>CHS333 (CHS333Q) /CSM/CH/CHS3-B/CHS333Q.Seq.d/
GTCAAAATTCCTTTTATTTATAAATAAACCAAAAAACAGGATATTTGTTATTTTATAATG
AGTAAAAGAAAGCACGAATCATCAGATTCAAATAAAAAAGCAATGAAAAAACAACAAAAT
AAAATAGAAGAAGAAGAAGAAGAAATTACAAATACTACAACTACAACTACAACCACAAAT
AATAATTATAATAATATAAAATCAAATTTAACAATTGATCAATGGATACCAAAAAAAGAA
ACATTTTCAAAACGTTATTATGAAATTTTAGAAAAGAGAAAAGAGTTACCAGTTTGGAAA
CAAAAAGAGGATTTTATAAAAGTTATTAAAGAGAATCAAGTTGTAATTTTAGTTGGTGAA
ACAGGTTCAGGTAAAACCACACAAATTCCACAATTTGTAGTTGATGCAGGATTAATTAGA
CCAGGTAAAATGGTAGGTGTTACACAACCAAGAAGAGTAGCAGCAATAAGTGTAGCAAAA
AGAGTATCAGAGGAAATGGATTTTGAATTGGGTGAAGAGGTTGGTTATTCAATTCGTTTC
XXXXXXXXXXGATAGTTTCAGCTGAACGTAGTTGGTCCAATGAAATCTTAACAATTGCTG
CAATGTTATCCGCTCCAAATTGTTTTATGCGTCCAAAGGATAACAGAATCGAAGCTGACT
CTGCAAAGAAATCATTTGATCATTTCGATGGTGATCATCTTACAATGTTAAATGTTTATC
ATAGTTTTAAAAAGAATGGTGAAGATCCAACTTGGTGTTACGATAATTTCCTCAATCATC
GTGCCATTAAACAAGCCGATAGTGTCCGTTCACAATTGGCTAGAATCTTAACTCGTTTCA
AATTACCATTGGTTAGTGGTGATGTAAATAGTAAATTTTATTATGAAAATATAAAGAAAT
GTATCGCTGCTGGCTTCTTTATGCAAGTGGCTAAATGCGAGAAAAAAAATATCTACTTCA
CCCTAGGTGATGAACAATCTGTAATCTTTCATCCTTCAACTGGTTTAACTCGTCGTCCTG
AATTTTGTATCTACAATGAATTTGTTTTAACCTCTGAAAATTATATTAGAACAATCACTG
ATGTTAAATTTGATTGGTTATTAGAATTGGCTCCAAGTTATTTTAAACAAAAATCTTTCC
CTAAAAAAACAAAAGAAACAATTCAAAGAGCTCAAAGATTATATAGTGGTAGTAGTAGTG
GTAGTAGTAGTGGTAGTAATAAAAAATAAATAAATTAATAAATTTTAAAAATAAAATAAA
ATATTTNATTAATATATTAAATNTTTAAAAA

vkipfiyk*TKKQDICYFIMSKRKHESSDSNKKAMKKQQNKIEEEEEEITNTTTTTTTTN
NNYNNIKSNLTIDQWIPKKETFSKRYYEILEKRKELPVWKQKEDFIKVIKENQVVILVGE
TGSGKTTQIPQFVVDAGLIRPGKMVGVTQPRRVAAISVAKRVSEEMDFELGEEVGYSIRF
---

---IVSAERSWSNEILTIAAMLSAPNCFMRPKDNRIEADSAKKSFDHFDGDHLTMLNVYH
SFKKNGEDPTWCYDNFLNHRAIKQADSVRSQLARILTRFKLPLVSGDVNSKFYYENIKKC
IAAGFFMQVAKCEKKNIYFTLGDEQSVIFHPSTGLTRRPEFCIYNEFVLTSENYIRTITD
VKFDWLLELAPSYFKQKSFPKKTKETIQRAQRLYSGSSSGSSSGSNKK*in**ilkik*n
ixliy*xfk

Frame A:
vkipfiyk*TKKQDICYFIMSKRKHESSDSNKKAMKKQQNKIEEEEEEITNTTTTTTTTN
NNYNNIKSNLTIDQWIPKKETFSKRYYEILEKRKELPVWKQKEDFIKVIKENQVVILVGE
TGSGKTTQIPQFVVDAGLIRPGKMVGVTQPRRVAAISVAKRVSEEMDFELGEEVGYSIRF
---

---dsfs*t*lvq*nlnnccnvirsklfyaskg*qnrs*lckeii*sfrw*ssynvkcls
*f*kew*rsnlvlr*fpqssch*tsr*cpftig*nlnsfqitig*w*ck**ill*kykem
yrcwllyasg*mrekkyllhpr**ticnlssfnwfnsss*ilylq*icfnl*kly*nnh*
c*i*lvirigsklf*tkifp*knkrnnsksskii*w***w***w***kinklinfknkik
yxinilnx*k

Frame B:
skfllfinkpknrifvil**vkestnhqiqikkq*knnkik*kkkkkklqilqlqlqpqi
iiiii*nqi*qlingyqkkkhfqnvimkf*kreksyqfgnkkril*kllkrikl*f*lvk
qvqvkphkfhnl*lmqd*ldqvkw*vlhnqee*qq*v*qkeyqrkwilnwvkrlviqfv-
--

---IVSAERSWSNEILTIAAMLSAPNCFMRPKDNRIEADSAKKSFDHFDGDHLTMLNVYH
SFKKNGEDPTWCYDNFLNHRAIKQADSVRSQLARILTRFKLPLVSGDVNSKFYYENIKKC
IAAGFFMQVAKCEKKNIYFTLGDEQSVIFHPSTGLTRRPEFCIYNEFVLTSENYIRTITD
VKFDWLLELAPSYFKQKSFPKKTKETIQRAQRLYSGSSSGSSSGSNKK*in**ilkik*n
ixliy*xfk

Frame C:
qnsfyl*inqktgyllfyne*kkariirfk*ksnekttk*nrrrrrrnykyynynynhk*
*l**ykikfnn*smdtkkrnifktll*nfrkekrvtsletkrgfyksy*resscnfsw*n
rfr*nhtnstics*crin*tr*ngrcyttkksssnkcskksirgngf*ig*rgwlfnsf-
--

---*fqlnvvgpmks*qllqcyplqivlcvqriteskltlqrnhliismviilqc*mfii
vlkrmvkiqlgvtiissiivplnkpivsvhnwles*lvsnyhwlvvm*ivnfimki*rnv
sllaslckwlnarkkistsp*vmnnl*sfilqlv*lvvlnfvstmnlf*plkiileqslm
lnligy*nwlqvilnknlslkkqkkqfkelkdyivvvvvvvvvvviknk*inkf*k*nki
fx*yikxlk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS333 (CHS333Q) /CSM/CH/CHS3-B/CHS333Q.Seq.d/                       1869   0.0
CHI155 (CHI155Q) /CSM/CH/CHI1-C/CHI155Q.Seq.d/                       1166   0.0
CHR355 (CHR355Q) /CSM/CH/CHR3-C/CHR355Q.Seq.d/                       1158   0.0
CHI803 (CHI803Q) /CSM/CH/CHI8-A/CHI803Q.Seq.d/                       1158   0.0
CHI582 (CHI582Q) /CSM/CH/CHI5-D/CHI582Q.Seq.d/                       1158   0.0
CHI233 (CHI233Q) /CSM/CH/CHI2-B/CHI233Q.Seq.d/                       1158   0.0
AHR238 (AHR238Q) /CSM/AH/AHR2-B/AHR238Q.Seq.d/                       1158   0.0
CHF648 (CHF648Q) /CSM/CH/CHF6-B/CHF648Q.Seq.d/                       1142   0.0
VHD813 (VHD813Q) /CSM/VH/VHD8-A/VHD813Q.Seq.d/                       1138   0.0
CHF541 (CHF541Q) /CSM/CH/CHF5-B/CHF541Q.Seq.d/                       1116   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Z72642|Z72642.1 S.cerevisiae chromosome VII reading frame ORF YGL120c.    66   1e-09  3
U41851|U41851.1 Saccharomyces cerevisiae splicing factor Prp43p (PRP43) gene, complete cds.    66   1e-09  3
AX489029|AX489029.1 Sequence 6329 from Patent WO02053728.          48   1e-08  4
AX830412|AX830412.1 Sequence 1132 from Patent WO03072602.          66   1e-08  2
AX819382|AX819382.1 Sequence 1132 from Patent EP1338608.           66   1e-08  2
AX595292|AX595292.1 Sequence 946 from Patent EP1258494.            66   1e-08  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    70   1e-07  1
AL929357|AL929357.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 3/5.    52   5e-06  2
AL397511|AL397511.1 T3 end of clone AS0AA003D12 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.    62   3e-05  1
AF076279|AF076279.1 Dictyostelium firmibasis plasmid Dfp1, complete plasmid sequence.    60   9e-05  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NJ4) RecName: Full=Putative pre-mRNA-splicing factor ATP-dep...   432   e-119
BT034008_1(BT034008|pid:none) Zea mays full-length cDNA clone ZM...   246   2e-63
EU961300_1(EU961300|pid:none) Zea mays clone 234415 pre-mRNA-spl...   243   8e-63
AC153352_12(AC153352|pid:none) Medicago truncatula clone mth2-10...   243   1e-62
(Q20875) RecName: Full=Putative pre-mRNA-splicing factor ATP-dep...   243   1e-62
CR954201_307(CR954201|pid:none) Ostreococcus tauri strain OTTH05...   239   1e-61
AL162507_1(AL162507|pid:none) Arabidopsis thaliana DNA chromosom...   239   1e-61
(O22899) RecName: Full=Probable pre-mRNA-splicing factor ATP-dep...   237   7e-61
AB001636_1(AB001636|pid:none) Homo sapiens mRNA for ATP-dependen...   230   7e-59
AK303506_1(AK303506|pid:none) Homo sapiens cDNA FLJ55635 complet...   230   7e-59

 psg: 0.82  gvh: 0.26  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.14  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for CHS333 is cyt

>CHS333F.Seq
GTCAAAATTCCTTTTATTTATAAATAAACCAAAAAACAGGATATTTGTTATTTTATAATG
AGTAAAAGAAAGCACGAATCATCAGATTCAAATAAAAAAGCAATGAAAAAACAACAAAAT
AAAATAGAAGAAGAAGAAGAAGAAATTACAAATACTACAACTACAACTACAACCACAAAT
AATAATTATAATAATATAAAATCAAATTTAACAATTGATCAATGGATACCAAAAAAAGAA
ACATTTTCAAAACGTTATTATGAAATTTTAGAAAAGAGAAAAGAGTTACCAGTTTGGAAA
CAAAAAGAGGATTTTATAAAAGTTATTAAAGAGAATCAAGTTGTAATTTTAGTTGGTGAA
ACAGGTTCAGGTAAAACCACACAAATTCCACAATTTGTAGTTGATGCAGGATTAATTAGA
CCAGGTAAAATGGTAGGTGTTACACAACCAAGAAGAGTAGCAGCAATAAGTGTAGCAAAA
AGAGTATCAGAGGAAATGGATTTTGAATTGGGTGAAGAGGTTGGTTATTCAATTCGTTTC
NNNNNNNNNN

>CHS333Z.Seq
NNNNNNNNNNGATAGTTTCAGCTGAACGTAGTTGGTCCAATGAAATCTTAACAATTGCTG
CAATGTTATCCGCTCCAAATTGTTTTATGCGTCCAAAGGATAACAGAATCGAAGCTGACT
CTGCAAAGAAATCATTTGATCATTTCGATGGTGATCATCTTACAATGTTAAATGTTTATC
ATAGTTTTAAAAAGAATGGTGAAGATCCAACTTGGTGTTACGATAATTTCCTCAATCATC
GTGCCATTAAACAAGCCGATAGTGTCCGTTCACAATTGGCTAGAATCTTAACTCGTTTCA
AATTACCATTGGTTAGTGGTGATGTAAATAGTAAATTTTATTATGAAAATATAAAGAAAT
GTATCGCTGCTGGCTTCTTTATGCAAGTGGCTAAATGCGAGAAAAAAAATATCTACTTCA
CCCTAGGTGATGAACAATCTGTAATCTTTCATCCTTCAACTGGTTTAACTCGTCGTCCTG
AATTTTGTATCTACAATGAATTTGTTTTAACCTCTGAAAATTATATTAGAACAATCACTG
ATGTTAAATTTGATTGGTTATTAGAATTGGCTCCAAGTTATTTTAAACAAAAATCTTTCC
CTAAAAAAACAAAAGAAACAATTCAAAGAGCTCAAAGATTATATAGTGGTAGTAGTAGTG
GTAGTAGTAGTGGTAGTAATAAAAAATAAATAAATTAATAAATTTTAAAAATAAAATAAA
ATATTTNATTAATATATTAAATNTTTAAAAA

>CHS333P.Seq
GTCAAAATTCCTTTTATTTATAAATAAACCAAAAAACAGGATATTTGTTATTTTATAATG
AGTAAAAGAAAGCACGAATCATCAGATTCAAATAAAAAAGCAATGAAAAAACAACAAAAT
AAAATAGAAGAAGAAGAAGAAGAAATTACAAATACTACAACTACAACTACAACCACAAAT
AATAATTATAATAATATAAAATCAAATTTAACAATTGATCAATGGATACCAAAAAAAGAA
ACATTTTCAAAACGTTATTATGAAATTTTAGAAAAGAGAAAAGAGTTACCAGTTTGGAAA
CAAAAAGAGGATTTTATAAAAGTTATTAAAGAGAATCAAGTTGTAATTTTAGTTGGTGAA
ACAGGTTCAGGTAAAACCACACAAATTCCACAATTTGTAGTTGATGCAGGATTAATTAGA
CCAGGTAAAATGGTAGGTGTTACACAACCAAGAAGAGTAGCAGCAATAAGTGTAGCAAAA
AGAGTATCAGAGGAAATGGATTTTGAATTGGGTGAAGAGGTTGGTTATTCAATTCGTTTC
----------GATAGTTTCAGCTGAACGTAGTTGGTCCAATGAAATCTTAACAATTGCTG
CAATGTTATCCGCTCCAAATTGTTTTATGCGTCCAAAGGATAACAGAATCGAAGCTGACT
CTGCAAAGAAATCATTTGATCATTTCGATGGTGATCATCTTACAATGTTAAATGTTTATC
ATAGTTTTAAAAAGAATGGTGAAGATCCAACTTGGTGTTACGATAATTTCCTCAATCATC
GTGCCATTAAACAAGCCGATAGTGTCCGTTCACAATTGGCTAGAATCTTAACTCGTTTCA
AATTACCATTGGTTAGTGGTGATGTAAATAGTAAATTTTATTATGAAAATATAAAGAAAT
GTATCGCTGCTGGCTTCTTTATGCAAGTGGCTAAATGCGAGAAAAAAAATATCTACTTCA
CCCTAGGTGATGAACAATCTGTAATCTTTCATCCTTCAACTGGTTTAACTCGTCGTCCTG
AATTTTGTATCTACAATGAATTTGTTTTAACCTCTGAAAATTATATTAGAACAATCACTG
ATGTTAAATTTGATTGGTTATTAGAATTGGCTCCAAGTTATTTTAAACAAAAATCTTTCC
CTAAAAAAACAAAAGAAACAATTCAAAGAGCTCAAAGATTATATAGTGGTAGTAGTAGTG
GTAGTAGTAGTGGTAGTAATAAAAAATAAATAAATTAATAAATTTTAAAAATAAAATAAA
ATATTTNATTAATATATTAAATNTTTAAAAA