>CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS124Q.Seq.d/
TAAAATTAAAGAAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATA
GCAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTT
TAGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTG
TTAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAAT
TTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAG
TTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAAT
TCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAG
TGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTT
TTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAATCACCGATACAAATGGTGAGA
ATCCAAATGCTTATATGATCAGTTCAATCAGTCAAGAXXXXXXXXXXCATCCAAAANCAA
GCTGCCAATGTTATTATTCCAGATTTCACTCCAAAGAAGGTTAAGATTCAAACCTCTGAA
AATGAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTGATGCTGAAGATGATCAA
TGTGATACCATTTTATCACAACTTCCACAACCAAGTGAAATGGCTGGTTACAAAATTAAC
TCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACC
TCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGT
ATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTGCTGGTTTCGTT
TGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTT
ATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAA
ATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACT
CTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCT
TGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTA
AAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAG
AAATATTTATTACTGAAATTTGTTGCAA

KLKKPMDVEQEPKIDDALYSRQLYALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGV
KSVTLYDKELVEIKDLSSQFYFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEF
LKKFNVVVLANQPLALQLKVNEFCHANKIHFISVETRGVFGQLFNDFGEQFTITDTNGEN
PNAYMISSISQ---

---IQXQAANVIIPDFTPKKVKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSE
MAGYKINSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTT
AVVAGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRF
DVDGDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSK
KPLPKDKKYLLLKFVA

Frame A:
*n*rnqwm*nknqklmmlyiadnfmhylmkq*kklhqlqf*l*vykvlvlkllki*v*qv
lnllhcmikn*lklkicllnfifhqnklvklveqmhvskkl*i*iimlelmyimen*vmn
s*rnsml*y*pinh*hynsk*mnfvmqikfislvlkpevflvnysmilvnnlqspiqmvr
iqmli*svqsvk---

---hpkxscqcyysrfhskeg*dsnl*k*tssifkhstsww*c*r*sm*yhfittsttk*
ngwlqn*lysi*kg**yqssyrfhyrylkfksyqlcnqss**t*dqryrr*nhssfsyhy
crscwfrlyrid*sysk*sfgei*ehlyefrypilwfr*tncrpkeqns*rldldtlgsf
rc*w*yhsqgvfrsl*ketwfgyfnall*sytficslh**en*rgkikdenlttl*nplk
etitkr*eifiteicc

Frame B:
kiketngcrtrtkn**cfi*qttlciis*nneknyinfsfscrftrswy*nc*rfkfsrc
*icyiv**ris*n*rfvfsilfftrtsw*sw*srcmfpkscrfk*lc*n*ct*wrik**i
lkeiqccsisqstisittqse*ilsck*nsfh*c*nqrcfwsiiq*fw*tiynhrykw*e
skclydqfnqsr---

---IQXQAANVIIPDFTPKKVKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSE
MAGYKINSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTT
AVVAGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRF
DVDGDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSK
KPLPKDKKYLLLKFVA

Frame C:
KLKKPMDVEQEPKIDDALYSRQLYALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGV
KSVTLYDKELVEIKDLSSQFYFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEF
LKKFNVVVLANQPLALQLKVNEFCHANKIHFISVETRGVFGQLFNDFGEQFTITDTNGEN
PNAYMISSISQ---

---skxklpmllfqislqrrlrfkplkmnqlhlqtlnklvvmlkminvipfyhnfhnqvk
wlvtkltlfnlkrmmipiiisislplpqi*elptmqsvqlinirpkvspvksfql*lpll
p*llvsfvsn*lklfkiklwrnirapl*i*vshslvslnqlppqrtkfvkaglghfgivs
mlmvislsrsf*islkrnmvwifqcslvklhfymlsslirklkrkd*r*kshnsmkpsqr
nhyqkirniyy*nllq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS124Q.Seq.d/                       2565   0.0
SHD220 (SHD220Q) /CSM/SH/SHD2-A/SHD220Q.Seq.d/                       1483   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1481   0.0
VHA132 (VHA132Q) /CSM/VH/VHA1-B/VHA132Q.Seq.d/                       1481   0.0
SHA307 (SHA307Q) /CSM/SH/SHA3-A/SHA307Q.Seq.d/                       1473   0.0
AHQ886 (AHQ886Q) /CSM/AH/AHQ8-D/AHQ886Q.Seq.d/                       1473   0.0
AHN324 (AHN324Q) /CSM/AH/AHN3-A/AHN324Q.Seq.d/                       1473   0.0
VHA328 (VHA328Q) /CSM/VH/VHA3-B/VHA328Q.Seq.d/                       1471   0.0
SHC572 (SHC572Q) /CSM/SH/SHC5-C/SHC572Q.Seq.d/                       1471   0.0
CHQ706 (CHQ706Q) /CSM/CH/CHQ7-A/CHQ706Q.Seq.d/                       1467   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ930776|AZ930776.1 474.dhz58e03.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomycesunisporus genomic clone 474.dhz58e03.s1, genomic survey sequence.    68   7e-13  3
CB933531|CB933531.1 ri31b11.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodi cDNA 5' similar to TR:Q27481 Q27481 C47E12.5 PROTEIN. [1] ;, mRNA sequence.    64   4e-12  2
DR174736|DR174736.1 HR0003_M02:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   3e-06  2
DR174741|DR174741.1 HR0003_M08:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   3e-06  2
DR174755|DR174755.1 HR0003_N01:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   4e-06  2
AC134210|AC134210.1 Rattus norvegicus clone CH230-197N6, *** SEQUENCING IN PROGRESS ***, 60 unordered pieces.    58   5e-04  1
AC108277|AC108277.6 Rattus norvegicus clone CH230-27E16, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    58   5e-04  1
BZ303839|BZ303839.1 KD2618.q1 Kluyveromyces delphensis Random Genomic Library Kluyveromyces delphensis genomic clone KD2618, DNA sequence.    48   0.002  2
DR028930|DR028930.1 bda010063_A05_11.b1_024.ab1 Antrodia cinnamomea cDNA Library Antrodia cinnamomea cDNA, mRNA sequence.    52   0.003  2
AL403306|AL403306.1 T3 end of clone AT0AA007G10 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    44   0.003  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   469   e-131
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   249   2e-64
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   238   4e-61
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   232   2e-59
AE016819_666(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   230   7e-59
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   228   3e-58
BX908809_46(BX908809|pid:none) Neurospora crassa DNA linkage gro...   228   4e-58
CR382122_592(CR382122|pid:none) Kluyveromyces lactis strain NRRL...   228   4e-58
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   226   1e-57
AM270347_41(AM270347|pid:none) Aspergillus niger contig An15c020...   225   2e-57

 psg: 0.54  gvh: 0.52  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.20  mip: 0.00
 nuc: 0.14  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for CHS124 is cyt

>CHS124F.Seq
TAAAATTAAAGAAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATA
GCAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTT
TAGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTG
TTAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAAT
TTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAG
TTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAAT
TCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAG
TGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTT
TTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAATCACCGATACAAATGGTGAGA
ATCCAAATGCTTATATGATCAGTTCAATCAGTCAAGANNNNNNNNNN

>CHS124Z.Seq
NNNNNNNNNNCATCCAAAANCAAGCTGCCAATGTTATTATTCCAGATTTCACTCCAAAGA
AGGTTAAGATTCAAACCTCTGAAAATGAACCAGCTCCATCTTCAAACACTCAACAAGCTG
GTGGTGATGCTGAAGATGATCAATGTGATACCATTTTATCACAACTTCCACAACCAAGTG
AAATGGCTGGTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATC
ATATCGATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAG
CTGATAAACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTA
CTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTT
TGGAGAAATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAAC
CAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTT
TCGATGTTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATG
GTTTGGATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATA
AGAAAACTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAA
AGAAACCATTACCAAAAGATAAGAAATATTTATTACTGAAATTTGTTGCAA

>CHS124P.Seq
TAAAATTAAAGAAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATA
GCAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTT
TAGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTG
TTAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAAT
TTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAG
TTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAAT
TCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAG
TGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTT
TTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAATCACCGATACAAATGGTGAGA
ATCCAAATGCTTATATGATCAGTTCAATCAGTCAAGA----------CATCCAAAANCAA
GCTGCCAATGTTATTATTCCAGATTTCACTCCAAAGAAGGTTAAGATTCAAACCTCTGAA
AATGAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTGATGCTGAAGATGATCAA
TGTGATACCATTTTATCACAACTTCCACAACCAAGTGAAATGGCTGGTTACAAAATTAAC
TCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACC
TCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGT
ATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTGCTGGTTTCGTT
TGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTT
ATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAA
ATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACT
CTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCT
TGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTA
AAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAG
AAATATTTATTACTGAAATTTGTTGCAA