>CHS181 (CHS181Q) /CSM/CH/CHS1-D/CHS181Q.Seq.d/
XXXXXXXXXXCATTCATTGAAGGTCTCAAGAAGAAGAATCTTGAAGTCATTTACATGTGT
GACCCAATTGATGAATATGCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTA
TCAATTACCAAAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAA
CAAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGATAAA
GTTGAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCT
GAATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCT
TCAATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATC
GTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTAC
TTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTT
GCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCA
ACAACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAAC
GATGAACCATCTGAAAGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGCTATCAA
TTTTTTGGAATATTGTTATATTATTATAATAAAATAAATTTTAAAAAAAATCTTCA

---FIEGLKKKNLEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQ
DKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSS
MSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFA
SRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSERKKSTRFNY*pfcyqf
fgillyyynkinfkknl

Frame A:
---ih*rsqeees*shlhv*pn**iccstikri*r*eisinyqrrfeir*nrr*keks*t
r*scq*ritqts*rcig**s*kgsslnsscqltmcfsyl*iwlvskhgtyhessssp*lf
nviihvikenfriksrspnr*rfsqksrrkk*nfqrlcllii*ncslnfrflir*tiiic
ftyssyd*irfkhsr*fisnn*rinkhnnq**ystirrkr*ti*kekvd*i*llalllsi
fwnivill**nkf*kkss


Frame B:
---FIEGLKKKNLEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQ
DKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSS
MSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFA
SRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSERKKSTRFNY*pfcyqf
fgillyyynkinfkknl


Frame C:
---slkvsrrrilksftcvtqlmnmlfnn*knmtvrn*yqlpkkv*n*mkpkmkrkklnk
iklpmknysnklkmywviklkr*fsqlvlpthhvf*lplnmvgqqtwnvs*klklsvtlq
chhtchqrkl*n*iqitqslei*pkkppkkvklsktlftyymklls*lqvsh*mnhhhll
hvfiv*ln*v*afkmihqqqlknqqtqqpvmifhh*kktmnhlkgksrldlitspfainf
leycyiiiik*ilkkif

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ442 (VHQ442Q) /CSM/VH/VHQ4-B/VHQ442Q.Seq.d/                        842   0.0
VHP779 (VHP779Q) /CSM/VH/VHP7-D/VHP779Q.Seq.d/                        842   0.0
VHO658 (VHO658Q) /CSM/VH/VHO6-C/VHO658Q.Seq.d/                        842   0.0
VHN866 (VHN866Q) /CSM/VH/VHN8-C/VHN866Q.Seq.d/                        842   0.0
VHN567 (VHN567Q) /CSM/VH/VHN5-C/VHN567Q.Seq.d/                        842   0.0
VHJ833 (VHJ833Q) /CSM/VH/VHJ8-B/VHJ833Q.Seq.d/                        842   0.0
VHI507 (VHI507Q) /CSM/VH/VHI5-A/VHI507Q.Seq.d/                        842   0.0
VHI392 (VHI392Q) /CSM/VH/VHI3-D/VHI392Q.Seq.d/                        842   0.0
VHH735 (VHH735Q) /CSM/VH/VHH7-B/VHH735Q.Seq.d/                        842   0.0
VHD783 (VHD783Q) /CSM/VH/VHD7-D/VHD783Q.Seq.d/                        842   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L43591|L43591.1 Dictyostelium discoideum (clone C 6.1.1) heat shock cognate protein (hsc90) mRNA, complete cds.   842   0.0    3
BM162508|BM162508.1 EST565031 PyBS Plasmodium yoelii yoelii cDNA clone PYCKT49 5' end, mRNA sequence.    72   3e-25  4
BM168747|BM168747.1 EST571270 PyBS Plasmodium yoelii yoelii cDNA clone PYCPF64 5' end, mRNA sequence.    72   3e-25  4
AY122634|AY122634.1 Naegleria gruberi strain ATCC 30224 heat shock protein 90 (HSP-90) gene, partial cds.    82   2e-23  4
AF165818|AF165818.4 Guillardia theta nucleomorph chromosome 1, complete sequence.    80   9e-22  8
DT619331| heat shock protein 90 (AA 1 - 728) [Gallus gallus], mRNA sequence.    64   3e-20  3
DN705426|DN705426.1 CLJ59-D07.x1d-t SHGC-CLJ Gasterosteus aculeatus cDNA clone CLJ59-D07 3', mRNA sequence.    68   8e-20  2
L34028|L34028.1 Plasmodium falciparum (clone HB3) heat shock protein 86 gene, complete cds.    60   2e-19  4
L34027|L34027.1 Plasmodium falciparum (clone Dd2) heat shock protein 86 gene, complete cds.    60   1e-18  4
AF030694|AF030694.2 Plasmodium falciparum strain Dd2 heat shock protein 86 (HSP86), O1 (o1), O3 (o3), O2 (o2), CG8 (cg8), CG4 (cg4), CG3 (cg3), putative chloroquine resistance transporter (crt), CG9 (cg9), CG1 (cg1), CG6 (cg6), CG2 (cg2), and CG7 (cg7) genes, complete cds.    60   2e-18  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             315   1e-84
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   315   1e-84
AB092411_1(AB092411|pid:none) Entamoeba histolytica gene for cyt...   221   1e-56
DQ062086_1(DQ062086|pid:none) Pseudourostyla cristata heat shock...   218   2e-55
AY292370_1(AY292370|pid:none) Toxoplasma gondii HSP90 mRNA, comp...   214   2e-54
DQ884433_1(DQ884433|pid:none) Karlodinium micrum clone Kmi-5p-cD...   214   2e-54
AY831450_1(AY831450|pid:none) Monocercomonoides sp. PA203 heat s...   213   7e-54
AM426751_2(AM426751|pid:none) Vitis vinifera contig VV78X138759....   212   1e-53
DQ144978_1(DQ144978|pid:none) Symbiodinium sp. C3 heat shock pro...   211   2e-53
AF402101_1(AF402101|pid:none) Achlya ambisexualis isolate JS855 ...   211   2e-53

 psg: 0.80  gvh: 0.15  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for CHS181 is nuc

>CHS181Z.Seq
NNNNNNNNNNCATTCATTGAAGGTCTCAAGAAGAAGAATCTTGAAGTCATTTACATGTGT
GACCCAATTGATGAATATGCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTA
TCAATTACCAAAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAA
CAAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGATAAA
GTTGAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCT
GAATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCT
TCAATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATC
GTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTAC
TTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTT
GCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCA
ACAACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAAC
GATGAACCATCTGAAAGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGCTATCAA
TTTTTTGGAATATTGTTATATTATTATAATAAAATAAATTTTAAAAAAAATCTTCA