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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS182
Library CH
(Link to library)
Clone ID CHS182
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10924-1|Contig-U12653-1
Original site URL
Representative seq. ID CHS182P
(Link to Original site)
Representative DNA sequence 
>CHS182 (CHS182Q) /CSM/CH/CHS1-D/CHS182Q.Seq.d/
ATTAAAAATTAATATAAATAAAATATAAAATAAAATGTCTACAGCAAAATTAGATTTTGT
TAGAGCATATGAAAAAGAAGTTCAAAATAAATGGGAAAGTGATAAACAATTTGAAATTGA
TGCATTAGATGCACCAGATGCTGAACATCCAAAATATTTAGCAACATTCCCATACCCATA
TATGAATGGAAGATTACATGTTGGTCATGTTTTCACAATCACCAAAGCAGAATTTATGTG
TCAATTCCAAAGATTAATGGGTAAAAGAGTTTTATTCCCATTTGCATTCCATTGTACTGG
TATGCCAATTAAAGCATGTGCTGATAAATTAAAGAAAGAAATTGAACAATTTGGTTGTCC
ACCAGTTTTCCCAGTTATTGAAGAAAAACCAATTCAAGAAGTAACAACAGCAGTAAAAGA
AGATCCACTTTCATTTAAATCAAAGAAAACAAAAGCAGTAGCAAAATCAGGAGGAGCAGT
ATATCAATGGAAGATTATGCAAAGTTTAGGTATTTCAGATGAAGAGATTCCAATGTTTGC
AGATTCAGCCTATTGGTTAAATTATTTCCCACCACATTGTGAAAGTGATCTTAAATTATT
XXXXXXXXXXCAATATCATTGTAGAATCTGAAAAAGCTTATCAAAAANCCAATTTCNGTG
ATGCTCTTCATTTAGTATTCTTTGATCTCCAAAATGCTCGTGATCACTACAAAGTAACCA
CNTTAGATCAAATGCACAAGGATCTCGTTTTAAGATTCATTGAAGTTCAAGCTTTATTAA
TTTATCCAATCGCTCCACATTTTGCTCAAAAACTTTTCAATATGCTTGGAAAAGGTAATA
TCTTAAGTGCCGGTTGGCCAAAAGCTGGTGCAATCGATTATGAAGCTTTGAAAAAGAATA
ACTATGTTCAACAAACCATTTACAATTTCAGAATGAAATTACAAGTTTATCAAAAGGCAA
AATTAAAAGGTAAACCAGCTGGTACTAAAGCAATTCCAGATCAATCAACCATCATTGTAA
GTAAGAGTTATCCAAAATGGCAACAAGATGTCTTGGAATACCTTGCCACCATCTACAATG
AAGATTCAAAATCTTTCACAAAAGATAACAATGCCATCGCTGAAGAATTATTATCTCGTG
AAGAAATGAAACCACATAAAAAAAATCTTATGGGTTTTGTCGCTTCTGCAATTCAAAATG
TTAAAGAATCTGGTAAAGATGCTTTACAAACTTCTCTCTCATTTGATGAAACTTCAACTC
TCACTGATAATATTGATTATATTTGTAAAACTTTAGAATTAACTAGTTTCGATGTTAAAG
ATTTCAATGAAATCCCNCAAGCTTCTCAATCAAAAANACAACCAATTACCAGGTAAACCA
CAATTCCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
lkininki*NKMSTAKLDFVRAYEKEVQNKWESDKQFEIDALDAPDAEHPKYLATFPYPY
MNGRLHVGHVFTITKAEFMCQFQRLMGKRVLFPFAFHCTGMPIKACADKLKKEIEQFGCP
PVFPVIEEKPIQEVTTAVKEDPLSFKSKKTKAVAKSGGAVYQWKIMQSLGISDEEIPMFA
DSAYWLNYFPPHCESDLKL---

---NIIVESEKAYQKXNFXDALHLVFFDLQNARDHYKVTTLDQMHKDLVLRFIEVQALLI
YPIAPHFAQKLFNMLGKGNILSAGWPKAGAIDYEALKKNNYVQQTIYNFRMKLQVYQKAK
LKGKPAGTKAIPDQSTIIVSKSYPKWQQDVLEYLATIYNEDSKSFTKDNNAIAEELLSRE
EMKPHKKNLMGFVASAIQNVKESGKDALQTSLSFDETSTLTDNIDYICKTLELTSFDVKD
FNEIPQASQSKXQPITR*ttip
Translated Amino Acid sequence (All Frames) 
Frame A:
ikn*yk*nik*nvyskirfc*si*krssk*mgk**ti*n*circtrc*tskifsnipipi
yewkitcwscfhnhqsriyvsipking*ksfipiciplywyan*smc**ikern*tiwls
tsfpsy*rktnsrsnnsskrrstfi*ikenkssskirrssismedyakfryfr*rdsnvc
rfsllvklfpttl*k*s*ii---

---qyhcri*kslskxqfx*cssfsil*spkcs*slqsnhxrsnaqgsrfkih*sssfin
lsnrstfcsktfqyawkr*ylkcrlakswcnrl*sfeke*lcstnhlqfqneitslskgk
ikr*tswy*snsrsinhhck*elskmatrclgipchhlq*rfkifhkr*qchr*riiis*
rnett*kksygfcrfcnskc*riw*rcftnfsli**nfnsh**y*lyl*nfrin*frc*r
fq*npxsfsikxttnyqvnhns

Frame B:
lkininki*NKMSTAKLDFVRAYEKEVQNKWESDKQFEIDALDAPDAEHPKYLATFPYPY
MNGRLHVGHVFTITKAEFMCQFQRLMGKRVLFPFAFHCTGMPIKACADKLKKEIEQFGCP
PVFPVIEEKPIQEVTTAVKEDPLSFKSKKTKAVAKSGGAVYQWKIMQSLGISDEEIPMFA
DSAYWLNYFPPHCESDLKL---

---NIIVESEKAYQKXNFXDALHLVFFDLQNARDHYKVTTLDQMHKDLVLRFIEVQALLI
YPIAPHFAQKLFNMLGKGNILSAGWPKAGAIDYEALKKNNYVQQTIYNFRMKLQVYQKAK
LKGKPAGTKAIPDQSTIIVSKSYPKWQQDVLEYLATIYNEDSKSFTKDNNAIAEELLSRE
EMKPHKKNLMGFVASAIQNVKESGKDALQTSLSFDETSTLTDNIDYICKTLELTSFDVKD
FNEIPQASQSKXQPITR*ttip

Frame C:
*kli*ikykikclqqn*illehmkkkfkingkvinnlklmh*mhqmlniqni*qhshthi
*medymlvmfsqspkqnlcvnskd*wvkefyshlhsivlvcqlkhvlin*rkklnnlvvh
qfsqllkknqfkk*qqq*kkihfhlnqrkqkq*qnqeeqyingrlckv*vfqmkrfqclq
iqpig*iishhivkvilny---

---isl*nlkklikxpisvmlfi*ysliskmlvittk*px*ikctrisf*dslkfkly*f
iqslhillknfsiclekvis*vpvgqklvqsimkl*kritmfnkpftise*nykfikrqn
*kvnqlvlkqfqinqpsl*vrviqngnkmswntlppstmkiqnlsqkitmpslknyylvk
k*nhikkilwvlsllqfkmlknlvkmlykllshlmklqlsliiliifvkl*n*lvsmlki
smksxkllnqkxnqlpgkpqfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS182 (CHS182Q) /CSM/CH/CHS1-D/CHS182Q.Seq.d/                       2545   0.0
AHQ494 (AHQ494Q) /CSM/AH/AHQ4-D/AHQ494Q.Seq.d/                       1449   0.0
AHD312 (AHD312Q) /CSM/AH/AHD3-A/AHD312Q.Seq.d/                       1449   0.0
CHM435 (CHM435Q) /CSM/CH/CHM4-B/CHM435Q.Seq.d/                       1447   0.0
AHC831 (AHC831Q) /CSM/AH/AHC8-B/AHC831Q.Seq.d/                       1443   0.0
VHP654 (VHP654Q) /CSM/VH/VHP6-C/VHP654Q.Seq.d/                       1407   0.0
SHG191 (SHG191Q) /CSM/SH/SHG1-D/SHG191Q.Seq.d/                       1394   0.0
CHE666 (CHE666Q) /CSM/CH/CHE6-C/CHE666Q.Seq.d/                       1392   0.0
SHC504 (SHC504Q) /CSM/SH/SHC5-A/SHC504Q.Seq.d/                       1358   0.0
AHI241 (AHI241Q) /CSM/AH/AHI2-B/AHI241Q.Seq.d/                       1330   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ539678|AZ539678.1 ENTCV87TR Entamoeba histolytica Sheared DNA Entamoeba histolyticagenomic, genomic survey sequence.    94   4e-20  2
BH134624|BH134624.1 ENTNX81TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    94   4e-20  2
AZ692319|AZ692319.1 ENTII93TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    94   4e-20  2
CB389922|CB389922.1 OSTF126G7_1 AD-wrmcDNA Caenorhabditis elegans cDNA, mRNA sequence.    66   7e-11  2
AZ686396|AZ686396.1 ENTKF49TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    78   1e-10  2
CR382138|CR382138.1 Debaryomyces hansenii chromosome F of strain CBS767 of Debaryomyces hansenii.    76   2e-09  1
Z36238|Z36238.1 Caenorhabditis elegans Cosmid R74.                 66   5e-09  3
AX240824|AX240824.1 Sequence 62 from Patent WO0160975.             58   2e-08  3
AR578978|AR578978.1 Sequence 62 from patent US 6783985.            58   2e-08  3
AX488736|AX488736.1 Sequence 6036 from Patent WO02053728.          58   2e-08  3
dna update 2005.11.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC079713_1(BC079713|pid:none) Xenopus laevis MGC82093 protein, m...   218   3e-55
BC090117_1(BC090117|pid:none) Xenopus tropicalis MGC97760 protei...   218   5e-55
BC087655_1(BC087655|pid:none) Rattus norvegicus leucyl-tRNA synt...   216   2e-54
(Q9P2J5) RecName: Full=Leucyl-tRNA synthetase, cytoplasmic;     ...   215   3e-54
D84223_1(D84223|pid:none) Homo sapiens mRNA for leucyl tRNA synt...   215   3e-54
AK315178_1(AK315178|pid:none) Homo sapiens cDNA, FLJ96156, highl...   215   3e-54
BC068041_1(BC068041|pid:none) Homo sapiens leucyl-tRNA synthetas...   215   3e-54
(Q09996) RecName: Full=Leucyl-tRNA synthetase;          EC=6.1.1...   214   4e-54
AE014134_647(AE014134|pid:none) Drosophila melanogaster chromoso...   213   1e-53
AK030778_1(AK030778|pid:none) Mus musculus 8 days embryo whole b...   212   3e-53
protein update 2009. 4.13
PSORT 

 psg: 0.44  gvh: 0.30  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for CHS182 is nuc
5' end seq. ID CHS182F
5' end seq. 
>CHS182F.Seq
ATTAAAAATTAATATAAATAAAATATAAAATAAAATGTCTACAGCAAAATTAGATTTTGT
TAGAGCATATGAAAAAGAAGTTCAAAATAAATGGGAAAGTGATAAACAATTTGAAATTGA
TGCATTAGATGCACCAGATGCTGAACATCCAAAATATTTAGCAACATTCCCATACCCATA
TATGAATGGAAGATTACATGTTGGTCATGTTTTCACAATCACCAAAGCAGAATTTATGTG
TCAATTCCAAAGATTAATGGGTAAAAGAGTTTTATTCCCATTTGCATTCCATTGTACTGG
TATGCCAATTAAAGCATGTGCTGATAAATTAAAGAAAGAAATTGAACAATTTGGTTGTCC
ACCAGTTTTCCCAGTTATTGAAGAAAAACCAATTCAAGAAGTAACAACAGCAGTAAAAGA
AGATCCACTTTCATTTAAATCAAAGAAAACAAAAGCAGTAGCAAAATCAGGAGGAGCAGT
ATATCAATGGAAGATTATGCAAAGTTTAGGTATTTCAGATGAAGAGATTCCAATGTTTGC
AGATTCAGCCTATTGGTTAAATTATTTCCCACCACATTGTGAAAGTGATCTTAAATTATT
NNNNNNNNNN
Length of 5' end seq. 610
3' end seq. ID CHS182Z
3' end seq. 
>CHS182Z.Seq
NNNNNNNNNNCAATATCATTGTAGAATCTGAAAAAGCTTATCAAAAANCCAATTTCNGTG
ATGCTCTTCATTTAGTATTCTTTGATCTCCAAAATGCTCGTGATCACTACAAAGTAACCA
CNTTAGATCAAATGCACAAGGATCTCGTTTTAAGATTCATTGAAGTTCAAGCTTTATTAA
TTTATCCAATCGCTCCACATTTTGCTCAAAAACTTTTCAATATGCTTGGAAAAGGTAATA
TCTTAAGTGCCGGTTGGCCAAAAGCTGGTGCAATCGATTATGAAGCTTTGAAAAAGAATA
ACTATGTTCAACAAACCATTTACAATTTCAGAATGAAATTACAAGTTTATCAAAAGGCAA
AATTAAAAGGTAAACCAGCTGGTACTAAAGCAATTCCAGATCAATCAACCATCATTGTAA
GTAAGAGTTATCCAAAATGGCAACAAGATGTCTTGGAATACCTTGCCACCATCTACAATG
AAGATTCAAAATCTTTCACAAAAGATAACAATGCCATCGCTGAAGAATTATTATCTCGTG
AAGAAATGAAACCACATAAAAAAAATCTTATGGGTTTTGTCGCTTCTGCAATTCAAAATG
TTAAAGAATCTGGTAAAGATGCTTTACAAACTTCTCTCTCATTTGATGAAACTTCAACTC
TCACTGATAATATTGATTATATTTGTAAAACTTTAGAATTAACTAGTTTCGATGTTAAAG
ATTTCAATGAAATCCCNCAAGCTTCTCAATCAAAAANACAACCAATTACCAGGTAAACCA
CAATTCCAA
Length of 3' end seq. 789
Connected seq. ID CHS182P
Connected seq. 
>CHS182P.Seq
ATTAAAAATTAATATAAATAAAATATAAAATAAAATGTCTACAGCAAAATTAGATTTTGT
TAGAGCATATGAAAAAGAAGTTCAAAATAAATGGGAAAGTGATAAACAATTTGAAATTGA
TGCATTAGATGCACCAGATGCTGAACATCCAAAATATTTAGCAACATTCCCATACCCATA
TATGAATGGAAGATTACATGTTGGTCATGTTTTCACAATCACCAAAGCAGAATTTATGTG
TCAATTCCAAAGATTAATGGGTAAAAGAGTTTTATTCCCATTTGCATTCCATTGTACTGG
TATGCCAATTAAAGCATGTGCTGATAAATTAAAGAAAGAAATTGAACAATTTGGTTGTCC
ACCAGTTTTCCCAGTTATTGAAGAAAAACCAATTCAAGAAGTAACAACAGCAGTAAAAGA
AGATCCACTTTCATTTAAATCAAAGAAAACAAAAGCAGTAGCAAAATCAGGAGGAGCAGT
ATATCAATGGAAGATTATGCAAAGTTTAGGTATTTCAGATGAAGAGATTCCAATGTTTGC
AGATTCAGCCTATTGGTTAAATTATTTCCCACCACATTGTGAAAGTGATCTTAAATTATT
----------CAATATCATTGTAGAATCTGAAAAAGCTTATCAAAAANCCAATTTCNGTG
ATGCTCTTCATTTAGTATTCTTTGATCTCCAAAATGCTCGTGATCACTACAAAGTAACCA
CNTTAGATCAAATGCACAAGGATCTCGTTTTAAGATTCATTGAAGTTCAAGCTTTATTAA
TTTATCCAATCGCTCCACATTTTGCTCAAAAACTTTTCAATATGCTTGGAAAAGGTAATA
TCTTAAGTGCCGGTTGGCCAAAAGCTGGTGCAATCGATTATGAAGCTTTGAAAAAGAATA
ACTATGTTCAACAAACCATTTACAATTTCAGAATGAAATTACAAGTTTATCAAAAGGCAA
AATTAAAAGGTAAACCAGCTGGTACTAAAGCAATTCCAGATCAATCAACCATCATTGTAA
GTAAGAGTTATCCAAAATGGCAACAAGATGTCTTGGAATACCTTGCCACCATCTACAATG
AAGATTCAAAATCTTTCACAAAAGATAACAATGCCATCGCTGAAGAATTATTATCTCGTG
AAGAAATGAAACCACATAAAAAAAATCTTATGGGTTTTGTCGCTTCTGCAATTCAAAATG
TTAAAGAATCTGGTAAAGATGCTTTACAAACTTCTCTCTCATTTGATGAAACTTCAACTC
TCACTGATAATATTGATTATATTTGTAAAACTTTAGAATTAACTAGTTTCGATGTTAAAG
ATTTCAATGAAATCCCNCAAGCTTCTCAATCAAAAANACAACCAATTACCAGGTAAACCA
CAATTCCAA
Length of connected seq. 1379
Full length Seq ID -
Full length Seq. -
Length of full length seq. -