>CHS103 (CHS103Q) /CSM/CH/CHS1-A/CHS103Q.Seq.d/
XXXXXXXXXXGAAAGATTGTTATGCGATGAACAATACATTGTATTGCAAAGAGTGTAAAT
TCAATTGTCATTATTCAAAACATATGCATATCACATATGAAACATTTTTAATTAAAAAGG
AAATGGAAAACGAAGATGTTAAAAAACAAATTGAAGAAGGAATTGATGCAACATCTTTGA
AAAGACAATGGGTTGAATCCTTAGAGAAAGAAATTAAGGAAAATACTGATGAGAAAAATG
AAATACTTAAAATTAGCTCACAACTTTCATACTTTTTAAAAGAAAATTGCATTACAACAT
ACCATGATACAGTCATTGAATATTTGAAAATTTGTATTGAAAATGAAGAACTCAAATCAG
ATAAATCAGGTAAAAAATCAATGGAAGATAATTTAAAGTTTTTCCAAGATCAACATGATT
ATTTTCAAAAAAATCTTTCCAACCAACAAATCCAAAAAATCACAACAAAAGATGTCCCAA
TTCTCATTGAAAAATTAAAAAACCTCAAAGAAAATGGTAAAACACTTAGAGAATGCATCG
AAGCTAATGTAAATGAACAATTAAAAACAGGCTTCAATCCAATTGTTGTCACAAAAAATA
CAAATCATTCAAAGGAATGGGGTTTTATTTC

---KDCYAMNNTLYCKECKFNCHYSKHMHITYETFLIKKEMENEDVKKQIEEGIDATSLK
RQWVESLEKEIKENTDEKNEILKISSQLSYFLKENCITTYHDTVIEYLKICIENEELKSD
KSGKKSMEDNLKFFQDQHDYFQKNLSNQQIQKITTKDVPILIEKLKNLKENGKTLRECIE
ANVNEQLKTGFNPIVVTKNTNHSKEWGFI

Frame A:
---KDCYAMNNTLYCKECKFNCHYSKHMHITYETFLIKKEMENEDVKKQIEEGIDATSLK
RQWVESLEKEIKENTDEKNEILKISSQLSYFLKENCITTYHDTVIEYLKICIENEELKSD
KSGKKSMEDNLKFFQDQHDYFQKNLSNQQIQKITTKDVPILIEKLKNLKENGKTLRECIE
ANVNEQLKTGFNPIVVTKNTNHSKEWGFI


Frame B:
---kivmr*tihciaksvnsiviiqnicishmkhf*lkrkwktkmlknklkkelmqhl*k
dnglnp*rkklrkilmrkmkylklahnfhtf*kkialqhtmiqslni*kfvlkmknsnqi
nqvknqwkii*sfskinmiifkkifptnkskksqqkmsqfslkn*ktskkmvkhlenask
lm*mnn*kqasiqllsqkiqiiqrngvlf


Frame C:
---rllcdeqyivlqrv*iqlslfktyayhi*nifn*kgngkrrc*ktn*rrn*cnifek
tmg*ilrern*gky**ek*nt*n*lttfilfkrklhynip*ysh*ifenly*k*rtqir*
ir*kingr*fkvfprst*lfskksfqptnpknhnkrcpnsh*kikkpqrkw*nt*rmhrs
*ck*tiknrlqsncchkkyksfkgmgfyf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL509 (SHL509Q) /CSM/SH/SHL5-A/SHL509Q.Seq.d/                        906   0.0
SHL413 (SHL413Q) /CSM/SH/SHL4-A/SHL413Q.Seq.d/                        906   0.0
SHL381 (SHL381Q) /CSM/SH/SHL3-D/SHL381Q.Seq.d/                        906   0.0
SHL364 (SHL364Q) /CSM/SH/SHL3-C/SHL364Q.Seq.d/                        906   0.0
SHK833 (SHK833Q) /CSM/SH/SHK8-B/SHK833Q.Seq.d/                        906   0.0
SHK526 (SHK526Q) /CSM/SH/SHK5-B/SHK526Q.Seq.d/                        906   0.0
SHK473 (SHK473Q) /CSM/SH/SHK4-D/SHK473Q.Seq.d/                        906   0.0
SHK304 (SHK304Q) /CSM/SH/SHK3-A/SHK304Q.Seq.d/                        906   0.0
SHJ682 (SHJ682Q) /CSM/SH/SHJ6-D/SHJ682Q.Seq.d/                        906   0.0
SHJ586 (SHJ586Q) /CSM/SH/SHJ5-D/SHJ586Q.Seq.d/                        906   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC171368|AC171368.2 Cavia porcellus clone CH234-176E17, WORKING DRAFT SEQUENCE, 3 ordered pieces.    42   0.029  5
BA000021|BA000021.3 Wigglesworthia glossinidia endosymbiont of Glossina brevipalpis DNA, complete genome.    32   0.12   14
AC142246|AC142246.1 Mus musculus clone RP23-429J8, WORKING DRAFT SEQUENCE, 5 unordered pieces.    38   0.16   7
AC108471|AC108471.3 Homo sapiens BAC clone RP11-539G18 from 4, complete sequence.    42   0.20   3
CR382401|CR382401.1 Plasmodium falciparum chromosome 6, complete sequence; segment 4/5.    36   0.21   8
AW013744|AW013744.1 BSBmMFSZ22G10SK Brugia malayi microfilaria cDNA (SAW94LS-BmMf) Brugia malayi cDNA clone BSBmMFSZ22G10 5', mRNA sequence.    48   0.22   1
AL897247|AL897247.2 Xenopus tropicalis EST, clone TEgg013f13 3'.    48   0.22   1
AC111204|AC111204.5 Rattus norvegicus clone CH230-31G2, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    48   0.22   1
AQ101588|AQ101588.1 HS_2235_B1_C06_MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2235 Col=11 Row=F, genomic survey sequence.    48   0.22   1
AQ074823|AQ074823.1 CIT-HSP-2351E18.TF CIT-HSP Homo sapiens genomic clone 2351E18, genomic survey sequence.    48   0.22   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC114263_3(AC114263|pid:none) Dictyostelium discoideum chromosom...    66   9e-10
CP000025_577(CP000025|pid:none) Campylobacter jejuni RM1221, com...    46   7e-04
BC152666_1(BC152666|pid:none) Danio rerio hypothetical LOC554469...    45   0.001
BC155593_1(BC155593|pid:none) Danio rerio zgc:172065, mRNA (cDNA...    44   0.004
CP001080_932(CP001080|pid:none) Sulfurihydrogenibium sp. YO3AOP1...    44   0.005
BC144320_1(BC144320|pid:none) Homo sapiens coiled-coil domain co...    43   0.006
AB201172_1(AB201172|pid:none) Homo sapiens GRDN mRNA for girdin,...    43   0.006
BC132736_1(BC132736|pid:none) Homo sapiens coiled-coil domain co...    43   0.006
BC078168_1(BC078168|pid:none) Homo sapiens coiled-coil domain co...    43   0.006
(Q3V6T2) RecName: Full=Girdin; AltName: Full=Girders of actin fi...    43   0.006

 psg: 0.76  gvh: 0.10  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for CHS103 is nuc

>CHS103Z.Seq
NNNNNNNNNNGAAAGATTGTTATGCGATGAACAATACATTGTATTGCAAAGAGTGTAAAT
TCAATTGTCATTATTCAAAACATATGCATATCACATATGAAACATTTTTAATTAAAAAGG
AAATGGAAAACGAAGATGTTAAAAAACAAATTGAAGAAGGAATTGATGCAACATCTTTGA
AAAGACAATGGGTTGAATCCTTAGAGAAAGAAATTAAGGAAAATACTGATGAGAAAAATG
AAATACTTAAAATTAGCTCACAACTTTCATACTTTTTAAAAGAAAATTGCATTACAACAT
ACCATGATACAGTCATTGAATATTTGAAAATTTGTATTGAAAATGAAGAACTCAAATCAG
ATAAATCAGGTAAAAAATCAATGGAAGATAATTTAAAGTTTTTCCAAGATCAACATGATT
ATTTTCAAAAAAATCTTTCCAACCAACAAATCCAAAAAATCACAACAAAAGATGTCCCAA
TTCTCATTGAAAAATTAAAAAACCTCAAAGAAAATGGTAAAACACTTAGAGAATGCATCG
AAGCTAATGTAAATGAACAATTAAAAACAGGCTTCAATCCAATTGTTGTCACAAAAAATA
CAAATCATTCAAAGGAATGGGGTTTTATTTC