>CHS131 (CHS131Q) /CSM/CH/CHS1-B/CHS131Q.Seq.d/
XXXXXXXXXXCAATCGATGAAGCCGTTGAATTGGCTCAATCATCAGATGTTGTNGTCGTT
GTAATTGGTGAACTTCCAGAAGCTGAAACTCCAGGTGATATTTATGATCTCTCTATGGAT
CCAAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATT
TTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTT
TTAATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGT
AATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGT
GTTCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGT
GATGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCA
ATCTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTC
ACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGAT
CTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTT
ATGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATT
GGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTA

---IDEAVELAQSSDVVVVVIGELPEAETPGDIYDLSMDPNEVLLLQQLVDTGKPVVLIL
VEARPRILPPDLVYSCAAVLMAYLPGSEGGKPIANILMGNVNPSGRLPLTYPGTTGDIGV
PYYHKYSENGVTTPLFQFGDGLSYTTFNYTNLACSNCKPISGQSGNYTGVLGQSYTFTVT
VTNNGNVQGKDSVLLYLSDLWAQVTPEVKMLRGFQKVDLMPAKSQQISFTLNAYEFSFIG
VDNKITLESVNSSL

Frame A:
---nr*sr*igsiirccxrcnw*tsrs*nsr*yl*slygsk*stlittig*hw*tssfnf
g*sssknfttrsrlqlccsfnglspr**rw*tnrqhshg*cksiwsssfnlsryyw*yrc
silpqiq*kwchhstlpiw*wsflyhf*lyqsrl*ql*tnlwsiw*lhwcfrsilyfhch
chkqw*rsr*rlcslisl*slgssysrs*nvkrfpks*syas*istnlfypqcl*illyw
cr**nyfrigqfii


Frame B:
---IDEAVELAQSSDVVVVVIGELPEAETPGDIYDLSMDPNEVLLLQQLVDTGKPVVLIL
VEARPRILPPDLVYSCAAVLMAYLPGSEGGKPIANILMGNVNPSGRLPLTYPGTTGDIGV
PYYHKYSENGVTTPLFQFGDGLSYTTFNYTNLACSNCKPISGQSGNYTGVLGQSYTFTVT
VTNNGNVQGKDSVLLYLSDLWAQVTPEVKMLRGFQKVDLMPAKSQQISFTLNAYEFSFIG
VDNKITLESVNSSL


Frame C:
---smkplnwlnhqmlxsl*lvnfqklklqvifmislwiqmkysyynnwltlvnq*f*fw
lklvqefyhqisstvvlqf*wlisqvvkvvnqsptfswvm*ihlvvfl*liqvllvisvf
hittntvkmvsplhssnlvmvfliplltipispvaivnqslvnlvttlvf*vnpilslsl
sqtmvtfkvktlfsyislifglkllqklkc*evskklilcqlnlnksllpsmpmnspllv
siikll*nrsihh

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD112 (VHD112Q) /CSM/VH/VHD1-A/VHD112Q.Seq.d/                       1491   0.0
VHB282 (VHB282Q) /CSM/VH/VHB2-D/VHB282Q.Seq.d/                       1491   0.0
VHA583 (VHA583Q) /CSM/VH/VHA5-D/VHA583Q.Seq.d/                       1491   0.0
VHA533 (VHA533Q) /CSM/VH/VHA5-B/VHA533Q.Seq.d/                       1491   0.0
CHS391 (CHS391Q) /CSM/CH/CHS3-D/CHS391Q.Seq.d/                       1491   0.0
CHS131 (CHS131Q) /CSM/CH/CHS1-B/CHS131Q.Seq.d/                       1491   0.0
CHL451 (CHL451Q) /CSM/CH/CHL4-C/CHL451Q.Seq.d/                       1491   0.0
CHF295 (CHF295Q) /CSM/CH/CHF2-D/CHF295Q.Seq.d/                       1491   0.0
VHP578 (VHP578Q) /CSM/VH/VHP5-D/VHP578Q.Seq.d/                       1489   0.0
VHJ810 (VHJ810Q) /CSM/VH/VHJ8-A/VHJ810Q.Seq.d/                       1489   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L21014|L21014.1 Dictyostelium discoideum beta-glucosidase mRNA, complete cds.   977   0.0    2
AL645696|AL645696.30 Mouse DNA sequence from clone RP23-429E18 on chromosome 1.    38   0.41   3
AC101800|AC101800.8 Mus musculus chromosome 1, clone RP24-219K10, complete sequence.    38   0.49   3
BX385390|BX385390.2 human full-length cDNA 5-PRIME end of clone CS0DL010YP21 of B CELLS  (RAMOS CELL LINE) COT 25-NORMALIZED of Homo sapiens (human).    36   0.96   2
BC066922|BC066922.1 Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 2 pseudogene 3, mRNA (cDNA clone MGC:87149 IMAGE:5273554), complete cds.    36   1.6    2
AC098252|AC098252.4 Rattus norvegicus clone CH230-1H24, *** SEQUENCING IN PROGRESS ***, 145 unordered pieces.    32   1.9    2
AC110686|AC110686.4 Rattus norvegicus clone CH230-197B1, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    32   2.0    2
BU282816|BU282816.1 603863781F1 CSEQCHN54 Gallus gallus cDNA clone ChEST879a22 5', mRNA sequence.    34   2.4    2
AJ133812|AJ133812.1 Homo sapiens mRNA for inhibitor 2 of protein phosphatase 1.    36   2.4    2
BU109938|BU109938.1 603126903F1 CSEQCHL13 Gallus gallus cDNA clone ChEST97f2 5', mRNA sequence.    34   2.6    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   466   e-130
CP000159_2777(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   177   3e-43
CP000383_3519(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   160   4e-38
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...   157   3e-37
CP000383_2243(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   149   7e-35
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   145   1e-33
CP000685_3497(CP000685|pid:none) Flavobacterium johnsoniae UW101...   135   2e-30
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...   135   2e-30
CP000964_1565(CP000964|pid:none) Klebsiella pneumoniae 342, comp...   130   6e-29
CP001251_792(CP001251|pid:none) Dictyoglomus turgidum DSM 6724, ...   130   6e-29

 psg: 0.75  gvh: 0.53  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for CHS131 is cyt

>CHS131Z.Seq
NNNNNNNNNNCAATCGATGAAGCCGTTGAATTGGCTCAATCATCAGATGTTGTNGTCGTT
GTAATTGGTGAACTTCCAGAAGCTGAAACTCCAGGTGATATTTATGATCTCTCTATGGAT
CCAAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATT
TTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTT
TTAATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGT
AATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGT
GTTCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGT
GATGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCA
ATCTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTC
ACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGAT
CTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTT
ATGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATT
GGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTA