>CHR512 (CHR512Q) /CSM/CH/CHR5-A/CHR512Q.Seq.d/
GTAGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAA
TTATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTA
AATATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACAC
TTAACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAA
CTTTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAGAGATGGG
AAGTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAA
TTGAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTT
TATTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATT
ATTTANAATTAAGTACATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGGAGXXX
XXXXXXXGGTGATCCAGGTGTTAGACCATTATTCTTTGAATATCCATCAGATCCAAATAC
TTTTGCAATTGATCAACAATTTTTAGTTGGTACAGGTTTAATGGTATCACCAGTTCTCAC
TCAAGGTGCTACCACAGTGAATGCTTACTTCCCAAATGATATCTGGTATGAATATGGTAA
TGGTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCTCCATTCGATGTAAT
CAACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACCTCCTCATATGTTAC
ACCAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTACCATTTGAATTGAT
TATTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATCCTTGGATGATGGTGAATC
AATTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGATGTTGTCTCCTCACC
ATCTTCATCTGCCTACAAATTACAATCAACCATTCTCAATAACAATTATAATGGTACCGC
TTCTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTTCAACAAGTCATTGT
TAATGGTAGCCCAATCAATTCATTTAATGCTGTTTCNGATTCAACTCTCTCTGTTTCAAA
TTTACAACTTGCTTTAGATGAATCCTTGAAGTNATTTGTATTATATAAAAAT

svlki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATL
NLISAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYII
EFTNNPFGFAATRISTGEVLFNTTQPSDCSFNGLIYSNYYLXLSTSFTESNPNIYGLG--
-

---GDPGVRPLFFEYPSDPNTFAIDQQFLVGTGLMVSPVLTQGATTVNAYFPNDIWYEYG
NGSLVQSVGTHQTLNAPFDVINVHMRGGNIIPTQPTSSYVTPVDGIPITTKISRTLPFEL
IIALDSSLQATGQLSLDDGESIQTYVDNKYSFIQFDVVSSPSSSAYKLQSTILNNNYNGT
ASLIINSIQIYGSPSVQQVIVNGSPINSFNAVSDSTLSVSNLQLALDESLKXFVLYKN

Frame A:
vvc*kyklklkkk*nfclh*lfflllyyfxlldhyqhhyvniqviqlkxlqkqimvmrqh
ltlfqqvhmvtixnn*ifn*llklvkflxlxlltqiirdgkshqllin*lxkixiqlii*
lnlqiihlvlqqlvfqlxkfyliqlnqvivhlmv*fiqiiixn*vhhsqrviqifmv*e-
--

---GDPGVRPLFFEYPSDPNTFAIDQQFLVGTGLMVSPVLTQGATTVNAYFPNDIWYEYG
NGSLVQSVGTHQTLNAPFDVINVHMRGGNIIPTQPTSSYVTPVDGIPITTKISRTLPFEL
IIALDSSLQATGQLSLDDGESIQTYVDNKYSFIQFDVVSSPSSSAYKLQSTILNNNYNGT
ASLIINSIQIYGSPSVQQVIVNGSPINSFNAVSDSTLSVSNLQLALDESLKXFVLYKN

Frame B:
*cvknin*n*kknetfvfinyffcyciilxcwiiisiim*isrlfnsrxyknk*wl*gnt
*pyfsrsiw*ryxtikfsinf*n*snfxsxny*pk*semgsptnc*siswxksxfn*lyn
*iyk*siwfcsnsyfnwxsfi*ynstk*lfi*wfnlfkllfxikyiihre*skylwfr--
-

---viqvldhyslnihqiqillqlinnf*lvqv*wyhqfslkvlpq*mltsqmisgmnmv
mvhwfnqlvpikl*mlhsm*stficvvvisfqlnqpphmlhqlmvfqlplkslelyhln*
llpwilhykqlvnypwmmvnqfkpmliintlsfnsmlsphhlhlptnynqpfsitiimvp
ll*slilskstvrhqfnksllmvaqsihlmlfxiqlslfqiynll*mnp*sxlyyik

Frame C:
svlki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATL
NLISAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYII
EFTNNPFGFAATRISTGEVLFNTTQPSDCSFNGLIYSNYYLXLSTSFTESNPNIYGLG--
-

---*src*tiil*isirskyfcn*stifswyrfngitsshsrcyhsecllpk*ylv*iw*
wfigsiswypsnfkcsircnqrsyaww*yhsnstnllicyts*wysnyh*nl*nfti*id
yclgffitsnwsiilg*w*insnlc***ilfhsircclltificlqitinhsq*ql*wyr
ffnh*fypnlrfaisstshc*w*pnqfi*ccfxfnslcfkfttcfr*ilevicii*k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHR512 (CHR512Q) /CSM/CH/CHR5-A/CHR512Q.Seq.d/                       2250   0.0
CHR857 (CHR857Q) /CSM/CH/CHR8-C/CHR857Q.Seq.d/                       1340   0.0
AHN661 (AHN661Q) /CSM/AH/AHN6-C/AHN661Q.Seq.d/                       1340   0.0
CHM276 (CHM276Q) /CSM/CH/CHM2-D/CHM276Q.Seq.d/                       1334   0.0
AHM502 (AHM502Q) /CSM/AH/AHM5-A/AHM502Q.Seq.d/                       1334   0.0
AHA841 (AHA841Q) /CSM/AH/AHA8-B/AHA841Q.Seq.d/                       1334   0.0
AHA237 (AHA237Q) /CSM/AH/AHA2-B/AHA237Q.Seq.d/                       1334   0.0
SHG509 (SHG509Q) /CSM/SH/SHG5-A/SHG509Q.Seq.d/                       1332   0.0
CHS485 (CHS485Q) /CSM/CH/CHS4-D/CHS485Q.Seq.d/                       1328   0.0
CHR858 (CHR858Q) /CSM/CH/CHR8-C/CHR858Q.Seq.d/                       1326   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    34   0.22   10
Z70754|Z70754.2 Caenorhabditis elegans Cosmid F58E6.               42   0.61   4
BH944911|BH944911.1 obu81h08.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    46   1.8    1
AC116032|AC116032.2 Dictyostelium discoideum chromosome 2 map 4158743-4189373 strain AX4, complete sequence.    36   2.1    4
AP005808|AP005808.3 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, BAC clone:OSJNBb0038F20.    34   2.3    7
AE014839|AE014839.1 Plasmodium falciparum 3D7 chromosome 11 section 4 of 8 of the complete sequence.    36   2.7    11
AL449924|AL449924.1 Streptococcus pneumoniae serotype 19F  *** SEQUENCING IN PROGRESS  *** from clone G54.    36   2.9    4
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    40   2.9    9
AE014822|AE014822.1 Plasmodium falciparum 3D7 chromosome 14 section 7 of 13 of the complete sequence.    32   3.2    2
AZ690043|AZ690043.1 ENTMH81TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36   4.3    3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF118226_1(AF118226|pid:none) Hordeum vulgare high pI alpha-gluc...   139   2e-31
AB000967_1(AB000967|pid:none) Coturnix japonica GAAI mRNA for ac...   139   2e-31
(Q43763) RecName: Full=Alpha-glucosidase;          EC=3.2.1.20; ...   135   2e-30
AF016833_1(AF016833|pid:none) Homo sapiens maltase-glucoamylase ...   131   4e-29
AB057452_1(AB057452|pid:none) Physcomitrella patens subsp. paten...   129   2e-28
(O43451) RecName: Full=Maltase-glucoamylase, intestinal; Include...   129   2e-28
AM430903_1(AM430903|pid:none) Vitis vinifera contig VV78X052784....   129   2e-28
AB006754_1(AB006754|pid:none) Coturnix japonica GAAII mRNA for a...   128   4e-28
EU955937_1(EU955937|pid:none) Zea mays clone 1553282 unknown mRNA.    126   1e-27
(Q653V7) RecName: Full=Probable alpha-glucosidase Os06g0675700; ...   125   3e-27

 psg: 1.02  gvh: 0.72  alm: 0.40  top: 0.27  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: vacuolar
	  8.0 %: cytoplasmic
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for CHR512 is exc

>CHR512F.Seq
GTAGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAA
TTATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTA
AATATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACAC
TTAACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAA
CTTTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAGAGATGGG
AAGTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAA
TTGAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTT
TATTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATT
ATTTANAATTAAGTACATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGGAGNNN
NNNNNNN

>CHR512Z.Seq
NNNNNNNNNNGGTGATCCAGGTGTTAGACCATTATTCTTTGAATATCCATCAGATCCAAA
TACTTTTGCAATTGATCAACAATTTTTAGTTGGTACAGGTTTAATGGTATCACCAGTTCT
CACTCAAGGTGCTACCACAGTGAATGCTTACTTCCCAAATGATATCTGGTATGAATATGG
TAATGGTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCTCCATTCGATGT
AATCAACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACCTCCTCATATGT
TACACCAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTACCATTTGAATT
GATTATTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATCCTTGGATGATGGTGA
ATCAATTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGATGTTGTCTCCTC
ACCATCTTCATCTGCCTACAAATTACAATCAACCATTCTCAATAACAATTATAATGGTAC
CGCTTCTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTTCAACAAGTCAT
TGTTAATGGTAGCCCAATCAATTCATTTAATGCTGTTTCNGATTCAACTCTCTCTGTTTC
AAATTTACAACTTGCTTTAGATGAATCCTTGAAGTNATTTGTATTATATAAAAAT

>CHR512P.Seq
GTAGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAA
TTATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTA
AATATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACAC
TTAACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAA
CTTTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAGAGATGGG
AAGTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAA
TTGAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTT
TATTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATT
ATTTANAATTAAGTACATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGGAG---
-------GGTGATCCAGGTGTTAGACCATTATTCTTTGAATATCCATCAGATCCAAATAC
TTTTGCAATTGATCAACAATTTTTAGTTGGTACAGGTTTAATGGTATCACCAGTTCTCAC
TCAAGGTGCTACCACAGTGAATGCTTACTTCCCAAATGATATCTGGTATGAATATGGTAA
TGGTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCTCCATTCGATGTAAT
CAACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACCTCCTCATATGTTAC
ACCAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTACCATTTGAATTGAT
TATTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATCCTTGGATGATGGTGAATC
AATTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGATGTTGTCTCCTCACC
ATCTTCATCTGCCTACAAATTACAATCAACCATTCTCAATAACAATTATAATGGTACCGC
TTCTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTTCAACAAGTCATTGT
TAATGGTAGCCCAATCAATTCATTTAATGCTGTTTCNGATTCAACTCTCTCTGTTTCAAA
TTTACAACTTGCTTTAGATGAATCCTTGAAGTNATTTGTATTATATAAAAAT