>CHR416 (CHR416Q) /CSM/CH/CHR4-A/CHR416Q.Seq.d/
XXXXXXXXXXGTGGTTATTATAAAGATGAGGAGAAAACTANAGAAGAATTCAAANATGGT
TGGTTCCANANTGGTGANATTGGTCGTTGGAATNGTGATGGTAGTTTTAGCATTGTTGAT
NGTAAAAAAAANATNTTTAAATTATTTCAAGGTGAATATGTTGCAGTTGAAAANATTGAA
ACAATAGTTGTAAANAGTGAATACGTTGAACAAGTTTGNATTTANGGTGATTTTCAAAAA
TCATGNGTAATTGCAATCATTNATCCACATCCANAATCATGTANTGAANGGGNTGGTTNA
AANAAAACTGATAAANACATTAAANAAATTTGTAAAAATCAANATTTTATCAAAGTTGTA
TTGGATGATATAATTAAAAATTGCAAAAAATTTGGTTTTCANGGATTTGAAATTNCAAAA
NCAATTCATTTAACTCCANAAGCATT

---GYYKDEEKTXEEFKXGWFXXGXIGRWNXDGSFSIVDXKKXXFKLFQGEYVAVEXIET
IVVXSEYVEQVXIXGDFQKSXVIAIIXPHPXSCXEXXGXXKTDKXIKXICKNQXFIKVVL
DDIIKNCKKFGFXGFEIXKXIHLTPXA

Frame A:
---wll*r*genxrriqxwlvpxw*xwslex*w*f*hc*x*kkxx*iisr*iccs*kx*n
nsckx*ir*tslxlx*fskimxncnhxstsximx*xgwxkxn**xh*xnl*ksxfyqsci
g*yn*klqkiwfsxi*nxkxnsfnsxsi


Frame B:
---GYYKDEEKTXEEFKXGWFXXGXIGRWNXDGSFSIVDXKKXXFKLFQGEYVAVEXIET
IVVXSEYVEQVXIXGDFQKSXVIAIIXPHPXSCXEXXGXXKTDKXIKXICKNQXFIKVVL
DDIIKNCKKFGFXGFEIXKXIHLTPXA


Frame C:
---viikmrrklxknsxmvgsxxvxlvvgxvmvvlallxvkkxxlnyfkvnmlqlkxlkq
*l*xvntlnkfxfxvifknhx*lqsxihixnhvxxgxvxxklixtlxkfvkixilsklyw
mi*lkiaknlvfxdlkxqxqfi*lxkh

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHR416 (CHR416Q) /CSM/CH/CHR4-A/CHR416Q.Seq.d/                        609   e-173
CHS265 (CHS265Q) /CSM/CH/CHS2-C/CHS265Q.Seq.d/                        587   e-167
CHO281 (CHO281Q) /CSM/CH/CHO2-D/CHO281Q.Seq.d/                        585   e-166
VFM341 (VFM341Q) /CSM/VF/VFM3-B/VFM341Q.Seq.d/                        569   e-161
CFI593 (CFI593Q) /CSM/CF/CFI5-D/CFI593Q.Seq.d/                        569   e-161
CFI815 (CFI815Q) /CSM/CF/CFI8-A/CFI815Q.Seq.d/                        561   e-159
AHR169 (AHR169Q) /CSM/AH/AHR1-C/AHR169Q.Seq.d/                        557   e-158
VHD490 (VHD490Q) /CSM/VH/VHD4-D/VHD490Q.Seq.d/                        553   e-157
VHG380 (VHG380Q) /CSM/VH/VHG3-D/VHG380Q.Seq.d/                        551   e-156
AHI311 (AHI311Q) /CSM/AH/AHI3-A/AHI311Q.Seq.d/                        549   e-155

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY196478|AY196478.1 Dictyostelium discoideum fatty acyl-CoA synthetase (fcsA) mRNA, complete cds.    58   7e-08  2
BQ875501|BQ875501.1 QGI8E20.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGI8E20, mRNA sequence.    54   0.003  1
BQ870927|BQ870927.1 QGI10G21.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGI10G21, mRNA sequence.    54   0.003  1
CZ968225|CZ968225.1 167785 Tomato MboI BAC Library Lycopersicon esculentum genomic clone SL_MboI0024M22 5, genomic survey sequence.    48   0.15   1
BU014148|BU014148.1 QGJ6J03.yg.ab1 QG_EFGHJ lettuce serriola Lactuca serriola cDNA clone QGJ6J03, mRNA sequence.    46   0.61   1
DR997967|AC007504_12 Putative acyl CoA synthetase [Arabidopsis thaliana], mRNA sequence.    46   0.61   1
CV081897|CV081897.1 Mdfrt3069a06.y1 Mdfrt Malus x domestica cDNA clone Mdfrt3069a06 5' similar to TR:Q9XIA9 Q9XIA9 PUTATIVE ACYL COA SYNTHETASE. ;, mRNA sequence.    46   0.61   1
CO905293|CO905293.1 Mdfrt3065p05.y1 Mdfrt Malus x domestica cDNA clone Mdfrt3065p05 5' similar to TR:Q9XIA9 Q9XIA9 PUTATIVE ACYL COA SYNTHETASE. ;, mRNA sequence.    46   0.61   1
CO900973|CO900973.1 Mdfrt3061h08.y1 Mdfrt Malus x domestica cDNA clone Mdfrt3061h08 5' similar to TR:Q9XIA9 Q9XIA9 PUTATIVE ACYL COA SYNTHETASE. ;, mRNA sequence.    46   0.61   1
CO867562|CO867562.1 Mddb5010k22.y1 Mddb Malus x domestica cDNA clone Mddb5010k22 5' similar to TR:Q9XIA9 Q9XIA9 PUTATIVE ACYL COA SYNTHETASE. ;, mRNA sequence.    46   0.61   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ174259_1(DQ174259|pid:none) Gossypium hirsutum ACS-like protei...    67   1e-10
BC077120_1(BC077120|pid:none) Danio rerio zgc:101071, mRNA (cDNA...    64   1e-09
AP008207_2021(AP008207|pid:none) Oryza sativa (japonica cultivar...    64   2e-09
AP003212_15(AP003212|pid:none) Oryza sativa Japonica Group genom...    64   2e-09
BC046740_1(BC046740|pid:none) Xenopus laevis similar to fatty ac...    64   2e-09
BC043756_1(BC043756|pid:none) Xenopus laevis fatty acid Coenzyme...    64   2e-09
BC076898_1(BC076898|pid:none) Xenopus tropicalis acyl-CoA synthe...    64   2e-09
T07928(T07928) probable long-chain-fatty-acid-CoA ligase (EC 6.2...    63   3e-09
AK006541_1(AK006541|pid:none) Mus musculus adult male testis cDN...    63   4e-09
(Q8JZR0) RecName: Full=Long-chain-fatty-acid--CoA ligase 5;     ...    63   4e-09

 psg: 0.75  gvh: 0.36  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for CHR416 is cyt

>CHR416Z.Seq
NNNNNNNNNNGTGGTTATTATAAAGATGAGGAGAAAACTANAGAAGAATTCAAANATGGT
TGGTTCCANANTGGTGANATTGGTCGTTGGAATNGTGATGGTAGTTTTAGCATTGTTGAT
NGTAAAAAAAANATNTTTAAATTATTTCAAGGTGAATATGTTGCAGTTGAAAANATTGAA
ACAATAGTTGTAAANAGTGAATACGTTGAACAAGTTTGNATTTANGGTGATTTTCAAAAA
TCATGNGTAATTGCAATCATTNATCCACATCCANAATCATGTANTGAANGGGNTGGTTNA
AANAAAACTGATAAANACATTAAANAAATTTGTAAAAATCAANATTTTATCAAAGTTGTA
TTGGATGATATAATTAAAAATTGCAAAAAATTTGGTTTTCANGGATTTGAAATTNCAAAA
NCAATTCATTTAACTCCANAAGCATT