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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHS140
Library CH
(Link to library)
Clone ID CHS140
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16405-1
Original site URL
Representative seq. ID CHS140P
(Link to Original site)
Representative DNA sequence 
>CHS140 (CHS140Q) /CSM/CH/CHS1-B/CHS140Q.Seq.d/
AATTTTTTTTTTAATCTTTTTTTATCTATTTAATAAAAATTAAATTTTCCTAAAATAATT
AAATTTCTCTTTTTTAATTTTCTTTTATAAAAATTATTAAAAAGCCAAATTCAAAATAAA
AAAAATCATTGATAAAAATGGAAAAGAAAATAATATTACTGATTTTATTTTTGTTCTTTA
TATCAGGATATTCAATGAATCCTCCAACACCAATAGATGCAATATATGATGATAAATCAT
TTACATTAATTTTTAATAGTAATCTCCCATATTCTACCCGTTTAATTTTGTATAAAAACG
AAAAAGAACCCAGAACTGAAATGGCACCAAATTATTTCAATTGTTCTCTAGTTGATGGAN
ANAGACATTGTTTATTCCATTCANATGAACCATTCTCAANATTATGGGGTAGTATANACA
GTAAGGTATGTGTTANANATAAATCAAATACTGTTGAGGATTGTACATTTGATGCTTCGG
GTTTAGTATATTATCCAAAAGTATACAATTTGAAATATAGTAAAAAACCAAAAACATCTG
GTGXXXXXXXXXXGGTCTTATCACAGTTTCTGGTAAGAATTTATTGACAAGTGACAAAGA
ATTCAAAGTTAATGTAAAATCAAATAATAAAAATACCACAGTATTTGCTCAAGATGAAAA
GATATTAATAGTTAGAGATGAAAGTAGAGAGAGTTCACTCTTTGTTACAACATTCATTGG
AGTTCGTTCAGGACCAAGTACAACATTGACCTATATAAAACCAATGATTTCAGAAATTCC
AACCATTGAAAATAAAATTGAAAAAGGTATTTTAGCAATAATTCGTGGTTATAGTTTTAC
AGATATTCTCAATGCAAGCTTAACAGTTTCATCAGAAACTGTTCCTCTTTCATGTAATTT
AGAATGTAGTTTATCACCAAATGAAATACTTGATGATAGTGATTCAAGTGAAACTAATAT
TACAAATAGTAATACTGATTGTTTATCATGCCATTCCGGTCCAAGTGTGAAAAATACATC
AGGAGTATTATATCTCCTATTTAACTCAACATCATTTCAATATAATGTCACAATTGAAGA
AATTAAATTATCTCCATCACCAAACGTTAGTCAAAGAGATGTTGAAACTAAATCATCCAA
ACCATCAAATGGTTTAATAATAGGTTCTACAATTGGTAGTGTTGGTGGTGCTCTTGCTAT
CGGTGCTCTTGCCTATTACTTTAAAATACCTTTTCGTGNNAAAAAATTTATAGGTAAAAA
A
sequence update 2002.10.25
Translated Amino Acid sequence 
fff*sffiylikikfs*nn*islf*fsfikiikkpnsk*KKSLIKMEKKIILLILFLFFI
SGYSMNPPTPIDAIYDDKSFTLIFNSNLPYSTRLILYKNEKEPRTEMAPNYFNCSLVDGX
RHCLFHSXEPFSXLWGSIXSKVCVXXKSNTVEDCTFDASGLVYYPKVYNLKYSKKPKTSG
---

---GLITVSGKNLLTSDKEFKVNVKSNNKNTTVFAQDEKILIVRDESRESSLFVTTFIGV
RSGPSTTLTYIKPMISEIPTIENKIEKGILAIIRGYSFTDILNASLTVSSETVPLSCNLE
CSLSPNEILDDSDSSETNITNSNTDCLSCHSGPSVKNTSGVLYLLFNSTSFQYNVTIEEI
KLSPSPNVSQRDVETKSSKPSNGLIIGSTIGSVGGALAIGALAYYFKIPFRXKKFIGKK
Translated Amino Acid sequence (All Frames) 
Frame A:
nfffnlflsi**klnfpkiikflffnfll*kllksqiqnkknh**kwkrk*yy*fyfcsl
yqdiq*ilqhq*mqymminhlh*flivishilpv*fciktkknpelkwhqiisivl*lmx
xdivysixmnhsqxygvvxtvryvlxinqillrivhlmlrv*yiiqkyti*nivknqkhl
v---

---GLITVSGKNLLTSDKEFKVNVKSNNKNTTVFAQDEKILIVRDESRESSLFVTTFIGV
RSGPSTTLTYIKPMISEIPTIENKIEKGILAIIRGYSFTDILNASLTVSSETVPLSCNLE
CSLSPNEILDDSDSSETNITNSNTDCLSCHSGPSVKNTSGVLYLLFNSTSFQYNVTIEEI
KLSPSPNVSQRDVETKSSKPSNGLIIGSTIGSVGGALAIGALAYYFKIPFRXKKFIGKK

Frame B:
iffliffylfnkn*iflk*lnfsfliffykny*kakfkikkiidkngkennitdfifvly
irifnessntnrcni***iiyinf***spifypfnfv*krkrtqn*ngtklfqlfss*wx
xtlfipfx*tilximg*yxq*gmcxx*ikyc*glyi*cfgfsilsksiqfei**ktkniw
---

---vlsqflvriy*qvtknsklm*nqiikipqyllkmkry**lemkvervhsllqhslef
vqdqvqh*pi*nq*fqkfqplkiklkkvf*q*fvvivlqifsmqa*qfhqklflfhvi*n
vvyhqmkylmiviqvklilqivilivyhaipvqv*kihqeyyisyltqhhfnimsqlkkl
nylhhqtlvkemlklnhpnhqmv***vlqlvvlvvlllsvllpitlkylfvxknl*vk

Frame C:
fff*sffiylikikfs*nn*islf*fsfikiikkpnsk*KKSLIKMEKKIILLILFLFFI
SGYSMNPPTPIDAIYDDKSFTLIFNSNLPYSTRLILYKNEKEPRTEMAPNYFNCSLVDGX
RHCLFHSXEPFSXLWGSIXSKVCVXXKSNTVEDCTFDASGLVYYPKVYNLKYSKKPKTSG
---

---syhsfw*efidk*qriqs*ckik**kyhsicsr*kdins*r*k*reftlcynihwss
frtkynidlyktndfrnsnh*k*n*kryfsnnswl*fyrysqcklnsfirncssfm*frm
*fitk*nt****fk*n*yyk**y*lfimpfrskcekyirsiispi*lniisi*chn*rn*
iisitkr*skrc*n*iiqtikwfnnrfynw*cwwcscyrcsclll*ntfsxxkiyr*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS140 (CHS140Q) /CSM/CH/CHS1-B/CHS140Q.Seq.d/                       1392   0.0
AHK465 (AHK465Q) /CSM/AH/AHK4-C/AHK465Q.Seq.d/                       1374   0.0
AHK212 (AHK212Q) /CSM/AH/AHK2-A/AHK212Q.Seq.d/                       1374   0.0
AHF119 (AHF119Q) /CSM/AH/AHF1-A/AHF119Q.Seq.d/                       1374   0.0
AHA415 (AHA415Q) /CSM/AH/AHA4-A/AHA415Q.Seq.d/                       1374   0.0
AHA165 (AHA165Q) /CSM/AH/AHA1-C/AHA165Q.Seq.d/                       1374   0.0
AHM883 (AHM883Q) /CSM/AH/AHM8-D/AHM883Q.Seq.d/                       1372   0.0
SHD571 (SHD571Q) /CSM/SH/SHD5-C/SHD571Q.Seq.d/                       1370   0.0
AHL692 (AHL692Q) /CSM/AH/AHL6-D/AHL692Q.Seq.d/                       1370   0.0
AHL221 (AHL221Q) /CSM/AH/AHL2-A/AHL221Q.Seq.d/                       1370   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF258615|AF258615.1 Dictyostelium discoideum LagC protein (lagC) gene, complete cds.  1384   0.0    1
U09478|U09478.1 Dictyostelium discoideum KAx-3 LagC protein (lagC) mRNA, complete cds.  1384   0.0    1
AB032076|AB032076.1 Dictyostelium discoideum mRNA for lagC2, complete cds.    80   4e-39  6
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    66   2e-06  1
AE009951|AE009951.2 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, complete genome.    34   0.013  19
AC094308|AC094308.10 Rattus norvegicus clone CH230-3G2, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    50   0.11   1
AL935175|AL935175.6 Mouse DNA sequence from clone RP23-397E13 on chromosome 2.    38   0.13   5
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    32   0.21   12
CT025578|CT025578.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    36   0.25   2
BX255889|BX255889.7 Zebrafish DNA sequence from clone CH211-270O7 in linkage group 17.    36   0.25   2
dna update 2005.11.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A54280(A54280) cell differentiation protein LagC precursor - sli...   394   e-108
FJ374927_1(FJ374927|pid:none) Dictyostelium discoideum strain QS...   385   e-105
FJ374925_1(FJ374925|pid:none) Dictyostelium discoideum strain QS...   385   e-105
FJ374948_1(FJ374948|pid:none) Dictyostelium discoideum strain QS...   385   e-105
FJ374923_1(FJ374923|pid:none) Dictyostelium discoideum strain QS...   385   e-105
FJ374941_1(FJ374941|pid:none) Dictyostelium discoideum strain QS...   384   e-105
FJ374933_1(FJ374933|pid:none) Dictyostelium discoideum strain QS...   381   e-104
FJ374928_1(FJ374928|pid:none) Dictyostelium discoideum strain QS...   369   e-100
FJ374930_1(FJ374930|pid:none) Dictyostelium discoideum strain QS...   369   e-100
FJ374951_1(FJ374951|pid:none) Dictyostelium discoideum strain QS...   369   e-100
protein update 2009. 4.13
PSORT 

 psg: 1.02  gvh: 0.77  alm: 0.34  top: 0.07  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial

>> prediction for CHS140 is exc
5' end seq. ID CHS140F
5' end seq. 
>CHS140F.Seq
AATTTTTTTTTTAATCTTTTTTTATCTATTTAATAAAAATTAAATTTTCCTAAAATAATT
AAATTTCTCTTTTTTAATTTTCTTTTATAAAAATTATTAAAAAGCCAAATTCAAAATAAA
AAAAATCATTGATAAAAATGGAAAAGAAAATAATATTACTGATTTTATTTTTGTTCTTTA
TATCAGGATATTCAATGAATCCTCCAACACCAATAGATGCAATATATGATGATAAATCAT
TTACATTAATTTTTAATAGTAATCTCCCATATTCTACCCGTTTAATTTTGTATAAAAACG
AAAAAGAACCCAGAACTGAAATGGCACCAAATTATTTCAATTGTTCTCTAGTTGATGGAN
ANAGACATTGTTTATTCCATTCANATGAACCATTCTCAANATTATGGGGTAGTATANACA
GTAAGGTATGTGTTANANATAAATCAAATACTGTTGAGGATTGTACATTTGATGCTTCGG
GTTTAGTATATTATCCAAAAGTATACAATTTGAAATATAGTAAAAAACCAAAAACATCTG
GTGNNNNNNNNNN
Length of 5' end seq. 553
3' end seq. ID CHS140Z
3' end seq. 
>CHS140Z.Seq
NNNNNNNNNNGGTCTTATCACAGTTTCTGGTAAGAATTTATTGACAAGTGACAAAGAATT
CAAAGTTAATGTAAAATCAAATAATAAAAATACCACAGTATTTGCTCAAGATGAAAAGAT
ATTAATAGTTAGAGATGAAAGTAGAGAGAGTTCACTCTTTGTTACAACATTCATTGGAGT
TCGTTCAGGACCAAGTACAACATTGACCTATATAAAACCAATGATTTCAGAAATTCCAAC
CATTGAAAATAAAATTGAAAAAGGTATTTTAGCAATAATTCGTGGTTATAGTTTTACAGA
TATTCTCAATGCAAGCTTAACAGTTTCATCAGAAACTGTTCCTCTTTCATGTAATTTAGA
ATGTAGTTTATCACCAAATGAAATACTTGATGATAGTGATTCAAGTGAAACTAATATTAC
AAATAGTAATACTGATTGTTTATCATGCCATTCCGGTCCAAGTGTGAAAAATACATCAGG
AGTATTATATCTCCTATTTAACTCAACATCATTTCAATATAATGTCACAATTGAAGAAAT
TAAATTATCTCCATCACCAAACGTTAGTCAAAGAGATGTTGAAACTAAATCATCCAAACC
ATCAAATGGTTTAATAATAGGTTCTACAATTGGTAGTGTTGGTGGTGCTCTTGCTATCGG
TGCTCTTGCCTATTACTTTAAAATACCTTTTCGTGNNAAAAAATTTATAGGTAAAAAA
Length of 3' end seq. 718
Connected seq. ID CHS140P
Connected seq. 
>CHS140P.Seq
AATTTTTTTTTTAATCTTTTTTTATCTATTTAATAAAAATTAAATTTTCCTAAAATAATT
AAATTTCTCTTTTTTAATTTTCTTTTATAAAAATTATTAAAAAGCCAAATTCAAAATAAA
AAAAATCATTGATAAAAATGGAAAAGAAAATAATATTACTGATTTTATTTTTGTTCTTTA
TATCAGGATATTCAATGAATCCTCCAACACCAATAGATGCAATATATGATGATAAATCAT
TTACATTAATTTTTAATAGTAATCTCCCATATTCTACCCGTTTAATTTTGTATAAAAACG
AAAAAGAACCCAGAACTGAAATGGCACCAAATTATTTCAATTGTTCTCTAGTTGATGGAN
ANAGACATTGTTTATTCCATTCANATGAACCATTCTCAANATTATGGGGTAGTATANACA
GTAAGGTATGTGTTANANATAAATCAAATACTGTTGAGGATTGTACATTTGATGCTTCGG
GTTTAGTATATTATCCAAAAGTATACAATTTGAAATATAGTAAAAAACCAAAAACATCTG
GTG----------GGTCTTATCACAGTTTCTGGTAAGAATTTATTGACAAGTGACAAAGA
ATTCAAAGTTAATGTAAAATCAAATAATAAAAATACCACAGTATTTGCTCAAGATGAAAA
GATATTAATAGTTAGAGATGAAAGTAGAGAGAGTTCACTCTTTGTTACAACATTCATTGG
AGTTCGTTCAGGACCAAGTACAACATTGACCTATATAAAACCAATGATTTCAGAAATTCC
AACCATTGAAAATAAAATTGAAAAAGGTATTTTAGCAATAATTCGTGGTTATAGTTTTAC
AGATATTCTCAATGCAAGCTTAACAGTTTCATCAGAAACTGTTCCTCTTTCATGTAATTT
AGAATGTAGTTTATCACCAAATGAAATACTTGATGATAGTGATTCAAGTGAAACTAATAT
TACAAATAGTAATACTGATTGTTTATCATGCCATTCCGGTCCAAGTGTGAAAAATACATC
AGGAGTATTATATCTCCTATTTAACTCAACATCATTTCAATATAATGTCACAATTGAAGA
AATTAAATTATCTCCATCACCAAACGTTAGTCAAAGAGATGTTGAAACTAAATCATCCAA
ACCATCAAATGGTTTAATAATAGGTTCTACAATTGGTAGTGTTGGTGGTGCTCTTGCTAT
CGGTGCTCTTGCCTATTACTTTAAAATACCTTTTCGTGNNAAAAAATTTATAGGTAAAAA
A
Length of connected seq. 1251
Full length Seq ID -
Full length Seq. -
Length of full length seq. -