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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS347
Library CH
(Link to library)
Clone ID CHS347
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16298-1|Contig-U16523-1
Original site URL
Representative seq. ID CHS347P
(Link to Original site)
Representative DNA sequence 
>CHS347 (CHS347Q) /CSM/CH/CHS3-B/CHS347Q.Seq.d/
ACCTATTTATCTTTTAAACAAATACATAATATTTTCATTAATTAATATTTTAATATTTCC
AAATTAAAATGATCCCAAAAAAACAAAAAGGATTCAATGGTGAACCAAAATCTCATCAAG
AAATTGATAGAAGTCAATCACCATTGAAACAAAGAACCAATAATAATAATTATCAACAAC
AACCACCGCAACAACAACAAAAACAACAACCACAACAACCACANCAAAAAAAACAACANC
AAATACAACAACAAAAACAACAACCACAACAACCACAACAACCACAACAACCACAACAAC
AAAAACAACAACTACAACAACCACAACAACCACAACAATATTTCCAACCAAGACAAACTC
AAAATGCTTGGGGAAAACAAAATAACAGTATCCCACTTGAAGAACAATTTCAAGAAGTAA
AACCAATCACACAACCTCAAACACCTGAAACAACAACAACAACAACAACTACAACAACTA
CAACAACAAATACAACAAATACAAATCTATTAGTTCCAAGAATTTCAAGAAGAGATATGG
TTAAAGATTCTGACATTGAAAATGTTGCAATTGTTACAGCAAAATCAAGAACTGTTCAAG
GTAAACAAATTTCTTTAATTTCAAATTTCTTTCCAATXXXXXXXXXXAATTGCAAAGTTA
GGTTATGCACCATGGGGTCTCAATCAACAAATATTCAATGGTGTCATTCCAAAGAAAACA
ATGATCATTGGTATCGATGTTGGTCATAACTCTGATATGAGAGGTCATTCAGTAGTTGGA
TTCGTTGCAACTATCGATGATAAATTTCAAAAGTATTTCTCAAGATCTTTTGTTCAAAAG
CGTCCTGGTAAAGAAATCATTGATTCTCTTGAAGAAGCAACTAAAGAAGCAATGTCAAAA
TATTTTAGTTTCAATAATTGTTTACCAGAATTGGTTATAGTTTATCGTGATGGTGTTGGT
GATGGTATGTTAGATCTTGTCAATAGATCTGAAATTGCTGCAATGAAAAAAGGTTTTGCT
GAAACACCATCTTCTTGGGGTATAAAACCAAAGTTAATGTATGTCATTGTCAAAAAGAAT
ACAAATGCACGTTTATTTACTGCTGATCCAATTGGTAATCCTCCAGCTGGTACTTTGGTT
GACTCAAAAATTACCCATCCAAATTGGTATGATTTCTTTTTAGTCTCTCAAGTAACTAAC
AATGGTTCTGTCAATCCAACTCATTATCACGTTTTATTGGATGAACAAAAAATGCCTGCT
GATTTATTTCAATTATTTACTTTCCAAATGTGTCATCTTTATTTCAACTTTGAAAAATCT
GTTCGTGTTCCAGCAACTTGTCAATTTGCACACAAAATGGCATTTTTAATTGGTAGAACA
GTTTTCGCTGATGCTGCGCCTCAATTATCNNATAAATTATTCTTCC
sequence update 2002.10.25
Translated Amino Acid sequence 
lfif*tnt*yfh*lif*YFQIKMIPKKQKGFNGEPKSHQEIDRSQSPLKQRTNNNNYQQQ
PPQQQQKQQPQQPXQKKQXQIQQQKQQPQQPQQPQQPQQQKQQLQQPQQPQQYFQPRQTQ
NAWGKQNNSIPLEEQFQEVKPITQPQTPETTTTTTTTTTTTTNTTNTNLLVPRISRRDMV
KDSDIENVAIVTAKSRTVQGKQISLISNFFP---

---IAKLGYAPWGLNQQIFNGVIPKKTMIIGIDVGHNSDMRGHSVVGFVATIDDKFQKYF
SRSFVQKRPGKEIIDSLEEATKEAMSKYFSFNNCLPELVIVYRDGVGDGMLDLVNRSEIA
AMKKGFAETPSSWGIKPKLMYVIVKKNTNARLFTADPIGNPPAGTLVDSKITHPNWYDFF
LVSQVTNNGSVNPTHYHVLLDEQKMPADLFQLFTFQMCHLYFNFEKSVRVPATCQFAHKM
AFLIGRTVFADAAPQLSXKLFF
Translated Amino Acid sequence (All Frames) 
Frame A:
tylsfkqihnifin*yfnisklk*sqknkkdsmvnqnlikklievnhh*nkepiiiiinn
nhrnnnknnnhnnhxkknnxkynnknnnhnnhnnhnnhnnknnnynnhnnhnnisnqdkl
kmlgenkitvshlknnfkk*nqshnlkhlkqqqqqqlqqlqqqiqqiqiy*fqefqeeiw
lkiltlkmlqllqqnqelfkvnkfl*fqisfq---

---nckvrlctmgsqstniqwchskenndhwyrcws*l*yersfsswircnyr**iskvf
lkifcskasw*rnh*fs*rsn*rsnvkif*fq*lftrigysls*wcw*wyvrscq*i*nc
cnekrfc*ntiflgyktkvnvchcqkeykctfiyc*snw*ssswyfg*lknypsklv*fl
fslssn*qwfcqsnslsrfig*tknac*fisiiyfpnvsslfql*kicscssnlsictqn
gifnw*nsfr*ccasiix*iil

Frame B:
piyllnkyiifslinilifpn*ndpkktkriqw*tkissrn**ksitietknq***lstt
ttatttktttttttxkkttxntttktttttttttttttttktttttttttttifptktns
kclgktk*qypt*rtisrsktnhttsnt*nnnnnnnynnynnkynkyksissknfkkryg
*rf*h*kccncyskikncsr*tnffnfkflsn---

---IAKLGYAPWGLNQQIFNGVIPKKTMIIGIDVGHNSDMRGHSVVGFVATIDDKFQKYF
SRSFVQKRPGKEIIDSLEEATKEAMSKYFSFNNCLPELVIVYRDGVGDGMLDLVNRSEIA
AMKKGFAETPSSWGIKPKLMYVIVKKNTNARLFTADPIGNPPAGTLVDSKITHPNWYDFF
LVSQVTNNGSVNPTHYHVLLDEQKMPADLFQLFTFQMCHLYFNFEKSVRVPATCQFAHKM
AFLIGRTVFADAAPQLSXKLFF

Frame C:
lfif*tnt*yfh*lif*YFQIKMIPKKQKGFNGEPKSHQEIDRSQSPLKQRTNNNNYQQQ
PPQQQQKQQPQQPXQKKQXQIQQQKQQPQQPQQPQQPQQQKQQLQQPQQPQQYFQPRQTQ
NAWGKQNNSIPLEEQFQEVKPITQPQTPETTTTTTTTTTTTTNTTNTNLLVPRISRRDMV
KDSDIENVAIVTAKSRTVQGKQISLISNFFP---

---lqs*vmhhgvsinkysmvsfqrkq*slvsmlvitli*eviq*ldslqlsminfksis
qdllfksvlvkkslillkkqlkkqcqnilvsiivyqnwl*fivmvlvmvc*ilsidlkll
q*kkvllkhhllgv*nqs*cmslskriqmhvyllliqlvilqlvlwltqklpiqigmisf
*slk*ltmvlsiqliitfywmnkkclliyfnyllskcvifistlknlfvfqqlvnlhtkw
hf*lveqfslmlrlnyxinyss
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS347 (CHS347Q) /CSM/CH/CHS3-B/CHS347Q.Seq.d/                       1756   0.0
CHL307 (CHL307Q) /CSM/CH/CHL3-A/CHL307Q.Seq.d/                       1532   0.0
AHO381 (AHO381Q) /CSM/AH/AHO3-D/AHO381Q.Seq.d/                       1532   0.0
CHS352 (CHS352Q) /CSM/CH/CHS3-C/CHS352Q.Seq.d/                       1528   0.0
AHL458 (AHL458Q) /CSM/AH/AHL4-C/AHL458Q.Seq.d/                       1528   0.0
AHC666 (AHC666Q) /CSM/AH/AHC6-C/AHC666Q.Seq.d/                       1524   0.0
SHL722 (SHL722Q) /CSM/SH/SHL7-A/SHL722Q.Seq.d/                       1518   0.0
AHL485 (AHL485Q) /CSM/AH/AHL4-D/AHL485Q.Seq.d/                       1518   0.0
AHF283 (AHF283Q) /CSM/AH/AHF2-D/AHF283Q.Seq.d/                       1518   0.0
SSK802 (SSK802Q) /CSM/SS/SSK8-A/SSK802Q.Seq.d/                       1516   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116924|AC116924.3 Dictyostelium discoideum chromosome 2 map 6357117-6445670 strain AX4, complete sequence.   283   e-167  6
AC115682|AC115682.2 Dictyostelium discoideum chromosome 2 map 4101105-4158643 strain AX4, complete sequence.    54   4e-16  8
AC098920|AC098920.6 Rattus norvegicus clone CH230-201C23, WORKING DRAFT SEQUENCE, 5 unordered pieces.    44   0.002  5
AC069535|AC069535.15 Mus musculus chromosome 7 clone RP23-453F14, WORKING DRAFT SEQUENCE, 5 unordered pieces.    48   0.006  3
AC133735|AC133735.2 Rattus norvegicus clone CH230-162F24, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    44   0.010  4
AC084016|AC084016.12 Homo sapiens 3 BAC RP11-190F16 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    36   0.021  2
AC068350|AC068350.2 Homo sapiens chromosome 3 clone RP11-81K20 map 3, WORKING DRAFT SEQUENCE, 6 unordered pieces.    36   0.021  2
AC026767|AC026767.30 Mus musculus 7 BAC RP23-328L8 (Roswell Park Cancer Institute Mouse BAC) complete sequence.    48   0.022  4
AC171133|AC171133.1 Helobdella robusta clone CH306-1A4, WORKING DRAFT SEQUENCE, 7 unordered pieces.    40   0.029  6
AC156903|AC156903.2 Bos taurus clone CH240-60N10, *** SEQUENCING IN PROGRESS ***, 42 unordered pieces.    52   0.033  1
dna update 2005.11.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116924_18(AC116924|pid:none) Dictyostelium discoideum chromoso...   427   e-118
AB455103_1(AB455103|pid:none) Botryllus primigenus BpPiwi mRNA f...   208   4e-52
EF619343_1(EF619343|pid:none) Gallus gallus piwi-like 1 (PIWI) m...   203   1e-50
EU413991_1(EU413991|pid:none) Oryzias latipes piwi-like protein ...   202   2e-50
AY493987_1(AY493987|pid:none) Podocoryne carnea Cniwi (Cniwi) mR...   200   1e-49
AB372007_1(AB372007|pid:none) Bombyx mori BmAGO3 mRNA for AGO3, ...   199   3e-49
(Q9JMB7) RecName: Full=Piwi-like protein 1;  &AB032604_1(AB03260...   193   1e-47
AF104260_1(AF104260|pid:none) Homo sapiens HIWI mRNA, complete c...   191   4e-47
CT030247_6(CT030247|pid:none) Mouse DNA sequence from clone RP23...   191   4e-47
BC028581_1(BC028581|pid:none) Homo sapiens piwi-like 1 (Drosophi...   191   4e-47
protein update 2009. 4.13
PSORT 

 psg: 0.54  gvh: 0.01  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: nuclear
	 20.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for CHS347 is nuc
5' end seq. ID CHS347F
5' end seq. 
>CHS347F.Seq
ACCTATTTATCTTTTAAACAAATACATAATATTTTCATTAATTAATATTTTAATATTTCC
AAATTAAAATGATCCCAAAAAAACAAAAAGGATTCAATGGTGAACCAAAATCTCATCAAG
AAATTGATAGAAGTCAATCACCATTGAAACAAAGAACCAATAATAATAATTATCAACAAC
AACCACCGCAACAACAACAAAAACAACAACCACAACAACCACANCAAAAAAAACAACANC
AAATACAACAACAAAAACAACAACCACAACAACCACAACAACCACAACAACCACAACAAC
AAAAACAACAACTACAACAACCACAACAACCACAACAATATTTCCAACCAAGACAAACTC
AAAATGCTTGGGGAAAACAAAATAACAGTATCCCACTTGAAGAACAATTTCAAGAAGTAA
AACCAATCACACAACCTCAAACACCTGAAACAACAACAACAACAACAACTACAACAACTA
CAACAACAAATACAACAAATACAAATCTATTAGTTCCAAGAATTTCAAGAAGAGATATGG
TTAAAGATTCTGACATTGAAAATGTTGCAATTGTTACAGCAAAATCAAGAACTGTTCAAG
GTAAACAAATTTCTTTAATTTCAAATTTCTTTCCAATNNNNNNNNNN
Length of 5' end seq. 647
3' end seq. ID CHS347Z
3' end seq. 
>CHS347Z.Seq
NNNNNNNNNNAATTGCAAAGTTAGGTTATGCACCATGGGGTCTCAATCAACAAATATTCA
ATGGTGTCATTCCAAAGAAAACAATGATCATTGGTATCGATGTTGGTCATAACTCTGATA
TGAGAGGTCATTCAGTAGTTGGATTCGTTGCAACTATCGATGATAAATTTCAAAAGTATT
TCTCAAGATCTTTTGTTCAAAAGCGTCCTGGTAAAGAAATCATTGATTCTCTTGAAGAAG
CAACTAAAGAAGCAATGTCAAAATATTTTAGTTTCAATAATTGTTTACCAGAATTGGTTA
TAGTTTATCGTGATGGTGTTGGTGATGGTATGTTAGATCTTGTCAATAGATCTGAAATTG
CTGCAATGAAAAAAGGTTTTGCTGAAACACCATCTTCTTGGGGTATAAAACCAAAGTTAA
TGTATGTCATTGTCAAAAAGAATACAAATGCACGTTTATTTACTGCTGATCCAATTGGTA
ATCCTCCAGCTGGTACTTTGGTTGACTCAAAAATTACCCATCCAAATTGGTATGATTTCT
TTTTAGTCTCTCAAGTAACTAACAATGGTTCTGTCAATCCAACTCATTATCACGTTTTAT
TGGATGAACAAAAAATGCCTGCTGATTTATTTCAATTATTTACTTTCCAAATGTGTCATC
TTTATTTCAACTTTGAAAAATCTGTTCGTGTTCCAGCAACTTGTCAATTTGCACACAAAA
TGGCATTTTTAATTGGTAGAACAGTTTTCGCTGATGCTGCGCCTCAATTATCNNATAAAT
TATTCTTCC
Length of 3' end seq. 789
Connected seq. ID CHS347P
Connected seq. 
>CHS347P.Seq
ACCTATTTATCTTTTAAACAAATACATAATATTTTCATTAATTAATATTTTAATATTTCC
AAATTAAAATGATCCCAAAAAAACAAAAAGGATTCAATGGTGAACCAAAATCTCATCAAG
AAATTGATAGAAGTCAATCACCATTGAAACAAAGAACCAATAATAATAATTATCAACAAC
AACCACCGCAACAACAACAAAAACAACAACCACAACAACCACANCAAAAAAAACAACANC
AAATACAACAACAAAAACAACAACCACAACAACCACAACAACCACAACAACCACAACAAC
AAAAACAACAACTACAACAACCACAACAACCACAACAATATTTCCAACCAAGACAAACTC
AAAATGCTTGGGGAAAACAAAATAACAGTATCCCACTTGAAGAACAATTTCAAGAAGTAA
AACCAATCACACAACCTCAAACACCTGAAACAACAACAACAACAACAACTACAACAACTA
CAACAACAAATACAACAAATACAAATCTATTAGTTCCAAGAATTTCAAGAAGAGATATGG
TTAAAGATTCTGACATTGAAAATGTTGCAATTGTTACAGCAAAATCAAGAACTGTTCAAG
GTAAACAAATTTCTTTAATTTCAAATTTCTTTCCAAT----------AATTGCAAAGTTA
GGTTATGCACCATGGGGTCTCAATCAACAAATATTCAATGGTGTCATTCCAAAGAAAACA
ATGATCATTGGTATCGATGTTGGTCATAACTCTGATATGAGAGGTCATTCAGTAGTTGGA
TTCGTTGCAACTATCGATGATAAATTTCAAAAGTATTTCTCAAGATCTTTTGTTCAAAAG
CGTCCTGGTAAAGAAATCATTGATTCTCTTGAAGAAGCAACTAAAGAAGCAATGTCAAAA
TATTTTAGTTTCAATAATTGTTTACCAGAATTGGTTATAGTTTATCGTGATGGTGTTGGT
GATGGTATGTTAGATCTTGTCAATAGATCTGAAATTGCTGCAATGAAAAAAGGTTTTGCT
GAAACACCATCTTCTTGGGGTATAAAACCAAAGTTAATGTATGTCATTGTCAAAAAGAAT
ACAAATGCACGTTTATTTACTGCTGATCCAATTGGTAATCCTCCAGCTGGTACTTTGGTT
GACTCAAAAATTACCCATCCAAATTGGTATGATTTCTTTTTAGTCTCTCAAGTAACTAAC
AATGGTTCTGTCAATCCAACTCATTATCACGTTTTATTGGATGAACAAAAAATGCCTGCT
GATTTATTTCAATTATTTACTTTCCAAATGTGTCATCTTTATTTCAACTTTGAAAAATCT
GTTCGTGTTCCAGCAACTTGTCAATTTGCACACAAAATGGCATTTTTAATTGGTAGAACA
GTTTTCGCTGATGCTGCGCCTCAATTATCNNATAAATTATTCTTCC
Length of connected seq. 1416
Full length Seq ID -
Full length Seq. -
Length of full length seq. -