>CHR591 (CHR591Q) /CSM/CH/CHR5-D/CHR591Q.Seq.d/
ATTCATAGATNACATCCACTTTCTCAATTCAATCACCGTCTCGTGGTGATAGGGTTTTAA
ATAGAGCTGAAAGTAATTTTATAACAAGTGTAAATAGTAGTAATAATAAACAAACTTCTC
AACCATTAAGAGATGATATAAAATCAAACAGTAGTGATAAACTATTACAACAACCAGATA
ATATTGATCCATATGAAATTCAACAAAATAAACAATCCAATAATAGCAATAGCAATAGCA
ATAGAAATTTAACACCAAATTCATCATCACCAACAAATCAAAGAAAAAATAAACAAGAAG
ATGATGATGATGAATCTAAATTAGATGATGAATCAGATTTGAATGAAAGATTTCAAAAAG
TTGTACTTAGATTTAGAGAATTTCCATCATTGGATACAAATCTTTATCGCTTACAAGAGA
TTTGTACAGATTTATTACATATTTCTCAAGATTTTATACATACTGTCAAAACCTATGGTA
GAATTATCATTGAAGAACGTTATTTAAAAGAGAAAACCATTCAAAGTCGTTCAATTGGTG
GTCATTTXXXXXXXXXXTTAGAAAGAAACCCATTGAAAAAGTGGCCGATACTCAATCAAA
TCTTTGGAATCATTATCAACCATTCGATACTAGTGATTTGAAAAAGATTGGTGTACGTGT
TAAACATACTGATTTGGTTACAAATGCTGAAGGTACCTTCTTTTATGCTAAAGCAATGAC
TGAACGTTCAATTGGTACCGCCATTCATTTATTACATAAAGCTAGAAAATTCTTTGAATT
GGCATTAATTTCAAATCCAAACAATAAGGAAACCTTACAACTATGTGCTCAAGCTTGGTG
TAAAATTTTGGAATTTAGTGCAAGTCAAGGTAAAAATATGTCCAATGTTAGATTTTCAAT
GTCTGATCGTGTCGTGGTAAATACTGATCGTTATTTCTTACGTGCTATCGATGCCGATAC
TAAAGGTCCCCTCATTCTCTTCTTTTACGCAAACTTTTTAGTTCGTTGTGAACGTTATGA
AAAAGCTGAAGATTATTTCCTTCGTTCTCTTGAAGCTGATTCAAATAATTATCGTTGTTT
AACCGCTTATGCAAACTTTTTAATTGAAAGAGGTTTTCCAAATGAATCTTCTCTTTTCTT
AGAAAGAGCAAAACAATGTAAAGCAATCTTAGTTGGTGGTTTTAAAAATTAAATTTTTAA
AAAAATTTAACATATATATAATATTAAATAAATAAATTAAATTAAATAAATAA

s*XTSTFSIQSPSRGDRVLNRAESNFITSVNSSNNKQTSQPLRDDIKSNSSDKLLQQPDN
IDPYEIQQNKQSNNSNSNSNRNLTPNSSSPTNQRKNKQEDDDDESKLDDESDLNERFQKV
VLRFREFPSLDTNLYRLQEICTDLLHISQDFIHTVKTYGRIIIEERYLKEKTIQSRSIGG
H---

---RKKPIEKVADTQSNLWNHYQPFDTSDLKKIGVRVKHTDLVTNAEGTFFYAKAMTERS
IGTAIHLLHKARKFFELALISNPNNKETLQLCAQAWCKILEFSASQGKNMSNVRFSMSDR
VVVNTDRYFLRAIDADTKGPLILFFYANFLVRCERYEKAEDYFLRSLEADSNNYRCLTAY
ANFLIERGFPNESSLFLERAKQCKAILVGGFKN*ifkki*hiynik*in*ik*i

Frame A:
ihrxhplsqfnhrlvvigf*ielkvil*qv*ivviinkllnh*emi*nqtvvinyynnqi
ilihmkfnkinnpiiaiaiaiei*hqihhhqqikekinkkmmmmnln*mmnqi*mkdfkk
lyldlenfhhwiqifiaykrfvqiyyiflkilyilskpmvelslknvi*krkpfkvvqlv
vi---

---lernplkkwpilnqifgiiinhsilvi*krlvyvlniliwlqmlkvpsfmlkq*lnv
qlvppfiyyiklenslnwh*fqiqtirkpynyvlklgvkfwnlvqvkvkicpmldfqcli
vsw*ilivisyvlsmpilkvpsfssftqtf*fvvnvmkklkiisfvllkliqiiivv*pl
mqtf*lkevfqmnllfs*keqnnvkqs*lvvlkikflkkfniyiilnk*iklnk*

Frame B:
fidxihflnsitvsw**gfk*s*k*fynkck*****tnfstikr*ykikq***titttr*
y*si*nstk*tiq**q*q*q*kfntkfiitnkskkk*trr****i*ir**irfe*kisks
ct*i*risiigykslsltrdlyrfityfsrfytycqnlw*nyh*rtlfkrenhsksfnww
sf---

---*keth*ksgrysiksleslstiry**fekdwctc*ty*fgykc*rylllc*snd*tf
nwyrhsfit*s*kil*iginfkskq*gnlttmcsslv*nfgi*cksr*kyvqc*ifnv*s
crgky*slfltcyrcry*rsphslllrklfssl*tl*ks*rlfpsfs*s*fk*lslfnrl
cklfn*krfsk*ifsflrksktm*snlswwf*klnf*knltyi*y*inkln*in

Frame C:
s*XTSTFSIQSPSRGDRVLNRAESNFITSVNSSNNKQTSQPLRDDIKSNSSDKLLQQPDN
IDPYEIQQNKQSNNSNSNSNRNLTPNSSSPTNQRKNKQEDDDDESKLDDESDLNERFQKV
VLRFREFPSLDTNLYRLQEICTDLLHISQDFIHTVKTYGRIIIEERYLKEKTIQSRSIGG
H---

---RKKPIEKVADTQSNLWNHYQPFDTSDLKKIGVRVKHTDLVTNAEGTFFYAKAMTERS
IGTAIHLLHKARKFFELALISNPNNKETLQLCAQAWCKILEFSASQGKNMSNVRFSMSDR
VVVNTDRYFLRAIDADTKGPLILFFYANFLVRCERYEKAEDYFLRSLEADSNNYRCLTAY
ANFLIERGFPNESSLFLERAKQCKAILVGGFKN*ifkki*hiynik*in*ik*i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHR591 (CHR591Q) /CSM/CH/CHR5-D/CHR591Q.Seq.d/                       2282   0.0
SHD552 (SHD552Q) /CSM/SH/SHD5-C/SHD552Q.Seq.d/                       1219   0.0
SHF181 (SHF181Q) /CSM/SH/SHF1-D/SHF181Q.Seq.d/                       1207   0.0
CHN252 (CHN252Q) /CSM/CH/CHN2-C/CHN252Q.Seq.d/                       1170   0.0
SHH870 (SHH870Q) /CSM/SH/SHH8-C/SHH870Q.Seq.d/                        967   0.0
VSC329 (VSC329Q) /CSM/VS/VSC3-B/VSC329Q.Seq.d/                         54   5e-06
CFI409 (CFI409Q) /CSM/CF/CFI4-A/CFI409Q.Seq.d/                         50   8e-05
AHF808 (AHF808Q) /CSM/AH/AHF8-A/AHF808Q.Seq.d/                         48   3e-04
VSC328 (VSC328Q) /CSM/VS/VSC3-B/VSC328Q.Seq.d/                         46   0.001
SSD825 (SSD825Q) /CSM/SS/SSD8-B/SSD825Q.Seq.d/                         44   0.005

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY220914|AY220914.1 Dictyostelium discoideum ComB (comB) gene, complete cds.  1227   0.0    1
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    46   4e-05  15
AC116918|AC116918.3 Dictyostelium discoideum chromosome 2 map 6143698-6163521 strain AX4, complete sequence.    50   8e-05  3
CW344417|CW344417.1 104_847_11487943_148_36198_030 Sorghum methylation filtered library (LibID: 104) Sorghum bicolor genomic clone 11487943, genomic survey sequence.    48   0.002  2
CW344416|CW344416.1 104_847_11487943_116_36197_030 Sorghum methylation filtered library (LibID: 104) Sorghum bicolor genomic clone 11487943, genomic survey sequence.    48   0.002  2
AC099163|AC099163.5 Rattus norvegicus clone CH230-50K14, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    54   0.007  1
AC096609|AC096609.7 Rattus norvegicus clone CH230-81I9, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    54   0.007  1
AC094390|AC094390.8 Rattus norvegicus clone CH230-3N16, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    54   0.007  1
AC015522|AC015522.6 Homo sapiens chromosome 8, clone RP11-327O12, complete sequence.    54   0.007  1
AC104940|AC104940.2 Homo sapiens chromosome 8, clone RP11-378J9, complete sequence.    54   0.007  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY220914_1(AY220914|pid:none) Dictyostelium discoideum ComB (com...   419   e-115
AC116963_55(AC116963|pid:none) Dictyostelium discoideum chromoso...    83   2e-14
AP009380_1340(AP009380|pid:none) Porphyromonas gingivalis ATCC 3...    50   1e-04
CP000386_3087(CP000386|pid:none) Rubrobacter xylanophilus DSM 99...    43   0.025
BT052510_1(BT052510|pid:none) Medicago truncatula clone MTYFD_FE...    42   0.057
CP000099_2445(CP000099|pid:none) Methanosarcina barkeri str. Fus...    41   0.096
CP000471_3263(CP000471|pid:none) Magnetococcus sp. MC-1, complet...    40   0.16
BT070955_1(BT070955|pid:none) Picea sitchensis clone WS02761_M06...    39   0.28
EF147030_1(EF147030|pid:none) Populus trichocarpa clone WS01226_...    39   0.37
CP000099_2442(CP000099|pid:none) Methanosarcina barkeri str. Fus...    39   0.37

 psg: 0.44  gvh: 0.28  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for CHR591 is cyt

>CHR591F.Seq
ATTCATAGATNACATCCACTTTCTCAATTCAATCACCGTCTCGTGGTGATAGGGTTTTAA
ATAGAGCTGAAAGTAATTTTATAACAAGTGTAAATAGTAGTAATAATAAACAAACTTCTC
AACCATTAAGAGATGATATAAAATCAAACAGTAGTGATAAACTATTACAACAACCAGATA
ATATTGATCCATATGAAATTCAACAAAATAAACAATCCAATAATAGCAATAGCAATAGCA
ATAGAAATTTAACACCAAATTCATCATCACCAACAAATCAAAGAAAAAATAAACAAGAAG
ATGATGATGATGAATCTAAATTAGATGATGAATCAGATTTGAATGAAAGATTTCAAAAAG
TTGTACTTAGATTTAGAGAATTTCCATCATTGGATACAAATCTTTATCGCTTACAAGAGA
TTTGTACAGATTTATTACATATTTCTCAAGATTTTATACATACTGTCAAAACCTATGGTA
GAATTATCATTGAAGAACGTTATTTAAAAGAGAAAACCATTCAAAGTCGTTCAATTGGTG
GTCATTTNNNNNNNNNN

>CHR591Z.Seq
NNNNNNNNNNTTAGAAAGAAACCCATTGAAAAAGTGGCCGATACTCAATCAAATCTTTGG
AATCATTATCAACCATTCGATACTAGTGATTTGAAAAAGATTGGTGTACGTGTTAAACAT
ACTGATTTGGTTACAAATGCTGAAGGTACCTTCTTTTATGCTAAAGCAATGACTGAACGT
TCAATTGGTACCGCCATTCATTTATTACATAAAGCTAGAAAATTCTTTGAATTGGCATTA
ATTTCAAATCCAAACAATAAGGAAACCTTACAACTATGTGCTCAAGCTTGGTGTAAAATT
TTGGAATTTAGTGCAAGTCAAGGTAAAAATATGTCCAATGTTAGATTTTCAATGTCTGAT
CGTGTCGTGGTAAATACTGATCGTTATTTCTTACGTGCTATCGATGCCGATACTAAAGGT
CCCCTCATTCTCTTCTTTTACGCAAACTTTTTAGTTCGTTGTGAACGTTATGAAAAAGCT
GAAGATTATTTCCTTCGTTCTCTTGAAGCTGATTCAAATAATTATCGTTGTTTAACCGCT
TATGCAAACTTTTTAATTGAAAGAGGTTTTCCAAATGAATCTTCTCTTTTCTTAGAAAGA
GCAAAACAATGTAAAGCAATCTTAGTTGGTGGTTTTAAAAATTAAATTTTTAAAAAAATT
TAACATATATATAATATTAAATAAATAAATTAAATTAAATAAATAA

>CHR591P.Seq
ATTCATAGATNACATCCACTTTCTCAATTCAATCACCGTCTCGTGGTGATAGGGTTTTAA
ATAGAGCTGAAAGTAATTTTATAACAAGTGTAAATAGTAGTAATAATAAACAAACTTCTC
AACCATTAAGAGATGATATAAAATCAAACAGTAGTGATAAACTATTACAACAACCAGATA
ATATTGATCCATATGAAATTCAACAAAATAAACAATCCAATAATAGCAATAGCAATAGCA
ATAGAAATTTAACACCAAATTCATCATCACCAACAAATCAAAGAAAAAATAAACAAGAAG
ATGATGATGATGAATCTAAATTAGATGATGAATCAGATTTGAATGAAAGATTTCAAAAAG
TTGTACTTAGATTTAGAGAATTTCCATCATTGGATACAAATCTTTATCGCTTACAAGAGA
TTTGTACAGATTTATTACATATTTCTCAAGATTTTATACATACTGTCAAAACCTATGGTA
GAATTATCATTGAAGAACGTTATTTAAAAGAGAAAACCATTCAAAGTCGTTCAATTGGTG
GTCATTT----------TTAGAAAGAAACCCATTGAAAAAGTGGCCGATACTCAATCAAA
TCTTTGGAATCATTATCAACCATTCGATACTAGTGATTTGAAAAAGATTGGTGTACGTGT
TAAACATACTGATTTGGTTACAAATGCTGAAGGTACCTTCTTTTATGCTAAAGCAATGAC
TGAACGTTCAATTGGTACCGCCATTCATTTATTACATAAAGCTAGAAAATTCTTTGAATT
GGCATTAATTTCAAATCCAAACAATAAGGAAACCTTACAACTATGTGCTCAAGCTTGGTG
TAAAATTTTGGAATTTAGTGCAAGTCAAGGTAAAAATATGTCCAATGTTAGATTTTCAAT
GTCTGATCGTGTCGTGGTAAATACTGATCGTTATTTCTTACGTGCTATCGATGCCGATAC
TAAAGGTCCCCTCATTCTCTTCTTTTACGCAAACTTTTTAGTTCGTTGTGAACGTTATGA
AAAAGCTGAAGATTATTTCCTTCGTTCTCTTGAAGCTGATTCAAATAATTATCGTTGTTT
AACCGCTTATGCAAACTTTTTAATTGAAAGAGGTTTTCCAAATGAATCTTCTCTTTTCTT
AGAAAGAGCAAAACAATGTAAAGCAATCTTAGTTGGTGGTTTTAAAAATTAAATTTTTAA
AAAAATTTAACATATATATAATATTAAATAAATAAATTAAATTAAATAAATAA