N02, mRNA sequence.    48   0.51   1
AC073286|AC073286.5 Homo sapiens chromosome 3 clone RP11-298M2, *** SEQUENCING IN PROGRESS ***, 47 unordered pieces.    48   0.51   1
AC067915|AC067915.2 Homo sapiens clone RP11-131G15, WORKING DRAFT SEQUENCE, 21 unordered pieces.    48   0.51   1
AC022656|AC022656.3 Homo sapiens clone RP11-28P11, WORKING DRAFT SEQUENCE, 22 unordered pieces.    48   0.51   1
AL121949|AL121949.13 Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP3-407E4.    48   0.51   1
BF187215|BF187215.1 EST443502 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA39D5 5' sequence, mRNA sequence.    46   2.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   472   e-131
CP000159_2777(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   177   8e-43
CP000383_3519(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   160   1e-37
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...   157   8e-37
CP000383_2243(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   149   2e-34
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   145   2e-33
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...   135   3e-30
CP000685_3497(CP000685|pid:none) Flavobacterium johnsoniae UW101...   135   4e-30
CP000964_1565(CP000964|pid:none) Klebsiella pneumoniae 342, comp...   131   6e-29
CP001251_792(CP001251|pid:none) Dictyoglomus turgidum DSM 6724, ...   130   1e-28

 psg: 0.78  gvh: 0.73  alm: 0.26  top: 0.07  tms: 0.07  mit: 0.30  mip: 0.09
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 1.25  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for CHS391 is cyt

>CHS391F.Seq
ATATACATATATATATATAAAGAATATTTAATTAAATTCAATTTTATATATATATATAAA
AAGATGAAAACTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATA
AGTTCAACATATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTC
ATTGAAAAACCACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATT
TATCCAAAGAAACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTT
GTTGATAATTTAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTG
GATATTACAACATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATAT
TATGCTAAAACTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCA
GGTGATATTCATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACC
ATTGTTATCGAAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACAT
GGTGCAAATTATGTTCACAAAGCAACNNNNNNNNNN

>CHS391Z.Seq
NNNNNNNNNNANCCAATCAANCCTCAATCGATGAAGCCGTTGAATTGGCTCAATCATCAG
ATGTTGTTGTCGTTGTAATTGGTGAACTTCCAGAAGCTGAAACTCCAGGTGATATTTATG
ATCTCTCTATGGATCCAAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAAC
CAGTAGTTTTAATTTTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACA
GTTGTGCTGCAGTTTTAATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCA
ACATTCTCATGGGTAATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTA
CTGGTGATATCGGTGTTCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCAC
TCTTCCAATTTGGTGATGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTA
GCAATTGTAAACCAATCTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCT
ATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCT
TATATCTCTCTGATCTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCC
AAAAAGTTGATCTTATGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATG
AATTCTCCTTTATTGGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTA

>CHS391P.Seq
ATATACATATATATATATAAAGAATATTTAATTAAATTCAATTTTATATATATATATAAA
AAGATGAAAACTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATA
AGTTCAACATATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTC
ATTGAAAAACCACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATT
TATCCAAAGAAACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTT
GTTGATAATTTAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTG
GATATTACAACATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATAT
TATGCTAAAACTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCA
GGTGATATTCATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACC
ATTGTTATCGAAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACAT
GGTGCAAATTATGTTCACAAAGCAAC----------ANCCAATCAANCCTCAATCGATGA
AGCCGTTGAATTGGCTCAATCATCAGATGTTGTTGTCGTTGTAATTGGTGAACTTCCAGA
AGCTGAAACTCCAGGTGATATTTATGATCTCTCTATGGATCCAAATGAAGTACTCTTATT
ACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATTTTGGTTGAAGCTCGTCCAAG
AATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTTTTAATGGCTTATCTCCCAGG
TAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGTAATGTAAATCCATCTGGTCG
TCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATA
CAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGTGATGGTCTTTCTTATACCAC
TTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCAATCTCTGGTCAATCTGGTAA
CTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAA
CGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCC
AGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTTATGCCAGCTAAATCTCAACA
AATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGGTGTCGATAATAAAATTAC
TTTAGAATCGGTCAATTCATCATTA