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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH493
Library AF
(Link to library)
Clone ID AFH493
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U16068-1
Original site URL
Representative seq. ID AFH493P
(Link to Original site)
Representative DNA sequence 
>AFH493 (AFH493Q) /CSM/AF/AFH4-D/AFH493Q.Seq.d/
ATATAACAATCTTCATGAAATTTAATATTAACTTTAACTTTTACAAATAGAATAGATAAC
ATCACCAAAAATAAAAAAAAAATAAAAAAAAAATAAAAAAAANCTCAAAAATAAAATAAT
AATAATAATAATAAAAAAACTTTAAAATGAGTTCAAAGGATCAGATNTTGGAGAAAGATA
AAAAGGAGACAGATGATAATGGCAATAAAAAGACAACAACAACAACTTCATCATCATCAT
CATCATCATCATCATCAAAACCAAGATCAAATAAATTTGATAAAGTAATCATTAAATCAA
ATGGAGTTTGTTATATAACAGAGGGGGTAATTGGAAATGGCTCATTTGGNGTCGTCACTC
AAGCAATTGNGGCTGATACCAAAGAGGTTGTAGCAATAAAAAAGGTTTTACAAGACCAAA
GATNCAAGAATCGAGAATTACAAATCATGAAGATGTTAAATCATATAAATATAGTTTCAT
TAAAAAATAGTTTCTXXXXXXXXXXTTATTGGGTCAACCGGTTATTCCCTGGGTGAGAAT
GGTATTGACCAATTGGTAGAGATTATAAAGGTATTGGGTACACCAACCAAAGAACAAATT
CATGCAATGAATCCATATTATACCTCATTTAAATTCCCAGAGATCAAAGCAAACCCATGG
CCAAGAGTATTCAAAGCTAAGGATGTACCGGCCGAATCAATTGATTTAATCTCAAAAATC
TTACTTTATGATCCATCCTCAAGATTAAAGCCAGTTGAAATTTGTGCACATCCATTCTTT
GATGAATTAAGAGATCCAAAGACATGTCTTCCAGATGGTAAACCTTTACCACCCCTCTTC
AACTTTACAATCGCAGAACAAACTTCAATCGGTCCAAAACTAGCCAAAACATTAATTCCA
TCACATGCTATGAATCAAATTGAATTGCCATCACCTTTATTCCCAAATTTGGCAATCTCT
TCTTCTAATCAATCCTCCTCTTCAAATTCAAATGCAAATGTGTCATCAAATTTAAATTCT
CATTCTGCTTCACCTTCAACTACCTCCTCCTCTTCTTCTACTCCAAATTCAATTCCTGTT
CAATCACCTTCTACAACCAACACTACTTCATCAACAACTAACAACACAACCACAACTACA
ACAACAACAACTACTTCAAATCATTAAAACTATAAACCTCTC
sequence update 2001. 6. 2
Translated Amino Acid sequence 
itifmkfninfnfyk*nr*hhqk*kknkkkikkxskik******KNFKMSSKDQXLEKDK
KETDDNGNKKTTTTTSSSSSSSSSSKPRSNKFDKVIIKSNGVCYITEGVIGNGSFGVVTQ
AIXADTKEVVAIKKVLQDQRXKNRELQIMKMLNHINIVSLKNSF---

---IGSTGYSLGENGIDQLVEIIKVLGTPTKEQIHAMNPYYTSFKFPEIKANPWPRVFKA
KDVPAESIDLISKILLYDPSSRLKPVEICAHPFFDELRDPKTCLPDGKPLPPLFNFTIAE
QTSIGPKLAKTLIPSHAMNQIELPSPLFPNLAISSSNQSSSSNSNANVSSNLNSHSASPS
TTSSSSSTPNSIPVQSPSTTNTTSSTTNNTTTTTTTTTTSNH*nykpl
Translated Amino Acid sequence (All Frames) 
Frame A:
i*qss*nliltltftnridnitknkkkikkk*kkxqk*nnnnnnkktlk*vqrirxwrki
krrqmimaikrqqqqlhhhhhhhhhqnqdqinlik*slnqmefvi*qrg*lemahlxssl
kqlxlipkrl*q*krfyktkdxrienyks*rc*ii*i*fh*kivs---

---llgqpvipwvrmvltnw*rl*rywvhqpknkfmq*ihiiphlnsqrskqthgqeysk
lrmyrpnqli*sqksyfmihpqd*sqlkfvhihslmn*eiqrhvfqmvnlyhpsstlqsq
nklqsvqn*pkh*fhhml*iklnchhlysqiwqslllinpplqiqmqmchqi*ilillhl
qlpppllllqiqflfnhllqptllhqqlttqpqlqqqqllqiiktinl

Frame B:
ynnlhei*y*l*llqie*itspkikkk*kknkkxlknkiiiiiikkl*nefkgsdxger*
kgdr**wq*kdnnnnfiiiiiiiiiktkik*i**snh*ikwsllynrggnwkwliwxrhs
snxg*yqrgcsnkkgftrpkxqesritnhedvksykysfikk*f---

---ywvnrlfpg*ewy*pigrdykgigytnqrtnscnesilyli*iprdqskpmaksiqs
*gctgrin*fnlknltl*silkikas*nlctsil**ikrskdmssrw*tfttplqlynrr
tnfnrsktsqninsitcyesn*iaitfipkfgnlff*sillfkfkckcvikfkfsfcftf
nylllffyskfnscsitfynqhyfinn*qhnhnynnnnyfkslkl*ts

Frame C:
itifmkfninfnfyk*nr*hhqk*kknkkkikkxskik******KNFKMSSKDQXLEKDK
KETDDNGNKKTTTTTSSSSSSSSSSKPRSNKFDKVIIKSNGVCYITEGVIGNGSFGVVTQ
AIXADTKEVVAIKKVLQDQRXKNRELQIMKMLNHINIVSLKNSF---

---IGSTGYSLGENGIDQLVEIIKVLGTPTKEQIHAMNPYYTSFKFPEIKANPWPRVFKA
KDVPAESIDLISKILLYDPSSRLKPVEICAHPFFDELRDPKTCLPDGKPLPPLFNFTIAE
QTSIGPKLAKTLIPSHAMNQIELPSPLFPNLAISSSNQSSSSNSNANVSSNLNSHSASPS
TTSSSSSTPNSIPVQSPSTTNTTSSTTNNTTTTTTTTTTSNH*nykpl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH493 (AFH493Q) /CSM/AF/AFH4-D/AFH493Q.Seq.d/                       1574   0.0
CFG633 (CFG633Q) /CSM/CF/CFG6-B/CFG633Q.Seq.d/                       1128   0.0
CFC364 (CFC364Q) /CSM/CF/CFC3-C/CFC364Q.Seq.d/                       1128   0.0
CFA794 (CFA794Q) /CSM/CF/CFA7-D/CFA794Q.Seq.d/                       1128   0.0
CHA261 (CHA261Q) /CSM/CH/CHA2-C/CHA261Q.Seq.d/                       1122   0.0
SSL104 (SSL104Q) /CSM/SS/SSL1-A/SSL104Q.Seq.d/                       1108   0.0
CHB117 (CHB117Q) /CSM/CH/CHB1-A/CHB117Q.Seq.d/                       1102   0.0
CFC263 (CFC263Q) /CSM/CF/CFC2-C/CFC263Q.Seq.d/                       1098   0.0
SFD633 (SFD633Q) /CSM/SF/SFD6-B/SFD633Q.Seq.d/                       1041   0.0
AFH106 (AFH106Q) /CSM/AF/AFH1-A/AFH106Q.Seq.d/                        870   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L34674|L34674.2 Dictyostelium discoideum glycogen synthase kinase 3 mRNA, complete cds.  1108   0.0    4
AC116920|AC116920.3 Dictyostelium discoideum chromosome 2 map 4415041-4520361 strain AX4, complete sequence.  1108   0.0    10
BJ552082|BJ552082.1 Hordeum vulgare subsp. spontaneum strain H602 cDNA clone:bah47d12, 3' mRNA sequence.    44   3e-07  3
BJ552583|BJ552583.1 Hordeum vulgare subsp. spontaneum strain H602 cDNA clone:bah55o11, 3' mRNA sequence.    44   3e-07  3
AV927751|AV927751.1 Hordeum vulgare subsp. vulgare cv.Haruna Nijo cDNA clone:basd3i06, 3' mRNA sequence.    44   3e-07  3
BD365141|BD365141.1 Newly found single nucleotide polymorphisms (SNPs) on genes and use thereof.    44   3e-07  3
BD365143|BD365143.1 Newly found single nucleotide polymorphisms (SNPs) on genes and use thereof.    44   3e-07  3
BD365137|BD365137.1 Newly found single nucleotide polymorphisms (SNPs) on genes and use thereof.    44   3e-07  3
BJ552379|BJ552379.1 Hordeum vulgare subsp. spontaneum strain H602 cDNA clone:bah56e23, 3' mRNA sequence.    44   3e-07  3
CK261412|CK261412.1 EST707490 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAB972 3' end, mRNA sequence.    48   2e-06  2
dna update 2004. 5.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A55476(A55476)protein kinase (EC 2.7.1.37) gskA - slime mold  (D...   294   4e-78
EF677494_1(EF677494|pid:none) Picea sitchensis clone WS02769_M19...   169   2e-40
AC096856_19(AC096856|pid:none) Oryza sativa chromosome 3 BAC OSJ...   166   1e-39
EF085206_1(EF085206|pid:none) Picea sitchensis clone WS02728_K01...   166   1e-39
T03601(T03601) shaggy protein kinase (EC 2.7.1.-) 6 - common tob...   164   4e-39
AJ224163_1(AJ224163|pid:none) Nicotiana tabacum mRNA for shaggy-...   164   4e-39
AY722707_1(AY722707|pid:none) Zea mays clone ASK1 putative salt-...   163   9e-39
BT068017_1(BT068017|pid:none) Zea mays full-length cDNA clone ZM...   163   1e-38
AY339069_1(AY339069|pid:none) Physcomitrella patens shaggy-relat...   163   1e-38
AB032265_1(AB032265|pid:none) Danio rerio mRNA for glycogen synt...   162   2e-38
protein update 2009. 4.21
PSORT 

 psg: 0.29  gvh: 0.49  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for AFH493 is nuc
5' end seq. ID AFH493F
5' end seq. 
>AFH493F.Seq
ATATAACAATCTTCATGAAATTTAATATTAACTTTAACTTTTACAAATAGAATAGATAAC
ATCACCAAAAATAAAAAAAAAATAAAAAAAAAATAAAAAAAANCTCAAAAATAAAATAAT
AATAATAATAATAAAAAAACTTTAAAATGAGTTCAAAGGATCAGATNTTGGAGAAAGATA
AAAAGGAGACAGATGATAATGGCAATAAAAAGACAACAACAACAACTTCATCATCATCAT
CATCATCATCATCATCAAAACCAAGATCAAATAAATTTGATAAAGTAATCATTAAATCAA
ATGGAGTTTGTTATATAACAGAGGGGGTAATTGGAAATGGCTCATTTGGNGTCGTCACTC
AAGCAATTGNGGCTGATACCAAAGAGGTTGTAGCAATAAAAAAGGTTTTACAAGACCAAA
GATNCAAGAATCGAGAATTACAAATCATGAAGATGTTAAATCATATAAATATAGTTTCAT
TAAAAAATAGTTTCT----------
Length of 5' end seq. 495
3' end seq. ID AFH493Z
3' end seq. 
>AFH493Z.Seq
----------TTATTGGGTCAACCGGTTATTCCCTGGGTGAGAATGGTATTGACCAATTG
GTAGAGATTATAAAGGTATTGGGTACACCAACCAAAGAACAAATTCATGCAATGAATCCA
TATTATACCTCATTTAAATTCCCAGAGATCAAAGCAAACCCATGGCCAAGAGTATTCAAA
GCTAAGGATGTACCGGCCGAATCAATTGATTTAATCTCAAAAATCTTACTTTATGATCCA
TCCTCAAGATTAAAGCCAGTTGAAATTTGTGCACATCCATTCTTTGATGAATTAAGAGAT
CCAAAGACATGTCTTCCAGATGGTAAACCTTTACCACCCCTCTTCAACTTTACAATCGCA
GAACAAACTTCAATCGGTCCAAAACTAGCCAAAACATTAATTCCATCACATGCTATGAAT
CAAATTGAATTGCCATCACCTTTATTCCCAAATTTGGCAATCTCTTCTTCTAATCAATCC
TCCTCTTCAAATTCAAATGCAAATGTGTCATCAAATTTAAATTCTCATTCTGCTTCACCT
TCAACTACCTCCTCCTCTTCTTCTACTCCAAATTCAATTCCTGTTCAATCACCTTCTACA
ACCAACACTACTTCATCAACAACTAACAACACAACCACAACTACAACAACAACAACTACT
TCAAATCATTAAAACTATAAACCTCTC
Length of 3' end seq. 677
Connected seq. ID AFH493P
Connected seq. 
>AFH493P.Seq
ATATAACAATCTTCATGAAATTTAATATTAACTTTAACTTTTACAAATAGAATAGATAAC
ATCACCAAAAATAAAAAAAAAATAAAAAAAAAATAAAAAAAANCTCAAAAATAAAATAAT
AATAATAATAATAAAAAAACTTTAAAATGAGTTCAAAGGATCAGATNTTGGAGAAAGATA
AAAAGGAGACAGATGATAATGGCAATAAAAAGACAACAACAACAACTTCATCATCATCAT
CATCATCATCATCATCAAAACCAAGATCAAATAAATTTGATAAAGTAATCATTAAATCAA
ATGGAGTTTGTTATATAACAGAGGGGGTAATTGGAAATGGCTCATTTGGNGTCGTCACTC
AAGCAATTGNGGCTGATACCAAAGAGGTTGTAGCAATAAAAAAGGTTTTACAAGACCAAA
GATNCAAGAATCGAGAATTACAAATCATGAAGATGTTAAATCATATAAATATAGTTTCAT
TAAAAAATAGTTTCT----------TTATTGGGTCAACCGGTTATTCCCTGGGTGAGAAT
GGTATTGACCAATTGGTAGAGATTATAAAGGTATTGGGTACACCAACCAAAGAACAAATT
CATGCAATGAATCCATATTATACCTCATTTAAATTCCCAGAGATCAAAGCAAACCCATGG
CCAAGAGTATTCAAAGCTAAGGATGTACCGGCCGAATCAATTGATTTAATCTCAAAAATC
TTACTTTATGATCCATCCTCAAGATTAAAGCCAGTTGAAATTTGTGCACATCCATTCTTT
GATGAATTAAGAGATCCAAAGACATGTCTTCCAGATGGTAAACCTTTACCACCCCTCTTC
AACTTTACAATCGCAGAACAAACTTCAATCGGTCCAAAACTAGCCAAAACATTAATTCCA
TCACATGCTATGAATCAAATTGAATTGCCATCACCTTTATTCCCAAATTTGGCAATCTCT
TCTTCTAATCAATCCTCCTCTTCAAATTCAAATGCAAATGTGTCATCAAATTTAAATTCT
CATTCTGCTTCACCTTCAACTACCTCCTCCTCTTCTTCTACTCCAAATTCAATTCCTGTT
CAATCACCTTCTACAACCAACACTACTTCATCAACAACTAACAACACAACCACAACTACA
ACAACAACAACTACTTCAAATCATTAAAACTATAAACCTCTC
Length of connected seq. 1172
Full length Seq ID -
Full length Seq. -
Length of full length seq. -