>CHS251 (CHS251Q) /CSM/CH/CHS2-C/CHS251Q.Seq.d/
XXXXXXXXXXTGAGGAAATAATAAGTGANCCNCAATCNCANCCNCAACCAATAGCAATAG
CCTCATTAATTGAGGAAAAGAAAGATGAACAATTACAACCAAAGAAATNTGAATCACAAG
AGAATTTAACAACTTTATCAAATAGTGATAATATAATTGGTAGTGAAATTGATAGTACAA
ATACTGATAATTCTACATTATCAAGTAGTACAAATTCATTAACTATTGAAGGTAGTGCAA
GTTTGCCATTAGTTAGTGAATCATCAAACCAACAACAACAACAACAACAACAACAACAAC
CTTCATCATTAAAATCATCAACTGATCAATTCGTTGATGATAAATTATTGGATAGTACAA
CAACGACAACAGCAGTTGAAACATCAATTCAAAGAGTAGCATCAAAAGAATGTATTTTCT
CAGATATTCCTTTATCATCAGAACCATTAATGGTATCAACAAGTGTTAAAGATCCAACTT
TAGCTGCATCAACAACTAGAAAGGTTATAAAGAAAAAGGT

---EEIISXPQSXPQPIAIASLIEEKKDEQLQPKKXESQENLTTLSNSDNIIGSEIDSTN
TDNSTLSSSTNSLTIEGSASLPLVSESSNQQQQQQQQQQPSSLKSSTDQFVDDKLLDSTT
TTTAVETSIQRVASKECIFSDIPLSSEPLMVSTSVKDPTLAASTTRKVIKKK

Frame A:
---EEIISXPQSXPQPIAIASLIEEKKDEQLQPKKXESQENLTTLSNSDNIIGSEIDSTN
TDNSTLSSSTNSLTIEGSASLPLVSESSNQQQQQQQQQQPSSLKSSTDQFVDDKLLDSTT
TTTAVETSIQRVASKECIFSDIPLSSEPLMVSTSVKDPTLAASTTRKVIKKK


Frame B:
---rk**vxxnxxxnq*q*ph*lrkrkmnnynqrnxnhkri*qlyqivii*lvvklivqi
liilhyqvvqih*llkvvqvch*lvnhqtnnnnnnnnnnlhh*nhqlinslminywivqq
rqqqlkhqfke*hqknvfsqiflyhqnh*wyqqvlkiql*lhqqlerl*rkr


Frame C:
---gnnk*xxixxxtnsnslin*gker*tittkex*itrefnnfik***ynw**n**yky
**fyiik*ykfiny*r*ckfais**iikpttttttttttfiikiin*sir***iig*ynn
dnss*ninskssikrmyflrysfiirtinginkc*rsnfscinn*kgykekg

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO754 (VHO754Q) /CSM/VH/VHO7-C/VHO754Q.Seq.d/                        478   e-134
VHO208 (VHO208Q) /CSM/VH/VHO2-A/VHO208Q.Seq.d/                        478   e-134
VHL355 (VHL355Q) /CSM/VH/VHL3-C/VHL355Q.Seq.d/                        478   e-134
VHI149 (VHI149Q) /CSM/VH/VHI1-C/VHI149Q.Seq.d/                        478   e-134
VFJ386 (VFJ386Q) /CSM/VF/VFJ3-D/VFJ386Q.Seq.d/                        478   e-134
SHC156 (SHC156Q) /CSM/SH/SHC1-C/SHC156Q.Seq.d/                        478   e-134
CHS251 (CHS251Q) /CSM/CH/CHS2-C/CHS251Q.Seq.d/                        478   e-134
CHR387 (CHR387Q) /CSM/CH/CHR3-D/CHR387Q.Seq.d/                        478   e-134
CHQ829 (CHQ829Q) /CSM/CH/CHQ8-B/CHQ829Q.Seq.d/                        478   e-134
CHQ609 (CHQ609Q) /CSM/CH/CHQ6-A/CHQ609Q.Seq.d/                        478   e-134

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   436   0.0    3
AQ186890|AQ186890.1 HS_3113_B2_C07_T7 CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3113 Col=14 Row=F, genomic survey sequence.    44   0.055  2
DT661445|DT661445.1 est_e_glacialis75 RWS Eubalaena glacialis cDNA, mRNA sequence.    46   0.71   1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    46   0.71   1
AL392187|AL392187.3 Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP13-463D8.    40   1.0    2
AC139462|AC139462.1 Homo sapiens chromosome 5 clone RP11-1223D8, WORKING DRAFT SEQUENCE, 3 unordered pieces.    42   2.3    3
CO220012|CO220012.1 WS0108.B21_I05 SS-R-N-A-11 Picea sitchensis cDNA clone WS0108_I05 3', mRNA sequence.    44   2.8    1
CO218365|CO218365.1 WS0102.B21_P15 SS-R-N-A-11 Picea sitchensis cDNA clone WS0102_P15 3', mRNA sequence.    44   2.8    1
BQ593302|BQ593302.1 E012797-024-027-L07-SP6 MPIZ-ADIS-024-developing root Beta vulgaris cDNA clone 024-027-L07 5-PRIME, mRNA sequence.    44   2.8    1
BG349098|BG349098.1 947029C10.y1 947 - 2 week shoot from Barkan lab Zea mays cDNA, mRNA sequence.    44   2.8    1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3101297
Number of Hits to DB: 397,687,925

 psg: 0.75  gvh: 0.40  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: vacuolar
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHS251 is nuc

>CHS251Z.Seq
NNNNNNNNNNTGAGGAAATAATAAGTGANCCNCAATCNCANCCNCAACCAATAGCAATAG
CCTCATTAATTGAGGAAAAGAAAGATGAACAATTACAACCAAAGAAATNTGAATCACAAG
AGAATTTAACAACTTTATCAAATAGTGATAATATAATTGGTAGTGAAATTGATAGTACAA
ATACTGATAATTCTACATTATCAAGTAGTACAAATTCATTAACTATTGAAGGTAGTGCAA
GTTTGCCATTAGTTAGTGAATCATCAAACCAACAACAACAACAACAACAACAACAACAAC
CTTCATCATTAAAATCATCAACTGATCAATTCGTTGATGATAAATTATTGGATAGTACAA
CAACGACAACAGCAGTTGAAACATCAATTCAAAGAGTAGCATCAAAAGAATGTATTTTCT
CAGATATTCCTTTATCATCAGAACCATTAATGGTATCAACAAGTGTTAAAGATCCAACTT
TAGCTGCATCAACAACTAGAAAGGTTATAAAGAAAAAGGT