>CHS346 (CHS346Q) /CSM/CH/CHS3-B/CHS346Q.Seq.d/
GAAAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATATATATTAATAT
ATTAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATTTTA
AATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGA
AAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGA
AATGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGT
TATTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAA
TTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGT
CCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTT
TTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCA
GGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAA
CCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACT
GGTAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGXXXXXXXXXXTGGAGAGTG
GAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATCACTCACTGGTCTTA
GATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCATGACTATA
CCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGGTTTCGATT
ACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAAACAACTAA
AAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAACCTTTGGTA
AACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACAATATCGTG
TTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGAACCTGGTA
TTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAGTCAAACTG
TATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTATTATTGTG
TAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGCTGATCCAA
CTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTGATAATGTTTATGTCACTTC
TTTTACTTCTTC

ekf*llyiiflniyiniy*YININKMKILYSLLLISSIILNTVLNISSQVYDQRILALKG
KCYLDSNKNALYDEGEPGFGNVEISLGNATDYKGNKYPIRYSSADGTFIYDGLTQRTTYE
FTFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGVSS
GSSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPPPFTGSAVAGLLFTDIN---

---ESGGKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIV
TTGFDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSS
LPQYRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFS
SNYYCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVIMFMSLLLLL

Frame A:
ekf*llyiiflniyiniy*YININKMKILYSLLLISSIILNTVLNISSQVYDQRILALKG
KCYLDSNKNALYDEGEPGFGNVEISLGNATDYKGNKYPIRYSSADGTFIYDGLTQRTTYE
FTFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGVSS
GSSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPPPFTGSAVAGLLFTDIN---

---wrvevnvvvllvpeliknhslvldmvqvmvisliiikqvfmtipmllvqlkvvvill
lqlvsitmkhlkvqfvgimqplvnn*kvtlyi*plqmvvphlqplvnkmvlvillqcmil
lfhnivlvefgli*mvmvfkmlvnlvfqesqys*smvnnqlqqvklypiqmvisnlklis
vqiiiv*fllhssklvlplq*emliqliqiqivmlsyql**clchffyff

Frame B:
knfnyci*yf*iyiliyinililik*kyyihyy*yqv*f*ily*ifhhkyminvfwh*ke
nvi*iqikmhcmmkvnqdlemlkfl*emqliireiniqyvihllmvhlymmd*lnvqlmn
lhlpiqkiitlkpmkrhwlvhqvyhvfvqrfqliiwqqif*lqiplpitfh*lnklvfhq
ahlylqlelvliwvmlsklnhqlnhllnhllnhqhlhhllvvpslvyylpism---

---gewr*mw*scwcrs*skithws*iwsr*w*fl**s*nrss*lyqcfwcn*rws*yyc
ynwfrll*si*rfnslv*cnlw*ttkrllfiynlfkwsypisnlw*tkwsw*yyfsv*ff
sstiscw*sla*ykw*wysrcw*twyfrnhsilnlwstinsnksncirfkwlfqi*s*fq
fklllcnsccilpswffhynekc*snsskyk**cflincdnvyvtsfts

Frame C:
kiliivynifkyiy*yiliy*y**neniifiiinikynfkycikyfitsi*styfgikrk
mlfrfk*kciv**r*triwkc*nffrkcn*l*gk*isntlfic*wyiyi*winstynl*i
yiyqskrlll*nl*kdiglstrctmyssngfs**fgsrsfsckylcl*hsid*tswcfir
liytys*n*c*fglc*ansttnsttysttysttntstiyw*crrwfiiyryqw---

---ESGGKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIV
TTGFDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSS
LPQYRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFS
SNYYCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVIMFMSLLLLL

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS346 (CHS346Q) /CSM/CH/CHS3-B/CHS346Q.Seq.d/                       2454   0.0
SHG134 (SHG134Q) /CSM/SH/SHG1-B/SHG134Q.Seq.d/                       1350   0.0
CHG402 (CHG402Q) /CSM/CH/CHG4-A/CHG402Q.Seq.d/                       1344   0.0
SHJ741 (SHJ741Q) /CSM/SH/SHJ7-B/SHJ741Q.Seq.d/                       1342   0.0
AHO147 (AHO147Q) /CSM/AH/AHO1-B/AHO147Q.Seq.d/                       1342   0.0
SHG818 (SHG818Q) /CSM/SH/SHG8-A/SHG818Q.Seq.d/                       1340   0.0
SHK374 (SHK374Q) /CSM/SH/SHK3-D/SHK374Q.Seq.d/                       1338   0.0
CHQ382 (CHQ382Q) /CSM/CH/CHQ3-D/CHQ382Q.Seq.d/                       1338   0.0
AHF465 (AHF465Q) /CSM/AH/AHF4-C/AHF465Q.Seq.d/                       1338   0.0
SSF392 (SSF392Q) /CSM/SS/SSF3-D/SSF392Q.Seq.d/                       1336   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   3e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   3e-05  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   3e-05  1
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   4e-05  3
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   5e-04  3
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   5e-04  1
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   5e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   5e-04  1
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.002  3
DT775356|DT775356.1 124719991 TH1 Tribolium castaneum cDNA clone 3C7 3', mRNA sequence.    54   0.008  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000686_3542(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    80   2e-13
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    63   2e-08
CP000910_1528(CP000910|pid:none) Renibacterium salmoninarum ATCC...    59   4e-07
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    59   4e-07
(Q7A780) RecName: Full=Serine-aspartate repeat-containing protei...    53   3e-05
AM076203_1(AM076203|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076200_1(AM076200|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076196_1(AM076196|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076198_1(AM076198|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
CP000703_575(CP000703|pid:none) Staphylococcus aureus subsp. aur...    53   3e-05

 psg: 0.95  gvh: 0.59  alm: 0.26  top: 0.40  tms: 0.13  mit: 0.31  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 28.0 %: endoplasmic reticulum
	 16.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: cytoplasmic
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for CHS346 is mit

>CHS346F.Seq
GAAAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATATATATTAATAT
ATTAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATTTTA
AATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGA
AAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGA
AATGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGT
TATTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAA
TTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGT
CCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTT
TTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCA
GGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAA
CCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACT
GGTAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGNNNNNNNNNN

>CHS346Z.Seq
NNNNNNNNNNTGGAGAGTGGAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAA
AAATCACTCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAA
ACAGGTCTTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATT
GTTACAACTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCA
ACCTCTGGTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACC
CCATCTCCAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCT
TCTCTTCCACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAA
GATGCTGGTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCA
ACTCCAACAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTC
AGTTCAAATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACA
ATGAGAAATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTG
ATAATGTTTATGTCACTTCTTTTACTTCTTC

>CHS346P.Seq
GAAAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATATATATTAATAT
ATTAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATTTTA
AATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGA
AAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGA
AATGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGT
TATTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAA
TTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGT
CCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTT
TTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCA
GGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAA
CCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACT
GGTAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGG----------TGGAGAGTG
GAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATCACTCACTGGTCTTA
GATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCATGACTATA
CCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGGTTTCGATT
ACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAAACAACTAA
AAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAACCTTTGGTA
AACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACAATATCGTG
TTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGAACCTGGTA
TTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAGTCAAACTG
TATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTATTATTGTG
TAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGCTGATCCAA
CTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTGATAATGTTTATGTCACTTC
TTTTACTTCTTC