>AFH526 (AFH526Q) /CSM/AF/AFH5-B/AFH526Q.Seq.d/
XXXXXXXXXXATCTCAATTNGCAGCACCTGGCTCATTTAACGATCCTGATATGTTGGAGA
TTGGCAATGGGTTGGAGAATGCAGTTGAATATAAAAGCATGTTTTCATTATGGTCAATNA
TAGCAGCACCCTTAATAGCAGGTAATGATATAATATCAATGAGTAAAGAAGCATTGGAGA
TTTTAATCAATGAGGAGGTAATTCAAGTTAACCAAGATCCATTAGGAATTCAAGGTANTC
GTGNATATAANAATCAAAAGTTAGNAGTTTATCAAAGAACTTTGATTAATAATTCTTATG
CAATTGCTTTATTTAATAGAGGTGATACTAATTCTGATANTAGCATCANNAGNGNNATGT
TCCATTTAACAANTCATCAAAATTATAACAGTCGTGNTCCTTGGTCTCATACTGATAATG
GTAC

---SQXAAPGSFNDPDMLEIGNGLENAVEYKSMFSLWSXIAAPLIAGNDIISMSKEALEI
LINEEVIQVNQDPLGIQGXRXYXNQKLXVYQRTLINNSYAIALFNRGDTNSDXSIXXXMF
HLTXHQNYNSRXPWSHTDNG

Frame A:
---SQXAAPGSFNDPDMLEIGNGLENAVEYKSMFSLWSXIAAPLIAGNDIISMSKEALEI
LINEEVIQVNQDPLGIQGXRXYXNQKLXVYQRTLINNSYAIALFNRGDTNSDXSIXXXMF
HLTXHQNYNSRXPWSHTDNG


Frame B:
---lnxqhlahltilicwrlamgwrmqlnikacfhygqx*qhp**qvmi*yq*vkkhwrf
*smrr*fkltkih*efkvxvxixiks*xfikel*liilmqllylievililixasxxxcs
i*qxikiitvvxlglilimv


Frame C:
---sixstwli*rs*yvgdwqwvgecs*i*khvfimvnxsstlnsr**ynine*rsigdf
nq*ggnss*prsirnsrxsxi*xskvxslsknfd**flcncfi**r*y*f*x*hxxxxvp
fnxsskl*qsxslvsy**wy

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH526 (AFH526Q) /CSM/AF/AFH5-B/AFH526Q.Seq.d/                        737   0.0
SSM530 (SSM530Q) /CSM/SS/SSM5-B/SSM530Q.Seq.d/                        613   e-175
VSG373 (VSG373Q) /CSM/VS/VSG3-D/VSG373Q.Seq.d/                         36   0.41
SLH819 (SLH819Q) /CSM/SL/SLH8-A/SLH819Q.Seq.d/                         36   0.41
SHI319 (SHI319Q) /CSM/SH/SHI3-A/SHI319Q.Seq.d/                         36   0.41
SHF231 (SHF231Q) /CSM/SH/SHF2-B/SHF231Q.Seq.d/                         36   0.41
CHN692 (CHN692Q) /CSM/CH/CHN6-D/CHN692Q.Seq.d/                         36   0.41
AHQ759 (AHQ759Q) /CSM/AH/AHQ7-C/AHQ759Q.Seq.d/                         36   0.41
AHM173 (AHM173Q) /CSM/AH/AHM1-D/AHM173Q.Seq.d/                         36   0.41
AHJ405 (AHJ405Q) /CSM/AH/AHJ4-A/AHJ405Q.Seq.d/                         36   0.41

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AU298945|AU298945.1 Cryptomeria japonica mRNA, clone: CM0198, 3' end, expressed in male cone.    52   0.007  1
AC015612|AC015612.2 Homo sapiens clone RP11-45G3, LOW-PASS SEQUENCE SAMPLING.    48   0.10   1
AC012099|AC012099.4 Homo sapiens chromosome 14 clone RP11-45G3 containing neurexin III gene, partial cds, complete sequence.    48   0.10   1
AC106855|AC106855.2 Homo sapiens chromosome 18 clone RP11-51G16 map 18, LOW-PASS SEQUENCE SAMPLING.    48   0.10   1
CF573573|CF573573.1 MCSA071H01 Maturing Sugarcane Stem Lambda ZIPLOX Library (MCS) Saccharum sp. cDNA clone MCSA094G03 5' similar to alpha galactosidase, mRNA sequence.    48   0.10   1
BJ573992|BJ573992.1 Ipomoea nil cDNA clone:jm25h24, 3' end, single read.    44   0.14   2
CF210099|CF210099.1 CAB20005_Ia_Ra_A07 Cabernet Sauvignon Flower bloom - CAB2 Vitis vinifera cDNA clone CAB20005_Ia_Ra_A07 3', mRNA sequence.    44   0.15   2
BJ569024|BJ569024.1 Ipomoea nil cDNA clone:jm4f21, 3' end, single read.    44   0.15   2
BJ573040|BJ573040.1 Ipomoea nil cDNA clone:jm22k05, 3' end, single read.    44   0.15   2
CB348691|CB348691.1 CAB2SG0005_IIaR_C02 Cabernet Sauvignon Berry - CAB2SG Vitis vinifera cDNA clone CAB2SG0005_IIaR_C02 3', mRNA sequence.    44   0.16   2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000360_1673(CP000360|pid:none) Acidobacteria bacterium Ellin34...   114   8e-25
EU961965_1(EU961965|pid:none) Zea mays clone 239357 unknown mRNA.     109   3e-23
AL939104_273(AL939104|pid:none) Streptomyces coelicolor A3(2) co...   107   9e-23
AY738151_1(AY738151|pid:none) Salvia miltiorrhiza glycosyl hydro...   107   2e-22
CP001348_1205(CP001348|pid:none) Clostridium cellulolyticum H10,...   106   2e-22
AK317201_1(AK317201|pid:none) Arabidopsis thaliana AT5G08370 mRN...   105   4e-22
AY085529_1(AY085529|pid:none) Arabidopsis thaliana clone 156141 ...   105   4e-22
AB025362_1(AB025362|pid:none) Clostridium josui agaA gene for al...   105   4e-22
EF085713_1(EF085713|pid:none) Picea sitchensis clone WS0294_N01 ...   105   6e-22
CP001348_1561(CP001348|pid:none) Clostridium cellulolyticum H10,...   104   8e-22

 psg: 0.75  gvh: 0.56  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for AFH526 is cyt

>AFH526Z.Seq
----------ATCTCAATTNGCAGCACCTGGCTCATTTAACGATCCTGATATGTTGGAGA
TTGGCAATGGGTTGGAGAATGCAGTTGAATATAAAAGCATGTTTTCATTATGGTCAATNA
TAGCAGCACCCTTAATAGCAGGTAATGATATAATATCAATGAGTAAAGAAGCATTGGAGA
TTTTAATCAATGAGGAGGTAATTCAAGTTAACCAAGATCCATTAGGAATTCAAGGTANTC
GTGNATATAANAATCAAAAGTTAGNAGTTTATCAAAGAACTTTGATTAATAATTCTTATG
CAATTGCTTTATTTAATAGAGGTGATACTAATTCTGATANTAGCATCANNAGNGNNATGT
TCCATTTAACAANTCATCAAAATTATAACAGTCGTGNTCCTTGGTCTCATACTGATAATG
GTAC