>CHR435 (CHR435Q) /CSM/CH/CHR4-B/CHR435Q.Seq.d/
ATTTTATTATATTTAAAAATTATTTTTAAATTATATCTATAAAATTATAAATTTTTTTAA
AGAATTAGTCATTTTATGCCAATTGGAAATAACAAAGATAACGATGGAACTACCCTGAAT
ATCTCAGGGTTAACCATCTCTGATAAGAGTTCGTCAACTTCATCAAACAACAACACAACA
ACAACAACAACACAAACTAGTGAACCATATGTCCCACCATCTCGTAGAAACAAGATGAAT
AATAGTTCAGGTTCATCAAGAGACGATTATCCATCACCATCTGACCGTGATAATCGTGAT
TCACCATTCAATCGTGACCGTCGTGACGATGGTTACAGAGGTGGTAACAATTTTAGGGAA
AATAACTCTAATAACAACAACAACAGTAGAGATAACTACAAAAGTCCATCATTCTCAAGA
AACAATAATAGTAATGGTGGTGGTAGTAGTAGTGGTAGCGGTGGTTGGGATAATGGTCCA
AGAGAACAAGGCCCACCAAGAGGTAATAGTTACTCTTATTCACCAAATAATTCATTCTCT
CGTGGTGGTAATCAAGGTGGTTATGGTAATAGAGGCGGAAGTGGCAGTAGTTTTGGTAXX
XXXXXXXXTCGTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAATCAAAG
AAATTTCCCAACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACT
TCAATCTTTCCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGG
TCTCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATAT
CTACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCCTT
CTTCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAA
TCAAGATATTCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAAGGTCC
ATCAAACTCAAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGAGA
TGATAACCGTGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAACCG
TAATGATAGAAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAACAA
TGGTGGTTCATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAATAAAT
CCATTTAATTCAATA

illylkiifklyl*nykff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTT
TTTTQTSEPYVPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRE
NNSNNNNNSRDNYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFS
RGGNQGGYGNRGGSGSSFG---

---VETKRSCDTLTYFLNQRNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDIASRG
LHIGNVNLVINFDLPTDIHIYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLMKASN
QDIPDWFEKMVHNLRMSKGPSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGYSNNR
NDRREDKSDYSMHHPYFSNNGGSYN*tftschsfnkk**ihliq

Frame A:
illylkiifklyl*nykff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTT
TTTTQTSEPYVPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRE
NNSNNNNNSRDNYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFS
RGGNQGGYGNRGGSGSSFG---

---slklnvlvillptf*ikeisqlpvfmvi*lnqkekmhfnlsvllqlhs**qlilhqe
vstsvmliwlsistclptyistfivlvepeeqekkv*qfpssmkrinqlvlnf*n**klq
ikifqiglkrwytisvclkvhqtqnqivhstnltivitiemitvvvmvvvvvvmevtvtt
vmieekinqitqcithtlatmvvhiikhsphvirliknnksi*fn

Frame B:
fyyi*klflnyiykiinffkelvilcqleitkitmelp*isqg*pslirvrqlhqtttqq
qqqhklvnhmshhlvetr*iivqvhqetiihhhltviivihhsivtvvtmvtevvtilgk
itlittttveittkvhhsqetiivmvvvvvvvavvgimvqenkahqevivtlihqiihsl
vvvikvvmvieaevavvlv---

---r*n*tfl*ysyllfkskkfpnylysw*fnstrkrkctsifpffcnsilssn*ycikr
sphr*c*fgyqfrlayrhtylrssyw*nrksrkkrfsnfllq*ke*tswc*tfeinesfk
srysrlv*kdgtqspyv*rsiklkik*siqqilq*ssq*r**pwwlwwwwww*wrlq*qp
***krr*irllnaspil*qqwwfi*lnihlmsfv**kiinpfnsi

Frame C:
fiifknyf*iisikl*iflkn*sfyanwk*qr*rwnypeylrvnhl**efvnfikqqhnn
nnntn**ticptis*kqde**frfikrrlsiti*p**s*ftiqs*ps*rwlqrw*qf*gk
*l**qqqq*r*lqksiilkkq***www***w*rwlg*wskrtrptkr**lllftk*fils
ww*srwlw**rrkwq*fw---

---VETKRSCDTLTYFLNQRNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDIASRG
LHIGNVNLVINFDLPTDIHIYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLMKASN
QDIPDWFEKMVHNLRMSKGPSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGYSNNR
NDRREDKSDYSMHHPYFSNNGGSYN*tftschsfnkk**ihliq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ404 (VHQ404Q) /CSM/VH/VHQ4-A/VHQ404Q.Seq.d/                        910   0.0
VHP734 (VHP734Q) /CSM/VH/VHP7-B/VHP734Q.Seq.d/                        910   0.0
VHP681 (VHP681Q) /CSM/VH/VHP6-D/VHP681Q.Seq.d/                        910   0.0
VHP588 (VHP588Q) /CSM/VH/VHP5-D/VHP588Q.Seq.d/                        910   0.0
VHP118 (VHP118Q) /CSM/VH/VHP1-A/VHP118Q.Seq.d/                        910   0.0
VHO596 (VHO596Q) /CSM/VH/VHO5-D/VHO596Q.Seq.d/                        910   0.0
VHM740 (VHM740Q) /CSM/VH/VHM7-B/VHM740Q.Seq.d/                        910   0.0
VHL839 (VHL839Q) /CSM/VH/VHL8-B/VHL839Q.Seq.d/                        910   0.0
VHL166 (VHL166Q) /CSM/VH/VHL1-C/VHL166Q.Seq.d/                        910   0.0
VHK893 (VHK893Q) /CSM/VH/VHK8-D/VHK893Q.Seq.d/                        910   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL731714|AL731714.9 Mouse DNA sequence from clone RP23-117H24 on chromosome 11 Contains  the gene for a novel protein (4933427E13Rik).    48   1e-08  3
AC117147|AC117147.4 Rattus norvegicus clone CH230-398H18, WORKING DRAFT SEQUENCE, 1 ordered piece.    56   0.002  1
BX248512|BX248512.14 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-284D12.    30   0.004  3
AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    38   0.005  2
BQ576817|BQ576817.1 PfESToab10b04.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:YAJE_SCHPO Q09911 HYPOTHETICAL 59.0 KDA PROTEIN C30D11.14C IN CHROMOSOME I. [1] ;, mRNA sequence.    38   0.006  2
AC115579|AC115579.3 Dictyostelium discoideum chromosome 2 map 6097680-6105897 strain AX4, complete sequence.    54   0.007  1
U23476|U23476.1 Dictyostelium discoideum phosphatidylinositol-4,5-diphosphate 3-kinase (PIK1) mRNA, complete cds.    54   0.007  1
AY160096|AY160096.1 Dictyostelium discoideum nucleotide exchange factor RasGEF G (gefG) gene, complete cds.    32   0.018  6
AC125555|AC125555.5 Rattus norvegicus clone CH230-7L23, WORKING DRAFT SEQUENCE, 3 unordered pieces.    36   0.018  2
CB389495|CB389495.1 OSTF118B11_1 AD-wrmcDNA Caenorhabditis elegans cDNA, mRNA sequence.    52   0.029  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QS3) RecName: Full=Probable ATP-dependent RNA helicase ddx3;...   310   7e-83
(Q6Z4K6) RecName: Full=DEAD-box ATP-dependent RNA helicase 52B; ...   156   1e-36
(Q75HJ0) RecName: Full=DEAD-box ATP-dependent RNA helicase 37;  ...   152   2e-35
EU953399_1(EU953399|pid:none) Zea mays clone 1406878 unknown mRNA.    152   3e-35
(Q2R1M8) RecName: Full=DEAD-box ATP-dependent RNA helicase 52C; ...   150   1e-34
AY062446_1(AY062446|pid:none) Arabidopsis thaliana ATP-dependent...   149   2e-34
AM048814_1(AM048814|pid:none) Platynereis dumerilii mRNA for PL1...   149   2e-34
AM444101_1(AM444101|pid:none) Vitis vinifera contig VV78X103110....   149   3e-34
AP008212_1481(AP008212|pid:none) Oryza sativa (japonica cultivar...   148   4e-34
(Q0DB53) RecName: Full=DEAD-box ATP-dependent RNA helicase 52A; ...   148   4e-34

 psg: 0.88  gvh: 0.39  alm: 0.46  top: 0.30  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CHR435 is cyt

>CHR435F.Seq
ATTTTATTATATTTAAAAATTATTTTTAAATTATATCTATAAAATTATAAATTTTTTTAA
AGAATTAGTCATTTTATGCCAATTGGAAATAACAAAGATAACGATGGAACTACCCTGAAT
ATCTCAGGGTTAACCATCTCTGATAAGAGTTCGTCAACTTCATCAAACAACAACACAACA
ACAACAACAACACAAACTAGTGAACCATATGTCCCACCATCTCGTAGAAACAAGATGAAT
AATAGTTCAGGTTCATCAAGAGACGATTATCCATCACCATCTGACCGTGATAATCGTGAT
TCACCATTCAATCGTGACCGTCGTGACGATGGTTACAGAGGTGGTAACAATTTTAGGGAA
AATAACTCTAATAACAACAACAACAGTAGAGATAACTACAAAAGTCCATCATTCTCAAGA
AACAATAATAGTAATGGTGGTGGTAGTAGTAGTGGTAGCGGTGGTTGGGATAATGGTCCA
AGAGAACAAGGCCCACCAAGAGGTAATAGTTACTCTTATTCACCAAATAATTCATTCTCT
CGTGGTGGTAATCAAGGTGGTTATGGTAATAGAGGCGGAAGTGGCAGTAGTTTTGGTANN
NNNNNNNN

>CHR435Z.Seq
NNNNNNNNNNTCGTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAATCAA
AGAAATTTCCCAACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCA
CTTCAATCTTTCCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGA
GGTCTCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACAT
ATCTACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCC
TTCTTCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCA
AATCAAGATATTCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAAGGT
CCATCAAACTCAAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGA
GATGATAACCGTGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAAC
CGTAATGATAGAAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAAC
AATGGTGGTTCATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAATAA
ATCCATTTAATTCAATA

>CHR435P.Seq
ATTTTATTATATTTAAAAATTATTTTTAAATTATATCTATAAAATTATAAATTTTTTTAA
AGAATTAGTCATTTTATGCCAATTGGAAATAACAAAGATAACGATGGAACTACCCTGAAT
ATCTCAGGGTTAACCATCTCTGATAAGAGTTCGTCAACTTCATCAAACAACAACACAACA
ACAACAACAACACAAACTAGTGAACCATATGTCCCACCATCTCGTAGAAACAAGATGAAT
AATAGTTCAGGTTCATCAAGAGACGATTATCCATCACCATCTGACCGTGATAATCGTGAT
TCACCATTCAATCGTGACCGTCGTGACGATGGTTACAGAGGTGGTAACAATTTTAGGGAA
AATAACTCTAATAACAACAACAACAGTAGAGATAACTACAAAAGTCCATCATTCTCAAGA
AACAATAATAGTAATGGTGGTGGTAGTAGTAGTGGTAGCGGTGGTTGGGATAATGGTCCA
AGAGAACAAGGCCCACCAAGAGGTAATAGTTACTCTTATTCACCAAATAATTCATTCTCT
CGTGGTGGTAATCAAGGTGGTTATGGTAATAGAGGCGGAAGTGGCAGTAGTTTTGGTA--
--------TCGTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAATCAAAG
AAATTTCCCAACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACT
TCAATCTTTCCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGG
TCTCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATAT
CTACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCCTT
CTTCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAA
TCAAGATATTCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAAGGTCC
ATCAAACTCAAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGAGA
TGATAACCGTGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAACCG
TAATGATAGAAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAACAA
TGGTGGTTCATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAATAAAT
CCATTTAATTCAATA