>CHS295 (CHS295Q) /CSM/CH/CHS2-D/CHS295Q.Seq.d/
AATAAACAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACA
AAAACAAAGACAAAAACAAAAACAAAAACAAAGACAAAAACANAAACAAAAACAAAAAAC
AACAAAAAAAAAAXXXXXXXXXXGGAATNAACAAACAAAAACAAAAACAAAAACAAAAAC
AAAAACAAAAACAAAAACAAAAACAAAAACAAAGNCAAAAACAAAAACAAAAACAAAGNC
AAAAACAAAAACAAAAACAAAAAACAACAAAAAAAAAAAAAATTCAATATTTTTATTTAT
AATTACTATTATATTGTTTAACCTGTAAAGAAATCCACCACTACCACCACCACCAAAATA
TTCACACCCATTCGATCATACCAACTTCTCTTTAATCACTATAAAATAACCA

NKQTKTKTKTKTKTKTKTKTKTKTKTKTKTKTKTXTKTKNNKKK---

---NXQTKTKTKTKTKTKTKTKTKTKXKTKTKTKXKTKTKTKNNKKKKNSIFLFIITIIL
FNL*rnpplppppkyshpfdhtnfslitik*p

Frame A:
NKQTKTKTKTKTKTKTKTKTKTKTKTKTKTKTKTXTKTKNNKKK---

---gxnkqkqkqkqkqkqkqkqkqkqxqkqkqkqxqkqkqkqkttkkkkiqyfyl*llly
cltckeihhyhhhqnihthsiiptsl*sl*nn

Frame B:
inkqkqkqkqkqkqkqkqkqkqrqkqkqkqrqkxkqkqkttkkk---

---extnknknknknknknknknknkxknknknkxknknknkkqqkkkkfnifiynyyyi
v*pvkksttttttkiftpirsyqllfnhykit

Frame C:
*tnknknknknknknknknknkdknknknkdknxnknkkqqkk---

---NXQTKTKTKTKTKTKTKTKTKTKXKTKTKTKXKTKTKTKNNKKKKNSIFLFIITIIL
FNL*rnpplppppkyshpfdhtnfslitik*p

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS295 (CHS295Q) /CSM/CH/CHS2-D/CHS295Q.Seq.d/                        258   6e-68
VHL889 (VHL889Q) /CSM/VH/VHL8-D/VHL889Q.Seq.d/                         46   4e-04
SHG324 (SHG324Q) /CSM/SH/SHG3-A/SHG324Q.Seq.d/                         46   4e-04
SHD703 (SHD703Q) /CSM/SH/SHD7-A/SHD703Q.Seq.d/                         46   4e-04
SFJ578 (SFJ578Q) /CSM/SF/SFJ5-D/SFJ578Q.Seq.d/                         46   4e-04
CHH225 (CHH225Q) /CSM/CH/CHH2-B/CHH225Q.Seq.d/                         46   4e-04
AHH768 (AHH768Q) /CSM/AH/AHH7-C/AHH768Q.Seq.d/                         46   4e-04
AHF866 (AHF866Q) /CSM/AH/AHF8-C/AHF866Q.Seq.d/                         46   4e-04
AHD264 (AHD264Q) /CSM/AH/AHD2-C/AHD264Q.Seq.d/                         46   4e-04
AHB167 (AHB167Q) /CSM/AH/AHB1-C/AHB167Q.Seq.d/                         46   4e-04

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC151669|AC151669.5 Mus musculus chromosome 5, clone RP23-44L13, complete sequence.    58   1e-04  1
AC133204|AC133204.3 Mus musculus BAC clone RP23-106N3 from chromosome 5, complete sequence.    58   1e-04  1
AC119591|AC119591.5 Rattus norvegicus clone CH230-169E2, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    56   6e-04  1
AC097704|AC097704.6 Rattus norvegicus clone CH230-35E8, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    56   6e-04  1
AC102575|AC102575.11 Mus musculus chromosome 14, clone RP23-239P22, complete sequence.    54   0.002  1
BX936294|BX936294.3 Mouse DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP23-300D19.    52   0.009  1
AC132250|AC132250.1 Mus musculus chromosome UNK clone RP24-443D23, WORKING DRAFT SEQUENCE, 7 unordered pieces.    52   0.009  1
DU624241|DU624241.1 OO__Ba0129M18.f OO__Ba Oryza officinalis genomic clone OO__Ba0129M18 5', genomic survey sequence.    52   0.009  1
CR137720|CR137720.1 Forward strand read from insert in 5'HPRT insertion targeting and chromosome engineering clone MHPN30i13.    52   0.009  1
AC130659|AC130659.11 Mus musculus chromosome 1, clone RP23-326C19, complete sequence.    52   0.009  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 109,743,602

 psg: 0.44  gvh: -0.03  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.83  mip: 0.00
 nuc: 0.47  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 24.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for CHS295 is nuc

>CHS295F.Seq
AATAAACAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACA
AAAACAAAGACAAAAACAAAAACAAAAACAAAGACAAAAACANAAACAAAAACAAAAAAC
AACAAAAAAAAAANNNNNNNNNN

>CHS295Z.Seq
NNNNNNNNNNGGAATNAACAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACA
AAAACAAAAACAAAAACAAAGNCAAAAACAAAAACAAAAACAAAGNCAAAAACAAAAACA
AAAACAAAAAACAACAAAAAAAAAAAAAATTCAATATTTTTATTTATAATTACTATTATA
TTGTTTAACCTGTAAAGAAATCCACCACTACCACCACCACCAAAATATTCACACCCATTC
GATCATACCAACTTCTCTTTAATCACTATAAAATAACCA

>CHS295P.Seq
AATAAACAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACAAAAACA
AAAACAAAGACAAAAACAAAAACAAAAACAAAGACAAAAACANAAACAAAAACAAAAAAC
AACAAAAAAAAAA----------GGAATNAACAAACAAAAACAAAAACAAAAACAAAAAC
AAAAACAAAAACAAAAACAAAAACAAAAACAAAGNCAAAAACAAAAACAAAAACAAAGNC
AAAAACAAAAACAAAAACAAAAAACAACAAAAAAAAAAAAAATTCAATATTTTTATTTAT
AATTACTATTATATTGTTTAACCTGTAAAGAAATCCACCACTACCACCACCACCAAAATA
TTCACACCCATTCGATCATACCAACTTCTCTTTAATCACTATAAAATAACCA