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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS166
Library CH
(Link to library)
Clone ID CHS166
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHS166P
(Link to Original site)
Representative DNA sequence 
>CHS166 (CHS166Q) /CSM/CH/CHS1-C/CHS166Q.Seq.d/
ATNATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAA
TTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTG
AAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATA
CAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAG
TATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATA
TGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTG
AATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAA
AAAXXXXXXXXXXCCATGTAAGAAAACTCAAACCTTCTNTACATACTCTGATAATCAACC
AGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATT
GGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGT
TACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGG
TAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGA
AAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGT
TGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGA
AAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAG
TGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGAT
GAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTAT
GCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAA
CAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTANCAAATAGAT
TTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATAT
sequence update 2002.10.25
Translated Amino Acid sequence 
xyc*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDT
ERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVE
FKGETKVFSPEEISSMVLLK---

---PCKKTQTFXTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVT
FDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVE
SKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMK
ALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisxk*is
*kiis**nq*sfsy
Translated Amino Acid sequence (All Frames) 
Frame A:
xiivkirvnik*lnikkchqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlli
qnv*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifkl
nskvklkfshqkkslqwys*k---

---PCKKTQTFXTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVT
FDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVE
SKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMK
ALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisxk*is
*kiis**nq*sfsy

Frame B:
xlllk*e*ilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*y
rtfnw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*
iqr*n*sfltrrnlfngtlk---

---hvrklkpsxhtliinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklh
lmlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivln
qrinwkimhsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*k
p*kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslxnrfl
kk**vskinkash

Frame C:
xyc*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDT
ERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVE
FKGETKVFSPEEISSMVLLK---

---m*ensnllyil**strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi
*c*ckwylkcis*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*i
ke*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*des
lrsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlxqidfl
knnklvksiklli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS166 (CHS166Q) /CSM/CH/CHS1-C/CHS166Q.Seq.d/                       2177   0.0
VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1481   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1481   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1481   0.0
VHP224 (VHP224Q) /CSM/VH/VHP2-A/VHP224Q.Seq.d/                       1481   0.0
VHO566 (VHO566Q) /CSM/VH/VHO5-C/VHO566Q.Seq.d/                       1481   0.0
VHO443 (VHO443Q) /CSM/VH/VHO4-B/VHO443Q.Seq.d/                       1481   0.0
VHN562 (VHN562Q) /CSM/VH/VHN5-C/VHN562Q.Seq.d/                       1481   0.0
VHK815 (VHK815Q) /CSM/VH/VHK8-A/VHK815Q.Seq.d/                       1481   0.0
VHH646 (VHH646Q) /CSM/VH/VHH6-B/VHH646Q.Seq.d/                       1481   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1481   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1473   0.0    1
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   252   e-124  6
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   252   e-115  8
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   145   4e-96  9
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 133   2e-93  6
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   133   3e-93  6
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          133   3e-91  6
M84652|M84652.1 Entamoeba heat shock protein (Hsp70) mRNA, complete cds.   172   3e-87  5
DQ060426|DQ060426.1 Cryptosporidium andersoni strain zzca heat shock protein 70-like gene, partial sequence.   127   7e-86  8
dna update 2005.11.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   376   e-103
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   376   e-103
EF506932_1(EF506932|pid:none) Fasciola hepatica heat shock prote...   275   3e-72
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           275   3e-72
EF506931_1(EF506931|pid:none) Fasciola gigantica heat shock prot...   273   6e-72
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   273   8e-72
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   273   1e-71
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   272   1e-71
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   272   1e-71
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   271   2e-71
protein update 2009. 4.13
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHS166 is cyt
5' end seq. ID CHS166F
5' end seq. 
>CHS166F.Seq
ATNATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAA
TTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTG
AAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATA
CAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAG
TATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATA
TGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTG
AATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAA
AAANNNNNNNNNN
Length of 5' end seq. 433
3' end seq. ID CHS166Z
3' end seq. 
>CHS166Z.Seq
NNNNNNNNNNCCATGTAAGAAAACTCAAACCTTCTNTACATACTCTGATAATCAACCAGG
TGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGG
TAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTAC
ATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAA
CAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAA
AATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGA
ATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAA
AGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGT
ACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAA
AGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCC
ACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAA
AGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTANCAAATAGATTTC
TTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATAT
Length of 3' end seq. 763
Connected seq. ID CHS166P
Connected seq. 
>CHS166P.Seq
ATNATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAA
TTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTG
AAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATA
CAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAG
TATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATA
TGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTG
AATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAA
AAA----------CCATGTAAGAAAACTCAAACCTTCTNTACATACTCTGATAATCAACC
AGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATT
GGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGT
TACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGG
TAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGA
AAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGT
TGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGA
AAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAG
TGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGAT
GAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTAT
GCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAA
CAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTANCAAATAGAT
TTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATAT
Length of connected seq. 1176
Full length Seq ID -
Full length Seq. -
Length of full length seq. -