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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS185
Library CH
(Link to library)
Clone ID CHS185
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10823-1
Original site URL
Representative seq. ID CHS185P
(Link to Original site)
Representative DNA sequence 
>CHS185 (CHS185Q) /CSM/CH/CHS1-D/CHS185Q.Seq.d/
AAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATTATTTTTTT
TGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAATATCCAGG
TTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTTAACCTTAT
TTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACTTTTGAAAC
TAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAAGTCCCACC
AACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATTGAATTTAC
AAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGTGAAGTTTTATTTAATAC
AACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTATTTANAATT
AAGTACATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGGAGXXXXXXXXXXTGG
TACAGGTTTAATGGTATCACCAGTTCTCACTCAAGGTGCTACCACAAGTGAATGCTTACT
TCCCAAATGATATCTGGTATGAATATGGTAATGGTTCATTGGTTCAATCAGTTGGTACCC
ATCAAACTTTAAATGCTCCATTCGATGTAATCAACGTTCATATGCGTGGTGGTAATATCA
TTCCAACTCAACCAACCTCCTCATATGTTACACCAGTTGATGGTATTCCAATTACCACTA
AAATCTCTAGAACTTTACCATTTGAATTGATTATTGCCTTGGATTCTTCATTACAAGCAA
CTGGTCAATTATTCTTGGATGATGGTGAATCAATTCAAACCTATGTTGATAATAAATACT
CTTTCATTCAATTCGATGTTGTCTCCTCACCATCTTCATCTGCCTACAAATTACAATCAA
CCATTCTCAATAACAATTATAATGGTACCGCTTCTTTAATCATTAATTCTATCCAAATCT
ACGGTTCGCCATCAGTTCAACAAGTCATTGTTAATGGTAGCCCAATCAATTCATTTAATG
CTGTTTCTGATTCAACTCTCTCTGTTTCAAATTTACAACTTGCTTTAGATGAATCCTTTG
AAGTNGATTTTGTATTATATTAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
ki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATLNLI
SAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYIIEFT
NNPFGFAATRISTGEVLFNTTQPSDCSFNGLIYSNYYLXLSTSFTESNPNIYGLG---

---GTGLMVSPVLTQGATTSECLLPK*ylv*iw*wfigsiswypsnfkcsircnqrsyaw
w*yhsnstnllicyts*wysnyh*nl*nfti*idyclgffitsnwsiilg*w*insnlc*
**ilfhsircclltificlqitinhsq*ql*wyrffnh*fypnlrfaisstshc*w*pnq
fi*ccf*fnslcfkfttcfr*il*sxfciilk
Translated Amino Acid sequence (All Frames) 
Frame A:
knin*n*kknetfvfinyffcyciilxcwiiisiim*isrlfnsrxyknk*wl*gnt*py
fsrsiw*ryxtikfsinf*n*snfxsxny*pk*skmgsptnc*siswxksxfn*lyn*iy
k*siwfcsnsyfnw*sfi*ynstk*lfi*wfnlfkllfxikyiihre*skylwfr---

---wyrfngitsshsrcyhk*mltsqmisgmnmvmvhwfnqlvpikl*mlhsm*stficv
vvisfqlnqpphmlhqlmvfqlplkslelyhln*llpwilhykqlvnyswmmvnqfkpml
iintlsfnsmlsphhlhlptnynqpfsitiimvpll*slilskstvrhqfnksllmvaqs
ihlmlfliqlslfqiynll*mnplkxilyyik

Frame B:
ki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATLNLI
SAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYIIEFT
NNPFGFAATRISTGEVLFNTTQPSDCSFNGLIYSNYYLXLSTSFTESNPNIYGLG---

---GTGLMVSPVLTQGATTSECLLPK*ylv*iw*wfigsiswypsnfkcsircnqrsyaw
w*yhsnstnllicyts*wysnyh*nl*nfti*idyclgffitsnwsiilg*w*insnlc*
**ilfhsircclltificlqitinhsq*ql*wyrffnh*fypnlrfaisstshc*w*pnq
fi*ccf*fnslcfkfttcfr*il*sxfciilk

Frame C:
kyklklkkk*nfclh*lfflllyyfxlldhyqhhyvniqviqlkxlqkqimvmrqhltlf
qqvhmvtixnn*ifn*llklvkflxlxlltqiikdgkshqllin*lxkixiqlii*lnlq
iihlvlqqlvfqlvkfyliqlnqvivhlmv*fiqiiixn*vhhsqrviqifmv*e---

---vqv*wyhqfslkvlpqvnayfpndiwyeygngslvqsvgthqtlnapfdvinvhmrg
gniiptqptssyvtpvdgipittkisrtlpfeliialdsslqatgqlflddgesiqtyvd
nkysfiqfdvvsspsssayklqstilnnnyngtasliinsiqiygspsvqqvivngspin
sfnavsdstlsvsnlqlaldesfevdfvly*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS185 (CHS185Q) /CSM/CH/CHS1-D/CHS185Q.Seq.d/                       2093   0.0
SHC731 (SHC731Q) /CSM/SH/SHC7-B/SHC731Q.Seq.d/                       1225   0.0
CHR858 (CHR858Q) /CSM/CH/CHR8-C/CHR858Q.Seq.d/                       1225   0.0
CHM276 (CHM276Q) /CSM/CH/CHM2-D/CHM276Q.Seq.d/                       1225   0.0
CHH383 (CHH383Q) /CSM/CH/CHH3-D/CHH383Q.Seq.d/                       1225   0.0
AHR217 (AHR217Q) /CSM/AH/AHR2-A/AHR217Q.Seq.d/                       1225   0.0
AHN674 (AHN674Q) /CSM/AH/AHN6-D/AHN674Q.Seq.d/                       1225   0.0
AHM502 (AHM502Q) /CSM/AH/AHM5-A/AHM502Q.Seq.d/                       1225   0.0
AHF873 (AHF873Q) /CSM/AH/AHF8-D/AHF873Q.Seq.d/                       1225   0.0
AHD417 (AHD417Q) /CSM/AH/AHD4-A/AHD417Q.Seq.d/                       1225   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    32   0.46   9
Z70754|Z70754.2 Caenorhabditis elegans Cosmid F58E6.               42   0.47   4
AC116032|AC116032.2 Dictyostelium discoideum chromosome 2 map 4158743-4189373 strain AX4, complete sequence.    36   0.48   5
AL449924|AL449924.1 Streptococcus pneumoniae serotype 19F  *** SEQUENCING IN PROGRESS  *** from clone G54.    36   0.79   5
AE014822|AE014822.1 Plasmodium falciparum 3D7 chromosome 14 section 7 of 13 of the complete sequence.    32   3.0    2
AZ690043|AZ690043.1 ENTMH81TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36   3.5    3
Z83228|Z83228.1 Caenorhabditis elegans Cosmid F52F12.              34   3.6    4
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    40   5.4    8
AR544895|AR544895.1 Sequence 26 from patent US 6747137.            40   6.0    2
DR573447|DR573447.1 WS00734.B21_O19 WS-PS-N-A-8 Picea glauca cDNA clone WS00734_O19 3, mRNA sequence.    44   6.4    1
dna update 2005.11.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU937530_1(EU937530|pid:none) Mus musculus sucrase-isomaltase mR...   101   5e-20
AB000967_1(AB000967|pid:none) Coturnix japonica GAAI mRNA for ac...   100   1e-19
AF016833_1(AF016833|pid:none) Homo sapiens maltase-glucoamylase ...    95   7e-19
AF118226_1(AF118226|pid:none) Hordeum vulgare high pI alpha-gluc...    90   7e-19
L25926_1(L25926|pid:none) Rat sucrase-isomaltase (SI) mRNA, comp...    97   1e-18
(P23739) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    97   1e-18
(O43451) RecName: Full=Maltase-glucoamylase, intestinal; Include...    93   2e-18
BC115034_1(BC115034|pid:none) Homo sapiens sucrase-isomaltase (a...    94   7e-18
(P14410) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    94   7e-18
BC116452_1(BC116452|pid:none) Homo sapiens sucrase-isomaltase (a...    94   9e-18
protein update 2009. 4.13
PSORT 

 psg: 1.02  gvh: 0.72  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for CHS185 is exc
5' end seq. ID CHS185F
5' end seq. 
>CHS185F.Seq
AAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATTATTTTTTT
TGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAATATCCAGG
TTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTTAACCTTAT
TTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACTTTTGAAAC
TAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAAGTCCCACC
AACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATTGAATTTAC
AAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGTGAAGTTTTATTTAATAC
AACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTATTTANAATT
AAGTACATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGGAGNNNNNNNNNN
Length of 5' end seq. 537
3' end seq. ID CHS185Z
3' end seq. 
>CHS185Z.Seq
NNNNNNNNNNTGGTACAGGTTTAATGGTATCACCAGTTCTCACTCAAGGTGCTACCACAA
GTGAATGCTTACTTCCCAAATGATATCTGGTATGAATATGGTAATGGTTCATTGGTTCAA
TCAGTTGGTACCCATCAAACTTTAAATGCTCCATTCGATGTAATCAACGTTCATATGCGT
GGTGGTAATATCATTCCAACTCAACCAACCTCCTCATATGTTACACCAGTTGATGGTATT
CCAATTACCACTAAAATCTCTAGAACTTTACCATTTGAATTGATTATTGCCTTGGATTCT
TCATTACAAGCAACTGGTCAATTATTCTTGGATGATGGTGAATCAATTCAAACCTATGTT
GATAATAAATACTCTTTCATTCAATTCGATGTTGTCTCCTCACCATCTTCATCTGCCTAC
AAATTACAATCAACCATTCTCAATAACAATTATAATGGTACCGCTTCTTTAATCATTAAT
TCTATCCAAATCTACGGTTCGCCATCAGTTCAACAAGTCATTGTTAATGGTAGCCCAATC
AATTCATTTAATGCTGTTTCTGATTCAACTCTCTCTGTTTCAAATTTACAACTTGCTTTA
GATGAATCCTTTGAAGTNGATTTTGTATTATATTAAAAA
Length of 3' end seq. 639
Connected seq. ID CHS185P
Connected seq. 
>CHS185P.Seq
AAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATTATTTTTTT
TGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAATATCCAGG
TTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTTAACCTTAT
TTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACTTTTGAAAC
TAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAAGTCCCACC
AACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATTGAATTTAC
AAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGTGAAGTTTTATTTAATAC
AACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTATTTANAATT
AAGTACATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGGAG----------TGG
TACAGGTTTAATGGTATCACCAGTTCTCACTCAAGGTGCTACCACAAGTGAATGCTTACT
TCCCAAATGATATCTGGTATGAATATGGTAATGGTTCATTGGTTCAATCAGTTGGTACCC
ATCAAACTTTAAATGCTCCATTCGATGTAATCAACGTTCATATGCGTGGTGGTAATATCA
TTCCAACTCAACCAACCTCCTCATATGTTACACCAGTTGATGGTATTCCAATTACCACTA
AAATCTCTAGAACTTTACCATTTGAATTGATTATTGCCTTGGATTCTTCATTACAAGCAA
CTGGTCAATTATTCTTGGATGATGGTGAATCAATTCAAACCTATGTTGATAATAAATACT
CTTTCATTCAATTCGATGTTGTCTCCTCACCATCTTCATCTGCCTACAAATTACAATCAA
CCATTCTCAATAACAATTATAATGGTACCGCTTCTTTAATCATTAATTCTATCCAAATCT
ACGGTTCGCCATCAGTTCAACAAGTCATTGTTAATGGTAGCCCAATCAATTCATTTAATG
CTGTTTCTGATTCAACTCTCTCTGTTTCAAATTTACAACTTGCTTTAGATGAATCCTTTG
AAGTNGATTTTGTATTATATTAAAAA
Length of connected seq. 1156
Full length Seq ID -
Full length Seq. -
Length of full length seq. -