>CHS321 (CHS321Q) /CSM/CH/CHS3-A/CHS321Q.Seq.d/
XXXXXXXXXXATGTCATGGATTCAGCTAAAGGTTGGGCAGGCTCAATTCGTTTCAATCAA
CAAGTTTCAAAACAATTTGATCATTTNTATGGTAGAAACGATACTTTTTCATTGGGTNTT
TGCAATGGTTGCCAATTGATGGCTTTGCTTGGCTGGGTGCCATATAGAGGTATTGAACAA
ACTCATCAACCTCGTTTCATTCATAACGCATCCGGTCGTTTCGAATCTCGTTGGGTCAAT
GTTAAAATTATGCCATCACCTGCATTACTCTTGAAAGGTATGGAAGGCTCAGTACTTGGT
GTTTGGTCTCAACATGGTGAAGGTCGTTTNTGGAGTGAAGATCAATCAATTGTCAATGAT
ATCAAAGCTAACAATCTNTCACCAATTCGTTATGTCGATGATGATGGTGAAATCACTGAA
TCCTATCCATTCAATCCATCTGGTACTCAANAGGGTTTCGCTTCCTTATGCTCAAAAGAT
GGTAGACATTTAGCAATTATGCCACATCCAGAACGTTCTTTCCTCTCTTGGCAATGGCCA
TTCATGCCTGAAAATATTAAACAAAATGTTGGTGGTTTAGATCAACCATCACCTTGGATT
AAAATTTTCCAAAATGNTAAATCATTTTGAGATTCTTTAAATTAATTTTTTTAAAAAAAA
AACAAAAACAAACAAAAAAATAAATAAATAAATAAAAAATTTAAATAAATAAAATT

---VMDSAKGWAGSIRFNQQVSKQFDHXYGRNDTFSLGXCNGCQLMALLGWVPYRGIEQT
HQPRFIHNASGRFESRWVNVKIMPSPALLLKGMEGSVLGVWSQHGEGRXWSEDQSIVNDI
KANNLSPIRYVDDDGEITESYPFNPSGTQXGFASLCSKDGRHLAIMPHPERSFLSWQWPF
MPENIKQNVGGLDQPSPWIKIFQNXKSF*dsln*ff*kknknkqknk*inkkfk*ik

Frame A:
---chgfs*rlgrlnsfqstsfkti*sfxw*kryffigxlqwlpidgfawlgai*ry*tn
sstsfhs*rirsfrislgqc*nyaitcitlerygrlstwclvstw*rsfxe*rsincq*y
qs*qsxtnslcr**w*nh*ilsiqsiwysxgfrflmlkrw*tfsnyatsrtffpllamai
ha*ky*tkcwwfrstitld*nfpkx*iilrffkliflkkkqkqtkk*ink*ki*inki


Frame B:
---VMDSAKGWAGSIRFNQQVSKQFDHXYGRNDTFSLGXCNGCQLMALLGWVPYRGIEQT
HQPRFIHNASGRFESRWVNVKIMPSPALLLKGMEGSVLGVWSQHGEGRXWSEDQSIVNDI
KANNLSPIRYVDDDGEITESYPFNPSGTQXGFASLCSKDGRHLAIMPHPERSFLSWQWPF
MPENIKQNVGGLDQPSPWIKIFQNXKSF*dsln*ff*kknknkqknk*inkkfk*ik


Frame C:
---swiqlkvgqaqfvsinkfqnnliixmvetilfhwvfamvan*wlclagchievlnkl
inlvsfithpvvsnlvgsmlklchhlhys*kvwkaqylvfglnmvkvvxgvkinqlsmis
kltixhqfvmsmmmvkslnpihsihlvlxrvslpyaqkmvdi*qlchiqnvlsslgnghs
clkilnkmlvv*inhhlglkfskmxnhfeil*inffkkktktnkkink*iknlnk*n

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS321 (CHS321Q) /CSM/CH/CHS3-A/CHS321Q.Seq.d/                       1235   0.0
VHN391 (VHN391Q) /CSM/VH/VHN3-D/VHN391Q.Seq.d/                       1193   0.0
CHK287 (CHK287Q) /CSM/CH/CHK2-D/CHK287Q.Seq.d/                        797   0.0
SSE152 (SSE152Q) /CSM/SS/SSE1-C/SSE152Q.Seq.d/                         40   0.045
SSD578 (SSD578Q) /CSM/SS/SSD5-D/SSD578Q.Seq.d/                         40   0.045
SSA889 (SSA889Q) /CSM/SS/SSA8-D/SSA889Q.Seq.d/                         40   0.045
VHP484 (VHP484Q) /CSM/VH/VHP4-D/VHP484Q.Seq.d/                         38   0.18
VHI482 (VHI482Q) /CSM/VH/VHI4-D/VHI482Q.Seq.d/                         38   0.18
CHR636 (CHR636Q) /CSM/CH/CHR6-B/CHR636Q.Seq.d/                         38   0.18
CHM244 (CHM244Q) /CSM/CH/CHM2-B/CHM244Q.Seq.d/                         38   0.18

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BZ960774|BZ960774.1 PUGII53TD ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa391I09,genomic survey sequence.    38   1e-04  3
CK269381|CK269381.1 EST715459 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POACM48 3' end, mRNA sequence.    46   0.021  2
DV626238|DV626238.1 96676.1 Cold Sweetening C Solanum tuberosum cDNA clone 96676 5', mRNA sequence.    46   0.033  2
CA281240|CA281240.1 SCRFSD1023C12.g SD1 Saccharum officinarum cDNA clone SCRFSD1023C12 5', mRNA sequence.    32   0.043  3
CK278044|CK278044.1 EST724122 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAE360 3' end, mRNA sequence.    46   0.049  2
CK269380|CK269380.1 EST715458 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POACM48 5' end, mRNA sequence.    46   0.050  2
CK276419|CK276419.1 EST722497 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POADT38 5' end, mRNA sequence.    46   0.053  2
U58747|U58747.3 Caenorhabditis elegans cosmid C55F2, complete sequence.    40   0.78   2
DN939934|DN939934.1 7688.3 After-Cooking Darkening A Solanum tuberosum cDNA clone 7688 3', mRNA sequence.    46   1.0    1
CX661134|CX661134.1 V01006C10_557698 Fragaria vesca heat stressed seedlings cDNA library in pCMV-SPORT 6.1 Fragaria vesca cDNA clone V01006C10 5, mRNA sequence.    46   1.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JC8) RecName: Full=Phosphoribosylformylglycinamidine synthas...   430   e-119
CP001147_618(CP001147|pid:none) Thermodesulfovibrio yellowstonii...   270   4e-71
BT034358_1(BT034358|pid:none) Zea mays full-length cDNA clone ZM...   256   4e-67
EU091297_1(EU091297|pid:none) Arabidopsis thaliana phosphoribosy...   256   5e-67
AP008207_3307(AP008207|pid:none) Oryza sativa (japonica cultivar...   253   5e-66
CP001575_99(CP001575|pid:none) Micromonas sp. RCC299 chromosome ...   224   2e-57
AK172938_1(AK172938|pid:none) Mus musculus mRNA for mKIAA0361 pr...   206   6e-52
BC114997_1(BC114997|pid:none) Mus musculus phosphoribosylformylg...   206   6e-52
BC063538_1(BC063538|pid:none) Homo sapiens phosphoribosylformylg...   197   3e-49
AK295895_1(AK295895|pid:none) Homo sapiens cDNA FLJ61288 complet...   197   3e-49

 psg: 0.76  gvh: 0.40  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHS321 is cyt

>CHS321Z.Seq
NNNNNNNNNNATGTCATGGATTCAGCTAAAGGTTGGGCAGGCTCAATTCGTTTCAATCAA
CAAGTTTCAAAACAATTTGATCATTTNTATGGTAGAAACGATACTTTTTCATTGGGTNTT
TGCAATGGTTGCCAATTGATGGCTTTGCTTGGCTGGGTGCCATATAGAGGTATTGAACAA
ACTCATCAACCTCGTTTCATTCATAACGCATCCGGTCGTTTCGAATCTCGTTGGGTCAAT
GTTAAAATTATGCCATCACCTGCATTACTCTTGAAAGGTATGGAAGGCTCAGTACTTGGT
GTTTGGTCTCAACATGGTGAAGGTCGTTTNTGGAGTGAAGATCAATCAATTGTCAATGAT
ATCAAAGCTAACAATCTNTCACCAATTCGTTATGTCGATGATGATGGTGAAATCACTGAA
TCCTATCCATTCAATCCATCTGGTACTCAANAGGGTTTCGCTTCCTTATGCTCAAAAGAT
GGTAGACATTTAGCAATTATGCCACATCCAGAACGTTCTTTCCTCTCTTGGCAATGGCCA
TTCATGCCTGAAAATATTAAACAAAATGTTGGTGGTTTAGATCAACCATCACCTTGGATT
AAAATTTTCCAAAATGNTAAATCATTTTGAGATTCTTTAAATTAATTTTTTTAAAAAAAA
AACAAAAACAAACAAAAAAATAAATAAATAAATAAAAAATTTAAATAAATAAAATT