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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH472
Library AF
(Link to library)
Clone ID AFH472
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U08770-1
Original site URL
Representative seq. ID AFH472P
(Link to Original site)
Representative DNA sequence 
>AFH472 (AFH472Q) /CSM/AF/AFH4-C/AFH472Q.Seq.d/
CCACCATCTTCCATTGAAAAAGGTATACTTCCAAAGGTATTACATGGTTTGGTTTCAAAG
AGAAGAGAAATTAAAAAGAGAATGGAGCAAGAAAAGAATAAAATAATAAAAGCACAATAT
GATATTCAACAACAAGCCGTTAAGTTAATTGCAAATTCAATGTATGGTTGCTTAGGTTTC
TCTCATAGTAGATTCTATGCATTACCATTGGCAGAGTTGGTCACTAGAAAGGGTAGAGAA
AATCTTCAAAAGGGTGCTTCCATCGTTAATAAAATGTGCTATGATGTAATCTATGGTGAT
ACTGATTCCCTTATGATCTACACTGGTGTCGGTACTTTCAATGAAGCTGAAACCATTGGT
AAAGAAATTCAAAAGAAAATCAATGACCAATATCGTGGTAGTGTAATGGAAATTGGTTTA
GATGGTATTTTCAAACGTTTATTATTATTTAAAAAGAAGAAATATGCTTGTTTGAAGGAA
TTCCGTATCGATTCAACCACTACCAAATGTGAGAGAGAAAATAAAGGTATCGATATTGTT
CGTAGAGATTATTGTGACCTCACTAAGXXXXXXXXXXTTCGATTGCAACCAATGTCATGC
TAAAATCCCATTAAAGAAAATTAGCTAATCAATTNCAATTAAACATTAGAACTTATTTGA
AGCAATATAATGATTGGGATTTACGATGCACCGAATGTGAAAAAGTCTNTAAAAACTATA
AAGAAACCTNTTATCGTTGCGCTAGACCTCAATGTCGTGGTAAAATGATTCAAATTATGA
CAAGTTCAAAATTGTTTAATCAAATTAGTTTCTTTTCAAAACTATTTANAAATGATCTTT
CAAACTCTGATAATACCACTACAATCATTCCAAATGAAGATCAAAATACTCTTAAACAAG
CAAAACAAATAATTGATTCTTTTCTCTCTAAATTTGATCAATATAATGTAAATTTAAATT
CACTCTTAACTCCTTCNCAAACNTTAGATAGTTTTAATAATTATTTAGCTTCNTCNCGAG
CTAGNATTCAATATCCAATACTTAATAAATAAAATTTTAAAAAAAATAAATAAAATAAAA
TAAAAATAAAGAATTCTAATTTTAAAATCAAATTAAAAATAAATTATTTTAAAATCNCGG
AAAATTTATTCCNTTNAAAAAAATCCAAGAAAAAAAAAATAAAATAAATAATAAAAATAA
AAAAAAAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
PPSSIEKGILPKVLHGLVSKRREIKKRMEQEKNKIIKAQYDIQQQAVKLIANSMYGCLGF
SHSRFYALPLAELVTRKGRENLQKGASIVNKMCYDVIYGDTDSLMIYTGVGTFNEAETIG
KEIQKKINDQYRGSVMEIGLDGIFKRLLLFKKKKYACLKEFRIDSTTTKCERENKGIDIV
RRDYCDLTK---

---FDCNQCHAKIPLKKIS*sixikh*nlfeai**lgftmhrm*ksl*kl*rnllslr*t
smsw*ndsnydkfkiv*sn*flfktixk*sfkl**yhynhsk*rskys*tsktnn*ffsl
*i*si*ckfkftlnsfxnxr*f**lfsfxxs*xsisnt**ikf*kk*ik*nknkef*f*n
qiknklf*nxgkfipxkkiqekknkinnknkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
PPSSIEKGILPKVLHGLVSKRREIKKRMEQEKNKIIKAQYDIQQQAVKLIANSMYGCLGF
SHSRFYALPLAELVTRKGRENLQKGASIVNKMCYDVIYGDTDSLMIYTGVGTFNEAETIG
KEIQKKINDQYRGSVMEIGLDGIFKRLLLFKKKKYACLKEFRIDSTTTKCERENKGIDIV
RRDYCDLTK---

---FDCNQCHAKIPLKKIS*sixikh*nlfeai**lgftmhrm*ksl*kl*rnllslr*t
smsw*ndsnydkfkiv*sn*flfktixk*sfkl**yhynhsk*rskys*tsktnn*ffsl
*i*si*ckfkftlnsfxnxr*f**lfsfxxs*xsisnt**ikf*kk*ik*nknkef*f*n
qiknklf*nxgkfipxkkiqekknkinnknkkk

Frame B:
hhlplkkvyfqryymvwfqreeklkrewskkrik**khnmifnnkpls*lqiqcmva*vs
livdsmhyhwqswslervekifkrvlpslikcamm*smvilipl*stlvsvlsmklkplv
kkfkrksmtnivvv*wklv*mvfsnvyyylkrrnmlv*rnsvsiqplpnvrekikvsilf
veiivtsl---

---siatnvmlksh*rklanqxqlnirtylkqyndwdlrctecekvxknyketxyrcarp
qcrgkmiqimtssklfnqisffsklfxndlsnsdntttiipnedqntlkqakqiidsfls
kfdqynvnlnslltpsqtldsfnnylassraxiqypilnk*nfkknk*nkikiknsnfki
klkinyfkixenlfxxkkskkkkik*iikikkn

Frame C:
tifh*krytskgitwfgfkekrn*kengarke*nnksti*ysttsr*vnckfnvwllrfl
s**ilcitigrvgh*kg*rksskgcfhr**nvl*cnlw*y*fpydlhwcryfq*s*nhw*
rnskenq*pisw*cngnwfrwyfqtfiii*keeiclfegipyrfnhyqm*erk*ryrycs
*rll*ph*---

---rlqpmsc*npiken*linxn*tleli*snimigiydapnvkksxktikkpxivaldl
nvvvk*fkl*qvqncliklvsfqnylxmifqtliiplqsfqmkikillnkqnk*lilfsl
nlinim*i*ihs*llxkx*ivliii*lxxelxfniqylinkilkkinkik*k*rililks
n*k*iilksrkiysxxknprkkk*nk**k*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH472 (AFH472Q) /CSM/AF/AFH4-C/AFH472Q.Seq.d/                       1953   0.0
VSE828 (VSE828Q) /CSM/VS/VSE8-B/VSE828Q.Seq.d/                         42   0.020
SSB324 (SSB324Q) /CSM/SS/SSB3-A/SSB324Q.Seq.d/                         42   0.020
AFE215 (AFE215Q) /CSM/AF/AFE2-A/AFE215Q.Seq.d/                         40   0.077
SSA723 (SSA723Q) /CSM/SS/SSA7-A/SSA723Q.Seq.d/                         38   0.30
SHD749 (SHD749Q) /CSM/SH/SHD7-C/SHD749Q.Seq.d/                         38   0.30
SHA625 (SHA625Q) /CSM/SH/SHA6-B/SHA625Q.Seq.d/                         38   0.30
VSJ622 (VSJ622Q) /CSM/VS/VSJ6-A/VSJ622Q.Seq.d/                         36   1.2
VSI637 (VSI637Q) /CSM/VS/VSI6-B/VSI637Q.Seq.d/                         36   1.2
VSH792 (VSH792Q) /CSM/VS/VSH7-D/VSH792Q.Seq.d/                         36   1.2
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    36   1e-06  13
AE014839|AE014839.1 Plasmodium falciparum 3D7 chromosome 11 section 4 of 8 of the complete sequence.    36   5e-06  13
AE014821|AE014821.1 Plasmodium falciparum 3D7 chromosome 14 section 6 of 13 of the complete sequence.    32   8e-05  17
AC116919|AC116919.2 Dictyostelium discoideum chromosome 2 map complement(6245035-6206343) strain AX4, complete sequence.    40   3e-04  8
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34   3e-04  20
AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    36   7e-04  15
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    32   0.001  13
AC090532|AC090532.1 Homo sapiens chromosome 15 clone RP11-325L11 map 15q21.3, complete sequence.    38   0.001  8
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    36   0.001  15
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    32   0.002  17
dna update 2004. 5.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54SV8) RecName: Full=DNA polymerase alpha catalytic subunit;  ...   354   4e-96
AB178528_1(AB178528|pid:none) Caiman crocodilus mRNA for DNA pol...   164   4e-39
AB178527_1(AB178527|pid:none) Iguana iguana mRNA for DNA polymer...   164   4e-39
AB178529_1(AB178529|pid:none) Gallus gallus mRNA for DNA polymer...   163   9e-39
AB178526_1(AB178526|pid:none) Trachemys scripta mRNA for DNA pol...   163   1e-38
(P09884) RecName: Full=DNA polymerase alpha catalytic subunit;  ...   160   1e-37
DJHUAC(S00257;A30440;I57513;S12665) DNA-directed DNA polymerase ...   160   1e-37
AY648733_1(AY648733|pid:none) Danio rerio DNA polymerase alpha m...   159   1e-37
(Q9DE46) RecName: Full=DNA polymerase alpha catalytic subunit;  ...   159   2e-37
BC161721_1(BC161721|pid:none) Xenopus laevis DNA polymerase alph...   159   2e-37
protein update 2009. 4.21
PSORT 

 psg: 0.76  gvh: 0.46  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.10
 nuc: 0.04  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 28.0 %: mitochondrial

>> prediction for AFH472 is nuc
5' end seq. ID AFH472F
5' end seq. 
>AFH472F.Seq
CCACCATCTTCCATTGAAAAAGGTATACTTCCAAAGGTATTACATGGTTTGGTTTCAAAG
AGAAGAGAAATTAAAAAGAGAATGGAGCAAGAAAAGAATAAAATAATAAAAGCACAATAT
GATATTCAACAACAAGCCGTTAAGTTAATTGCAAATTCAATGTATGGTTGCTTAGGTTTC
TCTCATAGTAGATTCTATGCATTACCATTGGCAGAGTTGGTCACTAGAAAGGGTAGAGAA
AATCTTCAAAAGGGTGCTTCCATCGTTAATAAAATGTGCTATGATGTAATCTATGGTGAT
ACTGATTCCCTTATGATCTACACTGGTGTCGGTACTTTCAATGAAGCTGAAACCATTGGT
AAAGAAATTCAAAAGAAAATCAATGACCAATATCGTGGTAGTGTAATGGAAATTGGTTTA
GATGGTATTTTCAAACGTTTATTATTATTTAAAAAGAAGAAATATGCTTGTTTGAAGGAA
TTCCGTATCGATTCAACCACTACCAAATGTGAGAGAGAAAATAAAGGTATCGATATTGTT
CGTAGAGATTATTGTGACCTCACTAAG----------
Length of 5' end seq. 567
3' end seq. ID AFH472Z
3' end seq. 
>AFH472Z.Seq
----------TTCGATTGCAACCAATGTCATGCTAAAATCCCATTAAAGAAAATTAGCTA
ATCAATTNCAATTAAACATTAGAACTTATTTGAAGCAATATAATGATTGGGATTTACGAT
GCACCGAATGTGAAAAAGTCTNTAAAAACTATAAAGAAACCTNTTATCGTTGCGCTAGAC
CTCAATGTCGTGGTAAAATGATTCAAATTATGACAAGTTCAAAATTGTTTAATCAAATTA
GTTTCTTTTCAAAACTATTTANAAATGATCTTTCAAACTCTGATAATACCACTACAATCA
TTCCAAATGAAGATCAAAATACTCTTAAACAAGCAAAACAAATAATTGATTCTTTTCTCT
CTAAATTTGATCAATATAATGTAAATTTAAATTCACTCTTAACTCCTTCNCAAACNTTAG
ATAGTTTTAATAATTATTTAGCTTCNTCNCGAGCTAGNATTCAATATCCAATACTTAATA
AATAAAATTTTAAAAAAAATAAATAAAATAAAATAAAAATAAAGAATTCTAATTTTAAAA
TCAAATTAAAAATAAATTATTTTAAAATCNCGGAAAATTTATTCCNTTNAAAAAAATCCA
AGAAAAAAAAAATAAAATAAATAATAAAAATAAAAAAAAAT
Length of 3' end seq. 631
Connected seq. ID AFH472P
Connected seq. 
>AFH472P.Seq
CCACCATCTTCCATTGAAAAAGGTATACTTCCAAAGGTATTACATGGTTTGGTTTCAAAG
AGAAGAGAAATTAAAAAGAGAATGGAGCAAGAAAAGAATAAAATAATAAAAGCACAATAT
GATATTCAACAACAAGCCGTTAAGTTAATTGCAAATTCAATGTATGGTTGCTTAGGTTTC
TCTCATAGTAGATTCTATGCATTACCATTGGCAGAGTTGGTCACTAGAAAGGGTAGAGAA
AATCTTCAAAAGGGTGCTTCCATCGTTAATAAAATGTGCTATGATGTAATCTATGGTGAT
ACTGATTCCCTTATGATCTACACTGGTGTCGGTACTTTCAATGAAGCTGAAACCATTGGT
AAAGAAATTCAAAAGAAAATCAATGACCAATATCGTGGTAGTGTAATGGAAATTGGTTTA
GATGGTATTTTCAAACGTTTATTATTATTTAAAAAGAAGAAATATGCTTGTTTGAAGGAA
TTCCGTATCGATTCAACCACTACCAAATGTGAGAGAGAAAATAAAGGTATCGATATTGTT
CGTAGAGATTATTGTGACCTCACTAAG----------TTCGATTGCAACCAATGTCATGC
TAAAATCCCATTAAAGAAAATTAGCTAATCAATTNCAATTAAACATTAGAACTTATTTGA
AGCAATATAATGATTGGGATTTACGATGCACCGAATGTGAAAAAGTCTNTAAAAACTATA
AAGAAACCTNTTATCGTTGCGCTAGACCTCAATGTCGTGGTAAAATGATTCAAATTATGA
CAAGTTCAAAATTGTTTAATCAAATTAGTTTCTTTTCAAAACTATTTANAAATGATCTTT
CAAACTCTGATAATACCACTACAATCATTCCAAATGAAGATCAAAATACTCTTAAACAAG
CAAAACAAATAATTGATTCTTTTCTCTCTAAATTTGATCAATATAATGTAAATTTAAATT
CACTCTTAACTCCTTCNCAAACNTTAGATAGTTTTAATAATTATTTAGCTTCNTCNCGAG
CTAGNATTCAATATCCAATACTTAATAAATAAAATTTTAAAAAAAATAAATAAAATAAAA
TAAAAATAAAGAATTCTAATTTTAAAATCAAATTAAAAATAAATTATTTTAAAATCNCGG
AAAATTTATTCCNTTNAAAAAAATCCAAGAAAAAAAAAATAAAATAAATAATAAAAATAA
AAAAAAAT
Length of connected seq. 1198
Full length Seq ID -
Full length Seq. -
Length of full length seq. -