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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH520
Library AF
(Link to library)
Clone ID AFH520
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFH520P
(Link to Original site)
Representative DNA sequence 
>AFH520 (AFH520Q) /CSM/AF/AFH5-A/AFH520Q.Seq.d/
TCAGCTAAATNCCCAAGTTTCANTNNCAAAGGTAAAGATTTAGTTCTCCAATACACTGTA
AAGAATGAACAAGNAAGTTGATCTGTGGTGGTGTCATACATCAAGTTATTACCATCCANA
TTAGATCAATCAGCATTXXXXXXXXXXAACCAGTCGATTGGGTTGACGTAAAGGAAATCG
ATGNCCCAGAAGATGTTAANCCAGCCGGTCATGNCGATATCCCAGCTTCAATCGTTGATC
CAGAAGCTGTCAANCCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAA
CCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGANAATCCCAAATCCAGAAT
ACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGNAC
TCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTT
CCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGANTTCTCTGANA
AGACCTTTGNTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGNCCTCGAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
s*xpkfxxqr*rfsspihcke*TXKLICGGVIHQVITIXIRSISI---

---PVDWVDVKEIDXPEDVXPAGHXDIPASIVDPEAVXPEDWNEEDDGEWEAPTIANPEY
KGEWKAKXIPNPEYKGEWVHPLIDNPEYAEDNXLYLFNDLGAIGFELWQVKSGSIFNNMI
VTDSVEEAKXFSXKTFXANQEAEKKMFDXLE
Translated Amino Acid sequence (All Frames) 
Frame A:
sakxpsfxxkgkdlvlqytvkneqxs*svvvsyikllpsxldqsa---

---nqsiglt*rksmxqkmlxqpvmxisqlqsliqklsxqrigmkkttvngkpqpspiqn
tkvngkqrxsqiqntkvngyth*litqntlktmxstslmisvlsvsnygklnlvpslti*
ssptlskklkxslxrplxptkkpkrkcltxs

Frame B:
qlnxqvsxxkvki*fsntl*rmnkxvdlwwchtssyyhpx*inqh---

---tsrlg*rkgnrxprrc*xsrsxrypsfnr*srscqxrgle*rrrr*mgspnhrqsri
qr*meskxnpksriqr*mgtpin**prir*rqxtlpl**srcyrfrimas*iwfhl*qyd
rhrlcrrs*rxl*xdlxcqprsrkenv*rpr

Frame C:
s*xpkfxxqr*rfsspihcke*TXKLICGGVIHQVITIXIRSISI---

---PVDWVDVKEIDXPEDVXPAGHXDIPASIVDPEAVXPEDWNEEDDGEWEAPTIANPEY
KGEWKAKXIPNPEYKGEWVHPLIDNPEYAEDNXLYLFNDLGAIGFELWQVKSGSIFNNMI
VTDSVEEAKXFSXKTFXANQEAEKKMFDXLE
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                        825   0.0
VFL283 (VFL283Q) /CSM/VF/VFL2-D/VFL283Q.Seq.d/                        825   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        825   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        825   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        825   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        825   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        825   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        825   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                        825   0.0
VFD449 (VFD449Q) /CSM/VF/VFD4-C/VFD449Q.Seq.d/                        825   0.0
own update 2001.11.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   825   0.0    3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    76   1e-13  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    76   7e-10  1
BI435832|BI435832.1 EST538593 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCK81 5' sequence, mRNA sequence.    70   4e-08  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    68   2e-07  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    68   2e-07  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    68   2e-07  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    68   2e-07  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    68   2e-07  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    68   2e-07  1
dna update 2004. 5.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   258   6e-68
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   160   2e-38
JH0795(JH0795;B31409;F60977) calreticulin precursor - California...   156   3e-37
(Q9ZNY3) RecName: Full=Calreticulin; Flags: Precursor;  &Y09816_...   155   6e-37
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   155   7e-37
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   154   1e-36
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   154   2e-36
X78057_1(X78057|pid:none) Z.mays CRH mRNA.                            152   6e-36
AK104328_1(AK104328|pid:none) Oryza sativa Japonica Group cDNA c...   152   6e-36
AF052978_1(AF052978|pid:none) Dirofilaria immitis calreticulin p...   152   6e-36
protein update 2009. 4.21
PSORT 

 psg: 0.89  gvh: 0.43  alm: 0.38  top: 0.50  tms: 0.00  mit: 0.33  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFH520 is cyt
5' end seq. ID AFH520F
5' end seq. 
>AFH520F.Seq
TCAGCTAAATNCCCAAGTTTCANTNNCAAAGGTAAAGATTTAGTTCTCCAATACACTGTA
AAGAATGAACAAGNAAGTTGATCTGTGGTGGTGTCATACATCAAGTTATTACCATCCANA
TTAGATCAATCAGCATT----------
Length of 5' end seq. 137
3' end seq. ID AFH520Z
3' end seq. 
>AFH520Z.Seq
----------AACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGNCCCAGAAGATGTT
AANCCAGCCGGTCATGNCGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAANCCA
GAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAA
TACAAAGGTGAATGGAAAGCAAAGANAATCCCAAATCCAGAATACAAAGGTGAATGGGTA
CACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGNACTCTACCTCTTTAATGAT
CTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATG
ATCGTCACCGACTCTGTCGAAGAAGCTAAAGANTTCTCTGANAAGACCTTTGNTGCCAAC
CAAGAAGCCGAAAAGAAAATGTTTGACGNCCTCGAA
Length of 3' end seq. 446
Connected seq. ID AFH520P
Connected seq. 
>AFH520P.Seq
TCAGCTAAATNCCCAAGTTTCANTNNCAAAGGTAAAGATTTAGTTCTCCAATACACTGTA
AAGAATGAACAAGNAAGTTGATCTGTGGTGGTGTCATACATCAAGTTATTACCATCCANA
TTAGATCAATCAGCATT----------AACCAGTCGATTGGGTTGACGTAAAGGAAATCG
ATGNCCCAGAAGATGTTAANCCAGCCGGTCATGNCGATATCCCAGCTTCAATCGTTGATC
CAGAAGCTGTCAANCCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAA
CCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGANAATCCCAAATCCAGAAT
ACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGNAC
TCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTT
CCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGANTTCTCTGANA
AGACCTTTGNTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGNCCTCGAA
Length of connected seq. 583
Full length Seq ID -
Full length Seq. -
Length of full length seq. -