>CHS180 (CHS180Q) /CSM/CH/CHS1-D/CHS180Q.Seq.d/
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAA
ANGGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAA
AATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATT
CAAAGAGCTGGCATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGT
CCANATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAA
ATGCCAAAAGATGANAAATTACCAACTGGTTGTAGCTCACACGAATTCTTGATCAATCTT
ATTGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAGCAGCACTTCGTGTTACC
GATGGTGCATTGGTAGTTATXXXXXXXXXXTGCAAAATCACCAAATAAACACAATCGTAT
CTACATGAAATCTGAACCATTATCGTCAGACCTAACTGATGATATTGATAATGGTTTGGT
GGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGA
TAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTT
AGTTAACAAAACCAAAGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGC
ATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTT
CAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTAT
TCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAACCAACACTTTTGGA
ACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGT
ACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATT
CAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTC
AAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTAT
CGTTGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTT
AAATCCAAAATTACCAACTNCTNAAGNAATATAA

visiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*NKKK
XVNFTIXQIRSIMDRRENIKNMSVIAHVDHGKTTLTDSLIQRAGIIAXKVSGNAGYMSCR
PXEQLRGITIKSSSVSLHFEMPKDXKLPTGCSSHEFLINLIDSPGHVDFSSEVTAALRVT
DGALVV---

---AKSPNKHNRIYMKSEPLSSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNI
WSFGPEYKGPNVLVNKTKGVQYLHEIKDSMVSAFQWISNEGVLCGEKMRSIRFNVYDVML
HADAIHRGSGQFIPTTRRVMYASQLSAQPTLLEPMYLVDISTPESGISGIYSVLNRRRGV
VIGEERRIGTPLFNIKAYLPVMESFGLTADLRSSTAGQAFPQCVFDHWSSIGIVGQDKRS
TEVASSIRKRKGLNPKLPTXXXI*

Frame A:
visiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*NKKK
XVNFTIXQIRSIMDRRENIKNMSVIAHVDHGKTTLTDSLIQRAGIIAXKVSGNAGYMSCR
PXEQLRGITIKSSSVSLHFEMPKDXKLPTGCSSHEFLINLIDSPGHVDFSSEVTAALRVT
DGALVV---

---ckitk*tqsylhei*tiivrpn**y**wfggf*src*kscqlsl**tflg*e*cs*h
lviwsri*gskcfs*qnqrcsiss*n*rlygfsipmdlk*rcimw*eneidsfqrl*cna
scrcns*r*wsiysnnsscnvclptisstntfgtnvfg*y*ys*kwy*wyllctqs*krc
chw*rtsywysiiqyqslsscngiiwshc*ftfkycwssfpsmcf*slefnwyrwsr*ki
n*ssfin*kekrfkskitnxxxni

Frame B:
syq**illhiqnkinkt*tlffpyigqtliclctiilfkllllkfilktliskktktkkk
xsilqxikfvqlwivxkiskicllllmlimvkllllih*fkelallxirylvmqdichvv
xmnnyvvlp*shhlfhftlkcqkmxnyqlvvahtns*sillihqvmlilvlk*qqhfvlp
mvhw*l---

---AKSPNKHNRIYMKSEPLSSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNI
WSFGPEYKGPNVLVNKTKGVQYLHEIKDSMVSAFQWISNEGVLCGEKMRSIRFNVYDVML
HADAIHRGSGQFIPTTRRVMYASQLSAQPTLLEPMYLVDISTPESGISGIYSVLNRRRGV
VIGEERRIGTPLFNIKAYLPVMESFGLTADLRSSTAGQAFPQCVFDHWSSIGIVGQDKRS
TEVASSIRKRKGLNPKLPTXXXI*

Frame C:
hinnkyyyiyktkliklklyfsptlakl*fafvqlfslnsyy*nsf*klsflkklkqkkx
gqfynxsnsfnygssxkyqkyvcycsc*sw*nysy*finskswhycx*giw*criyvmss
x*titwyyhkviicftsl*nakr*xitnwl*ltrildqsy*ftrsc*f*f*snsstscyr
wcigsy---

---qnhqintivst*nlnhyrqt*lmilimvwwllikmlkvvpiisminilgirmmqltf
ghlvqnirvqmf*ltkpkvfnifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cf
mpmqfievvvnlfqqlvv*cmppnyqlnqhfwnqciwlilvllkvvlvvstlysiveevl
slvknvvlvlhysisklifl*wnhlvslliyvqvllvklslnvfliigvqlvslvkikdq
lk*lhqlerekv*iqnyqllkxy

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS180 (CHS180Q) /CSM/CH/CHS1-D/CHS180Q.Seq.d/                       2216   0.0
SHA639 (SHA639Q) /CSM/SH/SHA6-B/SHA639Q.Seq.d/                       1495   0.0
AHN643 (AHN643Q) /CSM/AH/AHN6-B/AHN643Q.Seq.d/                       1477   0.0
VHK348 (VHK348Q) /CSM/VH/VHK3-B/VHK348Q.Seq.d/                       1467   0.0
VHB815 (VHB815Q) /CSM/VH/VHB8-A/VHB815Q.Seq.d/                       1467   0.0
SHG365 (SHG365Q) /CSM/SH/SHG3-C/SHG365Q.Seq.d/                       1467   0.0
VHB107 (VHB107Q) /CSM/VH/VHB1-A/VHB107Q.Seq.d/                       1465   0.0
AHC347 (AHC347Q) /CSM/AH/AHC3-B/AHC347Q.Seq.d/                       1465   0.0
VHC477 (VHC477Q) /CSM/VH/VHC4-D/VHC477Q.Seq.d/                       1463   0.0
AHM892 (AHM892Q) /CSM/AH/AHM8-D/AHM892Q.Seq.d/                       1463   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   172   e-117  9
X15386|X15386.1 Dictyostelium discoideum H6 gene mRNA.            331   7e-91  2
AY310285|AY310285.1 Scutigera coleoptrata voucher Scol elongation factor 2 mRNA, partial cds.    66   2e-21  3
BI863585|BI863585.1 kx46a06.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   3e-21  4
BI863575|BI863575.1 kx45h07.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CHICK Q90705 ELONGATION FACTOR 2 ;, mRNA sequence.    66   4e-20  4
CO112841|CO112841.1 GR__Eb0049L19.r GR__Eb Gossypium raimondii cDNA clone GR__Eb0049L19 3', mRNA sequence.    74   2e-18  2
CO099234|CO099234.1 GR__Ea23P22.f GR__Ea Gossypium raimondii cDNA clone GR__Ea23P22 5', mRNA sequence.    74   2e-18  2
CO099235|CO099235.1 GR__Ea23P22.r GR__Ea Gossypium raimondii cDNA clone GR__Ea23P22 3', mRNA sequence.    74   2e-18  2
BI451383|BI451383.1 kx23e09.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   5e-18  3
L02417|L02417.1 Entamoeba histolytica translation elongation factor-2 (EF-2) mRNA, complete cds.    54   5e-17  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   303   9e-81
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   299   1e-79
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   299   2e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   299   2e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   299   2e-79
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   299   2e-79
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   299   2e-79
AK159514_1(AK159514|pid:none) Mus musculus osteoclast-like cell ...   299   2e-79
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   298   2e-79
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   298   2e-79

 psg: 0.80  gvh: 0.43  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.04
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for CHS180 is cyt

>CHS180F.Seq
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAA
ANGGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAA
AATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATT
CAAAGAGCTGGCATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGT
CCANATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAA
ATGCCAAAAGATGANAAATTACCAACTGGTTGTAGCTCACACGAATTCTTGATCAATCTT
ATTGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAGCAGCACTTCGTGTTACC
GATGGTGCATTGGTAGTTATNNNNNNNNNN

>CHS180Z.Seq
NNNNNNNNNNTGCAAAATCACCAAATAAACACAATCGTATCTACATGAAATCTGAACCAT
TATCGTCAGACCTAACTGATGATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTA
AAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACA
TTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGTG
TTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATG
AAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGC
TTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAA
TGTATGCCTCCCAACTATCAGCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATA
TTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTG
TTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTC
CTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTT
TCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCGTTGGTCAAGATAAAAGAT
CAACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTTAAATCCAAAATTACCAACTN
CTNAAGNAATATAA

>CHS180P.Seq
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAA
ANGGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAA
AATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATT
CAAAGAGCTGGCATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGT
CCANATGAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAA
ATGCCAAAAGATGANAAATTACCAACTGGTTGTAGCTCACACGAATTCTTGATCAATCTT
ATTGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAGCAGCACTTCGTGTTACC
GATGGTGCATTGGTAGTTAT----------TGCAAAATCACCAAATAAACACAATCGTAT
CTACATGAAATCTGAACCATTATCGTCAGACCTAACTGATGATATTGATAATGGTTTGGT
GGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGA
TAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTT
AGTTAACAAAACCAAAGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGC
ATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTT
CAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTAT
TCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAACCAACACTTTTGGA
ACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGT
ACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATT
CAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTC
AAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTAT
CGTTGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTT
AAATCCAAAATTACCAACTNCTNAAGNAATATAA