NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS244
Library CH
(Link to library)
Clone ID CHS244
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16507-1
Original site URL
Representative seq. ID CHS244P
(Link to Original site)
Representative DNA sequence 
>CHS244 (CHS244Q) /CSM/CH/CHS2-B/CHS244Q.Seq.d/
ATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAATTATCAAT
GAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATCA
TCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAA
ACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATAT
ACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTT
GAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTT
AAGGGTTTGGTTGAAAGTGGTCAATTGGAATTXXXXXXXXXXCCATCATGGAATTTAACC
GTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACATTCAAGAT
CCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTA
AGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGT
GTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCAT
GATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCA
TTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTNTCAATATCAA
GGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACT
NTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAA
ATTGATGGTCAAGATTCACAAGATCCNCAAACTATCACTTTAGATCTTTCAACTATCTTT
TCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCA
AATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCA
TCATCAAACNAAAAAANAANAAAAAAAAAAAAAAGAATTTTCCAAAGTA
sequence update 2002.10.25
Translated Amino Acid sequence 
IKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGWLK
TVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWDEQSTTMQNIV
KGLVESGQLE---

---PSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHR
RTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQS
SNDWNXQYQGVYSPLTSASPLPNGLKIQTXQWLDNQDNTILLRIENIYQIDGQDSQDPQT
ITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSNXKXXKKKK
RIFQS
Translated Amino Acid sequence (All Frames) 
Frame A:
IKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGWLK
TVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWDEQSTTMQNIV
KGLVESGQLE---

---PSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHR
RTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQS
SNDWNXQYQGVYSPLTSASPLPNGLKIQTXQWLDNQDNTILLRIENIYQIDGQDSQDPQT
ITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSNXKXXKKKK
RIFQS

Frame B:
*kw**knyly*ffvcf*lsmklmekkqk*milknqnqnyhqhy*mfilllilmmmlvg*k
qlmniimdqicqlhlqvfnilwiqplpvywqiqkeslfmlklhfskdggmnkvqqckill
rvwlkvvnwn---

---hhgi*pwfnqpvvimyl*mllxtfkilinhynsplslievvdvhr*emvnwi**civ
vl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnl
qtigixnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkixkl
sl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqtkkxxkkkk
efskv

Frame C:
kngnkkiiyinflfvfnyq*n*wkknknk*y*kiktktiiniikcsyccsys**cwlvkn
s**illwikyvncicrcsiyfgyshylfigksrkkvylc*ncifpkmvg*tkynnakyc*
gfg*kwsigi---

---imefnrgstnqw*lctcecycxhsrs*siitihhch**kswmcivkrwsigyddass
yfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstif
krlexsisrclltidfsltftkwfkdsnxtmvr*sr*yhtfty*kylsn*wsrftrsxny
hfrsfnylfnnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiikxkxxkkkkk
nfpk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS244 (CHS244Q) /CSM/CH/CHS2-B/CHS244Q.Seq.d/                       1850   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1334   0.0
VHP767 (VHP767Q) /CSM/VH/VHP7-C/VHP767Q.Seq.d/                       1334   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1334   0.0
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                       1334   0.0
VHO880 (VHO880Q) /CSM/VH/VHO8-D/VHO880Q.Seq.d/                       1334   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1334   0.0
VHM790 (VHM790Q) /CSM/VH/VHM7-D/VHM790Q.Seq.d/                       1334   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1334   0.0
VHI312 (VHI312Q) /CSM/VH/VHI3-A/VHI312Q.Seq.d/                       1334   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1302   0.0    1
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   8e-23  2
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   2e-13  2
CV688215|CV688215.1 sjs2-16-2_G01_sjs2-16-2G01-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   2e-13  2
CV682854|CV682854.1 service30_G09_sjs2-14-10C09-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   2e-13  2
BU798369|BU798369.1 SJF2ADB01 SJF Schistosoma japonicum cDNA, mRNA sequence.    74   2e-13  2
BU804021|BU804021.1 SJFBFG06 SJF Schistosoma japonicum cDNA, mRNA sequence.    74   2e-13  2
BU798139|BU798139.1 SJF2AAF09 SJF Schistosoma japonicum cDNA, mRNA sequence.    74   9e-13  2
BW354577|BW354577.1 Ciona intestinalis cDNA, clone:cima804e24, 5'end, single read.    66   2e-10  2
CX056208|CX056208.1 tai96f06.y2 Hydra EST UCI 5 ALP Hydra magnipapillata cDNA 5' similar to SW:MA2B_BOVIN Q29451 LYSOSOMAL ALPHA-MANNOSIDASE PRECURSOR ;, mRNA sequence.    52   3e-10  2
dna update 2005.11.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   387   e-106
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   384   e-105
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   122   2e-26
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   122   2e-26
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   121   4e-26
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   121   4e-26
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   121   4e-26
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   121   5e-26
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   121   5e-26
AY036155_1(AY036155|pid:none) Cavia porcellus lysosomal alpha-ma...   120   8e-26
protein update 2009. 4.13
PSORT 

 psg: 1.01  gvh: 0.62  alm: 0.17  top: 0.47  tms: 0.07  mit: 0.35  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for CHS244 is end
5' end seq. ID CHS244F
5' end seq. 
>CHS244F.Seq
ATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAATTATCAAT
GAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATCA
TCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAA
ACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATAT
ACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTT
GAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTT
AAGGGTTTGGTTGAAAGTGGTCAATTGGAATTNNNNNNNNNN
Length of 5' end seq. 402
3' end seq. ID CHS244Z
3' end seq. 
>CHS244Z.Seq
NNNNNNNNNNCCATCATGGAATTTAACCGTGGTTCAACCAACCAGTGGTAATTATGTACC
TGTGAATGCTATTGCNTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCAC
TGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCG
TCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAAT
CGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCG
TCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATC
TTCAAACGATTGGAATTNTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACC
TTTACCAAATGGTTTAAAGATTCAAACTNTACAATGGTTAGATAATCAAGATAATACCAT
ACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCNCAAAC
TATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAAT
GAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGA
TGGTAAAAACTATGATCATAAATCATCATCATCAAACNAAAAAANAANAAAAAAAAAAAA
AAGAATTTTCCAAAGTA
Length of 3' end seq. 737
Connected seq. ID CHS244P
Connected seq. 
>CHS244P.Seq
ATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAATTATCAAT
GAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATCA
TCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAA
ACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATAT
ACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTT
GAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTT
AAGGGTTTGGTTGAAAGTGGTCAATTGGAATT----------CCATCATGGAATTTAACC
GTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACATTCAAGAT
CCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTA
AGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGT
GTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCAT
GATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCA
TTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTNTCAATATCAA
GGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACT
NTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAA
ATTGATGGTCAAGATTCACAAGATCCNCAAACTATCACTTTAGATCTTTCAACTATCTTT
TCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCA
AATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCA
TCATCAAACNAAAAAANAANAAAAAAAAAAAAAAGAATTTTCCAAAGTA
Length of connected seq. 1119
Full length Seq ID -
Full length Seq. -
Length of full length seq. -