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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS348
Library CH
(Link to library)
Clone ID CHS348
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16361-1
Original site URL
Representative seq. ID CHS348P
(Link to Original site)
Representative DNA sequence 
>CHS348 (CHS348Q) /CSM/CH/CHS3-B/CHS348Q.Seq.d/
AGATAAAGAGTTGTCATCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAA
ACATGTCGCCAAAGAAGATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGA
GGTTGTATTTGAAGTTAAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGT
ATTTTTAATTCGGAATATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAAC
CGATTATATCACATTCTCTTATTCAATCCTTTTCATCATTGTAGAGTTTATCTCATTTTT
GGGTTCAGCATTGCATTTGAATAACTTTACCAATCCATGTACTTTTGTTTTGGTGGTAAC
ATTGGAACAAATCCTTGCCAAGAGAAGAAAGAAGCATCCXXXXXXXXXXTCCATTAATTA
TGAGTATTTGGACTGTACCAATCGTTGTAAAAGATCCAATCATTTTCATTCTCGTCGGTA
TGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATATATGATTCTTAGAGCCAAGAGAC
CTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGTAGAAGCTACTGATCTTTGGAGAG
CTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATATTTCATCATGGAGAGAAGGTTCCG
CTTCAATCGTTAAACTTCTTAAAGCTAGAAAGATTAGTCGTCATAAGTTAGCCATGTGGA
ATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTGTGAAGTTTTCCGTCAAACTAAAT
TGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGGTAAACATAAGGCTGAACAATCTT
TCCGTACTTCAAACAAAGAATCTGATACCATTAAAAATTCAAGACTTTTCCTTCCAAATA
TTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAGTGCTGTACTTAGATTTAATTGTT
TCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGGTTTCTCTTTCTCTACCTTATGGC
ATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAGTNAAAAACAATGGCCGTATGCTT
CTTCATACCATGCTCACAATAT
sequence update 2002.10.25
Translated Amino Acid sequence 
DKELSSQKKKPSSMQLSKKKHVAKEDSETLETIIGEEKKEVVFEVKPYFSHAILQATMAV
FLIRNIFYFAYRAGWTMNRTDYITFSYSILFIIVEFISFLGSALHLNNFTNPCTFVLVVT
LEQILAKRRKKH---

---PLIMSIWTVPIVVKDPIIFILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLWVE
ATDLWRAEQTFFGFAGTYISSWREGSASIVKLLKARKISRHKLAMWNWKRDFVKKPVVCE
VFRQTKLVNENDNAQESSGKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMMSA
VLRFNCFQNDMWLLVVVAGFSFSTLWHLWSFIPMALRQSXKQWPYASSYHAHN
Translated Amino Acid sequence (All Frames) 
Frame A:
r*rvvitkeetiinaik*ketcrqrrf*nirdnhw*reeggci*s*tilfscnsssnhgg
ifnseyfllciscrldne*nrlyhillfnpfhhcrvylifgfsiafe*lyqsmyfcfggn
igtnpcqekkeas---

---sinyeyldctnrckrsnhfhsrrydscngvtkshsiyds*sqetl*sr*ewsitlgr
sy*sles*tnflwfcwylyfimerrfrfnr*ts*s*kd*ss*vshveleerfrketssl*
sfpsn*igq*k**csrikw*t*g*tifpyfkqri*yh*kfktfpskyyplcc*ylgydec
ct*i*lfpk*yvafgcrswflflylmafmvfhsngssskxktmavcffipcsqy

Frame B:
DKELSSQKKKPSSMQLSKKKHVAKEDSETLETIIGEEKKEVVFEVKPYFSHAILQATMAV
FLIRNIFYFAYRAGWTMNRTDYITFSYSILFIIVEFISFLGSALHLNNFTNPCTFVLVVT
LEQILAKRRKKH---

---PLIMSIWTVPIVVKDPIIFILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLWVE
ATDLWRAEQTFFGFAGTYISSWREGSASIVKLLKARKISRHKLAMWNWKRDFVKKPVVCE
VFRQTKLVNENDNAQESSGKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMMSA
VLRFNCFQNDMWLLVVVAGFSFSTLWHLWSFIPMALRQSXKQWPYASSYHAHN

Frame C:
ikschhkrrnhhqcn*vkrnmspkkilkh*rqslvkrrrrlylklnhtflmqffkqpwry
f*fgifftlhivpvgq*iepiishsliqsfssl*slshfwvqhci*itlpihvllfww*h
wnkslpreersi---

---h*l*vfglyqsl*kiqsfsfssv*fl*wcyqesfni*fleprdlmkpvrvvhhfg*k
llifgelnklslvllvlifhhgekvplqslnflklerlvvis*pcgigreis*rnq*fvk
fsvklnwsmkmimlknqvvnirlnnlsvlqtknliplkiqdfsfqilssllliswl**vl
yldlivskmicgfwls*lvslslpygiyglsfqwlfvkvknngrmllhtmlti
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS348 (CHS348Q) /CSM/CH/CHS3-B/CHS348Q.Seq.d/                       2119   0.0
AHO874 (AHO874Q) /CSM/AH/AHO8-D/AHO874Q.Seq.d/                       1360   0.0
SHK508 (SHK508Q) /CSM/SH/SHK5-A/SHK508Q.Seq.d/                       1360   0.0
SHK418 (SHK418Q) /CSM/SH/SHK4-A/SHK418Q.Seq.d/                       1360   0.0
SHG755 (SHG755Q) /CSM/SH/SHG7-C/SHG755Q.Seq.d/                       1360   0.0
SHG576 (SHG576Q) /CSM/SH/SHG5-D/SHG576Q.Seq.d/                       1360   0.0
SHG522 (SHG522Q) /CSM/SH/SHG5-A/SHG522Q.Seq.d/                       1360   0.0
SHG486 (SHG486Q) /CSM/SH/SHG4-D/SHG486Q.Seq.d/                       1360   0.0
SHG409 (SHG409Q) /CSM/SH/SHG4-A/SHG409Q.Seq.d/                       1360   0.0
SHG402 (SHG402Q) /CSM/SH/SHG4-A/SHG402Q.Seq.d/                       1360   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF163835|AF163835.1 Dictyostelium discoideum cellulose synthase (dcsA) mRNA, complete cds.  1360   0.0    1
AL354663|AL354663.6 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP11-242L16.    40   3.7    3
CX655723|CX655723.1 PO02039A06 Poplar SC cDNA library Populus alba x Populus tremula var. glandulosa cDNA clone PO02039A06 5', mRNA sequence.    44   6.2    1
CX175850|CX175850.1 B04_69-65_04.ab1 leaf inoculated with Marssonia pathogen of Populus deltoides Populus deltoides cDNA, mRNA sequence.    44   6.2    1
CA929806|CA929806.1 MTU2CA.P3.A01 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    44   6.2    1
CA929196|CA929196.1 MTU2CA.P10.C05 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    44   6.2    1
AC152510|AC152510.3 Bos taurus clone CH240-16D7, WORKING DRAFT SEQUENCE, 24 unordered pieces.    44   6.2    1
AC125647|AC125647.5 Rattus norvegicus clone CH230-231J3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    44   6.2    1
AC119192|AC119192.3 Mus musculus clone RP24-178H23, WORKING DRAFT SEQUENCE, 6 unordered pieces.    44   6.2    1
AC098306|AC098306.4 Rattus norvegicus clone CH230-1N5, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    44   6.2    1
dna update 2005.11.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9U720) RecName: Full=Cellulose synthase catalytic subunit A [U...   468   e-130
CP001279_992(CP001279|pid:none) Nautilia profundicola AmH, compl...    40   0.14
CP001048_907(CP001048|pid:none) Yersinia pseudotuberculosis PB1/...    35   3.4
CP001025_1222(CP001025|pid:none) Burkholderia ambifaria MC40-6 c...    35   3.4
CP000431_1654(CP000431|pid:none) Rhodococcus jostii RHA1, comple...    35   3.4
AE014186_198(AE014186|pid:none) Plasmodium falciparum 3D7 chromo...    35   3.4
CP000102_1408(CP000102|pid:none) Methanosphaera stadtmanae DSM 3...    35   4.4
protein update 2009. 4.13
PSORT 

 psg: 0.70  gvh: 0.37  alm: 0.23  top: 0.70  tms: 0.27  mit: 0.17  mip: 0.00
 nuc: 0.16  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 36.0 %: plasma membrane
	 16.0 %: vacuolar
	 16.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for CHS348 is pla
5' end seq. ID CHS348F
5' end seq. 
>CHS348F.Seq
AGATAAAGAGTTGTCATCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAA
ACATGTCGCCAAAGAAGATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGA
GGTTGTATTTGAAGTTAAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGT
ATTTTTAATTCGGAATATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAAC
CGATTATATCACATTCTCTTATTCAATCCTTTTCATCATTGTAGAGTTTATCTCATTTTT
GGGTTCAGCATTGCATTTGAATAACTTTACCAATCCATGTACTTTTGTTTTGGTGGTAAC
ATTGGAACAAATCCTTGCCAAGAGAAGAAAGAAGCATCCNNNNNNNNNN
Length of 5' end seq. 409
3' end seq. ID CHS348Z
3' end seq. 
>CHS348Z.Seq
NNNNNNNNNNTCCATTAATTATGAGTATTTGGACTGTACCAATCGTTGTAAAAGATCCAA
TCATTTTCATTCTCGTCGGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATATA
TGATTCTTAGAGCCAAGAGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGTAG
AAGCTACTGATCTTTGGAGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATATTT
CATCATGGAGAGAAGGTTCCGCTTCAATCGTTAAACTTCTTAAAGCTAGAAAGATTAGTC
GTCATAAGTTAGCCATGTGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTGTG
AAGTTTTCCGTCAAACTAAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGGTA
AACATAAGGCTGAACAATCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAAAAATT
CAAGACTTTTCCTTCCAAATATTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAGTG
CTGTACTTAGATTTAATTGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGGTT
TCTCTTTCTCTACCTTATGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAGTN
AAAAACAATGGCCGTATGCTTCTTCATACCATGCTCACAATAT
Length of 3' end seq. 703
Connected seq. ID CHS348P
Connected seq. 
>CHS348P.Seq
AGATAAAGAGTTGTCATCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAA
ACATGTCGCCAAAGAAGATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGA
GGTTGTATTTGAAGTTAAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGT
ATTTTTAATTCGGAATATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAAC
CGATTATATCACATTCTCTTATTCAATCCTTTTCATCATTGTAGAGTTTATCTCATTTTT
GGGTTCAGCATTGCATTTGAATAACTTTACCAATCCATGTACTTTTGTTTTGGTGGTAAC
ATTGGAACAAATCCTTGCCAAGAGAAGAAAGAAGCATCC----------TCCATTAATTA
TGAGTATTTGGACTGTACCAATCGTTGTAAAAGATCCAATCATTTTCATTCTCGTCGGTA
TGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATATATGATTCTTAGAGCCAAGAGAC
CTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGTAGAAGCTACTGATCTTTGGAGAG
CTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATATTTCATCATGGAGAGAAGGTTCCG
CTTCAATCGTTAAACTTCTTAAAGCTAGAAAGATTAGTCGTCATAAGTTAGCCATGTGGA
ATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTGTGAAGTTTTCCGTCAAACTAAAT
TGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGGTAAACATAAGGCTGAACAATCTT
TCCGTACTTCAAACAAAGAATCTGATACCATTAAAAATTCAAGACTTTTCCTTCCAAATA
TTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAGTGCTGTACTTAGATTTAATTGTT
TCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGGTTTCTCTTTCTCTACCTTATGGC
ATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAGTNAAAAACAATGGCCGTATGCTT
CTTCATACCATGCTCACAATAT
Length of connected seq. 1092
Full length Seq ID -
Full length Seq. -
Length of full length seq. -